Difference between revisions of "Team:DTU-Denmark/Composite Part"
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Latest revision as of 02:33, 22 October 2019
Composite parts
Type: Measurement
Size: 1505 bp
This is a device for testing promoters by expressing mCherry.
Assembly Compatibility:
Type: Measurement
Size: 10725 bp
This plasmid is designed as a shuttle vector to test different fungal promoters
Assembly Compatibility:
Type: Composite
Size: 1324 bp
This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.
Assembly Compatibility:
Type: Composite
Size: 5546 bp
This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.
Assembly Compatibility:
Type: Measurement
Size: 7139 bp
This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.
Assembly Compatibility:
