Difference between revisions of "Team:DTU-Denmark/Parts"

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<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project</p>
+
<p>This is a strong promoter for Aspergillus niger that has high activity in the exponential phase.</p>
  
  
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1a/T--DTU-Denmark--glaA.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_2" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1a/T--DTU-Denmark--glaA.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_2" style="max-width: 721px;"   
 
</p>
 
</p>
  
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<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project</p>
+
<p>This is a medium strength constitutive promoter for <i>Aspergillus niger</i>.</p>
  
  
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/8/88/T--DTU-Denmark--sonB.jpg" alt="The figure shows 842 basepair long promoter PLEAPsonB_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/8/88/T--DTU-Denmark--sonB.jpg" alt="The figure shows 842 basepair long promoter PLEAPsonB_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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<div class="sizemargins">
 
<div class="sizemargins">
−
<p>This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project</p>
+
<p>This is a strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the lag phase.
 +
 
 +
</p>
  
  
−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--gpdA.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--gpdA.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/3/3d/T--DTU-Denmark--gdpA2.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_2" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/3/3d/T--DTU-Denmark--gdpA2.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_2" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e8/T--DTU-Denmark--mstA.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e8/T--DTU-Denmark--mstA.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/6f/T--DTU-Denmark--unk1.jpg" alt="The figure shows the 933 basepair long promoter PLEAPunk_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/6f/T--DTU-Denmark--unk1.jpg" alt="The figure shows the 933 basepair long promoter PLEAPunk_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--gfaA1.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--gfaA1.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/93/T--DTU-Denmark--hfbD.jpg" alt="The figure shows the 1014 basepair long promoter PLEAPhfbD_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/93/T--DTU-Denmark--hfbD.jpg" alt="The figure shows the 1014 basepair long promoter PLEAPhfbD_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/2/23/T--DTU-Denmark--glaA1.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/2/23/T--DTU-Denmark--glaA1.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/7/7f/T--DTU-Denmark--rsdA_1.jpg" alt="The figure shows the 605 basepair long promoter PLEAPrsdA_1" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/7/7f/T--DTU-Denmark--rsdA_1.jpg" alt="The figure shows the 605 basepair long promoter PLEAPrsdA_1" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/14/T--DTU-Denmark--sonB_2.jpg" alt="The figure shows the 842 basepair long promoter PLEAPsonB_2" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/14/T--DTU-Denmark--sonB_2.jpg" alt="The figure shows the 842 basepair long promoter PLEAPsonB_2" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--mstA21.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_2" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--mstA21.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_2" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5d/T--DTU-Denmark--unk4.jpg" alt="The figure shows the 724 basepair long promoter PLEAPunk_4" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5d/T--DTU-Denmark--unk4.jpg" alt="The figure shows the 724 basepair long promoter PLEAPunk_4" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gfaA_2.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_2" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gfaA_2.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_2" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/fd/T--DTU-Denmark--unk6.jpg" alt="The figure shows the 973 basepair long promoter PLEAPunk_6" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/fd/T--DTU-Denmark--unk6.jpg" alt="The figure shows the 973 basepair long promoter PLEAPunk_6" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/59/T--DTU-Denmark--moclo.jpg" alt="The figure shows the 238 basepair long MoClo promoter standi-n" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/59/T--DTU-Denmark--moclo.jpg" alt="The figure shows the 238 basepair long MoClo promoter standi-n" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from Aspergillus nidulans)" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from Aspergillus nidulans)" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from A. niger GlaA protein" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from A. niger GlaA protein" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/67/T--DTU-Denmark--moxgfp.jpg" alt="The figure shows the 720 basepair long MoxGFP" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/67/T--DTU-Denmark--moxgfp.jpg" alt="The figure shows the 720 basepair long MoxGFP" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in Aspergillus" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in Aspergillus" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from Aspergillus fumigatus" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from Aspergillus fumigatus" style="max-width: 721px;"   
 
</p>
 
</p>
  
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−
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/d/de/T--DTU-Denmark--IS2dw.jpg" alt="The figure shows the 2112 basepair IS2 downstream sequence for A.niger genome integration" style="max-width: 100%;"   
+
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/d/de/T--DTU-Denmark--IS2dw.jpg" alt="The figure shows the 2112 basepair IS2 downstream sequence for A.niger genome integration" style="max-width: 721px;"   
 
</p>
 
</p>
  

Revision as of 17:38, 21 October 2019

Parts overview

We made parts

Part Table

Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.

Type: Regulatory

Size: 683 bp

This is a strong promoter for Aspergillus niger that has high activity in the exponential phase.

The figure shows the 683 basepair long promoter PLEAPglaA_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPglaA_2

Type: Regulatory

Size: 842 bp

This is a medium strength constitutive promoter for Aspergillus niger.

The figure shows 842 basepair long promoter PLEAPsonB_1

Assembly Compatibility:

The figure shows the compatibility of promoter PLEAPsonB_1

Type: Regulatory

Size: 926 bp

This is a strong constitutive promoter for Aspergillus niger that has especially high activity in the lag phase.

The figure shows the 926 basepair long promoter PLEAPgdpA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPgpdA_1

Type: Regulatory

Size: 926 bp

Coming soon

The figure shows the 926 basepair long promoter PLEAPgdpA_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPgpdA_2

Type: Regulatory

Size: 986 bp

Coming soon

The figure shows the 986 basepair long promoter PLEAPmstA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPmstA_1

Type: Regulatory

Size: 933 bp

Coming soon

The figure shows the 933 basepair long promoter PLEAPunk_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPunk_1

Type: Regulatory

Size: 973 bp

Coming soon

The figure shows the 973 basepair long promoter PLEAPgfaA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPgfaA_1

Type: Regulatory

Size: 1014 bp

Coming soon

The figure shows the 1014 basepair long promoter PLEAPhfbD_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPhfbD_1

Type: Measurement

Size: 1505 bp

Coming soon

The figure shows the components of the fungal MoClo promoter test device

Assembly Compatibility:

The figure shows the compatibility of the fungal MoClo promoter test device

Type: Regulatory

Size: 683 bp

Coming soon

The figure shows the 683 basepair long promoter PLEAPglaA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPglaA_1

Type: Regulatory

Size: 605 bp

Coming soon

The figure shows the 605 basepair long promoter PLEAPrsdA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPrsdA_1

Type: Regulatory

Size: 842 bp

Coming soon

The figure shows the 842 basepair long promoter PLEAPsonB_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPsonB_2

Type: Regulatory

Size: 986 bp

Coming soon

The figure shows the 986 basepair long promoter PLEAPmstA_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPmstA_2

Type: Regulatory

Size: 724 bp

Coming soon

The figure shows the 724 basepair long promoter PLEAPunk_4

Assembly Compatibility:

The figure shows the compatibility of PLEAPunk_4

Type: Regulatory

Size: 973 bp

Coming soon

The figure shows the 973 basepair long promoter PLEAPgfaA_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPgfaA_2

Type: Regulatory

Size: 480 bp

Coming soon

The figure shows the 973 basepair long promoter PLEAPunk_6

Assembly Compatibility:

The figure shows the compatibility of PLEAPunk_6

Type: Regulatory

Size: 238 bp

Coming soon

The figure shows the 238 basepair long MoClo promoter standi-n

Assembly Compatibility:

The figure shows the compatibility of the MoClo promoter stand-in

Type: Terminator

Size: 485 bp

Coming soon

The figure shows the 485 basepair long Ttef (Domesticated from Aspergillus nidulans)

Assembly Compatibility:

The figure shows the compatibility of Ttef

Type: Tag

Size: 63 bp

Coming soon

The figure shows the 63 basepair long Export signal from A. niger GlaA protein

Assembly Compatibility:

Compatibility of export signal from A. niger GlaA protein

Type: Coding

Size: 720 bp

Coming soon

The figure shows the 720 basepair long MoxGFP

Assembly Compatibility:

Compatibility of MoxGFP

Type: Measurement

Size: 10725 bp

Coming soon

The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr

Assembly Compatibility:

Compatibility of promoter test plasmid pAMA1-zing_Pyr

Type: Composite

Size: 1324 bp

Coming soon

The figure shows the 1324 basepair long PyrG selection marker from A. fumigatus <div class=

Assembly Compatibility:

Compatibility of PyrG selection marker from A. fumigatus

Type: Plasmid Backbone

Size: 7896 bp

Coming soon

The figure shows the 7896 basepair long pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone

Assembly Compatibility:

Compatibility of pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone

Type: Coding

Size: 5721 bp

Coming soon

The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in Aspergillus

Assembly Compatibility:

Compatibility of AMA1 sequence for autonomous replication in Aspergillus

Type: Coding

Size: 906 bp

Coming soon

The figure shows the 906 basepair long PyrG CDS from Aspergillus fumigatus

Assembly Compatibility:

Compatibility of PyrG CDS from Aspergillus fumigatus

Type: Regulatory

Size: 418 bp

Coming soon

The figure shows the 418 basepair long PyrG promoter from Aspergillus fumigatus

Assembly Compatibility:

Compatibility of PyrG promoter from Aspergillus fumigatus

Type: Composite

Size: 5546 bp

Coming soon

The figure shows the 5546 basepair long pGIA2P1 insert

Assembly Compatibility:

Compatibility of pGIA2P1 insert

Type: Measurement

Size: 7139 bp

Coming soon

The figure shows the 7139 basepair long pPEA2P1 insert

Assembly Compatibility:

Compatibility of pPEA2P1 insert

Type: DNA

Size: 2096 bp

Coming soon

The figure shows the 2096 basepair long IS2 upstream sequence for A.niger genome integration

Assembly Compatibility:

Compatibility of IS2 upstream sequence for A.niger genome integration

Type: DNA

Size: 2112 bp

Coming soon

The figure shows the 2112 basepair IS2 downstream sequence for A.niger genome integration

Assembly Compatibility:

Compatibility of IS2 downstream sequence for A.niger genome integration
<groupparts>iGEM19 DTU-Denmark</groupparts>
The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience