Difference between revisions of "Team:HK SSC/Design"
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Revision as of 08:41, 21 October 2019
Design
Expression of dCas9-sgRNA-GFP Complex for gene silencing
We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB.
Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA.