Difference between revisions of "Team:DUT China B/Parts"
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| + | <p>This year, DUT_CHINA_B submitted a total of 14 parts, involving signal peptides located in the eye spots, mutants of rhodopsin in the Chlamydomonas reinhardtii channel, and red light-controlled protein with split renilla luciferase. Among them, the most innovative design is BBa_K3061011, BBa_K3061012 which are improved from the old part BBa_K1159201, BBa_K1159201. After characterization, we found that the old part has low luminescence intensity. Using modeling scores, we predicted new cleavage sites and found that nanoluc splitting at new sites has higher luminescence intensity. This not only guides the design of split luciferase in the experiment, but also provides help and advice for similar work in the future.</p> | ||
<h1 style="font-family: 'Times New Roman' !important; "><a name="Basic Parts" >Basic Parts</a><img src="https://static.igem.org/mediawiki/2019/a/a9/T--DUT_China_B--partsbasic.svg" class="icon"> </h1> | <h1 style="font-family: 'Times New Roman' !important; "><a name="Basic Parts" >Basic Parts</a><img src="https://static.igem.org/mediawiki/2019/a/a9/T--DUT_China_B--partsbasic.svg" class="icon"> </h1> | ||
| − | <p style="font-family: 'Times New Roman' !important; "> | + | <p style="font-family: 'Times New Roman' !important; "><table class="table-fill"> |
| − | + | ||
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| − | + | ||
| − | + | ||
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| − | <table class="table-fill"> | + | |
<thead> | <thead> | ||
<tr> | <tr> | ||
<th class="text-left">Name</th> | <th class="text-left">Name</th> | ||
| − | <th class="text-left">Type</th> | + | <th class="text-left">Original BioBrick</th><th class="text-left">Type</th> |
<th class="text-left">Description</th> | <th class="text-left">Description</th> | ||
<th class="text-left">Designer</th> | <th class="text-left">Designer</th> | ||
| + | |||
</tr> | </tr> | ||
</thead> | </thead> | ||
<tbody class="table-hover"> | <tbody class="table-hover"> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061001">BBa_K3061001</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">None</td> |
| − | <td class="text-left"> | + | <td class="text-left">coding</td> |
| − | <td class="text-left"> | + | <td class="text-left">VchR, Channel rhodopsins of Volvox carteri</td> |
| + | <td class="text-left">Kaidi Chen</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061001">BBa_K3061002</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">None</td> |
| − | <td class="text-left"> | + | <td class="text-left">coding</td> |
| − | <td class="text-left"> | + | <td class="text-left">signal peptide of Channelrhodopsin-2 |
| + | in Chlamydomonas reinhardtii</td> | ||
| + | <td class="text-left">Yuanzhen Zhang</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061003">BBa_K3061003</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_J52008">BBa_J52008</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">reporter</td> |
| − | <td class="text-left"> | + | <td class="text-left">N-rluc, N-terminal part of split Renilla luciferase</td><td class="text-left">Kaidi Chen</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061004">BBa_K3061004</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_J52008">BBa_J52008</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">reporter</td> |
| − | <td class="text-left"> | + | <td class="text-left">C-rluc, C-terminal part of split Renilla luciferase</td><td class="text-left">Kaidi Chen</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061005 "> BBa_K3061005 </a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K2023009"> BBa_K2023009 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left">reporter</td> |
| − | <td class="text-left"> | + | <td class="text-left">N-nanoluc, N-terminal part of split Guassia luciferase</td><td class="text-left">Jinwei Zhu</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061006"> BBa_K3061006 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K2023009"> BBa_K2023009 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left">reporter</td> |
| − | <td class="text-left"> | + | <td class="text-left">C-nanoluc, C-terminal part of split Guassia luciferase</td><td class="text-left">Jinwei Zhu</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061007"> BBa_K3061007 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left"> <a href="http://parts.igem.org/Part: BBa_K3061001"> BBa_K3061001 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left">coding</td> |
| − | <td class="text-left"> | + | <td class="text-left">fusion VchR with green fluorescent protein Clover</td><td class="text-left">Kaidi Chen</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"> <a href="http://parts.igem.org/Part:BBa_K3061008"> BBa_K3061008 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061002"> BBa_K3061002 </a> </td> |
| − | <td class="text-left"> | + | <td class="text-left">coding</td> |
| − | <td class="text-left"> | + | <td class="text-left">fusion signal peptide ChR2 with green fluorescent protein Clover</td><td class="text-left">Kaidi Chen</td> |
</tr> | </tr> | ||
| + | |||
| + | </tbody> | ||
| + | </table> </p> | ||
| + | |||
| + | </div> | ||
| + | <div class="cart"> | ||
| + | <h1 style="font-family: 'Times New Roman' !important; "><a name="Composite Parts">Composite Parts</a><img src="https://static.igem.org/mediawiki/2019/a/af/T--DUT_China_B--partscomplex.svg" class="icon"> </h1> | ||
| + | |||
| + | <p style="font-family: 'Times New Roman' !important; "></p> | ||
| + | <p></p> | ||
| + | <p></p> | ||
| + | <table class="table-fill"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th class="text-left">Name</th> | ||
| + | <th class="text-left">Original BioBrick</th><th class="text-left">Type</th> | ||
| + | <th class="text-left">Description</th> | ||
| + | <th class="text-left">Designer</th> | ||
| + | |||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody class="table-hover"> | ||
| + | |||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061009">BBa_K3061009</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159103">BBa_K1159103</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">composite</td> |
| − | <td class="text-left"> | + | <td class="text-left">fusion N-rluc with N-terminal phytochrome interacting Factor 3 (pif3) </td><td class="text-left">Huan Liu</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061010">BBa_K3061010</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K80103"> BBa_K80103</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">composite</td> |
| − | <td class="text-left"> | + | <td class="text-left">fusion C-rluc with Phytochrome B(PhyB)</td><td class="text-left">Huan Liu</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061011">BBa_K3061011</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159201">BBa_K1159201</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">composite</td> |
| − | <td class="text-left"> | + | <td class="text-left">the combination of new N terminal of Guassia luciferase and Spytag</td><td class="text-left">Jinwei Zhu</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061012">BBa_K3061012</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159200">BBa_K1159200</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">composite</td> |
| − | <td class="text-left"> | + | <td class="text-left">the combination of new C terminal of Guassia luciferase and SpyCatcher</td><td class="text-left">Jinwei Zhu</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061013">BBa_K3061013</a></td> |
| − | <td class="text-left"> | + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159201"> BBa_K1159201</a></td> |
| − | <td class="text-left"> | + | <td class="text-left">composite</td> |
| − | <td class="text-left"> | + | <td class="text-left">the combination of N terminal of Guassia luciferase and Spytag</td><td class="text-left">Kaidi Chen</td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061014">BBa_K3061014</a></td> | ||
| + | <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159200">BBa_K1159200</a></td> | ||
| + | <td class="text-left">composite</td> | ||
| + | <td class="text-left">the combination of C terminal of Guassia luciferase and SpyCatcher</td><td class="text-left">Kaidi Chen</td> | ||
</tr> | </tr> | ||
| − | |||
</tbody> | </tbody> | ||
</table> | </table> | ||
</div> | </div> | ||
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<hr> | <hr> | ||
Latest revision as of 01:34, 22 October 2019
This year, DUT_CHINA_B submitted a total of 14 parts, involving signal peptides located in the eye spots, mutants of rhodopsin in the Chlamydomonas reinhardtii channel, and red light-controlled protein with split renilla luciferase. Among them, the most innovative design is BBa_K3061011, BBa_K3061012 which are improved from the old part BBa_K1159201, BBa_K1159201. After characterization, we found that the old part has low luminescence intensity. Using modeling scores, we predicted new cleavage sites and found that nanoluc splitting at new sites has higher luminescence intensity. This not only guides the design of split luciferase in the experiment, but also provides help and advice for similar work in the future.
Basic Parts
| Name | Original BioBrick | Type | Description | Designer |
|---|---|---|---|---|
| BBa_K3061001 | None | coding | VchR, Channel rhodopsins of Volvox carteri | Kaidi Chen |
| BBa_K3061002 | None | coding | signal peptide of Channelrhodopsin-2 in Chlamydomonas reinhardtii | Yuanzhen Zhang |
| BBa_K3061003 | BBa_J52008 | reporter | N-rluc, N-terminal part of split Renilla luciferase | Kaidi Chen |
| BBa_K3061004 | BBa_J52008 | reporter | C-rluc, C-terminal part of split Renilla luciferase | Kaidi Chen |
| BBa_K3061005 | BBa_K2023009 | reporter | N-nanoluc, N-terminal part of split Guassia luciferase | Jinwei Zhu |
| BBa_K3061006 | BBa_K2023009 | reporter | C-nanoluc, C-terminal part of split Guassia luciferase | Jinwei Zhu |
| BBa_K3061007 | BBa_K3061001 | coding | fusion VchR with green fluorescent protein Clover | Kaidi Chen |
| BBa_K3061008 | BBa_K3061002 | coding | fusion signal peptide ChR2 with green fluorescent protein Clover | Kaidi Chen |
Composite Parts
| Name | Original BioBrick | Type | Description | Designer |
|---|---|---|---|---|
| BBa_K3061009 | BBa_K1159103 | composite | fusion N-rluc with N-terminal phytochrome interacting Factor 3 (pif3) | Huan Liu |
| BBa_K3061010 | BBa_K80103 | composite | fusion C-rluc with Phytochrome B(PhyB) | Huan Liu |
| BBa_K3061011 | BBa_K1159201 | composite | the combination of new N terminal of Guassia luciferase and Spytag | Jinwei Zhu |
| BBa_K3061012 | BBa_K1159200 | composite | the combination of new C terminal of Guassia luciferase and SpyCatcher | Jinwei Zhu |
| BBa_K3061013 | BBa_K1159201 | composite | the combination of N terminal of Guassia luciferase and Spytag | Kaidi Chen |
| BBa_K3061014 | BBa_K1159200 | composite | the combination of C terminal of Guassia luciferase and SpyCatcher | Kaidi Chen |