Week 34 (19th-25th of August)

19th August 2019

1. REPETITION OF COLONY PCR

Team members: Mads and Signe

The colony PCR was done again on as problems appeared in the colony PCR done in the last couple of days. The colony PCR was done on the following:


Plate nr.	Construct
3	HuLHCGR - Li + sfGFP
4	HuLHCGR + pccw12 + X3A
7	XlHCGR - Li + sfGFP + pccw12 + X3A
-	Neg. control
-	Positive control* (Clony 8 - X12A - TEF1 + BAX)

*The positive control was from the kill switch group. This was used as we knew the colony was positive and should therefore show up on the gel if everything worked as it should.

Materials

The sample volumes written is for one sample


Material	Volumen [µL]
MangoMix	5
F-primer	0.5
R-primer	0.5
mQ-water	4
In total	10

Procedure

The colony PCR was done according to the normal procedure, but we tried to use MangoMix instead of making normally mastermix.
3 colonies was taken pr. Plates* (only one colony from the positive control plate)
*one sample with colonies from plate 7 went wrong and we did not use if further on.
Loading order: 4,4,4,7,7,3,3,3, negative control, positive control (colony 8 on plate X12A-TEF1+BAX from kill switch work).