Team:FAU Erlangen/Composite Part
Composite Parts
We, as the iGEM team FAU_Erlangen aim to create bispecific antibodies. Therefore we created six composite parts for the expression in eukaryotic and prokaryotic cells. The parts include the sequence for a complete bispecific antibody and two constructs with either SpyTag or SpyCatcher sequence attached to build a comparable bispecific antibody, but with the possibility to create a modular system for bispecific antibodies supported by our simulations prior to construct design.
scFv targeting GPA33 connected with a SpyCatcher, which was codon optimized for expression in CHO-S
Bispecific antibody consisting of two scFv targeting GPA33 and CD3, which was codon optimized for expression in CHO-S
scFv targeting CD3 connected with a SpyTag, which was codon optimized for expression in CHO-S
Bispecific antibody consisting of two scFv targeting GPA33 and CD3, which was codon optimized for bacterial expression
scFv targeting GPA33 connected with a SpyCatcher, which was codon optimized for bacterial expression
scFv targeting CD3 connected with a SpyTag, which was codon optimized for bacterial expression