Difference between revisions of "Team:JiangnanU China/Improve"

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<div class="column full_size judges-will-not-evaluate">
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        #HQ_page h1, h2, h3, h4, h5 {
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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        @font-face {
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<h1>Improve a Previous Part or Previous Project</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part's main page on the Registry. Please include a link to your improved part's Registry page on this page.</p>
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            src: url('https://static.igem.org/mediawiki/2019/4/49/T--JiangnanU_China--FuturaFuturisC-Italic.otf');
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<h3>Gold Medal Criterion #2</h3>
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        @font-face {
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<p><b>Improve a Previous Part - Standard Tracks:</b> Convince the judges that you have created a new BioBrick Part that has a functional improvement upon an existing BioBrick Part. You must perform experiments with both parts to demonstrate this improvement. Clearly document the quantitative experimental characterization data on the Part's Main Page on the Registry for both the existing and new parts (see the <a href="http://parts.igem.org/Help:Document_Parts">Registry Document Parts page</a> for instructions).
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<br><br>
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            src: url('https://static.igem.org/mediawiki/2019/1/11/T--JiangnanU_China--Futura-Medium-6.otf');
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Both the existing and new part must be <a href="http://parts.igem.org/Help:Standards/Assembly/RFC10">RFC10</a> or Type IIS compatible. The sequences of the new and existing parts must be different. The existing part must NOT be from your 2019 part number range and must be different from the part you used in Bronze #4. The new part you create must also be different from the new part documented in Silver #1. Please see the <a href="https://2019.igem.org/Measurement/Resources">Measurement Resources page</a> for more information about experimental characterization data.  
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<br><br>
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<b>Improve a Previous Project - Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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                    <b>Improvement</b>
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                </div>
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                <br/><br/><br/><br/>
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                <img src="https://static.igem.org/mediawiki/2019/9/95/T--JiangnanU_China--improvement_0.png"
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                    This year, we improved the sequence of the RBS <a href="http://parts.igem.org/wiki/index.php/Part:BBa_B0033" style="color:blue;"alt="">BBa_B0033</a>,
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                    and got the RBS <a href="http://parts.igem.org/Part:BBa_K3137001" style="color:blue;"alt="">BBa_K3137001</a> .
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<img src="https://static.igem.org/mediawiki/2019/2/22/T--JiangnanU_China--imp.png"
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                As we all know, RBS refers to a sputum-rich untranslated region upstream of the initiation codon AUG.
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                BBa_B0033 is a weaker RBS based on Ron Weiss thesis. Compared with BBa_B0030, BBa_B0031, BBa_B0032,
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                BBa_B0034, B0033 is weakest, and whose RBS strength is 0.35% of B0034. By analyzing their sequence, we
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                found that B0033 has fewer purines and no not have AAA sequence. SO we designed the RBS by adjusting the
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                proportion of bases.
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            <img src="https://static.igem.org/mediawiki/2019/6/6c/T--JiangnanU_China--improvement_1.png"
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                Then we ligated the two RBS with promotor P<I>rsmH</i> (BBa_K3137000) and gene <I>gfp</i> (BBa_K3137011 ) and transform
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                into <i>E. coli</i> BL21 to compare their green fluorescence brightness.
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                The figure below shows that BBa_K3137001 has the stronger fluorescently protein expression than
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                BBa_B0033, that means it’s efficient to increase the protein expression by increasing the number of
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                purines in the RBS sequence, which is beneficial to the binding of ribosomes.
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                <img src="https://static.igem.org/mediawiki/2019/1/1b/T--JiangnanU_China--improvement_2.png"
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                <img src="https://static.igem.org/mediawiki/2019/2/21/T--JiangnanU_China--improved2.png"
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{{:Team:JiangnanU_China/Footer}}

Latest revision as of 20:08, 21 October 2019

JiangNan

This year, we improved the sequence of the RBS BBa_B0033, and got the RBS BBa_K3137001 .
As we all know, RBS refers to a sputum-rich untranslated region upstream of the initiation codon AUG. BBa_B0033 is a weaker RBS based on Ron Weiss thesis. Compared with BBa_B0030, BBa_B0031, BBa_B0032, BBa_B0034, B0033 is weakest, and whose RBS strength is 0.35% of B0034. By analyzing their sequence, we found that B0033 has fewer purines and no not have AAA sequence. SO we designed the RBS by adjusting the proportion of bases.
Then we ligated the two RBS with promotor PrsmH (BBa_K3137000) and gene gfp (BBa_K3137011 ) and transform into E. coli BL21 to compare their green fluorescence brightness. The figure below shows that BBa_K3137001 has the stronger fluorescently protein expression than BBa_B0033, that means it’s efficient to increase the protein expression by increasing the number of purines in the RBS sequence, which is beneficial to the binding of ribosomes.
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