Difference between revisions of "Team:DTU-Denmark/Composite Part"

 
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<h2>Composite parts</h2>
 
<h2>Composite parts</h2>
 
 
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<p><div>We made composite parts </div></p>
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<p><div></div></p>
 
</div>
 
</div>
 
</div><!-- /end col-md-4 -->
 
</div><!-- /end col-md-4 -->
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<section class="grey_con">
 
<section class="grey_con">
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  <div class="container">
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    <div class="container">
  
  
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<div class="interlabspace">
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<H2 style="text-align: left;margin-bottom:20px;"></H2>
  
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<!--HELENA HERE-->
 
  
 +
<!--#############################################################################################
 +
Fungal MoClo promoter test device
 +
##############################################################################################-->
 +
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046009"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046009</button></a><button class="collapsiblebig">Fungal MoClo promoter test device</button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Measurement</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>1505 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This is a device for testing promoters by expressing mCherry.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/56/T--DTU-Denmark--fung_moclo.jpg" alt="The figure shows the components of the fungal MoClo promoter test device" style="max-width: 280px;"> 
 +
</p>
 +
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/be/T--DTU-Denmark--fungmoclo_comp.jpg" alt="The figure shows the compatibility of the fungal MoClo promoter test device" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 +
<!--#############################################################################################
 +
Promoter test plasmid pAMA1-zing_Pyr
 +
##############################################################################################-->
 +
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046021</button></a><button class="collapsiblebig">Promoter test plasmid pAMA1-zing_Pyr</button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Measurement</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>10725 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This plasmid is designed as a shuttle vector to test different fungal promoters</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1e/T--DTU-Denmark--Ama_pyr.jpg" alt="The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr" style="max-width: 215px;"> 
 +
</p>
 +
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/83/T--DTU-Denmark--Ama_pyrcomp.jpg" alt="Compatibility of promoter test plasmid pAMA1-zing_Pyr" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 +
 +
<!--#############################################################################################
 +
PyrG selection marker from <i>A. fumigatus</i>
 +
##############################################################################################-->
 +
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from <i>A. fumigatus</i></button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Composite</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>1324 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 145px;">
 +
</p>
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 +
 +
 +
<!--#############################################################################################
 +
pGIA2P1 insert
 +
##############################################################################################-->
 +
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046030"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046030</button></a><button class="collapsiblebig">pGIA2P1 insert</button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Composite</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>5546 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4b/T--DTU-Denmark--pGIA.jpg" alt="The figure shows the 5546 basepair long pGIA2P1 insert" style="max-width: 230px;"> 
 +
</p>
 +
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pGIA2P1 insert" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 +
 +
<!--#############################################################################################
 +
pPEA2P1 insert
 +
##############################################################################################-->
 +
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046031"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046031</button></a><button class="collapsiblebig">pPEA2P1 insert</button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Measurement</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>7139 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e6/T--DTU-Denmark--pPEA.jpg" alt="The figure shows the 7139 basepair long pPEA2P1 insert" style="max-width: 425px;" >
 +
</p>
 +
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pPEA2P1 insert" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
  
  
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</div></div>
 
</div></div>
  
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<script>
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 +
 +
var coll = document.getElementsByClassName("collapsiblebig");
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var i;
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for (i = 0; i < coll.length; i++) {
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  coll[i].addEventListener("click", function() {
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    this.classList.toggle("coactivebleu");
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    var content = this.nextElementSibling;
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    if (content.style.maxHeight){
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      content.style.maxHeight = null;
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    } else {
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      content.style.maxHeight = content.scrollHeight + "px";
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    }
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  });
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}
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</script>
  
 
</html>
 
</html>

Latest revision as of 02:33, 22 October 2019

Composite parts

Type: Measurement

Size: 1505 bp

This is a device for testing promoters by expressing mCherry.

The figure shows the components of the fungal MoClo promoter test device

Assembly Compatibility:

The figure shows the compatibility of the fungal MoClo promoter test device

Type: Measurement

Size: 10725 bp

This plasmid is designed as a shuttle vector to test different fungal promoters

The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr

Assembly Compatibility:

Compatibility of promoter test plasmid pAMA1-zing_Pyr

Type: Composite

Size: 1324 bp

This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.

The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>

Assembly Compatibility:

Compatibility of PyrG selection marker from <i>A. fumigatus</i>

Type: Composite

Size: 5546 bp

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

The figure shows the 5546 basepair long pGIA2P1 insert

Assembly Compatibility:

Compatibility of pGIA2P1 insert

Type: Measurement

Size: 7139 bp

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

The figure shows the 7139 basepair long pPEA2P1 insert

Assembly Compatibility:

Compatibility of pPEA2P1 insert
The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience