Difference between revisions of "Team:JiangnanU China/Measurement"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Measurement</h1>
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<p>There are a lot of exciting parts in the Registry, but many parts have still not been characterized or need more characterization to make them more useful. Synthetic Biology needs great measurement approaches for characterizing parts, and efficient new methods for characterizing many parts at once. If you've done something exciting in the area of Measurement, describe it here!</p>
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<h3>Best Measurement Special Prize</h3>
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<p>If you've done excellent work in measurement, you should consider nominating your team for this special prize. Designing great measurement approaches for characterizing new or existing parts or developing and implementing an efficient new method for characterizing thousands of parts are good examples.
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To compete for the <a href="https://2019.igem.org/Judging/Awards">Best Measurement prize</a>, please describe your work on this page and also fill out the description on the <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a>.
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<br><br>
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You must also delete the message box on the top of this page to be eligible for this prize.
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<h3>Inspiration</h3>
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<p>You can look at what other teams did to get some inspiration! <br />
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Here are a few examples:</p>
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<li><a href="https://2018.igem.org/Team:UC_Davis/Measurement">2018 UC Davis</a></li>
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<li><a href="https://2017.igem.org/Team:TUDelft/Measurement">2017 TUDelft</a></li>
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<li><a href="https://2016.igem.org/Team:Stanford-Brown">2016 Stanford-Brown</a></li>
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<li><a href="https://2015.igem.org/Team:William_and_Mary">2015 William and Mary</a></li>
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<li><a href="https://2014.igem.org/Team:Aachen">2014 Aachen  </a></li>
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                    <b>Measurement</b>
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                        In 2018, the team iGEM18_SCAU-China chose a strong <i>E. coli</i> endogenous promoter
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                        (P<I>rplJ</i>，<a href="http://parts.igem.org/Part:BBa_K2559003">BBa_K2559003</a>) to express their
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                        amended eGFP (<a href="http://parts.igem.org/Part:BBa_K2559005">BBa_K2559005</a>). This year our team
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                        JiangnanU_China also chose this promoter P<I>rplJ</i> (BBa_K2559003) to express their amended eGFP
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                        (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength.
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                        However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h
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                        and 10 h.(with the help of <a href="https://2019.igem.org/Measurement/Resources"> Measurement
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                        Hub Resources</a>，Fig 3.，Fig 4.) Through the change of value
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                        what we measured, we can found the promoter P<I>rplJ</i> worked well. On the other hand, we observed
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                        the eGFP fluorescent protein by fluorescence microscope and we found that the <i>E. coli</i> with the
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                        amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.
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Latest revision as of 20:35, 21 October 2019

JiangNan

In 2018, the team iGEM18_SCAU-China chose a strong E. coli endogenous promoter (PrplJ，BBa_K2559003) to express their amended eGFP (BBa_K2559005). This year our team JiangnanU_China also chose this promoter PrplJ (BBa_K2559003) to express their amended eGFP (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength. However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h and 10 h.(with the help of Measurement Hub Resources，Fig 3.，Fig 4.) Through the change of value what we measured, we can found the promoter PrplJ worked well. On the other hand, we observed the eGFP fluorescent protein by fluorescence microscope and we found that the E. coli with the amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.
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