Team:Sydney Australia/Collaborations
Collaborations
Macquarie University iGEM Team ♥
We performed a fluorescence assay testing the GFP expression of three different riboswitches based systems, each in two different conditions. This involved transforming plasmids they gave us into DH5α cells, growing them up on plates, transferring cells to liquid cultures, serially diluting those culture in a 96 well plate then measuring their fluorescence over 72 hours. They had already performed this fluorescence assay, and wanted us to validate their results.
In return, they used their own cells and protocol to transform and induce one of our constructs, pET-28c(+):PsiD. Macquarie then then ran it on an SDS-PAGE gel to see if there was protein expression. We had been having a lot of trouble with protein expression, and wanted to see if it would work with different cells and a different protocol.
UNSW iGEM Team
The UNSW iGEM team organised a symposium ‘A Sustainable Future Using Synthetic Biology’, including an excellent panel of scientists and social scientists.
We presented our project at the symposium, which provided them with content, and they allowed us to practice our Jamboree talk. It also gave us the chance to meet the other Sydney teams and hear the panel discussion.
Dusselfdorf iGEM Team
We participated in their postcard collaboration. They helped in our outreach and education by allowing us to spread information about our project around the world. We helped them by participating in their outreach. Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
