Difference between revisions of "Team:HK SSC/Improve"
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<p> For part improvement, we decided to improve part <a href="http://parts.igem.org/Part:BBa_K1689013">BBa_K1689013</a> | <p> For part improvement, we decided to improve part <a href="http://parts.igem.org/Part:BBa_K1689013">BBa_K1689013</a> | ||
| − | + | <h3>Original Part BBa_K1689013</h3> | |
| + | <p>Part BBa_K1689013 is an N-terminal fragment of β-lactamase fused with dCas9. Team iGEM15_Peking designed it to be resistance against several antibiotics. However, β-lactamase may not be applicable to each and every project. For example, in our project, the plasmid already confers Kanamycin resistance gene. β-lactamase may not be applicable in this situation. Other than using β-lactamase as a selection method, we hope to provide more options for CRISPR imaging. The GFP will allow visual confirmation of successful transformation and indicates that the dCas9 enzyme has been successfully expressed.</p> | ||
</html> | </html> | ||
Revision as of 14:17, 21 October 2019
Improve a Previous Part or Previous Project
For part improvement, we decided to improve part BBa_K1689013
Original Part BBa_K1689013
Part BBa_K1689013 is an N-terminal fragment of β-lactamase fused with dCas9. Team iGEM15_Peking designed it to be resistance against several antibiotics. However, β-lactamase may not be applicable to each and every project. For example, in our project, the plasmid already confers Kanamycin resistance gene. β-lactamase may not be applicable in this situation. Other than using β-lactamase as a selection method, we hope to provide more options for CRISPR imaging. The GFP will allow visual confirmation of successful transformation and indicates that the dCas9 enzyme has been successfully expressed.