Difference between revisions of "Team:DTU-Denmark/Parts"
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| − | <div><p> | + | <div><p>Through this project we have created a library of <i>Aspergillus</i> promoters and other fungal parts, which will make it easier for future iGEM-teams to work with filamentous fungi. These, along with other parts we have created or characterized, are described below.</p></div> |
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Revision as of 21:29, 21 October 2019
Parts overview
Through this project we have created a library of Aspergillus promoters and other fungal parts, which will make it easier for future iGEM-teams to work with filamentous fungi. These, along with other parts we have created or characterized, are described below.
Part Table
Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.
Type: Regulatory
Size: 683 bp
This is a strong promoter for Aspergillus niger that has high activity in the exponential phase.
Assembly Compatibility:
Type: Regulatory
Size: 842 bp
This is a medium strength constitutive promoter for Aspergillus niger.
Assembly Compatibility:
Type: Regulatory
Size: 926 bp
This is a strong constitutive promoter for Aspergillus niger that has especially high activity in the lag phase.
Assembly Compatibility:
Type: Regulatory
Size: 926 bp
This is a medium strength constitutive promoter for Aspergillus niger.
Assembly Compatibility:
Type: Regulatory
Size: 986 bp
This is a very strong constitutive promoter for Aspergillus niger that has especially high activity in the exponential growth phase.
Assembly Compatibility:
Type: Regulatory
Size: 933 bp
This is a weak constitutive promoter for Aspergillus niger.
Assembly Compatibility:
Type: Regulatory
Size: 973 bp
This is a strong promoter for Aspergillus niger that has high activity in the late exponential phase and stationary phase.
Assembly Compatibility:
Type: Regulatory
Size: 1014 bp
This is a strong promoter for Aspergillus niger that has high activity in the stationary growth phase.
Assembly Compatibility:
Type: Regulatory
Size: 683 bp
This is a promoter for Aspergillus niger of unknown strength that is expected to have high expression in the exponential growth phase.
Assembly Compatibility:
Type: Composite
Size: 1324 bp
This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.
Assembly Compatibility:
Type: Plasmid Backbone
Size: 7896 bp
This is a plasmid containing an E.coli ORI, an ampicillin selection marker, and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.
Assembly Compatibility:
Type: Coding
Size: 5721 bp
This sequence is used for creating autonomously replicating plasmids to use for Aspergillus spp.
Assembly Compatibility:
Type: Regulatory
Size: 418 bp
Promoter from the PyrG selection marker gene from Aspergillus fumigatus
Assembly Compatibility:
Type: Composite
Size: 5546 bp
This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.
Assembly Compatibility:
Type: Measurement
Size: 7139 bp
This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.
Assembly Compatibility:
Type: DNA
Size: 2096 bp
This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the downstream sequence BBa_K3046033).
Assembly Compatibility:
Type: DNA
Size: 2112 bp
This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).
Assembly Compatibility:
