Team:SDSZ China/Att
Team:SDSZ China
We are SDSZ_China iGEM team. Our team is consisted of 8 passionate high school students from The Experimental High School Attached to Beijing Normal University, 6 of whom will be attending the Giant Jamboree in Boston. All of us have specific interests in synthetic biology and are ardent to use our knowledge, ideas and hard work to bring forth some changes to this world. This is our fourth year participating in the iGEM competition, and we look forward to continuing for years to come!
Yuhe Shen
As the team leader, Yuhe is in charge of every aspects of the team. She came up with the creative idea of improving bluephage and diligently designed the project. Because our high school’s bio lab is not open to iGEM teams anymore beginning this year, she strived to find a lab in China Agricultural University to conduct our experiment. She also spent months in experiments while contemplating about ways to continue improving our project.
Yufei Liu
As the team leader, Yuhe is in charge of every aspects of the team. She came up with the creative idea of improving bluephage and diligently designed the project. Because our high school’s bio lab is not open to iGEM teams anymore beginning this year, she strived to find a lab in China Agricultural University to conduct our experiment. She also spent months in experiments while contemplating about ways to continue improving our project.
Yufei Liu
Having worked on molecular biology researches, Yufei contributes a lot to our experiment. She was in charge of designing the specific parts of our experiment, like primer and plasmid design, and also spent months in the lab. She is also in charge of drawing the illustration of our reaction. Moreover, when returning to her hometown, Xiaobeihe, she investigated the local sewage treatment plant, bringing back information that helps in improving the project.
Huarui Liang
Huarui is adept in biology, computer science and math. Besides participating dedicatedly in experiments, he was also in charge of coding our team wiki and building valid mathematic model for our part. He also organized the visit to the domestic wastewater treatment plant and got valid suggestions on improving the project, which constitutes an important part of our human practice.
Yisheng Liu
Yisheng is gifted in mathematics, physics and biology. He adapts his knowledges into the lab well, and treated all the tasks assigned to him, both inside and outside of the lab, with seriousness. He also participated actively in human practices.
Mukun Liu
Mukun is fluent in English, which contributed a lot to our team’s presentations and writings. She is also ardent in learning about biology techniques. She contributed a lot to our experiments, participated actively in human practice, and would always finish her tasks with efficiency and high quality.
Siqi Zhou
Siqi is adept at reading literatures. Thanks to her help, we are able to review and discuss more information from previous researches, which helps a lot when developing the PPO track.
Yuqiao Hu
As the first member to join our iGEM team, Yuqiao worked hard to finding a lab. Proficient in computer science and also gifted in art, she plays an important role in coding team wiki, designing our team logo and poster, and building model for our hardware.
Tianyang Han
Tianyang, as a fluent English speaker, contributed a lot to our Beijing iGEM meetup. With her love towards biology, she also sacrificed many of her time to the team’s program design and experiments.
Acknowledgement:
Team SDSZ_China is thankful to
- WeiFang
For giving us useful instructions on strictly following protocols during experiments and offering us resources such as luciferase vector addgene-plasmid-37851-sequence-180888
- Jianhan Hu
For instructing our team through all the processes before experiments and providing valuable experience on team management and project design.
- Xuewei Yin
For providing us molecular biology lab in China Agricultural University and helping with reagents purchase.
- Zhangqi Shen and Lu Liu
For offering us devices on electroporation and resources such as plasmid vector pET-30a(+) and coliphages of different strains.
- Luying He
For offering us valuable advices on project optimization (possibly adding 2-PE sequences to our plasmid) and reaching out to deliver FJNU-China’s part in 2018 to our team. Although the delivery of plasmids was not successful in the end, we were thankful for her kind advices and generous will to help.
v- Xuan Shang
For allowing us to conduct some part of our experiments in Fei Dou’s lab in Beijing Normal University and kindly offering us 10ul of in-fusion buffer and 5ul of enzyme EcoRV.
- China Agricultural University
- Beijing Normal University
- The Experimental High School Attached to Beijing Normal University
Our references:
1. LeChevallier, M., Kwok-Keung, A., 2004. WHO | Water treatment and pathogen control: Process efficiency in achieving safe drinking water. WHO drinking water quality. World Health Organization, London, UK. 2. U.S. EPA, Summary of the Clean Water Act. Washington D.C. https://www.epa.gov/laws-regulations/summary-clean-water-act 3. U.S. EPA, Summary of the Safe Drinking Water Act. Washington D.C. https://www.epa.gov/laws-regulations/summary-safe-drinking-water-act 4. Ngoc Han Tran a, Karina Yew-Hoong Gin b,?, Huu Hao Ngo c,?, 2015. Fecal pollution source tracking toolbox for identification, evaluation and characterization of fecal contamination in receiving urban surface waters and groundwater. Science of the Total Environment 538 (2015) 38®C57 5. D.H.Schuettpelz, 1969. Fecal and total coliform tests in water quality evaluation. Department of Natural Research, Research Report 42. Madison, Wis. 6. USEPA, 2015. Review of Coliphages as Possible Indicators of Fecal Contamination for Ambient Water Quality. EPA Office of Water (820-R-15-098). 7. M. Muniesa, E. Ballest, L. Imamovic, M. Pascual-Benito, D. Toribio-Avedillo, F. Lucena, A.R. Blanch, J. Jofre. Bluephage: A rapid method for the detection of somatic coliphages used as indicators of fecal pollution in water. Water Res. 2018 Jan 1;128:10-19. doi: 10.1016/j.watres.2017.10.030. Epub 2017 Oct 16. 8. Josue Flores-Kim and Andrew J. Darwin. The Phage Shock Protein Response. Annu. Rev. Microbiol. 2016. 70:83-101 9. Goran Jovanovic, Jonathan Dworkin, and Peter Model. Autogenous control of PspF, a Constitutively Active Enhancer-Binding Protein of Escherichia coli. Journal of Bacteriology, Aug. 1997, p. 5232-5237