Giu's Stellar Project

Rediscovering bioproduction

genSwitch is a genetic circuit able to make E.coli recognize blue led light and switch between two different states of genetic activation

What is bioproduction?

Bioproduction is a process used in industries that generates useful products through microorganisms. The most common inductor used in bioproduction are chemical compounds (like IPTG and arabinose) that are, not only expensive, but also lacks fine control of induction. Our goal is to built a genetic circuit that can switch between two different states of activation with just a single input of blue led light. The circuit was built in E.coli and works based on inhibition handles and recombinases that can invert the promoter region according to the presence and absence of the same type of blue light. In addition, fluorescent proteins such as GFP, RFP and YFP function as outps for our circuit. We also compared the efficiency between chemical induced promotors and light-induced ones by evaluating strength and lack. Finally, with our circuit, bioproduction can become more practical, controlled and economical.

Why use light?

Light is a fine, precise inductor, as the specificity of the signal is in the amissor, the wavelength and intensity, and in the receptor, in our case, a protein, that recognize a specific wavelength ortogonali. Besides that, light can be activated instantaneously, simply by turning on it’s source. To stop the induction, it’s not necessary to change the culture media or wash the culture, a process that can make some bioproduction impossible. Using light opens new possibilities for this industry.

Why make a switch?

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique. Duis cursus, mi quis viverra ornare, eros dolor

★ ALERT!

This page is used by the judges to evaluate your team for the medal criterion or award listed below.

Delete this box in order to be evaluated for this medal criterion and/or award. See more information at Instructions for Pages for awards.

Improve a Previous Part or Previous Project

For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part's main page on the Registry. Please include a link to your improved part's Registry page on this page.

Gold Medal Criterion #2

Improve a Previous Part - Standard Tracks: Convince the judges that you have created a new BioBrick Part that has a functional improvement upon an existing BioBrick Part. You must perform experiments with both parts to demonstrate this improvement. Clearly document the quantitative experimental characterization data on the Part's Main Page on the Registry for both the existing and new parts (see the Registry Document Parts page for instructions).

Both the existing and new part must be RFC10 or Type IIS compatible. The sequences of the new and existing parts must be different. The existing part must NOT be from your 2019 part number range and must be different from the part you used in Bronze #4. The new part you create must also be different from the new part documented in Silver #1. Please see the Measurement Resources page for more information about experimental characterization data.

Improve a Previous Project - Special Tracks: Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.