Team:Thessaloniki/Medals

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Bronze

  • Registration and Giant Jamboree Attendance

    We have successfully registered for the iGEM competition in May, designed our project and had an educational season full of new experiences. Our team members’ registration has been completed in August and we are looking forward to presenting our work in the Giant Jamboree.

  • Competition Deliverables

    • We have a fully updated and functional wiki, including everything we have accomplished during the iGEM season.
    • We are going to present our poster in the upcoming Giant Jamboree.
    • Our Project’s Presentation will take place on Saturday the 2nd of November in Room 313.
    • We have completed the Judging Form.

  • Attributions

    We gave credit to everyone that contributed to our project and we state the work done by them and by the team members in the Attributions wiki page. We are deeply thankful to everybody that supported us.

  • Project Inspiration and Description

    We submitted the project’s inspiration and description for the first time in June. Since then we made small changes to improve the readability of the text. You can learn more on our Description & Inspiration wiki page.

  • Characterization / Contribution

    We managed to provide quantitative experimental characterization data to the following parts of the Registry of Standard Biological Parts:

    All of the information and characterization data have been documented on the respective parts’ Main Pages on the Registry.

Silver

  • Validated Part / Validated Contribution

    We managed to validate the following parts of our design, that are related to our project:

    All of the information and characterization data have been documented on the respective parts’ Main Pages on the Registry.

  • Collaboration

    We did collaborate with seven other iGEM teams. Among these collaborations, we validated the project of iGEM Groningen. Additionally, we created an electronic platform to facilitate communication between the Greek teams. We describe the give and take relationship in detail in Collaborations wiki page.

  • Human Practices

    During this iGEM season, our team went way beyond the lab. We came in contact with a variety of different social groups, ranging from public groups to the scientific community and industry. We “reached to the world, for the world to reach back” using a variety of different techniques. Participating in conferences, appearing on radio shows and producing outstanding videos are some of the approaches we used. You can find everything concerning human practices in the Human Practices page.

Gold

  • Integrated Human Practices

    To facilitate future communication between iGEM teams and society we have created the “DESMOS” protocol. DESMOS includes instructions on how to pinpoint the external risks an iGEM team might face while developing their project. Additionally, we implemented some experimental guidelines on how to demonstrate the project to different audiences. We used our protocol to raise risks and present our work to academics of the field. The timeline presenting the most influential meetings can be found on our individual Integrated Human Practices page.

  • Improve a Previous Part / Project

    We managed to provide functional improvement of the following part of the Registry of Standard Biological Parts.

    The new part that our team created as an improvement of the latter part is the following: All of the information and characterization data have been documented on the respective parts’ Main Pages on the Registry:

  • Model Your Project

    Our whole DNA Strand Displacement circuit was designed based on insight gained from our model. Specifically, we were able to design the system and the DNA sequences we used experimentally. Additionally, we implemented Molecular Dynamic simulations in our model, to calculate a theoretical value for the binding free energy of the Protein-DNA complex. A detailed analysis of our modeling work can be found on our Model page.

  • Demonstration of Your Work

    In our proof of concept experiments, we developed a functional DNA Strand Displacement circuit which was used along with either of two transcription factors, NF-κB and ELK1 for different binding sequences. Our circuit was able to produce measurable quantitative output signal, relative to each transcription factor binding affinity to their consensus ligand DNA sequence and to Single Nucleotide Polymorphisms of it. Furthermore, we successfully cloned our DNA Gates into PSB1C3 plasmids and presented that our circuit can function similarly as expected with the use of plasmid derived DNA Gates. Finally, we integrated our circuit into a Field-Effect Transistor gate of our own design and construction, capable of producing a response relative to the binding affinity of proteins to target probe DNA sequence. In our Results section of our Wiki, we have provided sufficient documentation and measurement data that our circuit works as expected.