Helenautzon (Talk | contribs) |
Helenautzon (Talk | contribs) |
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(63 intermediate revisions by 3 users not shown) | |||
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− | <div class=" topimg pull-right sm-no-float col-md- | + | <div class=" topimg pull-right sm-no-float col-md-7 "> |
− | <img src="https://static.igem.org/mediawiki/2019/ | + | <img src="https://static.igem.org/mediawiki/2019/3/31/T--DTU-Denmark--partsoverview2.svg" alt="Illutration of a puzzle of fungal parts" style="margin-top:75px;max-width:70%;margin-right:auto; margin-left:auto;display: block; |
height:auto;z-index:5;"> | height:auto;z-index:5;"> | ||
Line 38: | Line 38: | ||
− | <div class="pull-left col-md- | + | <div class="pull-left col-md-5 sm-text-center com-sm-12 "> |
− | <div class="team-overview"> | + | <div class="team-overview" style="font-size:1em";> |
<h2>Parts overview</h2> | <h2>Parts overview</h2> | ||
− | <p>< | + | <div><p>Through this project we have created a library of <i>Aspergillus</i> promoters and other fungal parts, which will make it easier for future iGEM-teams to work with filamentous fungi. These, along with other parts we have created or characterized, are described below.</p></div> |
</div> | </div> | ||
</div><!-- /end col-md-4 --> | </div><!-- /end col-md-4 --> | ||
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<div class="row flex-center sm-no-flex interlabspace"> | <div class="row flex-center sm-no-flex interlabspace"> | ||
<div class="column full_size"> | <div class="column full_size"> | ||
− | |||
− | |||
<div class="interlabspace"> | <div class="interlabspace"> | ||
Line 100: | Line 98: | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p>This is a synthetic | + | <p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the exponential phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1a/T--DTU-Denmark--glaA.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_2" style="max-width: | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1a/T--DTU-Denmark--glaA.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_2" style="max-width: 721px;" > |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/d/db/T--DTU-Denmark--glaAcomp.jpg" alt="The figure shows the compatibility of PLEAPglaA_2" </div></div> | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/d/db/T--DTU-Denmark--glaAcomp.jpg" alt="The figure shows the compatibility of PLEAPglaA_2" style="max-width: 170px;" > </div></div> |
</div> | </div> | ||
</div> | </div> | ||
− | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPsonB_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046002"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046002</button></a><button class="collapsiblebig">PLEAPsonB_1</button> |
Line 128: | Line 125: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>842 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a medium strength constitutive promoter for <i>Aspergillus niger</i>. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. [1] This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/8/88/T--DTU-Denmark--sonB.jpg" alt="The figure shows 842 basepair long promoter PLEAPsonB_1" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a1/T--DTU-Denmark--sonB_comp.jpg" alt="The figure shows the compatibility of promoter PLEAPsonB_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
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<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPgpdA_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046003"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046003</button></a><button class="collapsiblebig">PLEAPgpdA_1</button> |
Line 156: | Line 153: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Regulatory</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>926 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the lag phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression. |
+ | |||
+ | </p> | ||
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--gpdA.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_1" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/6/6b/T--DTU-Denmark--gpdA_comp.jpg" alt="The figure shows the compatibility of PLEAPgpdA_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPgpdA_2 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046004"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046004</button></a><button class="collapsiblebig">PLEAPgpdA_2</button> |
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</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>926 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p>This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project</p> | + | <p>This is a medium strength constitutive promoter for <i>Aspergillus niger</i>. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/3/3d/T--DTU-Denmark--gdpA2.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_2" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c6/T--DTU-Denmark--gdpA2comp.jpg" alt="The figure shows the compatibility of PLEAPgpdA_2" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
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<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPmstA_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046005"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046005</button></a><button class="collapsiblebig">PLEAPmstA_1</button> |
Line 222: | Line 222: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>986 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p>This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project</p> | + | <p>This is a very strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the mstA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the mstA promoters in Aspergillus and is expected to have a high constitutive expression.</p> |
+ | |||
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e8/T--DTU-Denmark--mstA.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_1" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/6/66/T--DTU-Denmark--mstA_comp.jpg" alt="The figure shows the compatibility of PLEAPmstA_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
− | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPunk_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046006"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046006</button></a><button class="collapsiblebig">PLEAPunk_1</button> |
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</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>933 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p>This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project</p> | + | <p>This is a weak constitutive promoter for <i>Aspergillus niger</i>. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on an unknown promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the An07g08210 promoters in Aspergillus and is expected to have a weak constitutive expression.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/6f/T--DTU-Denmark--unk1.jpg" alt="The figure shows the 933 basepair long promoter PLEAPunk_1" style="max-width: 721px;" > |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/3/34/T--DTU-Denmark--unk1_comp.jpg" alt="The figure shows the compatibility of PLEAPunk_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPgfaA_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046007"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046007</button></a><button class="collapsiblebig">PLEAPgfaA_1</button> |
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</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>973 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the late exponential phase and stationary phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus</i> niger. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--gfaA1.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_1" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/01/T--DTU-Denmark--gfaA_1comp.jpg" alt="The figure shows the compatibility of PLEAPgfaA_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
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<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPhfbD_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046008"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046008</button></a><button class="collapsiblebig">PLEAPhfbD_1</button> |
Line 318: | Line 319: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>1014 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the stationary growth phase. This is a synthetic stationary phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the hfbD promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This is a consensus promoter and are expected to have high expression in the stationary phase.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/93/T--DTU-Denmark--hfbD.jpg" alt="The figure shows the 1014 basepair long promoter PLEAPhfbD_1" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/92/T--DTU-Denmark--hfbDcomp.jpg" alt="The figure shows the compatibility of PLEAPhfbD_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | Fungal MoClo promoter test device | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046009"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046009</button></a><button class="collapsiblebig">Fungal MoClo promoter test device</button> |
Line 345: | Line 348: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Measurement</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>1505 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a device for testing promoters by expressing mCherry.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/56/T--DTU-Denmark--fung_moclo.jpg" alt="The figure shows the components of the fungal MoClo promoter test device" style="max-width: 280px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/be/T--DTU-Denmark--fungmoclo_comp.jpg" alt="The figure shows the compatibility of the fungal MoClo promoter test device" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
Line 366: | Line 369: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPglaA_1 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046010"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046010</button></a><button class="collapsiblebig">PLEAPglaA_1</button> |
Line 380: | Line 383: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>683 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a promoter for <i>Aspergillus niger</i> of unknown strength that is expected to have high expression in the exponential growth phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/2/23/T--DTU-Denmark--glaA1.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_1" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/5a/T--DTU-Denmark--glaA_1comp.jpg" alt="The figure shows the compatibility of PLEAPglaA_1" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
Line 397: | Line 400: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPsonB_2 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046012</button></a><button class="collapsiblebig">PLEAPsonB_2</button> |
Line 411: | Line 414: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>842 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a promoter for <i>Aspergillus niger</i> of unknown strength that is expected to have constitutive expression. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/14/T--DTU-Denmark--sonB_2.jpg" alt="The figure shows the 842 basepair long promoter PLEAPsonB_2" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/9e/T--DTU-Denmark--sinB_2comp.jpg" alt="The figure shows the compatibility of PLEAPsonB_2" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
Line 429: | Line 432: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPmstA_2 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046013</button></a><button class="collapsiblebig">PLEAPmstA_2</button> |
Line 443: | Line 446: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>986 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a very strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--mstA21.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_2" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/d/d7/T--DTU-Denmark--mstA21comp.jpg" alt="The figure shows the compatibility of PLEAPmstA_2" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPunk_4 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046014</button></a><button class="collapsiblebig">PLEAPunk_4</button> |
Line 474: | Line 478: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>724 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a promoter for Aspergillus niger of unknown strength that should be active in the stationary phase. |
+ | This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. [1] This version is a consensus sequence of an unknown gene's promoters in Aspergillus.</p> | ||
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5d/T--DTU-Denmark--unk4.jpg" alt="The figure shows the 724 basepair long promoter PLEAPunk_4" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/b2/T--DTU-Denmark--unk4_comp.jpg" alt="The figure shows the compatibility of PLEAPunk_4" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
Line 492: | Line 497: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PLEAPgfaA_2 | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046015</button></a><button class="collapsiblebig">PLEAPgfaA_2</button> |
Line 506: | Line 511: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>973 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This is a promoter on unknown for Aspergillus niger that is expected to have high activity in the late exponential phase and stationary phase. |
+ | This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.</p> | ||
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gfaA_2.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_2" style="max-width: 721px;" > |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/54/T--DTU-Denmark--gfaA_2comp.jpg" alt="The figure shows the compatibility of PLEAPgfaA_2" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | MoClo promoter stand-in | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046017"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046017</button></a><button class="collapsiblebig">MoClo promoter stand-in</button> |
Line 537: | Line 544: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>238 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This part is the bacterial promoter <a href="http://parts.igem.org/Part:BBa_R0010" target="_blank">BBa_R0010</a> with MoClo level 0 compatible overhangs.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/59/T--DTU-Denmark--moclo.jpg" alt="The figure shows the 238 basepair long MoClo promoter stand-in" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/e/eb/T--DTU-Denmark--moclocomp.jpg" alt="The figure shows the compatibility of the MoClo promoter stand-in" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | Ttef (Domesticated from <i>A. nidulans</i>) | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046018"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046018</button></a><button class="collapsiblebig">Ttef (Domesticated from <i>A. nidulans</i>)</button> |
Line 565: | Line 573: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Terminator</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>485 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>Transcriptional terminator from <i>Aspergillus nidulans</i>. Domesticated to be in compliance with RFC[1000]</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from <i>Aspergillus nidulans</i>)" style="max-width: 721px;" > |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/96/T--DTU-Denmark--ttefcomp.jpg" alt="The figure shows the compatibility of Ttef" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | Export signal from <i>A. niger</i> GlaA protein | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046019"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046019</button></a><button class="collapsiblebig">Export signal from <i>A. niger</i> GlaA protein</button> |
Line 596: | Line 605: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Tag</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>63 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>Protein secretion tag from the GlaA enzyme from <i>Aspergillus niger</i>.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from <i>A. niger</i> GlaA protein" style="max-width: 721px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/55/T--DTU-Denmark--GlaAPcomp.jpg" alt="Compatibility of export signal from <i>A. niger</i> GlaA protein" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | MoxGFP | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046020"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046020</button></a><button class="collapsiblebig">MoxGFP</button> |
Line 627: | Line 638: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Coding</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>720 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This part is a superfolded, monomeric, stable green fluorescent protein.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/67/T--DTU-Denmark--moxgfp.jpg" alt="The figure shows the 720 basepair long MoxGFP" style="max-width: 721px;" > |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/86/T--DTU-Denmark--moxGFP_comp.jpg" alt="Compatibility of MoxGFP" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | Promoter test plasmid pAMA1-zing_Pyr | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046021</button></a><button class="collapsiblebig">Promoter test plasmid pAMA1-zing_Pyr</button> |
Line 658: | Line 670: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Measurement</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>10725 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This plasmid is designed as a shuttle vector to test different fungal promoters</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1e/T--DTU-Denmark--Ama_pyr.jpg" alt="The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr" style="max-width: 215px;"> |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/83/T--DTU-Denmark--Ama_pyrcomp.jpg" alt="Compatibility of promoter test plasmid pAMA1-zing_Pyr" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PyrG selection marker from <i>A. fumigatus</i> | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from <i>A. fumigatus</i></button> |
Line 689: | Line 702: | ||
<div class="parttoppers"> | <div class="parttoppers"> | ||
<div class="typebox"> | <div class="typebox"> | ||
− | <p>Type: <b> | + | <p>Type: <b>Composite</b></p> |
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>1324 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 145px;"> |
+ | </p> | ||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 170px;"> </div></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <!--############################################################################################# | ||
+ | pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone | ||
+ | ##############################################################################################--> | ||
+ | |||
+ | |||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046023"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046023</button></a><button class="collapsiblebig">pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone</button> | ||
+ | |||
+ | |||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>Plasmid Backbone</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>7896 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="sizemargins"> | ||
+ | <p>This is a plasmid containing an <i>E.coli</i> ORI, an ampicillin selection marker, and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.</p> | ||
+ | |||
+ | |||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5f/T--DTU-Denmark--pAMA1_23.jpg" alt="The figure shows the 7896 basepair long pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone" style="max-width: 721px;" > | ||
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/8e/T--DTU-Denmark--pAma.jpg" alt="Compatibility of pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | <!--############################################################################################# | ||
+ | AMA1 sequence for autonomous replication in <i>Aspergillus</i> | ||
+ | ##############################################################################################--> | ||
+ | |||
+ | |||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046024"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046024</button></a><button class="collapsiblebig">AMA1 sequence for autonomous replication in <i>Aspergillus</i></button> | ||
+ | |||
+ | |||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>Coding</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>5721 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="sizemargins"> | ||
+ | <p>This sequence is used for creating autonomously replicating plasmids to use for <i>Aspergillus</i> spp.</p> | ||
+ | |||
+ | |||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 721px;" > | ||
+ | </p> | ||
+ | |||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c9/T--DTU-Denmark--AMA1.jpg" alt="Compatibility of AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 170px;"> </div></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
<!--############################################################################################# | <!--############################################################################################# | ||
− | + | PyrG CDS from <i>A. fumigatus</i> | |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
− | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part: | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046028"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046028</button></a><button class="collapsiblebig">PyrG CDS from <i>A. fumigatus</i> </button> |
Line 725: | Line 800: | ||
</div> | </div> | ||
<div class="sizebox"> | <div class="sizebox"> | ||
− | <p>Size: <b> | + | <p>Size: <b>906 bp</b></p> |
</div> | </div> | ||
</div> | </div> | ||
<div class="sizemargins"> | <div class="sizemargins"> | ||
− | <p> | + | <p>CThis coding sequence from <i>Aspergillus fumigatus</i> is encoding Orotidine 5'-phosphate decarboxylase.</p> |
− | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/ | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 721px;" |
</p> | </p> | ||
− | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/ | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gPyrGCDScomp.jpg" alt="Compatibility of PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
Line 742: | Line 817: | ||
+ | <!--############################################################################################# | ||
+ | PyrG promoter from <i>A. fumigatus</i> | ||
+ | ##############################################################################################--> | ||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046029"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046029</button></a><button class="collapsiblebig">PyrG promoter from <i>A. fumigatus</i> </button> | ||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>Regulatory</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>418 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="sizemargins"> | ||
+ | <p>Promoter from the PyrG selection marker gene from <i>Aspergillus fumigatus</i></p> | ||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/0/00/T--DTU-Denmark--PyrG29_gene.jpg" alt="The figure shows the 418 basepair long PyrG promoter from <i>Aspergillus fumigatus</i>" style="max-width: 721px;"> | ||
+ | </p> | ||
+ | |||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/03/T--DTU-Denmark--PyrG29.jpg" alt="Compatibility of PyrG promoter from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div> | ||
+ | |||
+ | </div> | ||
</div> | </div> | ||
+ | <!--############################################################################################# | ||
+ | pGIA2P1 insert | ||
+ | ##############################################################################################--> | ||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046030"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046030</button></a><button class="collapsiblebig">pGIA2P1 insert</button> | ||
+ | |||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>Composite</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>5546 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="sizemargins"> | ||
+ | <p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p> | ||
+ | |||
+ | |||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4b/T--DTU-Denmark--pGIA.jpg" alt="The figure shows the 5546 basepair long pGIA2P1 insert" style="max-width: 230px;"> | ||
+ | </p> | ||
+ | |||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pGIA2P1 insert" style="max-width: 170px;"> </div></div> | ||
+ | |||
</div> | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <!--############################################################################################# | ||
+ | pPEA2P1 insert | ||
+ | ##############################################################################################--> | ||
+ | |||
+ | |||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046031"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046031</button></a><button class="collapsiblebig">pPEA2P1 insert</button> | ||
+ | |||
+ | |||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>Measurement</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>7139 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="sizemargins"> | ||
+ | <p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p> | ||
+ | |||
+ | |||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e6/T--DTU-Denmark--pPEA.jpg" alt="The figure shows the 7139 basepair long pPEA2P1 insert" style="max-width: 425px;" > | ||
+ | </p> | ||
+ | |||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pPEA2P1 insert" style="max-width: 170px;"> </div></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <!--############################################################################################# | ||
+ | IS2 upstream sequence for <i>A. niger</i> genome integration | ||
+ | ##############################################################################################--> | ||
+ | |||
+ | |||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046032"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046032</button></a><button class="collapsiblebig">IS2 upstream sequence for <i>A. niger</i> genome integration</button> | ||
+ | |||
+ | |||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>DNA</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>2096 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="sizemargins"> | ||
+ | <p>This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the downstream sequence BBa_K3046033).</p> | ||
+ | |||
+ | |||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/90/T--DTU-Denmark--IS2up_gene.jpg" alt="The figure shows the 2096 basepair long IS2 upstream sequence for <i>A. niger</i> genome integration" style="max-width: 721px;" > | ||
+ | </p> | ||
+ | |||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a1/T--DTU-Denmark--IS2up.jpg" alt="Compatibility of IS2 upstream sequence for <i>A. niger</i> genome integration" style="max-width: 170px;"> </div></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <!--############################################################################################# | ||
+ | IS2 downstream sequence for <i>A. niger</i> genome integration | ||
+ | ##############################################################################################--> | ||
+ | |||
+ | |||
+ | <div class="buttonshadow"> | ||
+ | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046033"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046033</button></a><button class="collapsiblebig">IS2 downstream sequence for <i>A. niger</i> genome integration</button> | ||
+ | |||
+ | |||
+ | <div class="contentparts"> | ||
+ | <div class="parttoppers"> | ||
+ | <div class="typebox"> | ||
+ | <p>Type: <b>DNA</b></p> | ||
+ | </div> | ||
+ | <div class="sizebox"> | ||
+ | <p>Size: <b>2112 bp</b></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="sizemargins"> | ||
+ | <p>This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).</p> | ||
+ | |||
+ | |||
+ | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/d/de/T--DTU-Denmark--IS2dw.jpg" alt="The figure shows the 2112 basepair IS2 downstream sequence for <i>A. niger</i> genome integration" style="max-width: 721px;"> | ||
+ | </p> | ||
+ | |||
+ | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/4/41/T--DTU-Denmark--IS2dw_comp.jpg" alt="Compatibility of IS2 downstream sequence for <i>A. niger</i> genome integration" style="max-width: 170px;"> </div></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
Latest revision as of 02:21, 22 October 2019
Parts overview
Through this project we have created a library of Aspergillus promoters and other fungal parts, which will make it easier for future iGEM-teams to work with filamentous fungi. These, along with other parts we have created or characterized, are described below.