Difference between revisions of "Team:JiangnanU China/Parts"

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{{JiangnanU_China}}
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{{:Team:JiangnanU_China/Header}}
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<html>
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<html xmlns="http://www.w3.org/1999/html">
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<head>
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    <meta name="viewport" content="width=device-width, initial-scale=1">
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    <meta charset="utf-8"/>
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<div class="column full_size">
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        #HQ_page h1, h2, h3, h4, h5 {
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<h1>Parts</h1>
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            margin: 0;
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. The iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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        }
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</div>
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<div class="column full_size">
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        #HQ_page p {
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<div class="highlight decoration_background">
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<h3>Note</h3>
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            font-family: inherit;
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<p>Note that parts must be well documented on each part's <a href="http://parts.igem.org/Main_Page">Main Page on the Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
+
        }
−
This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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        /*字体*/
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        @font-face {
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            font-family: 'Futura_Bold';
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            src: url('https://static.igem.org/mediawiki/2019/e/e0/T--JiangnanU_China--ziti_Futura_Bold.otf');
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        }
  
 +
        @font-face {
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            font-family: 'FuturaFuturisC-Italic';
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            src: url('https://static.igem.org/mediawiki/2019/4/49/T--JiangnanU_China--FuturaFuturisC-Italic.otf');
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        }
  
 +
        @font-face {
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            font-family: 'Futura-Medium-6';
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            src: url('https://static.igem.org/mediawiki/2019/1/11/T--JiangnanU_China--Futura-Medium-6.otf');
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        }
  
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        .fb_48 {
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<h3>Adding parts to the registry</h3>
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        .fb_72 {
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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            font-family: Futura_Bold, sans-serif;
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Documentation includes the characterization data of your parts.</p>
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        .fm_22 {
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<div class="button_link">
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            font-family: Futura-Medium-6, sans-serif;
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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        }
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</a>
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</div>
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<h3>Inspiration</h3>
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            display: flex;
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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            flex-direction: row;
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        }
  
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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        /*一列*/
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<ul>
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        .column {
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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            display: flex;
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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            flex-direction: column;
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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        }
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</ul>
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</div>
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        .contents {
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            margin: 0% 15% 15%;
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        }
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        .centers {
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    <style>
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        .bgd {
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            background-image: url('https://static.igem.org/mediawiki/2019/5/59/T--JiangnanU_China--parts_0.png');
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            background-size: 100% 100%;
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            background-position: center;
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        <div class="column">
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            <div class="centers">
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                <div class="fb_72">
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                    <b>Part</b>
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                </div>
 +
                <div style="height: 60vh"></div>
 +
                <!--                View more-->
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                <a href="#phage" style="text-decoration: none">
 +
                    <div class="row" style="align-content: center;color: white;">
 +
                        <img
 +
                                src="https://static.igem.org/mediawiki/2019/0/09/T--JiangnanU_China--host_liubianxing.png"
 +
                                alt="back" style="width: 6%;height:auto;">
 +
                        <div class="fb_48" style="margin-left: 2%;margin-top: 1%">View all</div>
 +
                    </div>
 +
                </a>
 +
 
 +
            </div>
 +
        </div>
 +
    </div>
 
</div>
 
</div>
  
 +
<!--第一部分-->
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<div class="split"></div>
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<div class="contents" id="phage">
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    <div class="column">
 +
        <div class="centers">
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            <div class="fb_72">
 +
                <b>Basic Part</b>
 +
            </div>
 +
            <!--            BBa_K3137000-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
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                <b>BBa_K3137000</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>rsmH</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp50bp constitutive promoter<br/>
 +
                <i>RsmH</i> can detect the intensity of the fluorescence of <i>gfp</i>.
 +
            </div>
 +
            <!--            BBa_K3137002-->
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            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137002</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>PputA</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 50bp inducible promoter<br/>
 +
                <i>PputA</i> is the inducible promoter that When <i>E.coli</i> is infected by a phage, both report and
 +
                respond gene
 +
                circuits are induced to express at the same time. When a phage infects Escherichia coli into the
 +
                incubation period (about 5 min), <i>PputA</i> will induce the bacteria to express resistant protein and
 +
                display
 +
                green fluorescence.
 +
            </div>
  
−
<div class="clear extra_space"></div>
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            <div class="split_small"></div>
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 +
                <b>BBa_K3137003</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>PglcF </i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 50bp inducible promoter<br/>
 +
                <i>PglcF</i> is the inducible promoter that works when phages infect bacterial for around 20 min. If the
 +
                resistant proteins successfully resist phage infection, the inducible promoter <i>PglcF</i> will not
 +
                induce the
 +
                expression of downstream gene. If the resistant proteins cannot kill phages, the phages will continue to
 +
                infect. When phages infect bacteria into the outbreak period (about 20 min), the inducible promoter
 +
                <i>PglcF</i> will induce expression of the red fluorescent protein gene <i>mCherry</i> and the
 +
                downstream toxic
 +
                protein gene <i>protegrin-1</i>, which will make the bacteria display red fluorescence and lyse cells
 +
                before
 +
                the assembly of phages.
 +
            </div>
  
 +
            <!--            BBa_K3137004-->
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            <div class="split_small"></div>
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            <div class="fb_48">
 +
                <b>BBa_K3137004</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>mCherry</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 708bp marker gene<br/>
 +
                The <i>mCherry</i> can express red fluorescent protein and it is used as a marker gene in the process of
 +
                reporting the number of cells.
 +
            </div>
  
 +
            <!--            BBa_K3137005-->
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            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137005</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>gntR</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 996bp<br/>
 +
                The Gluconate repressor <i>gntR</i>, is a transcription factor that negatively regulates the operon
 +
                involved in
 +
                the catabolism of d-gluconate via the <i>Entner-Doudoroff</i> pathway and represses genes involved in
 +
                the
 +
                different systems related to d-gluconate uptake: gluconate I and gluconate II.
 +
            </div>
  
 +
            <!--            BBa_K3137006-->
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 +
            <div class="fb_48">
 +
                <b>BBa_K3137006</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>abpAB</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 3803bp resistant protein<br/>
 +
                <i>AbpAB</i> are two genes of the <i>E.coli</i> genome that express resistant proteins that are
 +
                resistant to T2,T4,T7
 +
                and λ phages. We obtained <i>abpA</i> and <i>abpB</i> by PCR from the genome of ?<i>E.coli</i> BL21 and
 +
                constructed
 +
                recombinant plasmid that connected <i>abpA</i> and <i>abpB</i> at the same time, then used IPTG to
 +
                induce the
 +
                expression of resistant protein and verified the resistant function of the protein.
 +
            </div>
  
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<div class="column full_size">
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            <!--            BBa_K3137007-->
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 +
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 +
                <b>BBa_K3137007</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>rzpD</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 462bp<br/>
 +
                Overexpression of <i>rzpD</i> causes abnormal biofilm architecture.
 +
            </div>
  
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
            <!--            BBa_K3137008-->
−
<p>The information needed to initially create a part on the Registry is:</p>
+
            <div class="split_small"></div>
−
<ul>
+
            <div class="fb_48">
−
<li>Part Name</li>
+
                <b>BBa_K3137008</b>
−
<li>Part type</li>
+
            </div>
−
<li>Creator</li>
+
            <br/>
−
<li>Sequence</li>
+
            <div class="fm_22">
−
<li>Short Description (60 characters on what the DNA does)</li>
+
                <i>yhjH</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 768bp<br/>
−
<li>Long Description (Longer description of what the DNA does)</li>
+
                <i>YhjH</i> protein contains a EAL domain to catalyze c-di-GMP into GMP, so as to regulate the levels of
−
<li>Design considerations</li>
+
                c-di-GMP.
−
</ul>
+
            </div>
  
−
<p>
+
            <!--            BBa_K3137009-->
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
+
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137009</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>nuoE</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 501bp<br/>
 +
                <i>NuoE</i> is part of the soluble fragment of NADH dehydrogenase I, which represents the electron input
 +
                part
 +
                of the enzyme.
 +
            </div>
  
−
</div>
+
            <!--            BBa_K3137010-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137010</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>T7 terminator</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 48bp terminator<br/>
 +
                T7 terminator is used to quantify the level of expression in <i>E. coli</i>.
 +
            </div>
  
 +
            <!--            BBa_K3137011-->
 +
            <div class="split_small"></div>
 +
            <div class="fb_48">
 +
                <b>BBa_K3137011</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                <i>gfp</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 717bp reporter<br/>
 +
                The <i>gfp</i> can express green fluorescent protein, which can be used as a marker gene in the process
 +
                to report the number of cells.
 +
            </div>
  
−
<div class="clear extra_space"></div>
+
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−
<div class="line_divider"></div>
+
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<div class="clear extra_space"></div>
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            <div class="fb_48">
 +
                <b>BBa_K3137011</b>
 +
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                codes for<i>protegrin-1</i>&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp (anti-microbial peptide) 60bp
 +
                antibacterial peptides<br/>
 +
                <i>Protegrin-1</i> coding region. <i>Protegrin-1</i> is an '18-residue beta-sheet peptide isolated from
 +
                porcine
 +
                leukocytes with antimicrobial activity against a broad range of microorganisms.' It has its effect by
 +
                pore membrane disruption and possibly also by effects such as activation of membrane-damaging proteases
 +
                and has anti-microbial activity against <i>E. coli</i>.
 +
            </div>
  
−
<div class="column full_size">
 
−
<h3>Part Table </h3>
 
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
            <!--第二部分-->
 +
            <div class="split"></div>
 +
            <div class="fb_72"><b>Composite Part</b></div>
  
−
</html>
+
            <div class="split_small"></div>
−
<groupparts>iGEM19 JiangnanU_China</groupparts>
+
            <div class="fb_48">
−
<html>
+
                <b>BBa_K3137013</b>
−
</div>
+
            </div>
 +
            <br/>
 +
            <div class="fm_22">
 +
                report circuit &nbsp &nbsp 1666bp
 +
            </div>
  
 +
            <div class="split_small"></div>
 +
            <img src="https://static.igem.org/mediawiki/2019/1/19/T--JiangnanU_China--parts_1.png"
 +
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            <div class="fm_22">
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                The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min),
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                there is an inducible promoter <i>PputA</i> and a green fluorescent protein gene <i>gfp</i>. In outbreak
 +
                period (about
 +
                20 min), there is an inducible promoter <i>PglcF</i>, and a red fluorescent protein gene <i>mCherry</i>.
 +
                When <i>E. coli</i> is infected by a phage, both report and respond gene circuits are induced to express
 +
                at the
 +
                same time. When a phage infects <i>E. coli</i> into the incubation period (about 5 min), the inducible
 +
                promoter
 +
                <i>PputA</i> will induce the bacteria to express the resistant protein and the green fluorescent protein
 +
                gene
 +
                <i>gfp</i>.
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                If the resistant protein successfully resists phage infection, the inducible promoter <i>PglcF</i> will
 +
                not
 +
                induce the expression of downstream gene. If the resistant protein fails to kill phages, it means that
 +
                phages will continue to infect bacteria, when it comes to the outbreak period (around 20 min) of phage
 +
                infection, the inducible promoter <i>PglcF</i> will activate the red fluorescent protein gene
 +
                <i>mCherry</i> and the
 +
                expression of downstream toxic protein gene so that the bacteria will display red fluoresce and lyse
 +
                cells before the assembly of phages.
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                <b>BBa_K3137014</b>
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                respond circuit &nbsp &nbsp 5097bp
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            <img src="https://static.igem.org/mediawiki/2019/f/fb/T--JiangnanU_China--parts_2.png"
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            <div class="split_small"></div>
  
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            <div class="fm_22">
 +
                The respond gene circuit is composed of an inducible promoter <i>PputA</i> and anti-phage protein genes of the
 +
                incubation period (about 5 min), and an inducible promoter <i>PglcF</i> and a toxic protein gene <i>protegrin-1</i> of
 +
                the outbreak period (about 20 min).
 +
                When <i>E. coli</i> is infected by a phage, both report and respond gene circuits are induced to express at the
 +
                same time. When a phage infects <i>E. coli</i> into the incubation period (about 5 min), the inducible promoter
 +
                <i>PputA</i> will induce the bacteria to express the resistant protein <i>abpAB</i> and <i>gntR</i>, and the green
 +
                fluorescent protein gene <i>gfp</i>.
 +
                If the resistant proteins successfully resist phage infection, the inducible promoter <i>PglcF</i> will not
 +
                induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that
 +
                phages will continue to infect bacteria, when it comes to the outbreak period (around 20 min) of phage
 +
                infection, the inducible promoter <i>PglcF</i> will activate the red fluorescent protein gene <i>mCherry</i> and the
 +
                expression of downstream toxic protein gene <i>protegrin-1</i> so that the bacteria will display red fluoresce
 +
                and lyse cells before the assembly of phages.
 +
            </div>
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            <a href="#head"><img src="https://static.igem.org/mediawiki/2019/2/24/T--JiangnanU_China--host_back.png"
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                                alt="back" style="width: 6%;height:auto;margin-left: 46%"></a>
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Revision as of 14:22, 18 October 2019

JiangNan

Basic Part
BBa_K3137000

rsmH&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp50bp constitutive promoter
RsmH can detect the intensity of the fluorescence of gfp.
BBa_K3137002

PputA&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 50bp inducible promoter
PputA is the inducible promoter that When E.coli is infected by a phage, both report and respond gene circuits are induced to express at the same time. When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to express resistant protein and display green fluorescence.
BBa_K3137003

PglcF &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 50bp inducible promoter
PglcF is the inducible promoter that works when phages infect bacterial for around 20 min. If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins cannot kill phages, the phages will continue to infect. When phages infect bacteria into the outbreak period (about 20 min), the inducible promoter PglcF will induce expression of the red fluorescent protein gene mCherry and the downstream toxic protein gene protegrin-1, which will make the bacteria display red fluorescence and lyse cells before the assembly of phages.
BBa_K3137004

mCherry&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 708bp marker gene
The mCherry can express red fluorescent protein and it is used as a marker gene in the process of reporting the number of cells.
BBa_K3137005

gntR&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 996bp
The Gluconate repressor gntR, is a transcription factor that negatively regulates the operon involved in the catabolism of d-gluconate via the Entner-Doudoroff pathway and represses genes involved in the different systems related to d-gluconate uptake: gluconate I and gluconate II.
BBa_K3137006

abpAB&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 3803bp resistant protein
AbpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of ?E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.
BBa_K3137007

rzpD&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 462bp
Overexpression of rzpD causes abnormal biofilm architecture.
BBa_K3137008

yhjH&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 768bp
YhjH protein contains a EAL domain to catalyze c-di-GMP into GMP, so as to regulate the levels of c-di-GMP.
BBa_K3137009

nuoE&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 501bp
NuoE is part of the soluble fragment of NADH dehydrogenase I, which represents the electron input part of the enzyme.
BBa_K3137010

T7 terminator&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 48bp terminator
T7 terminator is used to quantify the level of expression in E. coli.
BBa_K3137011

gfp&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp 717bp reporter
The gfp can express green fluorescent protein, which can be used as a marker gene in the process to report the number of cells.
BBa_K3137011

codes forprotegrin-1&nbsp &nbsp &nbsp &nbsp &nbsp &nbsp &nbsp (anti-microbial peptide) 60bp antibacterial peptides
Protegrin-1 coding region. Protegrin-1 is an '18-residue beta-sheet peptide isolated from porcine leukocytes with antimicrobial activity against a broad range of microorganisms.' It has its effect by pore membrane disruption and possibly also by effects such as activation of membrane-damaging proteases and has anti-microbial activity against E. coli.
Composite Part
BBa_K3137013

report circuit &nbsp &nbsp 1666bp
1666bp
The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA and a green fluorescent protein gene gfp. In outbreak period (about 20 min), there is an inducible promoter PglcF, and a red fluorescent protein gene mCherry. When E. coli is infected by a phage, both report and respond gene circuits are induced to express at the same time. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant protein and the green fluorescent protein gene gfp. If the resistant protein successfully resists phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant protein fails to kill phages, it means that phages will continue to infect bacteria, when it comes to the outbreak period (around 20 min) of phage infection, the inducible promoter PglcF will activate the red fluorescent protein gene mCherry and the expression of downstream toxic protein gene so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.
BBa_K3137014

respond circuit &nbsp &nbsp 5097bp
5097bp
The respond gene circuit is composed of an inducible promoter PputA and anti-phage protein genes of the incubation period (about 5 min), and an inducible promoter PglcF and a toxic protein gene protegrin-1 of the outbreak period (about 20 min). When E. coli is infected by a phage, both report and respond gene circuits are induced to express at the same time. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA will induce the bacteria to express the resistant protein abpAB and gntR, and the green fluorescent protein gene gfp. If the resistant proteins successfully resist phage infection, the inducible promoter PglcF will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the outbreak period (around 20 min) of phage infection, the inducible promoter PglcF will activate the red fluorescent protein gene mCherry and the expression of downstream toxic protein gene protegrin-1 so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.
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