Difference between revisions of "Team:DTU-Denmark/Parts"
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<div class="sizemargins"> | <div class="sizemargins"> | ||
| − | <p>This is a strong promoter for Aspergillus niger that has high activity in the exponential phase.</p> | + | <p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the exponential phase.</p> |
| Line 627: | Line 627: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | Ttef (Domesticated from Aspergillus nidulans) | + | Ttef (Domesticated from <i>Aspergillus nidulans</i>) |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046018"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046018</button></a><button class="collapsiblebig">Ttef (Domesticated from Aspergillus nidulans)</button> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046018"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046018</button></a><button class="collapsiblebig">Ttef (Domesticated from <i>Aspergillus nidulans</i>)</button> |
| Line 649: | Line 649: | ||
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from Aspergillus nidulans)" style="max-width: 721px;" > | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from <i>Aspergillus nidulans</i>)" style="max-width: 721px;" > |
</p> | </p> | ||
| Line 659: | Line 659: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | Export signal from A. niger GlaA protein | + | Export signal from <i>A. niger</i> GlaA protein |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046019"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046019</button></a><button class="collapsiblebig">Export signal from A. niger GlaA protein</button> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046019"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046019</button></a><button class="collapsiblebig">Export signal from <i>A. niger</i> GlaA protein</button> |
| Line 681: | Line 681: | ||
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from A. niger GlaA protein" style="max-width: 721px;"> | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from <i>A. niger</i> GlaA protein" style="max-width: 721px;"> |
</p> | </p> | ||
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/55/T--DTU-Denmark--GlaAPcomp.jpg" alt="Compatibility of export signal from A. niger GlaA protein" style="max-width: 170px;"> </div></div> | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/55/T--DTU-Denmark--GlaAPcomp.jpg" alt="Compatibility of export signal from <i>A. niger</i> GlaA protein" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
| Line 756: | Line 756: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | PyrG selection marker from A. fumigatus | + | PyrG selection marker from <i>A. fumigatus</i> |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from A. fumigatus</button> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from <i>A. fumigatus</i></button> |
| Line 778: | Line 778: | ||
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from A. fumigatus" style="max-width: 145px;"> | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 145px;"> |
</p> | </p> | ||
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from A. fumigatus" style="max-width: 170px;"> </div></div> | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
| Line 819: | Line 819: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | AMA1 sequence for autonomous replication in Aspergillus | + | AMA1 sequence for autonomous replication in <i>Aspergillus</i> |
##############################################################################################--> | ##############################################################################################--> | ||
| Line 841: | Line 841: | ||
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in Aspergillus" style="max-width: 721px;" > | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 721px;" > |
</p> | </p> | ||
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c9/T--DTU-Denmark--AMA1.jpg" alt="Compatibility of AMA1 sequence for autonomous replication in Aspergillus" style="max-width: 170px;"> </div></div> | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c9/T--DTU-Denmark--AMA1.jpg" alt="Compatibility of AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
| Line 851: | Line 851: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | PyrG CDS from Aspergillus fumigatus | + | PyrG CDS from <i>Aspergillus fumigatus</i> |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046028"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046028</button></a><button class="collapsiblebig">PyrG CDS from Aspergillus fumigatus</button> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046028"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046028</button></a><button class="collapsiblebig">PyrG CDS from <i>Aspergillus fumigatus</i> </button> |
| Line 873: | Line 873: | ||
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from Aspergillus fumigatus" style="max-width: 721px;" | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 721px;" |
</p> | </p> | ||
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gPyrGCDScomp.jpg" alt="Compatibility of PyrG CDS from Aspergillus fumigatus" style="max-width: 170px;"> </div></div> | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gPyrGCDScomp.jpg" alt="Compatibility of PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
| Line 883: | Line 883: | ||
<!--############################################################################################# | <!--############################################################################################# | ||
| − | PyrG promoter from Aspergillus fumigatus | + | PyrG promoter from <i>Aspergillus fumigatus</i> |
##############################################################################################--> | ##############################################################################################--> | ||
<div class="buttonshadow"> | <div class="buttonshadow"> | ||
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046029"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046029</button></a><button class="collapsiblebig">PyrG promoter from Aspergillus fumigatus</button> | + | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046029"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046029</button></a><button class="collapsiblebig">PyrG promoter from <i>Aspergillus fumigatus</i> </button> |
| Line 905: | Line 905: | ||
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/0/00/T--DTU-Denmark--PyrG29_gene.jpg" alt="The figure shows the 418 basepair long PyrG promoter from Aspergillus fumigatus" style="max-width: 721px;"> | + | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/0/00/T--DTU-Denmark--PyrG29_gene.jpg" alt="The figure shows the 418 basepair long PyrG promoter from <i>Aspergillus fumigatus</i>" style="max-width: 721px;"> |
</p> | </p> | ||
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/03/T--DTU-Denmark--PyrG29.jpg" alt="Compatibility of PyrG promoter from Aspergillus fumigatus" style="max-width: 170px;"> </div></div> | + | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/03/T--DTU-Denmark--PyrG29.jpg" alt="Compatibility of PyrG promoter from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div> |
</div> | </div> | ||
Revision as of 20:15, 21 October 2019
Parts overview
We made parts
Part Table
Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.
Type: Regulatory
Size: 683 bp
This is a strong promoter for Aspergillus niger that has high activity in the exponential phase.
Assembly Compatibility:
Type: Regulatory
Size: 842 bp
This is a medium strength constitutive promoter for Aspergillus niger.
Assembly Compatibility:
Type: Regulatory
Size: 926 bp
This is a strong constitutive promoter for Aspergillus niger that has especially high activity in the lag phase.
Assembly Compatibility:
Type: Regulatory
Size: 926 bp
This is a medium strength constitutive promoter for Aspergillus niger.
Assembly Compatibility:
Type: Regulatory
Size: 986 bp
This is a very strong constitutive promoter for Aspergillus niger that has especially high activity in the exponential growth phase.
Assembly Compatibility:
Type: Regulatory
Size: 933 bp
This is a weak constitutive promoter for Aspergillus niger.
Assembly Compatibility:
Type: Regulatory
Size: 973 bp
This is a strong promoter for Aspergillus niger that has high activity in the late exponential phase and stationary phase.
Assembly Compatibility:
Type: Regulatory
Size: 1014 bp
This is a strong promoter for Aspergillus niger that has high activity in the stationary growth phase.
Assembly Compatibility:
Type: Composite
Size: 1324 bp
This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.
Assembly Compatibility:
Type: Plasmid Backbone
Size: 7896 bp
This is a plasmid containing an E.coli ori, an ampicillin selection marker, and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.
Assembly Compatibility:
