iGEM Lab Note
9/3
Transformation of BBa_I0500,BBa_I14032,BBa_I13453 and BBa_J04450.
9/5
Colony PCR and preculture BBa_J04450 and IDT's parts.
9/6
Miniprep and treats BBa_J04450 and parts we get from IDT with restriction enzyme specified.
9/7
Preculture BBa_J04450 and parts from IDT.
9/8
Miniprep BBa_J04450 and IDT's parts.
9/10
DNA purification and gel electrophoresis BBa_J04450 and IDT's parts.
Ligation and Transformation of BBa_J04450 and IDT's parts.
9/11
Preculture BBa_J04450 and IDT's parts.
9/12
Miniprep BBa_J04450 and IDT's parts.
9/13
Restriction enzyme treatments of BBa_J04450 and IDT's parts.
9/16
Miniprep BBa_J04450 and IDT's parts.
9/17
Gel electrophoresis and colony PCR of BBa_J04450 and IDT's parts.
9/18
DNA purification and DNA sequencing of BBa_J04450 and IDT's parts.
9/19
Ligation and Transformation of BBa_J04450 and IDT's parts.
Preculture BBa_J04450 and IDT's parts.
9/20
Miniprep BBa_J04450 and IDT's parts.
And performed transformation after treatment of specified restriction enzyme.
9/21
Colony PCR and Preculture BBa_J04450 and IDT's parts.
9/23
DNA purification and gel electrophoresis of BBa_J04450 and IDT's parts.
9/24
DNA sequencing of BBa_J04450 and IDT's parts.
9/25, 26
Restriction enzyme treatment and gel electrophoresis of BBa_J04450 and IDT's parts.
Preculture BBa_J04450 and IDT's parts.
9/27
DNA purification of BBa_J04450 and IDT's parts.
9/28
From Restriction enzyme treatment to Transformation.
9/30
Colony PCR of BBa_J04450 and IDT's parts.
10/1~7
Ligation, transformation and Colony PCR of BBa_J04450 and IDT's parts
10/9
Ligation and Transformation of BBa_J04450 and IDT's parts.
10/10
Transformation of BBa_J04450 and IDT's parts.
10/11
DNA purification of BBa_J04450 and IDT's parts.
10/12
Precultures of E. coli containing each expression vector of BBa_I0500, BBa_14032 and BBa_I13453. Wiki creation. Judging form creation.
10/13
Subculture those modified E. coli and induction of enzyme by addition of arabinose and IPTG. Wiki creation. Judging form creation.
10/14
Extraction of protein from cultured E. coli. and confirmation by gel electrophoresis.
Precultures of E. coli containing each expression vector of BBa_I0500, BBa_14032 and BBa_I13453.
Wiki creation.
10/15
Subculture of those E. coli and induction of enzyme by addition of arabinose and IPTG.
Wiki creation.
10/16
Extraction of protein from cultured E. coli. and confirmation by gel electrophoresis.
Wiki creation.
10/17
Extraction of protein from cultured E. coli. and confirmation by gel electrophoresis.
Precultures of E. coli containing each expression vector of BBa_I0500, BBa_14032 and BBa_I13453.
Wiki creation.
10/18
Subculture of those E. coli and induction of enzyme by addition of arabinose and IPTG.
Wiki creation.
10/19
Extraction of protein from cultured E. coli. and confirmation by gel electrophoresis.
Wiki creation.
10/20
Wiki creation.