Team:Sheffield/Medals
Bronze
We have registered for the team and plan to attend the Jamboree.
02 Competition Deliverables
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Wiki
https://2019.igem.org/Team:Sheffield -
Poster
We will be attending the Jamboree and defending our poster. -
Presentation
We will be attending the jamboree and presenting our project -
Judging Form
We have completed and submitted the judging form by the deadline. -
Safety Forms
We have already submitted all the necessary safety forms by the appropriate deadline.
03 Attributions
https://2019.igem.org/Team:Sheffield/Attributions
04 Project Inspiration and Description
https://2019.igem.org/Team:Sheffield/Description
05 Contribution
We have created an affordable, Do-It-Yourself, open source microplate reader, that would allow any lab to make and service their own for a very low price. This would allow synthetic biology projects to screen a number of mutants or strains with new plasmids for any potential growth defects at the same time. For the full documentation of our microplate reader, please visit:
https://2019.igem.org/Team:Sheffield/Hardware,
https://2019.igem.org/Team:Sheffield/Contribution.
Silver
For more information, please visit: https://2019.igem.org/Team:Sheffield/Hardware, and https://2019.igem.org/Team:Sheffield/Demonstrate.
02 Collaboration
https://2019.igem.org/Team:Sheffield/Collaborations
03 Human Practices
The questions our Human Practices sought to address, were what kind of experiences microplate readers have when using current readers in the market. Why they use them, what issues they wish would be addressed, what they love about them. We wanted to focus on both hardware and software. We engaged with University lab researchers, PIs, community labs, and more. For a full documentation of all this, please visit: https://2019.igem.org/Team:Sheffield/Human_Practices.
Gold
We built and kept adapting our microplate reader based on the feedback we received from potential end-users and customers. For more information, please visit: https://2019.igem.org/Team:Sheffield/Human_Practices.
02 Improve a previous project
Our project is an improvement of the 2017 Sheffield iGEM project called BrightBiotics. The specifics of these improvements are as follows:
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Point 1
The 2017 Sheffield iGEM microplate reader employs 96 sensors and 96 detectors all wired up. The 2017 team, only managed to make a few rows functional and accurate. In addition, rebuilding this was not straightforward. -
Improvement 1
Our microplate reader, uses a single LED sensor and a single photodiode detector which are moved around by a motor, thus making the wiring much simpler to replicate. It also eliminates the need to standardise 96 different readings. -
Point 2
No further space for the addition of extra features. -
Improvement 2
Our device is modular, and its parts can be removed and replaced with alterations. For instance, the 96-well plate platform can be replaced with a revised version that has a shaking mechanism attached. For more details, please visit: https://2019.igem.org/Team:Sheffield/Hardware. -
Point 3
BrightBiotics would send the readings to an online database called ThinkSpeak, where you could download the values from. There is no interaction with the user. It only operates in an on/off fashion. -
Improvement 3
Openlux has its own software that provides the user with a platform to interact with the machine. The user can select wells, create programs, save the data from any device that has a browser.
03 Model your project
We have used CAD in order to create 3D models of our device prior to manufacturing. This elucidated errors in our design would have only been picked up after the construction of the device. For more information, please visit: https://2019.igem.org/Team:Sheffield/Model.
04 Demonstration of Your Work
For more information, please visit: https://2019.igem.org/Team:Sheffield/Demonstrate.