Difference between revisions of "Team:JiangnanU China/Measurement"

 
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                     <b>Measurement</b>
 
                     <b>Measurement</b>
 
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<Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/><Br/>
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                    <div class="fm_22" style="font-size: 1.2em">
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                        In 2018, the team iGEM18_SCAU-China chose a strong <i>E. coli</i> endogenous promoter
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                        (P<I>rplJ</i>，BBa_K2559003) to express their amended eGFP (BBa_K2559005). This year our team
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                        JiangnanU_China also chose this promoter P<I>rplJ</i> (BBa_K2559003) to express their amended eGFP
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                        (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength.
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                        However, we measured the value of (fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h
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                        and 10 h.(with the help of <a href="https://2019.igem.org/Measurement/Resources"> Measurement
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                        Hub Resources</a>，Fig 3.，Fig 4.) Through the change of value
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                        what we measured, we can found the promoter P<I>rplJ</i> worked well. In the other hand, we observed
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                        the eGFP fluorescent protein by fluorescence microscope and we found that the <i>E. coli</i> with the
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                        amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.
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                    <div class="fm_22" style="font-size: 1.2em">
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                        In 2018, the team iGEM18_SCAU-China chose a strong <i>E. coli</i> endogenous promoter
 +
                        (P<I>rplJ</i>，<a href="http://parts.igem.org/Part:BBa_K2559003">BBa_K2559003</a>) to express their
 +
                        amended eGFP (<a href="http://parts.igem.org/Part:BBa_K2559005">BBa_K2559005</a>). This year our team
 +
                        JiangnanU_China also chose this promoter P<I>rplJ</i> (BBa_K2559003) to express their amended eGFP
 +
                        (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength.
 +
                        However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h
 +
                        and 10 h.(with the help of <a href="https://2019.igem.org/Measurement/Resources"> Measurement
 +
                        Hub Resources</a>，Fig 3.，Fig 4.) Through the change of value
 +
                        what we measured, we can found the promoter P<I>rplJ</i> worked well. On the other hand, we observed
 +
                        the eGFP fluorescent protein by fluorescence microscope and we found that the <i>E. coli</i> with the
 +
                        amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.
 +
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             <img src="https://static.igem.org/mediawiki/2019/1/17/T--JiangnanU_China--experiment-measurement_1.png"
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             <img src="https://static.igem.org/mediawiki/2019/thumb/c/c2/T--JiangnanU_China--new.zhang.png/1200px-T--JiangnanU_China--new.zhang.png"
 
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Latest revision as of 20:35, 21 October 2019

JiangNan

In 2018, the team iGEM18_SCAU-China chose a strong E. coli endogenous promoter (PrplJ，BBa_K2559003) to express their amended eGFP (BBa_K2559005). This year our team JiangnanU_China also chose this promoter PrplJ (BBa_K2559003) to express their amended eGFP (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength. However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h and 10 h.(with the help of Measurement Hub Resources，Fig 3.，Fig 4.) Through the change of value what we measured, we can found the promoter PrplJ worked well. On the other hand, we observed the eGFP fluorescent protein by fluorescence microscope and we found that the E. coli with the amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.
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