Difference between revisions of "Team:JiangnanU China/Results"

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{{JiangnanU_China}}
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        #HQ_page h1, h2, h3, h4, h5 {
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h3>What should this page contain?</h3>
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<ul>
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        @font-face {
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<li> Clearly and objectively describe the results of your work.</li>
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            font-family: 'Futura_Bold';
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<li> Future plans for the project. </li>
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            src: url('https://static.igem.org/mediawiki/2019/e/e0/T--JiangnanU_China--ziti_Futura_Bold.otf');
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<li> Considerations for replicating the experiments. </li>
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</div>
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            font-family: 'FuturaFuturisC-Italic';
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            src: url('https://static.igem.org/mediawiki/2019/4/49/T--JiangnanU_China--FuturaFuturisC-Italic.otf');
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            font-family: 'Futura-Medium-6';
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            src: url('https://static.igem.org/mediawiki/2019/1/11/T--JiangnanU_China--Futura-Medium-6.otf');
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        }
  
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        .fb_22 {
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<h3>Describe what your results mean </h3>
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            font-family: Futura_Bold, sans-serif;
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            font-size: 1.3em;
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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        }
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<li> Show data, but remember <b>all measurement and characterization data must also be on the part's Main Page on the Registry.</b> Otherwise these data will not be in consideration for any medals or part awards! </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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</ul>
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<h3> Project Achievements </h3>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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        /*无书签正文*/
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<li>A list of linked bullet points of the successful results during your project</li>
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        .row {
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h3>Inspiration</h3>
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            background-image: url('https://static.igem.org/mediawiki/2019/b/bc/T--JiangnanU_China--results_bgd.png');
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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            width: 100%;
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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                    <b>Results</b>
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                <br/>
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                    In order to make <i>E. coli</i> in the laboratory resistant to phage infection, this year our team
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                    first
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                    found components that responded to phage infection through transcriptome analysis.
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                    Then we found components that could make the bacteria resistant to phage infection through
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                    literature search and mutagenesis screening.
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                Phage Isolation
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                We added 1 μL of phage-infected fermentation broth to a plate containing <i>E. coli</i> BL21.
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                <br/>
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                After proper culture for a period of time, plaque appeared on the plate (Fig.1.)
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                <br/>
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                We isolated the phages from the plate and photographed them using a projective electron microscope (Fig.
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                2).
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                <br/>
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                We finally determined that the T4 phages infected our fermentation broth by sequencing the genome of the
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                phages.
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            <!--第二部分-->
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            <div class="fb_72"><b>Selection of Inducible Promoters</b></div>
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                1.Selection
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                In order to screen inducible promoters, we first made the one-step growth curve of phages (Fig. 3).
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                After that, we selected two time points of phage infection for 5min （in the incubation period of phage
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                infection）and phage infection for 20min （in the outbreak period of phage infection）through the one-step
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                growth curve of phage.
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                By analyzing transcriptome data，we selected inducible promoter P<i>putA</i> (Fig.4) for 5 min and
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                inducible
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                promoter P<i>glcF</i> (Fig.5) for 20 min.
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                2. Characterization
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                In <i>E. coli</i> BL21，we connected the green fluorescence gene <i>gfp</i> with the inducible promoter P<i>putA</i>
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                for 5 min
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                (Fig.6) and the red fluorescence gene <i>mCherry</i> with the inducible promoter P<i>glcF</i> for 20 min
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                (Fig.7) in
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                our genetic circuits.
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                After infecting the bacteria with phages for the corresponding time, we observed that the infected cells
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                gave off green and red fluorescence respectively.
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{{:Team:JiangnanU_China/Footer}}

Revision as of 08:05, 20 October 2019

JiangNan

Phage Isolation
We added 1 μL of phage-infected fermentation broth to a plate containing E. coli BL21.
After proper culture for a period of time, plaque appeared on the plate (Fig.1.)
We isolated the phages from the plate and photographed them using a projective electron microscope (Fig. 2).
We finally determined that the T4 phages infected our fermentation broth by sequencing the genome of the phages.
Selection of Inducible Promoters
1.Selection
In order to screen inducible promoters, we first made the one-step growth curve of phages (Fig. 3). After that, we selected two time points of phage infection for 5min （in the incubation period of phage infection）and phage infection for 20min （in the outbreak period of phage infection）through the one-step growth curve of phage. By analyzing transcriptome data，we selected inducible promoter PputA (Fig.4) for 5 min and inducible promoter PglcF (Fig.5) for 20 min.
2. Characterization
In E. coli BL21，we connected the green fluorescence gene gfp with the inducible promoter PputA for 5 min (Fig.6) and the red fluorescence gene mCherry with the inducible promoter PglcF for 20 min (Fig.7) in our genetic circuits. After infecting the bacteria with phages for the corresponding time, we observed that the infected cells gave off green and red fluorescence respectively.
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