Difference between revisions of "Team:HK SSC/Design"
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<h1 style="color: purple; font-size: 25px;">Design</h1> | <h1 style="color: purple; font-size: 25px;">Design</h1> | ||
<h1><u>Expression of dCas9-sgRNA-GFP Complex for gene silencing</u></h1> | <h1><u>Expression of dCas9-sgRNA-GFP Complex for gene silencing</u></h1> | ||
| − | <p>We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB. | + | <p>We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB.</p> |
</br> | </br> | ||
| − | Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA. </p> | + | <p>Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA. </p> |
</html> | </html> | ||
Revision as of 08:34, 21 October 2019
Design
Expression of dCas9-sgRNA-GFP Complex for gene silencing
We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB.
Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA.