Team:uOttawa/Software

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Software

With the hope to help the students of the team with the design of primer, I tried to develop a software to do primer design. There is still work to do on it, but I think what I did could help some others students with the goal to write a software like this to ! You can click on this button to get all the code I wrote in Python :

Python

And on this one to get the code in R:

R

So, in this page, I would like to present this software. The chapter 0 explains how to use it, the chapter 1 and 2 how it was programmed and the chapter 3, what would be to improve next.

0 - How to use the programm

Copy-paste the whole python code in repl.it ; Replace the string contained in the variable seqDNA (line 807) with the total sequence of DNA that is going to enter in the PCR; Replace the string contained in the variable target (line 809) with the part of the total sequence of DNA you want to duplicate (and so, the primers must be around it) Run the program Wait for a dozen of minutes There should appear a list of suggested primers, with the one at the bottom being the most recommended