Difference between revisions of "Team:JiangnanU China/Measurement"

In 2018, the team iGEM18_SCAU-China chose a strong E. coli endogenous promoter (PrplJ，BBa_K2559003) to express their amended eGFP (BBa_K2559005). This year our team JiangnanU_China also chose this promoter PrplJ (BBa_K2559003) to express their amended eGFP (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength. However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h and 10 h.(with the help of Measurement Hub Resources，Fig 3.，Fig 4.) Through the change of value what we measured, we can found the promoter PrplJ worked well. In the other hand, we observed the eGFP fluorescent protein by fluorescence microscope and we found that the E. coli with the amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.