Difference between revisions of "Team:DTU-Denmark/Safety"
LouiseAlmer (Talk | contribs) |
|||
| (7 intermediate revisions by one other user not shown) | |||
| Line 17: | Line 17: | ||
<section id="top" class="header_con "> | <section id="top" class="header_con "> | ||
<div class="container"> | <div class="container"> | ||
| − | <div class="row flex-center " style="padding-top:75px;"> | + | <div class="row flex-center sm-no-flex" style="padding-top:75px;"> |
| Line 69: | Line 69: | ||
<div class="row flex-center interlabspace"> | <div class="row flex-center interlabspace"> | ||
| − | <div class=" sm-no-float col-md-4 bbmobile col-sm- | + | <div class=" sm-no-float col-md-4 bbmobile col-sm-11"> |
<img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--safetysvg1.svg" alt="" class="safetyfirstimg"/> | <img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--safetysvg1.svg" alt="" class="safetyfirstimg"/> | ||
| Line 87: | Line 87: | ||
</div> | </div> | ||
| − | + | </div> | |
| − | <div class=" sm-no-float col-md- | + | <div class="row flex-center bbmobileshow"> |
| + | <div class=" sm-no-float col-md-12 col-sm-12 "> | ||
<img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--safetysvg1.svg" alt="" class="safetyfirstimg"/> | <img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--safetysvg1.svg" alt="" class="safetyfirstimg"/> | ||
| Line 98: | Line 99: | ||
| − | <div class="sm-no-float col-md-8 | + | <div class="sm-no-float col-md-8" > |
| Line 115: | Line 116: | ||
| − | <div class=" sm-no-float col-md-4 col-sm-12"> | + | <div class=" sm-no-float col-md-4 col-sm-12" style="margin-right:25px"> |
<img src="https://static.igem.org/mediawiki/2019/6/64/T--DTU-Denmark--safetysvg2.svg" alt="" class="safetysecondimg"/> | <img src="https://static.igem.org/mediawiki/2019/6/64/T--DTU-Denmark--safetysvg2.svg" alt="" class="safetysecondimg"/> | ||
| Line 194: | Line 195: | ||
</footer> | </footer> | ||
| − | <img src="https://static.igem.org/mediawiki/2019/9/91/T--DTU-Denmark--biggrants.svg" title="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden" | + | <img class="footergrants" src="https://static.igem.org/mediawiki/2019/9/91/T--DTU-Denmark--biggrants.svg" title="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden" alt="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden"> |
| − | + | <img class="footersponsors" src="https://static.igem.org/mediawiki/2019/d/d9/T--DTU-Denmark--sponsorsfooter.svg" title="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience" alt="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience"></div></div> | |
| − | <img src="https://static.igem.org/mediawiki/2019/d/d9/T--DTU-Denmark--sponsorsfooter.svg" title="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience" | + | |
| − | + | ||
| − | </div></div> | + | |
</html> | </html> | ||
Latest revision as of 16:54, 19 October 2019
Safety
Regarding the organism
Filamentous fungi form the base of our project, and as mentioned in the project description, they are important producers in the biotechnological industry.
We have chosen to work with Aspergillus niger as it is an important production organism, and the tools required to genetically engineer the organism already exist. The specific strain we are working with, ATCC1015 [1], is classified as Biosafety Level 1, indicating that it is safe to use and handle in the lab. In addition to this declaration, we have performed a safety check-in, as spore-forming fungi are not directly on the whitelist.
Aspergillus spores are airborne and cannot only contaminate other samples but additionally cause aspergillosis. Therefore, anytime we worked with Aspergillus we worked in a biosafety cabinet which was cleaned thoroughly with ethanol before and after work.
Fermentation safety
During the project, we will be using bioreactors to verify our promoters. This introduces new challenges in safely handling our organism and the larger equipment. The production of spores by Aspergillus niger and many other filamentous fungi is dependent on external conditions of the fermentation. That is why, during our work with fermentation, only members who had received training in bioreactors participated in the experiment. These team members wore safety goggles, gloves, and lab coats whenever entering the fermentation platform and made sure that qualified personnel were on-site for help and advice if needed.
