Team:Chalmers-Gothenburg/Experiments
Experiments
The experimental workflow used during our iGEM project involved many types of experiments. These included, but were not limited to: PCR, Gibson Assembly, gene editing with CRISPR/Cas9 and Western blot. To get a detailed overview of the workflow and the purpose behind each experiment, please refer to the experimental design described on the design page. All of the relevant experimental protocols used during our iGEM project can be found below.
PCR Protocols
Thermo Scientific DreamTaq DNA Polymerase protocol
Thermo Scientific Phusion High-Fidelity DNA Polymerase protocol
PrimeStar HS DNA Polymerase protocol
Purification and Plasmid Extraction Protocols
Thermo Scientific GeneJET PCR Purification Kit protocol
Thermo Scientific GeneJET Gel Extraction Kit protocol
Digestion and Assembly Protocols
Thermo Scientific FastDigest Restriction Enzymes protocol
Thermo Scientific GeneJET Plasmid Miniprep Kit protocol
Transformation Protocols
E. coli transformation protocol
S. cerevisiae transformation protocol
Measurement Protocols
Miscellaneous Protocols
Protocols for making buffers and solutions
EasyClone Markerfree User Manual
Further Reading
Check out the rest of our lab-related work by clicking on any of the images below! Go through all of the 4 months of lab work documented in Notebook, read up on the details of the different Parts used, or go straight for the actual Results of all our hard work. Under Contribution we have documented how we contributed to iGEM by further characterizing already existing BioBricks, while Safety will take you to the details of how we made sure to keep ourselves and others safe in the lab.
