Team:BIT/Collaborations

Collaborations

In July this year, we cooperated with iGEMers from Zhejiang University of Technology.

The final product of our project this year is aimed to detect substances in the environment that can cause oxidative damage. To detect specific pollutants in the environment, we first need to understand the current situation of environmental pollution. This is very important for our project.

The iGEMers from Zhejiang University of Technology provide us with a statistical table of environmental pollution data in China. This table is from the first national census of pollution sources. A total of 5.926 million people were surveyed, including 1.576 million industrial sources, 2.899 million agricultural sources, 1.446 million household sources, and 4,790 centralized pollution control facilities.

The report gives an overall picture of the number and distribution of pollution sources in China. The situation is described in detail from three aspects: industrial pollution source, agricultural pollution source and domestic pollution source.

Through these data, we fully understand the current situation of environmental pollution sources in China. So We know better what the results of the project should be.

We are very grateful to them in terms of background and ideas to help our project! Details of the table are shown in annex I.
Annex I

Here is the link to their wiki:
https://2019.igem.org/Team:ZJUT-China/Collaborations

July 10th，cooperation between BIT-China and BIT kicked off. We changed our previous kit Plates in their lab for our subsequent experiments . We provided them with plasmid containing BBa_C0061 and BBa_C0062. And they provided us with plasmid containing BBa_E0840 and we used it to construct our plasmid. Here is the link to their wiki.

In July 18th ,we provided them with the E.coli strain containing BBa_F2620 ,and they used it to build their quorum sensing ststem. It helped them finish their experiment plan faster.

our team leader and BIT-China team leader Yu Fanchen

In October 3rd and 7th , we shared them the right of using microplate reader twice and helped them measure and verify the experimental results of quorum sensing system and T7 orthogonal expression system.

Here is the link to their wiki:
https://2019.igem.org/Team:BIT-China/Collaborations

We completed a survey with iGEMers from Taiwan about people’s understanding of synthetic biology. We developed and distributed two different questionnaires for the common purpose. We got the data from the mainland and Taiwan respectively. After comparing the data and coming to a conclusion, we know how to promote synthetic biology better in the future.

In the survey,there are 28 questionnaires collected in Taiwan,at the same time, we have 82 samples in the mainland

According to the collected data, we found the sample frame that most of the subjects in the two places are young people under 30 years old. When it comes to academic qualifications, the mainland samples are highly concentrated on undergraduate level, while Taiwan samples are more evenly distributed in high school and university.

By comparing the sample data of Taiwan and the mainland, we can know that in Taiwan, people mainly know the relative terms of synthetic biology through the Internet media, reaching 50% of all. However, people in the mainland usually learn via the Internet and professional studying. In both Taiwan and China, 25% or more subjects have no access to get such kind of information, which means that there are still a highly number of people having no conception about synthetic biology.

And most people in both agree that the pros outweigh the cons when referring to the pros and cons of synthetic biology. Especially in the mainland survey, almost 34% people hold a moderate attitude toward this problem than just think the disadvantages are big.

It is worth noting that Taiwan has a high proportion of daily exposure to synthetic biological applications or products, up to 64.2%. In contrast, more than three-quarters of the mainland have no experience with such applications or products in their lives. It implies that people in Chinese mainland lack of awareness of synthetic biology.

We hope that more and more people will know or understand synthetic biology! Details of the questionnaires are shown in annex Ⅱ and Ⅲ.
Annex II
Annex III

Here is the link to their wiki:
https://2019.igem.org/Team:CCU_Taiwan/Collaborations

We cooperated with iGEMers from Nanjing Agricultural University this year.

Initially we wanted to do the “Characterization / Contribution” part of the bronze standard. Based on the experimental conditions, we chose to calibrate the flow cytometer. But we don’t have the Rainbow Calibration Kit which contains the required material, and it is very expensive for us to use it only once.

Fortunately, We got in touch with iGEMers from NAU, and they provided us with SPHEROTM Rainbow Calibration Kit for standardization in flow cytometry experiments. Thanks very much for their generous help!

It’s our honor to help them with their project. We helped them measure the bacterial growth curve of S. marcescens involved in their project to help they have a more deep understanding of the physiological characteristics of their bacteria.

What a delightful cooperation it is! Details of the table are shown in Annex IV.
Annex IV

Here is the link to their wiki:
https://2019.igem.org/Team:NAU-CHINA