Difference between revisions of "Team:DTU-Denmark/Parts"

This is a strong promoter for Aspergillus niger that has high activity in the exponential phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.

Assembly Compatibility:

This is a medium strength constitutive promoter for Aspergillus niger. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. [1] This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression.

Assembly Compatibility:

This is a strong constitutive promoter for Aspergillus niger that has especially high activity in the lag phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.

Assembly Compatibility:

This is a medium strength constitutive promoter for Aspergillus niger. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. The consensus promoter was expected to have high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.

Assembly Compatibility:

This is a very strong constitutive promoter for Aspergillus niger that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the mstA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the mstA promoters in Aspergillus and is expected to have a high constitutive expression.

Assembly Compatibility:

This is a weak constitutive promoter for Aspergillus niger. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on an unknown promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the An07g08210 promoters in Aspergillus and is expected to have a weak constitutive expression.

Assembly Compatibility:

This is a strong promoter for Aspergillus niger that has high activity in the late exponential phase and stationary phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.

Assembly Compatibility:

This is a strong promoter for Aspergillus niger that has high activity in the stationary growth phase. This is a synthetic stationary phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the hfbD promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This is a consensus promoter and are expected to have high expression in the stationary phase.

Assembly Compatibility:

This is a device for testing promoters by expressing mCherry.

Assembly Compatibility:

This is a promoter for Aspergillus niger of unknown strength that is expected to have high expression in the exponential growth phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.

Assembly Compatibility:

This is a promoter for Aspergillus niger of unknown strength that is expected to have constitutive expression. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression

Assembly Compatibility:

This is a very strong constitutive promoter for Aspergillus niger that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.

Assembly Compatibility:

This is a promoter for Aspergillus niger of unknown strength that should be active in the stationary phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. [1] This version is a consensus sequence of an unknown gene's promoters in Aspergillus.

Assembly Compatibility:

This is a promoter on unknown for Aspergillus niger that is expected to have high activity in the late exponential phase and stationary phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.

Assembly Compatibility:

This part is the bacterial promoter BBa_R0010 with MoClo level 0 compatible overhangs.

Assembly Compatibility:

Transcriptional terminator from Aspergillus nidulans. Domesticated to be in compliance with RFC[1000]

Assembly Compatibility:

Protein secretion tag from the GlaA enzyme from Aspergillus niger.

Assembly Compatibility:

This part is a superfolded, monomeric, stable green fluorescent protein.

Assembly Compatibility:

This plasmid is designed as a shuttle vector to test different fungal promoters

Assembly Compatibility:

This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.

Assembly Compatibility:

This is a plasmid containing an E.coli ORI, an ampicillin selection marker, and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.

Assembly Compatibility:

This sequence is used for creating autonomously replicating plasmids to use for Aspergillus spp.

Assembly Compatibility:

CThis coding sequence from Aspergillus fumigatus is encoding Orotidine 5'-phosphate decarboxylase.

Assembly Compatibility:

Promoter from the PyrG selection marker gene from Aspergillus fumigatus

Assembly Compatibility:

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

Assembly Compatibility:

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

Assembly Compatibility:

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the downstream sequence BBa_K3046033).

Assembly Compatibility:

This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).

Assembly Compatibility: