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Kyle has been a strong asset throughout the entire iGEM project. His strong interest in the project has meant that he constantly pushed us toward a better project with expert facts and deliberations, not really regarding how long each individual suggestion would take, which resulted in many long nights, but we definitely wouldn't have wanted to be without them.  
 
Kyle has been a strong asset throughout the entire iGEM project. His strong interest in the project has meant that he constantly pushed us toward a better project with expert facts and deliberations, not really regarding how long each individual suggestion would take, which resulted in many long nights, but we definitely wouldn't have wanted to be without them.  
 
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<b>Kyle Rothschild-Mancinelli (iGEM Alumnus and PhD student) </b><br>
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<p><b>Max Van't Hof (Ph.D Student at DTU and previous iGEM Participant)</b><br>
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Revision as of 13:42, 17 October 2019

Attributions

We want to say thank you to all the wonderful men and women who have helped us during this project. We couldn't have done it without you.

Team members

Marcus Medom Ryding

As the main responsible for the drylab side of the project, I established our choice of model and wrote the software to build models and sample promoters from them. I was also involved in our molecular design work, where I worked to promote modularity and standards compliance, both in the selection and design behind individual parts, but especially on higher levels of abstraction, in our designs and assembly strategies for devices and backbones, aiming to embed our work into the growing ecosystem of Type IIS assembly standards.

Helena Utzon

Throughout this project I have mainly participated in the wet-lab through work with filamentous fungi and fermentation, though I also lend a hand whenever it was needed in the molecular part of the wet-lab.
Furthermore, I worked a lot with outreach, including being head of the social media group, teaching high-school students, and working with integrated human practices. Finally, I have also helped arrange and host several team-building activities.

Louise Almer

In the process of this project, I have mostly worked with the fungi and fermentations but I have also stepped in other places where it has been needed.
I have also partaken in the design and set-up of the wiki. Additionally, I have planned and attended many of the outreach activities, ranging from integrated human practices to non-integrated human practices and education of high school students.

Mikkel Hansen

been a big part of the promoter design and assisted in the drylab part of the project, I have worked a lot with the fungi and done a huge amount of fungal transformations. I have done work in education and a bit of integrated human practice. I have helped with the molecular biology when needed.

Jacob Mejlsted

During the project, I have been the main responsible for the handling of safety forms and the organization of the laboratory. Over the summer, I was also the main responsible for the cloning part of the laboratory as well as organizing and systematizing the samples, storage and notations that we have been using.

Philip George Hau Sørensen

I have been the main responsible for task delegation and administrative work. I have also been heavily involved in the experimental design for this project, designing both parts and cloning strategies. In addition to this, I have also assisted Jacob in the lab with cloning work during the summer. Lastly, I have been involved with the economic side of the project, contributing to fundraising efforts and budgeting for the team.

Philip Thomsen

During the project my primary focus has been on the fungal work; preparing protoplasts, fungal transformations, etc. However as a jack of all trades, I have been working in the lab wherever the need has been the greatest, be it designing parts, molecular work, or whatever might be required. Additionally, I have helped secure funding for the project.

Mathias Laursen

Elisavet Angeli

As a member of the drylab group, my role was mostly to search relevant literature and analyse different datasets that would be used in the development of the software and finally in the synthesis of our library of promoters. Furthermore, I assisted in the wet lab part of the project when needed.

Michelle Lind Østrup

I was the main responsible in the development of the wiki. Almost all code and all illustrations have been created by or at least been passed by me. Also if you like the t-shirts and our banners, then I designed those as well. If you didn’t like them…then I still made them.

Supervisors

Christopher Workman (Associate Professor, DTU Bioengineering)
Chris is the core of the DTU Biobuilders. With all of his experience with previous iGEM teams, he has been able to guide us through all the rough patches of the project as well as delivering critical input for both the work in the lab and in the coding department.

Henrik Toft Simonsen (CEO of Mosspiration Biotech IVS & Associate professor at DTU Bioengineering)
As the CEO of Mosspiration Biotech IVS & Associate professor at DTU, Henrik is a great supervisor who knows how to motivate us during crunch times. He's able to bring an almost business-like aspect to the project which makes the project seem more relevant than ever and we are happy to have him on board.

Kyle Rothschild-Mancinelli (iGEM Alumnus and Ph. D. Student)
Kyle has been a strong asset throughout the entire iGEM project. His strong interest in the project has meant that he constantly pushed us toward a better project with expert facts and deliberations, not really regarding how long each individual suggestion would take, which resulted in many long nights, but we definitely wouldn't have wanted to be without them.

Wetlab

Christopher Workman (Associate Professor, DTU Bioengineering)
Provided expert advice and overall feedback throughout the project.

Kyle Rothschild-Mancinelli (iGEM Alumnus and Ph. D. Student)
Suggested solutions and methods during the entire project.

Jakob Blæsbjerg Hoof (DTU Bioengineering)
Expert on Aspergilli and generously provided us with the Aspergillus niger strain ACTT1015 and the plasmids containing PyrG and the homology regions.

Jakob Kræmmer Rendsvig (Ph.D. Student at DTU Bioengineering)
Most generously provided feedback, plasmids, and guidance throughout the project. His help has been invaluable for our successes.

Fabiano Jares Contesini (DTU Bioengineering)
Provided us with a protease deficient Aspergillus niger strain.

José Luis Martinez Ruiz (DTU Bioengineering)
Helped us with advice on fermentation in bioreactors.

Irene Hjorth Jacobsen (Ph.D. Student at DTU Bioengineering)
Offered invaluable advice on how to run the bioreactors.

Clara Navarrete Román (Postdoc at DTU Bioengineering)
Helped us with using the BioLector.

Zofia Dorota Jarczynska (Postdoc at DTU Bioengineering)
Provided advice on how to optimize the protoplasting protocol and provided feedback, advice, and support with regards to fungal transformation and cloning.

David Lokjær Faurdal (DTU Biobuilders 2018)
Helped with the execution of the BioBrick Tutorial 2019 and occasional guidance and help in the lab.

Lina Lyng Sigfredsson Nielsen (DTU Biobuilders 2018)
Helped with the BioBrick Tutorial 2019.

Marie Millgaard (Msc. Student at DTU)
Helped with the BioBrick Tutorial 2019.

Joen Haahr Jensen (DTU Biobuilders 2018)
Developed systems for organizing our DNA inventory in 2018 that was also used in 2019.

Tina Johansen (Lab Manager at DTU Bioengineering)
Has helped with ordering reagents on behalf of our team, and managing the booking system for the bioreactors we were using.

Amalie Gro Kloster (Laboratory Technician at DTU Bioengineering)
Has helped with ordering reagents on behalf of our team, and restocking the lab with consumables. She also provided occasional advice on different lab protocols.

Jannie Felskov Agersten (Laboratory Technician at DTU Bioengineering)
Has helped with guidance on some of the SOPs and protocols for our lab.

Marzanna Pulka-Amin (Laboratory Technician at DTU Bioengineering)
Has helped up with preparing a teaching-lab for us to host the BioBrick Tutorial in.

Trine Helena Brodersen (Lab Trainee at DTU Bioengineering)
Has helped us by guiding us in how to use certain equipment in the lab.

Drylab

Christopher Workman (Associate Professor, DTU Bioengineering)
Proposed the initial idea of homology models for promoter sequences, and provided invaluable guidance and discussions throughout the development process.

Human practices

Kenneth Bruno (Principal Scientist at Zymergen)
Provided crucial feedback at the start of the project about the choice of aspergillus strain and methods to determine promoter strength. Furthermore, he provided information about the needs of a promoter library in the industry.

Grayson Wawrzyn (Scientist at Zymergen)
Gave us important feedback to the project.

Benjamin Schwetz (Information Scientist at Novozymes)
Invited us to visit Novozymes’ facilities in Lyngby and arranged talks with different departments to learn more about the applications of enzymes.

Mikako Sasa (Senior Scientist at Novozymes)
Talked to us about the use of filamentous fungi in industrial applications.

Alfred Birkegaard Hansted (Collaborative Innovation Specialist at Hello Science)
Introduced the many aspects of innovation and entrepreneurship in the biotechnological space.

Pengfei Tian (Senior Data Scientist at Novozymes)
Talked to us about visualisation of scientific topics as a part of research with his focus on 3D models of enzymes.

Merete Møller Engelsen (Science Manager at Novozymes)
Talked to us about real-world applications of heterologous proteins that can be produced in filamentous fungi.

Mary Ann Stringer (Senior Scientist at Novozymes)
Provided feedback on which types of promoters are really needed in industry.

Kirk Matthew Schnorr (Senior Scientist at Novozymes)
Provided feedback on the advantages, disadvantages, application, and methods of our project.

Peter Richard (Principal Scientist at VTT)
Provided feedback on our project, more specifically how to test our constructs in a way that ensures maximal usefulness to others.

Edyta Szewczyk (Senior Scientist at Bolt Threads)
Gave us a lot of feedback and ideas for the best ways to perform an experiment and what to look for in our product.

Outreach

Camilla Lykke Hansen (DTU communication)
Helped us organize our stand at Science Expo.

Anne Ringgaard Jørgensen (DTU Project coordination)
Helped us acquire DTU branded t-shirts.

Mårten Flø Jørgensen (Science Teacher at Bagsværd High School)
Allowed us to visit his high school class and talk to aspiring young biologists about making the world a better place with synthetic biology.

Morten Eskildsen (Science Teacher at Birkerød High School)
Allowed us to visit his high school class and talk to aspiring young biologists about making the world a better place with synthetic biology.

Tenna Gram Rasmussen (Msc. Student at DTU)
Helped us at the annual DTU open house.

Felix Boel Pedersen (Previous iGEM Participant from SDU)
Held a lecture at the BioBrick Tutorial.

Max Van't Hof (Ph.D. Student at DTU and previous iGEM Participant)
Held a lecture at the BioBrick Tutorial.

Chrysillis Magaard Polhaus (Previous iGEM Participant from DTU)
Held a lecture at the BioBrick Tutorial.

Economy

Malene Bonné Meyer (DTU Bioengineering)
Help with budgeting and fundraising.

Chen Chou Hellfach Hansen (DTU Project economy coordinator)
Help with budgeting and fundraising.

Elisabeth Boelskifte (DTU Bioengineering)
Help with budgeting and fundraising.

Collaborations

Teaghan Cowles (Brown-Stanford-Princeton 2019)
Our contact person on their team and coordinator of the collaboration.

Cale Ezra Lester (Brown-Stanford-Princeton 2019)
Co-point person on the collaboration to test our software.

Jonas Gockel (Uppsala-University iGEM team 2019)
Contact person on the Uppsala team.

Thea Amalie Hvidtfeldt (SDU-Denmark 2019)
Contact person on the SDU-Denmark team who sent their plasmid to help us with the cloning of kanamycin resistance

Stine Degn Espersen (SDU-Denmark 2019)
Contact person on the SDU-Denmark team who sent their plasmid to help us with the cloning of kanamycin resistance

Danish iGEM Network

Together with the GEM teams from SDU and Copenhagen we have started the creation of the Danish iGEM network. We are looking forward to continuing our work to help future Danish iGEM-teams.

Grants

The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden

DTU Blue Dot
DTU Biobuilders is a BlueDot project, meaning that it is sponsored by DTU, and part of a larger network of students. All projects associated with BlueDot aims to develop a sustainable solution to a problem. During the projects the students work together across semesters, curricula and fields of study - www.dtu.dk

Novo Nordisk fonden
The Novo Nordisk Foundation uses its independence, flexibility and long-term perspective to promote world-class research. In this way, the Foundation contributes to developing solutions that address the pressing challenges of the future – to benefit both individuals and society as a whole - novonordiskfonden.dk

Otto Mønsted fonden
Otto Mønsted Fonden has supported and contributed to the Danish Trade and industry, since its foundation in 1916. They also supports research and educational science within the technical science and commercial disciplines - www.ottomoensted.com

Sponsors

The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience

DTU
The Technical University of Denmark is a technical elite university with international reach and standards. Its mission is to develop and utilize natural and technical sciences to benefit society. The university houses 10,300 students and 5,800 employees focused on education, research, consultancy for the public sector and innovation, which contributes to increased growth and welfare - www.dtu.com

BioNordica
The BIONORDIKA GROUP was founded from 1986-1989. They offer a wide range of products and services focus in the areas of Cell & Molecular Biology, Immunology & Diagnostics, Instruments & Disposables and Custom Products & Projects - bionordika.dk.

Eurofins Genomics
Eurofins Genomics is globally known for its innovative and customised technologies in the life science industries and academic research institutions. It is an international provider of DNA sequencing services, oligonucleotide synthesis products and bioinformatic services - eurofinsgenomics.eu

Qiagen
Qiagen is a provider of sample and assay technologies for molecular diagnostics, applied testing, academic and pharmaceutical research - www.qiagen.com

NEB New England biolabs
New England Biolabs is a recognized world leader in the discovery, development and commercialization of recombinant and native enzymes for genomic research - www.neb.com

IDT Integrated DNA technologies
IDT develops, manufactures, and markets nucleic acid products that support the life sciences industry in the areas of academic and commercial research, agriculture, medical diagnostics, and pharmaceutical development - www.idtdna.com

Twist bioscience
Twist Bioscience use their synthetic DNA tools to make the world a better plays through their customers in fields like medicine, agriculture, industrial chemicals, and data storage - twistbioscience.com

The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience