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Revision as of 00:12, 15 October 2019
Rediscovering bioproduction
genSwitch is a genetic circuit able to make E.coli recognize blue led light and switch between two different states of genetic activation
What is bioproduction?
Bioproduction is a process used in industries that generates useful products through microorganisms. The most common inductor used in bioproduction are chemical compounds (like IPTG and arabinose) that are, not only expensive, but also lacks fine control of induction. Our goal is to built a genetic circuit that can switch between two different states of activation with just a single input of blue led light. The circuit was built in E.coli and works based on inhibition handles and recombinases that can invert the promoter region according to the presence and absence of the same type of blue light. In addition, fluorescent proteins such as GFP, RFP and YFP function as outps for our circuit. We also compared the efficiency between chemical induced promotors and light-induced ones by evaluating strength and lack. Finally, with our circuit, bioproduction can become more practical, controlled and economical.
Why use light?
Light is a fine, precise inductor, as the specificity of the signal is in the amissor, the wavelength and intensity, and in the receptor, in our case, a protein, that recognize a specific wavelength ortogonali. Besides that, light can be activated instantaneously, simply by turning on it’s source. To stop the induction, it’s not necessary to change the culture media or wash the culture, a process that can make some bioproduction impossible. Using light opens new possibilities for this industry.
Why make a switch?
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Modeling
Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.
Gold Medal Criterion #3
Convince the judges that your project's design and/or implementation is based on insight you have gained from modeling. This could be either a new model you develop or the implementation of a model from a previous team. You must thoroughly document your model's contribution to your project on your team's wiki, including assumptions, relevant data, model results, and a clear explanation of your model that anyone can understand.
The model should impact your project design in a meaningful way. Modeling may include, but is not limited to, deterministic, exploratory, molecular dynamic, and stochastic models. Teams may also explore the physical modeling of a single component within a system or utilize mathematical modeling for predicting function of a more complex device.
Best Model Special Prize
To compete for the Best Model prize, please describe your work on this page and also fill out the description on the judging form. Please note you can compete for both the Gold Medal criterion #3 and the Best Model prize with this page.
You must also delete the message box on the top of this page to be eligible for the Best Model Prize.
Inspiration
Here are a few examples from previous teams: