Difference between revisions of "Team:DTU-Denmark/Integrated Human Practices"

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<p>There are different types of wastewater treatment plants, and we had the opportunity to both meet municipal as well as private plants. Process engineer <b>Peter Gruvstedt from the private plant at AstraZeneca</b> thought that Biotic Blue could be implemented in their fungal process step of purification, if an additional system was built on the side of the compartment, and if the enzyme was optimized for their pharmaceuticals. AstraZeneca’s treatment plant was especially interesting for Biotic Blue since the pH of the fungal treatment is optimal for the enzyme as well as a controlled temperature.</p>
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        <h5><i>"This could reduce the amount of sulfamethoxazole in both WWTP effluents, the wastewater and the sludge."</i> - Pharem Biotech</h5>
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        <p>Meeting with the CEO <b>Martin Ryen</b> and <b>Domenico Palumberi</b> from Pharem, we had the opportunity to pitch our idea - Biotic Blue. Pharem produces enzymes for treating Swedish wastewater, and they both gave us useful advice and insights in the lab procedure. Martin, the founder and CEO of Pharem thought that it would be feasible to incorporate Biotic Blue before the biological treatment of wastewater since it would reduce the risk of triggering antibiotic resistance. After our meeting, we got back to revise our product implementation and discuss in which location our vision makes the biggest impact. </p>
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Revision as of 22:53, 20 October 2019

Integrated Human Practices

A huge goal for our project has been to secure that it has the biggest impact possible. This has meant that we have been in contact with important stakeholders and their advice has shaped our approach and the path of the project.

Being the number one group of organism to industrially produce enzymes, filamentous fungi are exceptionally important to the everyday lives of many people even though they might not notice it.
LEAP started as a reaction to the extreme lack of resources to work with filamentous fungi and attempted to use synthetic biology to contribute to the list of tools necessary to work with these organisms.
Originally, the project aimed to make a promoter library for either filamentous fungi, yeast, moss, or maybe all three, but following discussions with several companies and scientists, the team decided to develop a software that enabled the creation of promoters that could be applied to any organism and tested the function of the software in Aspergilli.

Our stakeholder analysis led us to talk to 3 different companies: Zymergen, Novozymes, and Bolt Threads. We also talked to 3 fungal experts: Peter Richard (VTT, Finland), Jens Christian Frisvad (DTU, Denmark) and Jakob Blæsbjerg (DTU, Denmark). Additionally, we addressed the public by attending Science EXPO in Copenhagen, two biotechnology camps for high school students, and teaching synthetic biology in two high schools.
Fig. 1: Overview of the process for integrated human practices throughout the project.
The stakeholder analysis shows a square with four sections, which divides stakeholder’s into four categories: high interest and high power, high interest and low power, low interest, and high power, and low interest and low power.
Fig. 2: Stakeholder analysis

Stakeholder Analysis

In March, the team looked into the impacts of the project on different stakeholders and therefore made a stakeholder analysis, as shown in figure 2. This stakeholder analysis reveals that companies such as Novozymes, Zymergen, and Bolt Threads are among the most important to our project, both in interest and power. This means that their opinions should be managed closely. Additionally, researchers such as Jens Frisvad (DTU, Denmark), Jakob Blæsbjerg (DTU, Denmark), and Peter Richard (VTT, Finland) could benefit from our project, making them important stakeholders. Although other iGEM teams do not have a lot of power, their interest could nevertheless be great and they should, therefore, be well informed. The public and the DTU BlueDot program will most likely not take much interest in the project as a promoter library can be a very technical concept and not immediately usable by non-specialists. However, as DTU BlueDot is a big sponsor of the team, they are important to keep satisfied.

Based on the stakeholder analysis, we decided to contact three different biotech companies; Novozymes, Zymergen, and Bolt Threads as all three companies work with genetically modified filamentous fungi. We asked them how our project could influence their work and for suggestions regarding the experiment.

We also reached out to several scientists, including Jakob Blæsbjerg from DTU and Peter Richard from VTT (Technical Research Centre) in Finland. They helped provide us with protocols and advice on how to grow the fungi and how to ensure reproducible and comparable results.

Even though the public is neither very powerful nor interested in the project according to our stakeholder analysis, we nevertheless decided to contact high schools in order to talk to young people about synthetic biology and its aspects as well as about our project and what good it can do in the world. We also partook in several events: The annual UNF (Ungdommens Naturvidenskabelige Forening) Biotech Camp; Science Expo, a large science fair in Copenhagen; and the annual Biotech Academy Camp in order to increase their knowledge and interest in a topic like synthetic biology. This is described further on Education and Engagement.

The stakeholder analysis shows a square with four sections, which divides stakeholder’s into four categories: high interest and high power, high interest and low power, low interest and high power, and low interest and low power.
Fig. 2: Stakeholder analysis
Zymergen Zymergen Peter Richards Peter Richards Novozymes Novozymes Bolt Threads Bolt Threads Jens Frisvad Jens Frisvad Jakob Blæsbjerg Jakob Blæsbjerg



The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience