Difference between revisions of "Team:DUT China B/Parts"

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{{DUT_China_B}}
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<html>
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{{Template:DUT_China_B/nav}}
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<html lang="en">
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<head>
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<meta charset="UTF-8">
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<title>Document</title>
  
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/*** Table Styles **/
  
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<div class="column full_size">
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<h1>Parts</h1>
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  background: white;
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. The iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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  border-radius:3px;
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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}
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<p>Note that parts must be well documented on each part's <a href="http://parts.igem.org/Main_Page">Main Page on the Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
+
−
This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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th:last-child {
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th.text-center {
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<div class="column two_thirds_size">
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th.text-right {
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<div class="highlight decoration_B_full">
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<h3>Adding parts to the registry</h3>
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td.text-left {
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
  text-align: left;
 +
}
  
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Documentation includes the characterization data of your parts.</p>
+
td.text-center {
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<div class="button_link">
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  text-align: center;
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
}
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ADD PARTS
+
−
</a>
+
−
</div>
+
  
−
</div>
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td.text-right {
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</div>
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</style>
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</head>
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<div class="column third_size">
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<body>
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<div class="highlight decoration_A_full">
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<div class="firstimg">
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<h3>Inspiration</h3>
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<img src="https://static.igem.org/mediawiki/2019/9/9a/T--DUT_China_B--partsbanner1.jpg" alt="parts">
−
<p>We have a created a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
</div>
−
 
+
<div id="sides">
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
<ul id="menu">
−
<ul>
+
        <li>
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
            <a href="#Basic Parts"><font size="5"  >Basic Parts</font></a>
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
        <div style="text-align: center; width: 100%; height:40px"></div>
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
        </li>
−
</ul>
+
      <br>
−
</div>
+
        <li>
−
</div>
+
            <a href="#Composite Parts"><font size="5" >Composite Parts</font></a>
−
 
+
      <div style="text-align: center; width: 100%; height:40px"></div>
−
 
+
      </li>
−
<div class="clear extra_space"></div>
+
      <br>
−
 
+
        <li>
−
 
+
            <a href="#Improve Parts"><font size="5"></font></a>
−
 
+
           
−
 
+
        </li>
−
<div class="column full_size">
+
      <br>
−
 
+
     
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
    </ul>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
</div>
−
<ul>
+
<div id="maintest">
−
<li>Part Name</li>
+
 
−
<li>Part type</li>
+
<div  class="cart">
−
<li>Creator</li>
+
<p>This year, DUT_CHINA_B submitted a total of 14 parts, involving signal peptides located in the eye spots, mutants of rhodopsin in the Chlamydomonas reinhardtii channel, and red light-controlled protein with split renilla luciferase. Among them, the most innovative design is BBa_K3061011, BBa_K3061012 which are improved from the old part BBa_K1159201, BBa_K1159201. After characterization, we found that the old part has low luminescence intensity. Using modeling scores, we predicted new cleavage sites and found that nanoluc splitting at new sites has higher luminescence intensity. This not only guides the design of split luciferase in the experiment, but also provides help and advice for similar work in the future.</p>
−
<li>Sequence</li>
+
              <h1  style="font-family: 'Times New Roman' !important; "><a name="Basic Parts" >Basic Parts</a><img src="https://static.igem.org/mediawiki/2019/a/a9/T--DUT_China_B--partsbasic.svg" class="icon"> </h1>
−
<li>Short Description (60 characters on what the DNA does)</li>
+
               
−
<li>Long Description (Longer description of what the DNA does)</li>
+
<p style="font-family: 'Times New Roman' !important;  "><table class="table-fill">
−
<li>Design considerations</li>
+
    <thead>
−
</ul>
+
    <tr>
−
 
+
      <th class="text-left">Name</th>
−
<p>
+
      <th class="text-left">Original BioBrick</th><th class="text-left">Type</th>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
      <th class="text-left">Description</th>
−
 
+
        <th class="text-left">Designer</th>
−
</div>
+
         
−
 
+
      </tr>
−
 
+
    </thead>
−
<div class="clear extra_space"></div>
+
    <tbody class="table-hover">
−
<div class="line_divider"></div>
+
    <tr>
−
<div class="clear extra_space"></div>
+
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061001">BBa_K3061001</a></td>
−
 
+
    <td class="text-left">None</td>
−
<div class="column full_size">
+
    <td class="text-left">coding</td>
−
<h3>Part Table </h3>
+
    <td class="text-left">VchR, Channel rhodopsins of Volvox carteri</td>
−
 
+
    <td class="text-left">Kaidi Chen</td>
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
      </tr>
−
 
+
    <tr>
−
</html>
+
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061001">BBa_K3061002</a></td>
−
<groupparts>iGEM19 DUT_China_B</groupparts>
+
    <td class="text-left">None</td>
−
<html>
+
    <td class="text-left">coding</td>
−
</div>
+
    <td class="text-left">signal peptide of Channelrhodopsin-2
 +
in Chlamydomonas reinhardtii</td>
 +
    <td class="text-left">Yuanzhen Zhang</td>
 +
      </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061003">BBa_K3061003</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_J52008">BBa_J52008</a></td>
 +
    <td class="text-left">reporter</td>
 +
    <td class="text-left">N-rluc, N-terminal part of split Renilla luciferase</td><td class="text-left">Kaidi Chen</td>
 +
      </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061004">BBa_K3061004</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_J52008">BBa_J52008</a></td>
 +
    <td class="text-left">reporter</td>
 +
    <td class="text-left">C-rluc, C-terminal part of split Renilla luciferase</td><td class="text-left">Kaidi Chen</td>
 +
      </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061005 "> BBa_K3061005  </a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K2023009"> BBa_K2023009    </a> </td>
 +
    <td class="text-left">reporter</td>
 +
    <td class="text-left">N-nanoluc, N-terminal part of split Guassia luciferase</td><td class="text-left">Jinwei Zhu</td>
 +
      </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061006">  BBa_K3061006  </a> </td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K2023009"> BBa_K2023009  </a> </td>
 +
    <td class="text-left">reporter</td>
 +
    <td class="text-left">C-nanoluc, C-terminal part of split Guassia luciferase</td><td class="text-left">Jinwei Zhu</td>
 +
      </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061007">  BBa_K3061007 </a> </td>
 +
    <td class="text-left"> <a href="http://parts.igem.org/Part: BBa_K3061001">  BBa_K3061001  </a> </td>
 +
    <td class="text-left">coding</td>
 +
    <td class="text-left">fusion VchR with green fluorescent protein Clover</td><td class="text-left">Kaidi Chen</td>
 +
      </tr>
 +
   
 +
<tr>
 +
    <td class="text-left"> <a href="http://parts.igem.org/Part:BBa_K3061008">  BBa_K3061008  </a> </td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061002">  BBa_K3061002 </a>  </td>
 +
    <td class="text-left">coding</td>
 +
    <td class="text-left">fusion signal peptide ChR2 with green fluorescent protein Clover</td><td class="text-left">Kaidi Chen</td>
 +
  </tr>
  
 +
    </tbody>
 +
    </table> </p>
 +
 +
              </div>
 +
              <div class="cart">
 +
              <h1 style="font-family: 'Times New Roman' !important; "><a name="Composite Parts">Composite Parts</a><img src="https://static.igem.org/mediawiki/2019/a/af/T--DUT_China_B--partscomplex.svg" class="icon"> </h1>
 +
               
 +
<p style="font-family: 'Times New Roman' !important; "></p>
 +
              <p></p>
 +
                <p></p>
 +
<table class="table-fill">
 +
    <thead>
 +
    <tr>
 +
      <th class="text-left">Name</th>
 +
      <th class="text-left">Original BioBrick</th><th class="text-left">Type</th>
 +
      <th class="text-left">Description</th>
 +
        <th class="text-left">Designer</th>
 +
         
 +
      </tr>
 +
    </thead>
 +
    <tbody class="table-hover">
 +
   
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061009">BBa_K3061009</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159103">BBa_K1159103</a></td>
 +
    <td class="text-left">composite</td>
 +
    <td class="text-left">fusion N-rluc with N-terminal phytochrome interacting Factor 3 (pif3) </td><td class="text-left">Huan Liu</td>
 +
  </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061010">BBa_K3061010</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K80103"> BBa_K80103</a></td>
 +
    <td class="text-left">composite</td>
 +
    <td class="text-left">fusion C-rluc with Phytochrome B(PhyB)</td><td class="text-left">Huan Liu</td>
 +
  </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061011">BBa_K3061011</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159201">BBa_K1159201</a></td>
 +
    <td class="text-left">composite</td>
 +
    <td class="text-left">the combination of  new N terminal of Guassia luciferase and Spytag</td><td class="text-left">Jinwei Zhu</td>
 +
  </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061012">BBa_K3061012</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159200">BBa_K1159200</a></td>
 +
    <td class="text-left">composite</td>
 +
    <td class="text-left">the combination of new C terminal of Guassia luciferase and SpyCatcher</td><td class="text-left">Jinwei Zhu</td>
 +
  </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061013">BBa_K3061013</a></td>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K1159201"> BBa_K1159201</a></td>
 +
    <td class="text-left">composite</td>
 +
    <td class="text-left">the combination of N terminal of Guassia luciferase and Spytag</td><td class="text-left">Kaidi Chen</td>
 +
  </tr>
 +
<tr>
 +
    <td class="text-left"><a href="http://parts.igem.org/Part:BBa_K3061014">BBa_K3061014</a></td>
 +
    <td class="text-left"><a  href="http://parts.igem.org/Part:BBa_K1159200">BBa_K1159200</a></td>
 +
    <td class="text-left">composite</td>
 +
    <td class="text-left">the combination of C terminal of Guassia luciferase and SpyCatcher</td><td class="text-left">Kaidi Chen</td>
 +
  </tr>
 +
    </tbody>
 +
    </table>
 +
</div>
 +
       
 +
<hr>
  
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Latest revision as of 01:34, 22 October 2019

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This year, DUT_CHINA_B submitted a total of 14 parts, involving signal peptides located in the eye spots, mutants of rhodopsin in the Chlamydomonas reinhardtii channel, and red light-controlled protein with split renilla luciferase. Among them, the most innovative design is BBa_K3061011, BBa_K3061012 which are improved from the old part BBa_K1159201, BBa_K1159201. After characterization, we found that the old part has low luminescence intensity. Using modeling scores, we predicted new cleavage sites and found that nanoluc splitting at new sites has higher luminescence intensity. This not only guides the design of split luciferase in the experiment, but also provides help and advice for similar work in the future.

Basic Parts

Name Original BioBrickType Description Designer
BBa_K3061001 None coding VchR, Channel rhodopsins of Volvox carteri Kaidi Chen
BBa_K3061002 None coding signal peptide of Channelrhodopsin-2 in Chlamydomonas reinhardtii Yuanzhen Zhang
BBa_K3061003 BBa_J52008 reporter N-rluc, N-terminal part of split Renilla luciferaseKaidi Chen
BBa_K3061004 BBa_J52008 reporter C-rluc, C-terminal part of split Renilla luciferaseKaidi Chen
BBa_K3061005 BBa_K2023009 reporter N-nanoluc, N-terminal part of split Guassia luciferaseJinwei Zhu
BBa_K3061006 BBa_K2023009 reporter C-nanoluc, C-terminal part of split Guassia luciferaseJinwei Zhu
BBa_K3061007 BBa_K3061001 coding fusion VchR with green fluorescent protein CloverKaidi Chen
BBa_K3061008 BBa_K3061002 coding fusion signal peptide ChR2 with green fluorescent protein CloverKaidi Chen

Composite Parts

Name Original BioBrickType Description Designer
BBa_K3061009 BBa_K1159103 composite fusion N-rluc with N-terminal phytochrome interacting Factor 3 (pif3) Huan Liu
BBa_K3061010 BBa_K80103 composite fusion C-rluc with Phytochrome B(PhyB)Huan Liu
BBa_K3061011 BBa_K1159201 composite the combination of new N terminal of Guassia luciferase and SpytagJinwei Zhu
BBa_K3061012 BBa_K1159200 composite the combination of new C terminal of Guassia luciferase and SpyCatcherJinwei Zhu
BBa_K3061013 BBa_K1159201 composite the combination of N terminal of Guassia luciferase and SpytagKaidi Chen
BBa_K3061014 BBa_K1159200 composite the combination of C terminal of Guassia luciferase and SpyCatcherKaidi Chen