Team:USAFA/Attributions

 

Attributions

Every member of the 2019 USAFA iGEM team played a critical role in helping with this project. In addition, we would like to thank the people who assisted in this project and gave guidance to the USAFA iGEM team.

People

  • Captain Michael Barnhart and Dr. Jordan Steel were our main advisers during our project. They helped the team from the beginning with experimental design. They negotiated our funding for the Jamboree and taught the team the uses of methods and materials in the laboratory.
  • Captain Carlee Koutnik inspired the main idea for our iGEM project and presented our poster at the Team Aerospace Operational Solutions.
  • Captain John Sitko advised and mentored the iGEM team in the laboratory with his valuable expertise in PFOS detection.
  • Major Leonardo Somera, Major Marisa Romero, and Dr. Katherine Bates lent us their useful and valuable real-life application advice regarding our ideas in the initial steps of our project.

  • Dr. Anthony Arment helped us with giving us direction and guidance in the late stages and future ideas for our project.
  • Colonel Steve Hasstedt and Dr. David Hale have been constant supporters for our iGEM program and Cadet Research program.
  • Dr. Don Veverka have allowed us to use his lab space, give helpful expertise, and assisted us with allocation and funding.

Organization

  • The Air Force Research Labs at Wright-Patterson Air Force Base, Ohio granted us materials, funding, lab space, organisms, and mentors. We would like to especially thank Dr. Chia Hung for guiding the molecular cloning aspects of this project and Dr. Jonathan Sharp for providing us with Rhodococcis jostii RHA1 strain.
  • The Colorado School of Mines provided our iGEM team with plasmids and reagents to work on for our project.
  • Funding was provided by the US Office of the Surgeon General, the Life Science Research Center at USAFA, and the Biology Department at USAFA.

Other Citations

  • Cappelletti, Martina et al. “Growth of Rhodococcus sp. strain BCP1 on gaseous n-alkanes: new metabolic insights and transcriptional analysis of two soluble di-iron monooxygenase genes.” Frontiers in microbiology vol. 6 393. 12 May. 2015, doi:10.3389/fmicb.2015.00393
  • Colosi, L. M., Pinto, R. A., Huang, Q., & Weber, W. J. J. (2009). Peroxidase‐mediated degradation of perfluorooctanoic acid. Environmental Toxicology and Chemistry: An International Journal, 28(2), 264-271.
  • Kwon, B. G., Lim, H. J., Na, S. H., Choi, B. I., Shin, D. S., & Chung, S. Y. (2014). Biodegradation of perfluorooctanesulfonate (PFOS) as an emerging contaminant. Chemosphere, 109, 221-225.
  • Mitchell, S. M., Ahmad, M., Teel, A. L., & Watts, R. J. (2013). Degradation of perfluorooctanoic acid by reactive species generated through catalyzed H2O2 propagation reactions. Environmental Science & Technology Letters, 1(1), 117-121.
  • Mohn, W. W., Wood, T. K., Liu, J., Sales, C. M., Sharp, J. O., Eltis, L. D., … LeBlanc, J. C. (2007). An Inducible Propane Monooxygenase Is Responsible for N-Nitrosodimethylamine Degradation by Rhodococcus sp. Strain RHA1. Applied and Environmental Microbiology, 73(21), 6930–6938.
  • Yi, L. B., Chai, L. Y., Xie, Y., Peng, Q. J., & Peng, Q. Z. (2016). Isolation, identification, and degradation performance of a PFOA-degrading strain. Genet Mol Res, 15(2), 235-246.