Team:SDSZ China
Team:SDSZ China
ABOUT US
We are SDSZ_China iGEM team. Our team is consisted of 8 passionate high school students from The Experimental High school Attached to Beijing Normal University, 6 of whom will be attending the Giant Jamboree in Boston. All of us have specific interests in synthetic biology and are ardent to use our knowledge, ideas and hard work to bring forth some changes to this world. This is our third year participating in the iGEM competition, and we look forward to continuing for years to come!
ABSTRACT
As one of the most common pollutants of fecal-polluted water, somatic coliphage is widely used in various protocols as the indicator of fecal and viral pollution. Our team designed a biosynthetic system that effectively detects bacteriophages in fecal polluted samples by inserting sequences of firefly luciferase and polyphenol oxidase in pET 28a and pET 30a vectors. After adding appropriate substrates to LB medium, enzymatic reactions occur when cell lysis takes place due to phage attack, and expressed enzymes leak from lysed cells to interact with their according substrates. Fluoresce and observable color change of the enzymatic reactions will then be tested and recorded for identification of the sample’s phage density, and thus the tested sample’s degree of pollution can be indicated. To avoid false negative responses, our team further inserted sequences downstream to express 2-Phenylethanol, emitting scent which acts as expression indicator that will not hinder luminescence detection.
Before you start
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2019 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2019 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples: