Difference between revisions of "Team:JiangnanU China/Parts"

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{{JiangnanU_China}}
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{{:Team:JiangnanU_China/Header}}
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<html>
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<html xmlns="http://www.w3.org/1999/html">
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<head>
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<div class="column full_size">
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        #HQ_page h1, h2, h3, h4, h5 {
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<h1>Parts</h1>
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            margin: 0;
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. The iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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            padding: 0;
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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        }
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</div>
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<div class="column full_size">
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        #HQ_page p {
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<div class="highlight decoration_background">
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            font-size: inherit;
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<h3>Note</h3>
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            font-family: inherit;
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<p>Note that parts must be well documented on each part's <a href="http://parts.igem.org/Main_Page">Main Page on the Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
+
        }
−
This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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</div>
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        /*字体*/
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        @font-face {
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            font-family: 'Futura_Bold';
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            src: url('https://static.igem.org/mediawiki/2019/e/e0/T--JiangnanU_China--ziti_Futura_Bold.otf');
 +
        }
  
 +
        @font-face {
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            font-family: 'FuturaFuturisC-Italic';
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            src: url('https://static.igem.org/mediawiki/2019/4/49/T--JiangnanU_China--FuturaFuturisC-Italic.otf');
 +
        }
  
 +
        @font-face {
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            font-family: 'Futura-Medium-6';
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            src: url('https://static.igem.org/mediawiki/2019/1/11/T--JiangnanU_China--Futura-Medium-6.otf');
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        }
  
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        .fb_48 {
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            font-family: Futura_Bold, sans-serif;
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<h3>Adding parts to the registry</h3>
+
        .fb_72 {
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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            font-family: Futura_Bold, sans-serif;
 +
            font-size: 4em;
 +
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−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Documentation includes the characterization data of your parts.</p>
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        .fm_22 {
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<div class="button_link">
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            font-family: Futura-Medium-6, sans-serif;
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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            font-size: 1.3em;
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ADD PARTS
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        }
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</a>
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</div>
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        .contents {
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<h3>Inspiration</h3>
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            margin: 5% 15% 15%;
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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        }
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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        /*一排*/
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<ul>
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        .row {
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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            display: flex;
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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            flex-direction: row;
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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            margin: 0;
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</ul>
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        }
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</div>
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        /*一列*/
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        .column {
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            margin: 0% 15% 15%;
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        .centers {
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    <style>
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        .bgd {
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            background-image: url('https://static.igem.org/mediawiki/2019/5/59/T--JiangnanU_China--parts_0.png');
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            background-size: 100% 100%;
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            background-position: center;
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            background-repeat: no-repeat;
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<body>
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<!--首页-->
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<div class="bgd" id="head">
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    <div class="split_small"></div>
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    <div class="split"></div>
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    <div class="contents" style="color: white">
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        <div class="column">
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            <div class="centers">
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                <div class="fb_72">
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                    <b>Parts</b>
 +
                </div>
 +
                <div style="height: 60vh"></div>
 +
                <!--                View more-->
 +
                <a href="#phage" style="text-decoration: none">
 +
                    <div class="row" style="align-content: center;color: white;">
 +
                        <img
 +
                                src="https://static.igem.org/mediawiki/2019/0/09/T--JiangnanU_China--host_liubianxing.png"
 +
                                alt="back" style="width: 6%;height:6%;">
 +
                        <div class="fb_48" style="margin-left: 2%;margin-top: 1%">View all</div>
 +
                    </div>
 +
                </a>
 +
 
 +
            </div>
 +
        </div>
 +
    </div>
 
</div>
 
</div>
  
 +
<!--第一部分-->
 +
<div class="split"></div>
 +
<div class="contents" id="phage">
 +
    <div class="column">
 +
        <div class="centers">
 +
            <div class="fb_72">
 +
                <b>Basic Parts</b>
 +
            </div>
 +
            <!--            BBa_K3137000-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137000 : <a href="http://parts.igem.org/Part:BBa_K3137000" style="color:blue;"alt="">P<i>rsmH</i></a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                250bp constitutive promoter<br/>
 +
                We ligated promoter <i>rsmH</i> with <i>gfp</i> to measure the fluorescence intensity of GFP.
 +
            </div>
 +
          <!--            BBa_K3137001-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137001 : <a href="http://parts.igem.org/Part:BBa_K3137001" style="color:blue;"alt="">Improved RBS 4</a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                11bp RBS<br/>
 +
                As we all know, RBS refers to a sputum-rich untranslated region upstream of the initiation codon AUG.
 +
                <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0033" style="color:blue;"alt="">BBa_B0033</a> is a weaker RBS based on Ron Weiss thesis.
 +
                Compared with <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0030" style="color:blue;"alt="">BBa_B0030</a>,
 +
              <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0031" style="color:blue;"alt="">BBa_B0031</a>,
 +
              <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0032" style="color:blue;"alt="">BBa_B0032</a>,
 +
              <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0034" style="color:blue;"alt="">BBa_B0034</a>,
 +
              BBa_B0033 is the weakest,
 +
                and whose RBS strength is 0.35% of BBa_B0034. By analyzing their sequences, we found that BBa_B0033 has fewer purines and no not have AAA sequence.
 +
                SO we designed the RBS by adjusting the proportion of bases.
 +
            <br/>
 +
              Then we ligated the two RBS with promotor <i>rsmH</i> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K3137000" style="color:blue;"alt="">BBa_K3137000</a>)
 +
            and gene <i>gfp</i> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K3137011" style="color:blue;"alt="">BBa_K3137011</a>) and transform into <i>E. coli</i> BL21 to
 +
              compare their green fluorescence. And take <i>E. coli</i> BL21 as a control.
 +
                The figure below shows that <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K3137001" style="color:blue;"alt="">BBa_K3137001</a>
 +
                has the stronger fluorescently
 +
              protein expression than BBa_B0033, that means it’s efficient to increase the protein expression by increasing the number of purines in the RBS sequence,
 +
              which is beneficial to the binding of ribosomes.
 +
            </div>
 +
        <!--            BBa_K3137002-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137002 : <a href="http://parts.igem.org/Part:BBa_K3137002" style="color:blue;"alt="">P<i>putA</i></a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                250bp inducible promoter<br/>
 +
                P<i>putA</i> is an inducible promoter. When <i>E.coli</i> is infected by a phage, both report and
 +
                response gene
 +
                circuits are induced to express at the same time. When a phage infects <i>Escherichia coli</i> into the
 +
              latent period (about 5 min), P<i>putA</i> will induce the bacteria to express anti-protein and
 +
                display
 +
                green fluorescence.
 +
            </div>
  
−
<div class="clear extra_space"></div>
+
            <!--            BBa_K3137003-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137003 : <a href="http://parts.igem.org/Part:BBa_K3137003" style="color:blue;"alt=""> P<i>glcF </i> </a></b></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                250bp inducible promoter<br/>
 +
                P<i>glcF</i> is an inducible promoter that works when phages infect bacterial for around 20 min. If the
 +
                anti-proteins successfully resist phage infection, the inducible promoter P<i>glcF</i> will not
 +
                induce the
 +
                expression of downstream gene. If the anti-proteins cannot kill phages, the phages will continue to
 +
                infect. When phages infect bacteria into the burst period (about 20 min), the inducible promoter
 +
                P<i>glcF</i> will induce expression of the red fluorescent protein gene <i>mCherry</i> and the
 +
                downstream toxic
 +
                protein gene <i>protegrin-1</i>, which will make the bacteria display red fluorescence and lyse cells
 +
                before
 +
                the assembly of phages.
 +
            </div>
  
 +
            <!--            BBa_K3137004-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137004 : <a href="http://parts.igem.org/Part:BBa_K3137004" style="color:blue;"alt=""><i>mCherry</i></a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                708bp reporter gene<br/>
 +
                The <i>mCherry</i> can express red fluorescent protein and it is used as a reporter gene in the process of
 +
                reporting the number of cells.
 +
            </div>
  
 +
            <!--            BBa_K3137005-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137005 : <a href="http://parts.igem.org/Part:BBa_K3137005" style="color:blue;"alt=""><i>gntR</i></a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                996bp<br/>
 +
                One of the most abundant and widely distributed groups of Helix-turn-helix (HTH) transcription factors is the metabolite-responsive GntR family of regulators. These proteins contain a DNA-binding HTH domain at the N-terminus of the protein and an effector-binding and/or oligomerisation domain at the C-terminus, where upon on binding an effector molecule, a conformational change occurs in the protein which influences the DNA-binding properties of the regulator resulting in repression or activation of transcription[1].
 +
            </div>
  
 +
            <!--            BBa_K3137006-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137006 : <a href="http://parts.igem.org/Part:BBa_K3137006" style="color:blue;"alt=""><i>abpAB</i></a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                3803bp anti-protein<br/>
 +
                Gene <i>abpAB</i> is from <i>E.coli</i> genome which express anti-proteins that are resistant to T2,T4,T7
 +
                and λ phages. We obtained <i>abpA</i> and <i>abpB</i> by PCR from the genome of <i>E.coli</i> BL21 and
 +
                constructed
 +
                recombinant plasmid that connected <i>abpA</i> and <i>abpB</i> at the same time. Then we used IPTG to
 +
                induce the
 +
                expression of anti-protein and verified the resistant function of the protein.[2]
 +
            </div>
  
−
<div class="column full_size">
+
            <!--            BBa_K3137007-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137007 : <a href="http://parts.igem.org/Part:BBa_K3137007" style="color:blue;"alt=""><i>rzpD</i> </a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                462bp<br/>
 +
                Overexpression of <i>rzpD</i> causes an abnormal biofilm architecture.
 +
            </div>
  
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
            <!--            BBa_K3137008-->
−
<p>The information needed to initially create a part on the Registry is:</p>
+
            <div class="split_small"></div>
−
<ul>
+
            <div class="fb_48">
−
<li>Part Name</li>
+
                <b>BBa_K3137008 : <a href="http://parts.igem.org/Part:BBa_K3137008" style="color:blue;"alt=""><i>yhjH</i> </a></b>
−
<li>Part type</li>
+
            </div>
−
<li>Creator</li>
+
            <br/>
−
<li>Sequence</li>
+
            <div class="fm_22">
−
<li>Short Description (60 characters on what the DNA does)</li>
+
                <i>yhjH</i>       768bp<br/>
−
<li>Long Description (Longer description of what the DNA does)</li>
+
                <i>YhjH</i> protein contains a EAL domain to catalyze c-di-GMP into GMP, so as to regulate the levels of
−
<li>Design considerations</li>
+
                c-di-GMP.
−
</ul>
+
            </div>
  
−
<p>
+
            <!--            BBa_K3137009-->
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137009 : <a href="http://parts.igem.org/Part:BBa_K3137009" style="color:blue;"alt=""><i>nuoE</i></a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                501bp<br/>
 +
                <i>NuoE</i> is part of the soluble fragment of NADH dehydrogenase I, which represents the electron input
 +
                part
 +
                of the enzyme.
 +
            </div>
  
−
</div>
+
            <!--            BBa_K3137010-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137010 : <a href="http://parts.igem.org/Part:BBa_K3137010" style="color:blue;"alt="">T7 terminator</a></b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
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                48bp terminator<br/>
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                T7 terminator is used to quantify the level of expression in <i>E. coli</i>.
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            </div>
  
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            <!--            BBa_K3137011-->
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                <b>BBa_K3137011 : <a href="http://parts.igem.org/Part:BBa_K3137011" style="color:blue;"alt=""><i>gfp</i></a></b>
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            </div>
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            <br/>
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            <div class="fm_22">
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                717bp reporter gene<br/>
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                The <i>gfp</i> can express green fluorescent protein, which can be used as a reporter gene in the process
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                of reporting the number of cells.
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            </div>
  
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<h3>Part Table </h3>
 
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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            <!--第二部分-->
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            <div class="fb_72"><b>Composite Part</b></div>
  
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<groupparts>iGEM19 JiangnanU_China</groupparts>
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                <b>BBa_K3137013 : <a href="http://parts.igem.org/Part:BBa_K3137013" style="color:blue;"alt="">report circuit</a></b>
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            <div class="fm_22">
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            1666bp
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            </div>
  
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            <img src="https://static.igem.org/mediawiki/2019/1/19/T--JiangnanU_China--parts_1.png"
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                style="width: 100%;height: auto;" alt="1666bp">
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            <div class="fm_22">
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The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA and a green fluorescent protein gene <i>gfp</i>. In burst period (about 20 min), there is an inducible promoter PglcF, and a red fluorescent protein gene <i>mCherry</i>.
  
 +
When <i>E. coli</i> is infected by a phage, report gene circuit is induced to express. When a phage infects <i>E. coli</i> into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to expressthe green fluorescent protein gene <i>gfp</i>.
  
 +
If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF will activate the red fluorescent protein gene <i>mCherry</i>. so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.
  
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            </div>
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            <div class="split_small"></div>
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                <b>BBa_K3137014 : <a href="http://parts.igem.org/Part:BBa_K3137014" style="color:blue;"alt="">response circuit</a></b>
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            </div>
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            <br/>
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            <div class="fm_22">
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            5097bp
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            </div>
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            <div class="split_small"></div>
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            <img src="https://static.igem.org/mediawiki/2019/f/fb/T--JiangnanU_China--parts_2.png"
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                style="width: 100%;height: auto;" alt="5097bp">
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            <div class="split_small"></div>
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            <div class="fm_22">
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                The responce gene circuit is composed of an inducible promoter PputA and anti-phage protein genes of the incubation period (about 5 min), and an inducible promoter PglcF and a toxic protein gene protegrin-1 of the burst period (about 20 min).
 +
 +
When <i>E. coli</i> is infected by a phage, responce gene circuit is induced to express. When a phage infects <i>E. coli</i> into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant proteins.
 +
 +
If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF will activate the expression of downstream toxic protein gene so that the bacteria will lyse cells before the assembly of phages.
 +
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            </div>
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            <img src="https://static.igem.org/mediawiki/2019/b/bb/T--JiangnanU_China--parts--12138.png"
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            <img src="https://static.igem.org/mediawiki/2019/8/81/T--JiangnanU_China--parts_2000.png"
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                style="width: 100%;height: auto">
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<div class="fm_22">
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                References[1-2]
 +
                <br/>
 +
                <br/>
 +
                [1] Hoskisson P A , Sébastien Rigali. Chapter 1: Variation in form and function the helix-turn-helix regulators of the GntR superfamily[J]. Advances in applied microbiology, 2009, 69(69):1-22.
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                <br />         
 +
[2] Yasui R, Washizaki A, Furihata Y, Yonesaki T, Otsuka Y: AbpA and AbpB provide anti-phage activity in <i>Escherichia coli</i>. Genes Genet Syst 2014, 89(2):51-60.
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{{:Team:JiangnanU_China/Footer}}

Latest revision as of 02:26, 22 October 2019

JiangNan

Basic Parts
BBa_K3137000 : PrsmH

250bp constitutive promoter
We ligated promoter rsmH with gfp to measure the fluorescence intensity of GFP.
BBa_K3137001 : Improved RBS 4

11bp RBS
As we all know, RBS refers to a sputum-rich untranslated region upstream of the initiation codon AUG. BBa_B0033 is a weaker RBS based on Ron Weiss thesis. Compared with BBa_B0030, BBa_B0031, BBa_B0032, BBa_B0034, BBa_B0033 is the weakest, and whose RBS strength is 0.35% of BBa_B0034. By analyzing their sequences, we found that BBa_B0033 has fewer purines and no not have AAA sequence. SO we designed the RBS by adjusting the proportion of bases.
Then we ligated the two RBS with promotor rsmH (BBa_K3137000) and gene gfp (BBa_K3137011) and transform into E. coli BL21 to compare their green fluorescence. And take E. coli BL21 as a control. The figure below shows that BBa_K3137001 has the stronger fluorescently protein expression than BBa_B0033, that means it’s efficient to increase the protein expression by increasing the number of purines in the RBS sequence, which is beneficial to the binding of ribosomes.
BBa_K3137002 : PputA

250bp inducible promoter
PputA is an inducible promoter. When E.coli is infected by a phage, both report and response gene circuits are induced to express at the same time. When a phage infects Escherichia coli into the latent period (about 5 min), PputA will induce the bacteria to express anti-protein and display green fluorescence.
BBa_K3137003 : PglcF

250bp inducible promoter
PglcF is an inducible promoter that works when phages infect bacterial for around 20 min. If the anti-proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the anti-proteins cannot kill phages, the phages will continue to infect. When phages infect bacteria into the burst period (about 20 min), the inducible promoter PglcF will induce expression of the red fluorescent protein gene mCherry and the downstream toxic protein gene protegrin-1, which will make the bacteria display red fluorescence and lyse cells before the assembly of phages.
BBa_K3137004 : mCherry

708bp reporter gene
The mCherry can express red fluorescent protein and it is used as a reporter gene in the process of reporting the number of cells.
BBa_K3137005 : gntR

996bp
One of the most abundant and widely distributed groups of Helix-turn-helix (HTH) transcription factors is the metabolite-responsive GntR family of regulators. These proteins contain a DNA-binding HTH domain at the N-terminus of the protein and an effector-binding and/or oligomerisation domain at the C-terminus, where upon on binding an effector molecule, a conformational change occurs in the protein which influences the DNA-binding properties of the regulator resulting in repression or activation of transcription[1].
BBa_K3137006 : abpAB

3803bp anti-protein
Gene abpAB is from E.coli genome which express anti-proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time. Then we used IPTG to induce the expression of anti-protein and verified the resistant function of the protein.[2]
BBa_K3137007 : rzpD

462bp
Overexpression of rzpD causes an abnormal biofilm architecture.
BBa_K3137008 : yhjH

yhjH 768bp
YhjH protein contains a EAL domain to catalyze c-di-GMP into GMP, so as to regulate the levels of c-di-GMP.
BBa_K3137009 : nuoE

501bp
NuoE is part of the soluble fragment of NADH dehydrogenase I, which represents the electron input part of the enzyme.
BBa_K3137010 : T7 terminator

48bp terminator
T7 terminator is used to quantify the level of expression in E. coli.
BBa_K3137011 : gfp

717bp reporter gene
The gfp can express green fluorescent protein, which can be used as a reporter gene in the process of reporting the number of cells.
Composite Part
BBa_K3137013 : report circuit

1666bp
1666bp
The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA and a green fluorescent protein gene gfp. In burst period (about 20 min), there is an inducible promoter PglcF, and a red fluorescent protein gene mCherry. When E. coli is infected by a phage, report gene circuit is induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to expressthe green fluorescent protein gene gfp. If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF will activate the red fluorescent protein gene mCherry. so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.
BBa_K3137014 : response circuit

5097bp
5097bp
The responce gene circuit is composed of an inducible promoter PputA and anti-phage protein genes of the incubation period (about 5 min), and an inducible promoter PglcF and a toxic protein gene protegrin-1 of the burst period (about 20 min). When E. coli is infected by a phage, responce gene circuit is induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant proteins. If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF will activate the expression of downstream toxic protein gene so that the bacteria will lyse cells before the assembly of phages.
References[1-2]

[1] Hoskisson P A , Sébastien Rigali. Chapter 1: Variation in form and function the helix-turn-helix regulators of the GntR superfamily[J]. Advances in applied microbiology, 2009, 69(69):1-22.
[2] Yasui R, Washizaki A, Furihata Y, Yonesaki T, Otsuka Y: AbpA and AbpB provide anti-phage activity in Escherichia coli. Genes Genet Syst 2014, 89(2):51-60.
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