Difference between revisions of "Team:HK SSC/Collaborations"

 
(19 intermediate revisions by 2 users not shown)
Line 10: Line 10:
 
style='mso-spacerun:yes'>&nbsp;</span></span></p>
 
style='mso-spacerun:yes'>&nbsp;</span></span></p>
  
−
<p>With similar objectives on our projects, we are honoured to be able to collaborate with team SZTA_Szeged_HU. Their project aims to detect Microcystin toxin in waters using bioindicators. Here are some areas we collaborated on: </p>
+
<h3>SZTA_Szeged_HU</h3>
−
</br>
+
−
<h1>1. Providing us with their plasmid </h1>
+
  
 +
<p>With similar objectives on our projects, we are honoured to be able to collaborate with team SZTA_Szeged_HU. Their project aims to detect Microcystin toxin in waters using bioindicators. </br>
 +
<div class="center"><img style="width:60%; height:60%; margin: auto;" src="https://static.igem.org/mediawiki/2019/4/47/T--HK_SSC--image1.jpeg"/></div>
 +
<div class="center"><b>Fig1. Team SZTA_Szeged_HU</b></div>
 +
<br>
 +
<br>
  
 +
<p>Here are some areas we collaborated on: </p>
 +
<br>
  
  
 +
<h4>1. Providing us with their plasmid </h4>
 +
<p>Team SZTA_Szeged_HU has designed 2 plasmids for the detection of Microcystin toxin. Since our project aims to remove Microcystin, they provided us with their plasmids, so that we can conduct further detailed downstream analysis. In return, we will be giving them our experimental data. </p>
 +
<div class="center"><img style="width:60%; height:60%; margin: auto;" src="https://static.igem.org/mediawiki/2019/c/c0/T--HK_SSC--Hungary_gel_pic.png"/></div>
 +
<div class="center"><b>Fig2. Showing the correct band sizes of the plasmids provided by SZTA_Szeged_HU. The expected band size is around 3800bp. (Circular plasmid DNA often shows smaller band size than expected)</b></div>
 +
<div class="center"><img style="width:40%; height:40%; margin: auto;" src="https://static.igem.org/mediawiki/2019/f/fd/T--HK_SSC--Collab_plate1_equation.png"/></div>
 +
<div class="center"><b>Fig3. Successful transfromation of the 2 plasmid constructs</b></div></br>
 +
<p>However, we failed to excite the green fluorescent  protein in the diluted overnight cultures, possibly due to wrong wavelength (the GFP used in our project is different from that of team SZTA_Szeged_HU). We have refelected the experiment results. </p>
 +
 +
 +
 +
</br>
 +
<h4>2. Survey </h4>
 +
<p>We helped them collect data for their surveys. These surveys include interviewing fishpond owners and researchers about how algal blooms have affected them. </p>
 
</html>
 
</html>

Latest revision as of 02:15, 22 October 2019

HOME
TEAM
Team Members
Collaborations
PROJECT
Description
Design
Experiments
Model
Notebook
Contribution
Results
Demonstrate
Improve
Attributions
PARTS
Parts Overview
Basic Parts
Composite Parts
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM ⇗

Collaborations

 

SZTA_Szeged_HU

With similar objectives on our projects, we are honoured to be able to collaborate with team SZTA_Szeged_HU. Their project aims to detect Microcystin toxin in waters using bioindicators.

Fig1. Team SZTA_Szeged_HU


Here are some areas we collaborated on:


1. Providing us with their plasmid

Team SZTA_Szeged_HU has designed 2 plasmids for the detection of Microcystin toxin. Since our project aims to remove Microcystin, they provided us with their plasmids, so that we can conduct further detailed downstream analysis. In return, we will be giving them our experimental data.

Fig2. Showing the correct band sizes of the plasmids provided by SZTA_Szeged_HU. The expected band size is around 3800bp. (Circular plasmid DNA often shows smaller band size than expected)
Fig3. Successful transfromation of the 2 plasmid constructs

However, we failed to excite the green fluorescent protein in the diluted overnight cultures, possibly due to wrong wavelength (the GFP used in our project is different from that of team SZTA_Szeged_HU). We have refelected the experiment results.


2. Survey

We helped them collect data for their surveys. These surveys include interviewing fishpond owners and researchers about how algal blooms have affected them.