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Notebook_XHD-WS-Wuhan-B

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2019/7/16

Content of the experiment: Colony PCR

Raw material: LB medium

chloramphenicol

Colony of bacteria

Steps:

1. Take 2 mL LB culture medium and add it into two 2 mL EP tubes.

2. 2 uL chloramphenicol was added to the EP tubes.

3. Blend slightly.

4. Take 15 1.5mL EP tubes.

5. 200 uL medium containing anti-LB was added into EP tube.

6. Five colonies were taken from each Petri dish and added into the corresponding EP tube.

7. Shake bacteria on shaking bed at 37 C for 2 hours.

Result:

The turbidity of the bacterial liquid indicates that the bacterial body has successfully reproduced and the concentration of the bacterial liquid is high.

Tips:

1. We made three strains, s, W and m.

2. Chloramphenicol is a screening agent, which can remove impurities.

2019/7/18

Content of the experiment: Measuring OD value

Raw materials:

Eon enzyme labelling instrument

Diluted S4 W1 M5

S4 W1 M5 stock solution

S3 M2 W2 stock solution

Steps: (For detailed steps of multiple dilution, see the relevant documents) 1. Two 1.groups of diluted S4 W1 M5 and 100ul each were added to the enzyme label plate.

2. Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see figure for data).

The measured OD value is about 0.05, not between the most suitable OD value (0.4-0.6), so the raw liquor with higher concentration is taken to measure OD value again.

3. The raw liquor of S4 W1 M5 and 100ul of each group were added to the enzyme label plate.

4. Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see figure for data).

The measured OD value is more than 0.6, slightly higher than the most suitable OD value (0.4-0.6). Therefore, W 1 with the smallest difference between the two values (the smallest error) is selected as the ratio dilution. OD value is measured again, and the functional relationship between OD and concentration is established to find the appropriate dilution multiple.

5. Dilute the ratio of raw solution of W1 to 1, 2, 4 and 8 times of concentration and add two groups of 100ul to the enzyme label plate.

6. Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see the yellow part of the icon for the data).

The function curve of OD value with respect to concentration is established with Excel table (see figure).

The OD value is 0.5926 when the OD value is 0.4 and 0.635 when the concentration multiple is 1, so the dilution multiple should be 1/0.5926 = 1.6874 for the original solution of W1. (principle of calculation: concentration = volume / volume, concentration is inversely proportional to volume, and dilution multiple is equal to volume multiplier, so the concentration ratio is inversely proportional to dilution multiple, and the concentration multiple is the reciprocal of dilution multiple).

Because the raw liquor of S4, W1 and M5 is not much surplus, it is decided to take S3, W2 and M2, and to measure the required dilution multiples for use in subsequent inspection.

7. Two groups of 100ul each of S3 W2 M2 bacterial solution were added to the enzyme label plate.

8.Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see figure for data).

The measured OD value is about 0.7, slightly higher than the most suitable OD value (0.4-0.6), so the average values of S3, W2 and M2 are taken into the function system.

9. Calculate the required dilution multiples:

Result:

According to the calculation results, it can be seen that the dilution factor is about 2 times.

2019/7/19

Content of the experiment: Observation of bacteria under ultraviolet radiation

Raw materials:

Ultraviolet irradiator

Resuspended centrifuged bacteria

Steps:

The groups S, W and M were irradiated by ultraviolet instrument and photographed. Principle:

When mCherry is repressed by lead, it expresses fluorescent protein and fluoresces under ultraviolet irradiation.

Result:

Group S: Compared with the control group, S ^ 10-4 showed darker colour, while the other bacteria in lead concentration showed no obvious fluorescence.

Group M: Compared with the control group, M ^ 10-4 showed darker colour, while the other bacteria in lead concentration showed no obvious fluorescence.

Group W: Compared with the control group, the bacteria of W ^ 10-4 showed a deeper color, and the bacteria with lead concentration of 10-7 had obvious red fluorescence, while the bacteria with other lead concentration had no obvious fluorescence.

Abnormal situation and analysis:

1. W10 ^ - 7 is the only visible red fluorescence in all bacterial fluids.

- It may be that the high concentration of lead acetate (toxic) leads to the death of other strains, or that mCherry cannot express (emits red fluorescence).

- It may be that in S and M, the concentration of PbrR is too high, or the repression ability is too strong, which results in the absence of mCherry expression in bacterial solution.

- It may be that the system of W10 ^ - 7 has unexpected abnormalities (mutation of strain (base deletion) or changes in expression.

2. S ^ 10-4, M ^ 10-4, W ^ 10-4 are darker and some are black.

- It may be that high lead concentration leads to cell death.

2019/7/23

Content of the experiment: Enzyme linkage

Raw materials:

TaKaRa: DNA Ligation Kit Ver.2.1

Solution I

Enzymatic digestion products of low-copy skeleton

Enzymatic Digestion Products of Synthetic Fragments of pPbrR-S4 and mCherry Enzymatic products of the synthetic fragments of pbrr-m5 and mcurry

Enzymatic Digestion Products of Synthetic Fragments of pPbrR-W1 and mCherry DdH2O

Steps:

1. Take 4 PCR tubes

2. Solution I with 4ul each

3. Low-copy skeletons with 1 UL each

4. The synthetic fragments of pPbrR-S4 and mCherry, pPbrR-M5 and mCherry, pPbrR-W1 and mCherry were added respectively.

5. Add 1 UL of ddH_2O each.

6. Set the temperature of 16 degrees Celsius in the PCR for 1 hour. Tips: After the connection, it needs to be instantly transformed into a receptive state.

2019/7/23

Content of the experiment: Axygene

Raw materials:

Agarose Gel Containing Purpose DNA

Buffer DE-A 400 UL

Buffer DE-B 200 ugl

Buffer W1 500 ugl

Buffer W2 700 mu l*2 = 1400 Mu L

Eluent 25 UL

Instruments:

blb

2.0 ml centrifugal tube

1.5 ml centrifugal tube

Water bath

DNA preparation tube

Centrifuge

Refrigerator

Steps:

1. Cut agarose gel containing target DNA under purple lamp and place it in 2.0 ml centrifugal tube. -

2. Add 400 ml Buffer DE-A, mix evenly, then heat in 75 C water bath, mix in intervals (every 2-3 minutes) until gel block melts completely (5 minutes).

3. Add 200 ml Buffer DE-B and mix evenly.

4. Absorbing the mixture of the above steps and transferring it to the DNA preparation tube (placed in 2 ml centrifugal tube), centrifuging for 1 minute at 12 000*g, discarding the filtrate

5. Put the prepared tube back into centrifugal tube, add 500 ml buffer W1, 12 000*g centrifugal for 30 seconds, discard filtrate

6. he prepared tube was placed back into the centrifugal tube, adding 700 ml buffer W2, 12 000*g centrifugal for 30 seconds, discarding the filtrate. Wash again in the same way with 700 ml buffer W2 and centrifuge for 1 minute at 12000*g.

7. Place the tube in a 2 ml centrifugal tube and centrifuge for 1 min at 12000*g. - The preparation tube was placed in a clean 1.5 ml centrifugal tube. 25 ml Eluent was added in the center of the DNA preparation membrane. The tube was placed at room temperature for 1 minute and centrifuged at 12 000*g for 1 minute to elute DNA.

8. The recovered DNA samples were stored at 4 C for short-term storage for subsequent experiments.

Tips

- Waterbaths should be intermittently checked for gel melting

- When using centrifuges, attention should be paid to the symmetrical placement of centrifugal tubes. If there are odd number of centrifugal tubes, balance of the total mass of the symmetrical centrifugal tubes (i.e. the sum of the mass of the centrifugal tubes and the mass of the substances therein) should be used.

2019/7/23

Content of the experiment: Purification and validation

Steps:

1. Place the plywood in the electrophoresis tank and add the electrophoresis buffer.

2. Adding 4 UL marker to an end-point sample hole

3. The purified products were added to 1 UL Loading Buffer with 2 UL each.

4. The purified products were added into the dot-like pore in the order of vector, S, M and W (when the black band was near the observer) from left to right.

5. Adding 4 ml marker to the end point sample hole

6. Open the electrophoresis apparatus and adjust the voltage to 90V to make the nucleic acid sample swim towards the positive pole (note that the sample hole is at the negative end of the electrophoresis tank). Time: 40 minutes

After the electrophoresis was completed, the power supply was cut off, the gel was taken out, the electrophoresis results were observed on the ultraviolet transmitter and photographic records were taken.

Tips:

1. Set the alarm clock to remind you to check the progress of running glue at intervals and stop running glue when you reach the total progress of 2/3 from the beginning.

Vector, M, S all run out of the bright band

W did not run out of the bright band.

Anomaly analysis:

It may be that the concentration of W bacterial solution is too low, which leads to the inadequate appearance of bright bands in the proposed plasmid concentration.

2019/7/23

Experiments: Enzymatic digestion of rubbing gum

Steps:

7. Place the plywood in the electrophoresis tank and add the electrophoresis buffer.

8. Adding 4 UL marker to the end-point sample hole

9. Put 50 ml of enzymatic digestion products from left to right in the order of Vector, S, M, W (when the black band is close to the observer) and then add them into the dot-like pore.

10. Add 4 ml marker to the end point sample hole

11. Open the electrophoresis apparatus and adjust the voltage to 90V to make the nucleic acid sample swim towards the positive pole (note that the sample hole is at the negative end of the electrophoresis tank).

Time: 70 minutes

- After the electrophoresis was completed, the power supply was cut off, the gel was taken out, the electrophoresis results were observed on the ultraviolet transmitter and photographic records were taken.

Tips:

2. Set the alarm clock to remind you to check the progress of running glue at intervals and stop running glue when the target strip reaches about 1/2 of the total length of the glue.

S, M and W run out of 3000, 2000 and 1500 BP bands respectively.

Vector runs out a bright band of about 2000 bp.

Analysis of abnormal results:

S, M and W run out of the bright band of about 3000bp

1. It may be that the short cleavage time resulted in the failure of some S, M and W plasmids to be cut off.

2019/7/23

Plasmid extraction:

Raw materials:

1 Bottle of Bacterial Solution: W1

Test tube, liquid gun, centrifuge, oscillator;

Kit: Hlingene High Purity Plasmid Small Quantity Rapid Extraction Kit, Shanghai Huiling Biotechnology Co., Ltd.

Buffer (Protecting DNA, Resuspending DNA), S2 (Alkaline lysis), S3 (Acidic Neutralizer + Protein Precipitation Promotion), washer 1, Eluent.

Step report:

Collection centrifugation: 1.5 ml centrifugal tube with 1.3 ml bacterial solution, 12 000 rpm centrifugation for 30 seconds, discarding supernatant

Repeat the above process 4 times and add 5.2 ml bacterial solution.

Resuspension: adding 500 UL buffer S1, the shock is sufficient to completely free of small bacteria;

Move all solutions to 2ml EP tube

Pyrolysis: Add 500 UL of S 2, gently inverted about 10 times, so that the solution becomes clear; Neutralization: adding 700ul S3, gently inverted about 10 times, white flocculent objects appeared, centrifuged at 13000 rpm for 15 minutes;

Collection: absorb supernatant (do not touch white precipitation) 600ul, transfer it into the adsorption column of the collection tube, centrifuge at 12000 rpm for 30 seconds, and pour out the waste liquid; repeat the above action once

Cleaning: add 600ul WB lotion to the adsorption column, centrifuge at 12000rpm for 30s, discard the filtrate, and repeat it.

Air centrifugation: 12000 RPM centrifugation for 30 seconds;

The column was replaced by a new 2ml centrifugal tube.

Elution of the heart: add 65ul (50-100ul) eluent, stand for 2 minutes, centrifuge for 1 minute at 12000 rpm.

Tips:

1. New collecting tubes need to be replaced from centrifugation to heart washing. 2. When adding bacterial liquid, it is necessary to see the mark clearly, and the pipes need to be corresponded.

3. Use up the solution and remember to cover it.

4. The eluent needs to be preheated in advance.

Concentration 132.5

Abnormal:

1. The centrifuged S and M are reddish, the reason is unknown.

2019/7/24

Experiment content: enzyme cutting

Pbrr-s 50ul system：

10x green buffer 5ul

EcoRI 2ul

PstI 2ul

Ppbrr-s 14ul

DdH2O 27ul

Pbrr-m 50ul system：

10x green buffer 5ul

EcoRI 2ul

PstI 2ul

Ppbrr-m 22ul

DdH2O 19ul

Pbrr-w 50ul system：

10x green buffer 5ul

EcoRI 2ul

PstI 2ul

Ppbrr-w 41ul (41.9ul should have been added)

DdH2O 0ul

Low copy skeleton vector 50ul system：

10x green buffer 5ul

EcoRI 2ul

PstI 2ul

Skeleton plasmid 14ul

DdH2O 27ul

step

1. Match the above systems

2. Put in the PCR instrument, 37 ℃, 45 minutes

Tips:

1. Enzyme should be added first

2019/7/24

Experiment content: PCR

20ul system

1. Taq mix (2x) 10ul

2. Primer (F1) 1ul

3. Primer (R2) 1ul

4. Template (bacterial solution) 1ul

5. DdH2O 7ul

PCR process:

Set 20ul

Start:

94 ℃ 5min

Cycle step (34 times):

94 ℃ 30s

55 ℃ 30s

72 ℃ 1min 40s

End:

72 ℃ 5min

Finally placed at 4 ℃

Raw materials:

Taq 2x Mix

Up primer F1

Lower primer R2

bacterial fluid

DdH2O

Steps:

1. Mark 16 PCR tubes

2. Add 10ul Taq enzyme solution to each PCR tube.

3. Add 7ul ddH2O into each PCR tube

4. Add 1ul F1 primer to each PCR tube

5. Add 1ul R2 primer to each PCR tube

6. Add 1ul s bacteria solution to 5 tubes, 1ul m bacteria solution to 5 tubes, 1ul w bacteria solution to 5 tubes, and 1ul ddH2O to the remaining tube.

7. Put all configuration products into PCR machine

8. Set up PCR instrument:

Set volume to 20ul

The pre denaturation temperature is 94 ℃, 5 minutes

Repeat the following three steps 34 times

Denaturation temperature 94 ℃, 30 seconds

Annealing temperature 55 ℃, 30 seconds

Extension temperature 72 ℃, 1 minute 40 seconds

Set to stay at 72 ℃ for 5 minutes

Last stop at 4 ℃

Tips:

1. When matching the PCR system, it is necessary to confirm whether the measurement and proportion are accurate. If bubbles appear, it is necessary to take them again.

2. When adding bacterial solution to PCR tube, it is necessary to confirm whether it corresponds to

3. Pay attention to the accuracy of input value when setting up PCR instrument

1. S1 did not run out of bright band, S2, S3, S4, S5 all ran out of bright band.

2. M3 and M4 did not run out of bright band, M1, M2 and M5 all ran out of bright band.

3. W ran out of the bright band.

(Abnormal) There is a dark band in the negative control, the reason is unknown.

2019/7/24

Experiment content: EC20 2 SMTA 2

raw material

LB liquid medium

EC20 2 bacteria solution

SmtA 2 bacteria solution

Experimental steps

EC20 2

-20 ml LB medium was added into a large glass bottle.

-Add 40 μ L acellin to the glass bottle, and mark ec202 on the bottle body.

-Add 80 μ L ec202 bacterial solution into the glass bottle.

SmtA

-5 ml of LB medium was added to the other four glass bottles, and SMTA 2 was labeled on the bottle body.

-Add 10 μ l of acellin into the glass bottle.

-Add 20 UL of SMTA 2 solution to the glass bottle.

-Put all glass bottles into a 37 ℃ shaker for cultivation

2019/7/24

Experiment content: amplification of SL ml WL

raw material

LB liquid medium

SL bacteria solution

ML bacteria solution

WL bacteria solution

Experimental steps

-4 ml LB medium was added into 15 glass bottles.

-Add 8ul acellin to 15 glass bottles.

-Add 10 μ l of bacteria solution containing s into five glass bottles and mark SL on the bottle body

-Add 10 μ l of bacteria solution containing m into the other five glass bottles and mark ml on the bottle body

-Add 10 μ l of W bacteria solution into the last five glass bottles, and mark WL on the bottle body.

-Put all glass bottles into a 37 ℃ shaker for cultivation

2019/7/25

Experiment content: amplified bacterial solution S2 M1 W3

raw material

LB liquid medium

S2 bacteria solution

M1 bacteria solution

W3 bacteria solution

Ampicillin

Experimental steps

-10 ml LB medium was added into three glass bottles.

-Add 30ul of acellin into 3 glass bottles respectively.

-Add 2ml of S2 bacteria solution into a glass bottle, and mark S2 l 7.25 on the bottle body.

-Add 2ml of M1 bacteria solution into another glass bottle, and mark M1 l 7.25 on the bottle body.

-Add 2ml of W3 bacteria solution into the last glass bottle, and mark w3l 7.25 on the bottle body.

-Put all glass bottles into a 37 ℃ shaker for cultivation

2019/7/25

Experiment content: enzyme cutting and glue releasing

Steps:

-Put the rubber plate into the electrophoresis tank and add the electrophoresis buffer.

- Add 4 μ l marker into the sample hole at one end

-Add 10 μ l enzyme products from left to right in the order of W5, W3, W4, M2, M1, S5, S4, S2 (when the black strip is close to the observer) into the sample hole in turn.

-Add 4 μ l marker to the end sample hole

- Turn on the electrophoresis instrument, adjust the voltage to 90V, and make the nucleic acid sample swim towards the positive pole (note that the sample hole is at the negative end of the electrophoresis tank). Time: 70 minutes

-After electrophoresis, the power is cut off, the gel is removed, and the electrophoresis results are recorded on the ultraviolet transmittance instrument and recorded.

Tips:

3. Set alarm clock to remind, check the glue running progress according to the interval, and stop the glue running when the target strip reaches about 1 / 2 of the total glue length.

S, m and w all run out of two bright bands

One is about 2000 BP and one is about 1500 bp.

In line with the ideal situation.

2019/7/25

Experiment content: enzyme cutting

20ul system of S (S2 S4 S5), m (M1 m2), w (W3 W4 W5) plasmids

10x green buffer 2ul

EcoRI 1ul

PstI 1ul

DNA 5ul

DdH2O 11UL

step

-Match the above systems

2019/7/25

Experiment content: Plasmid Extraction:

Raw materials:

8 bottles of bacterial solution: S2, S4, S5 M1, M2 W3, W4, W5

Tube, liquid gun, centrifuge, shaker;

Kit: Shanghai Huiling Biotechnology Co., Ltd. hlingene high purity plasmid small quantity rapid extraction kit

Buffer, S2, S3, washer1, eluent.

Step report:

Collection and centrifugation: add 1.3ml bacterial solution into 2ml centrifuge tube, centrifugate at 12000rpm for 30s, and discard the supernatant.

Repeat the above process for three times, and add 3.9ml bacterial solution in total. Heavy suspension: add 250ul of buffer S1, shake fully until there is no small bacteria block;

Cracking: add 250ul S2, turn it upside down gently for about 10 times to make the solution clear;

Neutralization: add 350 UL S3, turn it upside down gently for about 10 times, white floccules appear, centrifugation at 13000 rpm for 10 minutes;

Collection: take 600ul of supernatant (do not touch the white precipitate), transfer it into the adsorption column of the collection tube, centrifugate it at 12000rpm for 30s, and pour out the waste liquid;

Washing: add 600ul WB washing solution to the adsorption column, centrifugate at 12000rpm for 30s, and discard the filtrate; repeat once

Air centrifugation: 13000rpm centrifugation for 2min;

Replace the column with a new 2ml centrifuge tube.

Elution centrifugation: add 65ul (50-100ul) eluent, stand for 2min, centrifugation at 12000rpm for 1min.

Tips:

-A new collecting tube is needed during the process from air centrifugation to elution centrifugation.

-When adding the bacterial solution, it is necessary to see the mark clearly, and the added pipes need to be corresponding.

-Remember to cover the used solution.

-The eluate needs to be preheated in advance.

According to 260 / 280 (purity) value

We finally selected S2, M1 and W3 for sequence detection.

2019/7/29

Experiment content: amplification of bacterial solution S4 M2 W5

raw material

LB liquid medium

S4 bacteria solution

M2 bacteria solution

W5 bacteria solution

Ampicillin

Experimental steps

-4 ml LB medium was added into 3 plastic tubes.

-8 UL acellin was added into 3 plastic tubes respectively.

-Add 20ul of S4 bacterial solution into a plastic tube and mark the bottle with S4 l 7.28.

-Add 20ul of M2 bacteria solution into another plastic tube, and mark M2 l 7.28 on the bottle body.

-Add 20ul of W5 bacteria solution into the last plastic tube, and mark W5 l 7.28 on the bottle body.

-Put all plastic tubes into a 37 ℃ shaker for cultivation

July 30, 31, 2019

Experiment content: measuring the growth curve of bacteria

Raw materials:

LB medium

Ampicillin

S4L bacteria solution

M2L bacteria solution

W2L bacteria solution

Lead acetate solution with multiple dilution

Steps:

1. Add 5ml LB medium into 6 plastic tubes respectively

2. Add 10ul acellin into these plastic pipes respectively.

3. Add 50ul s4l bacterial solution into these plastic tubes respectively.

4. In the first tube, add 5ul 10 ^ 1 lead acetate solution with the standard of s4l 10 ^ 1 7.30.

5. Add 5ul 10 ^ 2 lead acetate solution in the second tube, and the standard is s4l 10 ^ 2 7.30.

6. In the third tube, add 5ul 10 ^ 3 lead acetate solution with the standard of s4l 10 ^ 3 7.30.

7. Add 5ul 10 ^ 4 lead acetate solution in the fourth tube, and the standard is s4l 10 ^ 4 7.30.

8. Add 5ul 10 ^ 5 lead acetate solution in the fifth tube, marked as s4l 10 ^ 5 7.30. 9. No lead acetate solution is added in the sixth tube, and the standard is s4l 0 7.30. 10. Take 400ul s4l 10 ^ 1 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 1 0h 7.30.

11. Take 400ul s4l 10 ^ 2 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 2 0h 7.30.

12. Take 400ul s4l 10 ^ 3 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 3 0h 7.30.

13. Take 400ul s4l 10 ^ 4 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 4 0h 7.30.

14. Take 400ul s4l 10 ^ 5 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 5 0h 7.30.

15. Take 400ul s4l 0 7.30 solution, add it into 1.5ml EP tube, and mark it as s00h 7.30. 16. Put all EP pipes into 4 ℃ for storage.

17. Repeat the above steps for m2l and w5l.

18. After that, 400ul of each plastic pipe shall be sampled every 2 hours and put into 1.5ml EP pipe and marked accordingly (for example, the sample with the date of 7.30 on the same day and swaying for 2h in s4l 10 ^ 1 shall be marked with s 10 ^ 1 2H 7.30) until 8 hours.

19. Take three 100ul samples from all samples and add them into the enzyme standard plate to measure their OD value.

According to the OD value, the growth curve of bacteria was drawn under different lead concentration.

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+                                                 <li><a href="http://2019.igem.org/Team:XHD-WS-Wuhan-B/Attributions">Attributions</a> </li>
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+                                            </li>
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-/7/16
-Content of the experiment: Colony PCR
-Raw material: LB medium
-chloramphenicol
-      Colony of bacteria
-Steps:
-. Take 2 mL LB culture medium and add it into two 2 mL EP tubes.
-. 2 uL chloramphenicol was added to the EP tubes.
-. Blend slightly.
-. Take 15 1.5mL EP tubes.
-. 200 uL medium containing anti-LB was added into EP tube.
-. Five colonies were taken from each Petri dish and added into the corresponding EP tube.
-. Shake bacteria on shaking bed at 37 C for 2 hours.
-Result:
-The turbidity of the bacterial liquid indicates that the bacterial body has successfully reproduced and the concentration of the bacterial liquid is high.
-Tips:
-. We made three strains, s, W and m.
-. Chloramphenicol is a screening agent, which can remove impurities.
-/7/18
-Content of the experiment: Measuring OD value
-Raw materials:
-Eon enzyme labelling instrument
-Diluted S4 W1 M5
-S4 W1 M5 stock solution
-S3 M2 W2 stock solution
-Steps: (For detailed steps of multiple dilution, see the relevant documents)          1. Two 1.groups of diluted S4 W1 M5 and 100ul each were added to the enzyme label plate.
-. Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see figure for data).
-The measured OD value is about 0.05, not between the most suitable OD value (0.4-0.6), so the raw liquor with higher concentration is taken to measure OD value again.
-. The raw liquor of S4 W1 M5 and 100ul of each group were added to the enzyme label plate.
-. Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see figure for data).
-The measured OD value is more than 0.6, slightly higher than the most suitable OD value (0.4-0.6). Therefore, W 1 with the smallest difference between the two values (the smallest error) is selected as the ratio dilution. OD value is measured again, and the functional relationship between OD and concentration is established to find the appropriate dilution multiple.
-. Dilute the ratio of raw solution of W1 to 1, 2, 4 and 8 times of concentration and add two groups of 100ul to the enzyme label plate.
-. Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see the yellow part of the icon for the data).
-The function curve of OD value with respect to concentration is established with Excel table (see figure).
-The OD value is 0.5926 when the OD value is 0.4 and 0.635 when the concentration multiple is 1, so the dilution multiple should be 1/0.5926 = 1.6874 for the original solution of W1. (principle of calculation: concentration = volume / volume, concentration is inversely proportional to volume, and dilution multiple is equal to volume multiplier, so the concentration ratio is inversely proportional to dilution multiple, and the concentration multiple is the reciprocal of dilution multiple).
-Because the raw liquor of S4, W1 and M5 is not much surplus, it is decided to take S3, W2 and M2, and to measure the required dilution multiples for use in subsequent inspection.
-. Two groups of 100ul each of S3 W2 M2 bacterial solution were added to the enzyme label plate.
-.Put the enzyme labeling plate into the enzyme labeling instrument to measure its OD value (see figure for data).
-The measured OD value is about 0.7, slightly higher than the most suitable OD value (0.4-0.6), so the average values of S3, W2 and M2 are taken into the function system.
-. Calculate the required dilution multiples:
-Result:
-According to the calculation results, it can be seen that the dilution factor is about 2 times.
-/7/19
-Content of the experiment: Observation of bacteria under ultraviolet radiation
-Raw materials:
-Ultraviolet irradiator
-Resuspended centrifuged bacteria
-Steps:
-The groups S, W and M were irradiated by ultraviolet instrument and photographed.            Principle:
-When mCherry is repressed by lead, it expresses fluorescent protein and fluoresces under ultraviolet irradiation.
-Result:
-Group S: Compared with the control group, S ^ 10-4 showed darker colour, while the other bacteria in lead concentration showed no obvious fluorescence.
-Group M: Compared with the control group, M ^ 10-4 showed darker colour, while the other bacteria in lead concentration showed no obvious fluorescence.
-Group W: Compared with the control group, the bacteria of W ^ 10-4 showed a deeper color, and the bacteria with lead concentration of 10-7 had obvious red fluorescence, while the bacteria with other lead concentration had no obvious fluorescence.
-Abnormal situation and analysis:
-. W10 ^ - 7 is the only visible red fluorescence in all bacterial fluids.
-- It may be that the high concentration of lead acetate (toxic) leads to the death of other strains, or that mCherry cannot express (emits red fluorescence).
-- It may be that in S and M, the concentration of PbrR is too high, or the repression ability is too strong, which results in the absence of mCherry expression in bacterial solution.
-- It may be that the system of W10 ^ - 7 has unexpected abnormalities (mutation of strain (base deletion) or changes in expression.
-. S ^ 10-4, M ^ 10-4, W ^ 10-4 are darker and some are black.
-- It may be that high lead concentration leads to cell death.
-/7/23
-Content of the experiment: Enzyme linkage
-Raw materials:
-TaKaRa: DNA Ligation Kit Ver.2.1
-Solution I
-Enzymatic digestion products of low-copy skeleton
-Enzymatic Digestion Products of Synthetic Fragments of pPbrR-S4 and mCherry            Enzymatic products of the synthetic fragments of pbrr-m5 and mcurry
-Enzymatic Digestion Products of Synthetic Fragments of pPbrR-W1 and mCherry            DdH2O
-Steps:
-. Take 4 PCR tubes
-. Solution I with 4ul each
-. Low-copy skeletons with 1 UL each
-. The synthetic fragments of pPbrR-S4 and mCherry, pPbrR-M5 and mCherry, pPbrR-W1 and mCherry were added respectively.
-. Add 1 UL of ddH_2O each.
-. Set the temperature of 16 degrees Celsius in the PCR for 1 hour.            Tips:            After the connection, it needs to be instantly transformed into a receptive state.
-/7/23
-Content of the experiment: Axygene
-Raw materials:
-Agarose Gel Containing Purpose DNA
-Buffer DE-A 400 UL
-Buffer DE-B 200 ugl
-Buffer W1 500 ugl
-Buffer W2 700 mu l*2 = 1400 Mu L
-Eluent 25 UL
-Instruments:
-blb
-.0 ml centrifugal tube
-.5 ml centrifugal tube
-Water bath
-DNA preparation tube
-Centrifuge
-Refrigerator
-Steps:
-. Cut agarose gel containing target DNA under purple lamp and place it in 2.0 ml centrifugal tube.            -
-. Add 400 ml Buffer DE-A, mix evenly, then heat in 75 C water bath, mix in intervals (every 2-3 minutes) until gel block melts completely (5 minutes).
-. Add 200 ml Buffer DE-B and mix evenly.
-. Absorbing the mixture of the above steps and transferring it to the DNA preparation tube (placed in 2 ml centrifugal tube), centrifuging for 1 minute at 12 000*g, discarding the filtrate
-. Put the prepared tube back into centrifugal tube, add 500 ml buffer W1, 12 000*g centrifugal for 30 seconds, discard filtrate
-. he prepared tube was placed back into the centrifugal tube, adding 700 ml buffer   W2, 12 000*g centrifugal for 30 seconds, discarding the filtrate. Wash again in the same way with 700 ml buffer W2 and centrifuge for 1 minute at 12000*g.
-. Place the tube in a 2 ml centrifugal tube and centrifuge for 1 min at 12000*g.            - The preparation tube was placed in a clean 1.5 ml centrifugal tube. 25 ml Eluent was added in the center of the DNA preparation membrane. The tube was placed at room temperature for 1 minute and centrifuged at 12 000*g for 1 minute to elute DNA.
-. The recovered DNA samples were stored at 4 C for short-term storage for subsequent experiments.
-Tips
-- Waterbaths should be intermittently checked for gel melting
-- When using centrifuges, attention should be paid to the symmetrical placement of centrifugal tubes. If there are odd number of centrifugal tubes, balance of the total mass of the symmetrical centrifugal tubes (i.e. the sum of the mass of the centrifugal tubes and the mass of the substances therein) should be used.
-/7/23
-Content of the experiment: Purification and validation
-Steps:
-. Place the plywood in the electrophoresis tank and add the electrophoresis buffer.
-. Adding 4 UL marker to an end-point sample hole
-. The purified products were added to 1 UL Loading Buffer with 2 UL each.
-. The purified products were added into the dot-like pore in the order of vector, S, M and W (when the black band was near the observer) from left to right.
-. Adding 4 ml marker to the end point sample hole
-. Open the electrophoresis apparatus and adjust the voltage to 90V to make the nucleic acid sample swim towards the positive pole (note that the sample hole is at the negative end of the electrophoresis tank). Time: 40 minutes
-After the electrophoresis was completed, the power supply was cut off, the gel was taken out, the electrophoresis results were observed on the ultraviolet transmitter and photographic records were taken.
-Tips:
-. Set the alarm clock to remind you to check the progress of running glue at intervals and stop running glue when you reach the total progress of 2/3 from the beginning.
-Vector, M, S all run out of the bright band
-W did not run out of the bright band.
-Anomaly analysis:
-It may be that the concentration of W bacterial solution is too low, which leads to the inadequate appearance of bright bands in the proposed plasmid concentration.
-/7/23
-Experiments: Enzymatic digestion of rubbing gum
-Steps:
-. Place the plywood in the electrophoresis tank and add the electrophoresis buffer.
-. Adding 4 UL marker to the end-point sample hole
-. Put 50 ml of enzymatic digestion products from left to right in the order of Vector, S, M, W (when the black band is close to the observer) and then add them into the dot-like pore.
-. Add 4 ml marker to the end point sample hole
-. Open the electrophoresis apparatus and adjust the voltage to 90V to make the nucleic acid sample swim towards the positive pole (note that the sample hole is at the negative end of the electrophoresis tank).
-Time: 70 minutes
-- After the electrophoresis was completed, the power supply was cut off, the gel was taken out, the electrophoresis results were observed on the ultraviolet transmitter and photographic records were taken.
-Tips:
-. Set the alarm clock to remind you to check the progress of running glue at intervals and stop running glue when the target strip reaches about 1/2 of the total length of the glue.
-S, M and W run out of 3000, 2000 and 1500 BP bands respectively.
-Vector runs out a bright band of about 2000 bp.
-Analysis of abnormal results:
-S, M and W run out of the bright band of about 3000bp
-. It may be that the short cleavage time resulted in the failure of some S, M and W plasmids to be cut off.
-/7/23
-Plasmid extraction:
-Raw materials:
-Bottle of Bacterial Solution: W1
-Test tube, liquid gun, centrifuge, oscillator;
-Kit: Hlingene High Purity Plasmid Small Quantity Rapid Extraction Kit, Shanghai Huiling Biotechnology Co., Ltd.
-Buffer (Protecting DNA, Resuspending DNA), S2 (Alkaline lysis), S3 (Acidic Neutralizer + Protein Precipitation Promotion), washer 1, Eluent.
-Step report:
-Collection centrifugation: 1.5 ml centrifugal tube with 1.3 ml bacterial solution, 12 000 rpm centrifugation for 30 seconds, discarding supernatant
-Repeat the above process 4 times and add 5.2 ml bacterial solution.
-Resuspension: adding 500 UL buffer S1, the shock is sufficient to completely free of small bacteria;
-Move all solutions to 2ml EP tube
-Pyrolysis: Add 500 UL of S 2, gently inverted about 10 times, so that the solution becomes clear;            Neutralization: adding 700ul S3, gently inverted about 10 times, white flocculent objects appeared, centrifuged at 13000 rpm for 15 minutes;
-Collection: absorb supernatant (do not touch white precipitation) 600ul, transfer it into the adsorption column of the collection tube, centrifuge at 12000 rpm for 30 seconds, and pour out the waste liquid; repeat the above action once
-Cleaning: add 600ul WB lotion to the adsorption column, centrifuge at 12000rpm for 30s, discard the filtrate, and repeat it.
-Air centrifugation: 12000 RPM centrifugation for 30 seconds;
-The column was replaced by a new 2ml centrifugal tube.
-Elution of the heart: add 65ul (50-100ul) eluent, stand for 2 minutes, centrifuge for 1 minute at 12000 rpm.
-Tips:
-. New collecting tubes need to be replaced from centrifugation to heart washing.            2. When adding bacterial liquid, it is necessary to see the mark clearly, and the pipes need to be corresponded.
-. Use up the solution and remember to cover it.
-. The eluent needs to be preheated in advance.
-Concentration 132.5
-Abnormal:
-. The centrifuged S and M are reddish, the reason is unknown.
-/7/24
-Experiment content: enzyme cutting
-Pbrr-s 50ul system：
-x green buffer 5ul
-EcoRI 2ul
-PstI 2ul
-Ppbrr-s 14ul
-DdH2O 27ul
-Pbrr-m 50ul system：
-x green buffer 5ul
-EcoRI 2ul
-PstI 2ul
-Ppbrr-m 22ul
-DdH2O 19ul
-Pbrr-w 50ul system：
-x green buffer 5ul
-EcoRI 2ul
-PstI 2ul
-Ppbrr-w 41ul (41.9ul should have been added)
-DdH2O 0ul
-Low copy skeleton vector 50ul system：
-x green buffer 5ul
-EcoRI 2ul
-PstI 2ul
-Skeleton plasmid 14ul
-DdH2O 27ul
-step
-. Match the above systems
-. Put in the PCR instrument, 37 ℃, 45 minutes
-Tips:
-. Enzyme should be added first
-/7/24
-Experiment content: PCR
-ul system
-. Taq mix (2x) 10ul
-. Primer (F1) 1ul
-. Primer (R2) 1ul
-. Template (bacterial solution) 1ul
-. DdH2O 7ul
-PCR process:
-Set 20ul
-Start:
-℃ 5min
-Cycle step (34 times):
-℃ 30s
-℃ 30s
-℃ 1min 40s
-End:
-℃ 5min
-Finally placed at 4 ℃
-Raw materials:
-Taq 2x Mix
-Up primer F1
-Lower primer R2
-bacterial fluid
-DdH2O
-Steps:
-. Mark 16 PCR tubes
-. Add 10ul Taq enzyme solution to each PCR tube.
-. Add 7ul ddH2O into each PCR tube
-. Add 1ul F1 primer to each PCR tube
-. Add 1ul R2 primer to each PCR tube
-. Add 1ul s bacteria solution to 5 tubes, 1ul m bacteria solution to 5 tubes, 1ul w bacteria solution to 5 tubes, and 1ul ddH2O to the remaining tube.
-. Put all configuration products into PCR machine
-. Set up PCR instrument:
-Set volume to 20ul
-The pre denaturation temperature is 94 ℃, 5 minutes
-Repeat the following three steps 34 times
-Denaturation temperature 94 ℃, 30 seconds
-Annealing temperature 55 ℃, 30 seconds
-Extension temperature 72 ℃, 1 minute 40 seconds
-Set to stay at 72 ℃ for 5 minutes
-Last stop at 4 ℃
-Tips:
-. When matching the PCR system, it is necessary to confirm whether the measurement and proportion are accurate. If bubbles appear, it is necessary to take them again.
-. When adding bacterial solution to PCR tube, it is necessary to confirm whether it corresponds to
-. Pay attention to the accuracy of input value when setting up PCR instrument
-. S1 did not run out of bright band, S2, S3, S4, S5 all ran out of bright band.
-. M3 and M4 did not run out of bright band, M1, M2 and M5 all ran out of bright band.
-. W ran out of the bright band.
-(Abnormal) There is a dark band in the negative control, the reason is unknown.
-/7/24
-Experiment content: EC20 2 SMTA 2
-raw material
-LB liquid medium
-EC20 2 bacteria solution
-SmtA 2 bacteria solution
-Experimental steps
-EC20 2
--20 ml LB medium was added into a large glass bottle.
--Add 40 μ L acellin to the glass bottle, and mark ec202 on the bottle body.
--Add 80 μ L ec202 bacterial solution into the glass bottle.
-SmtA
--5 ml of LB medium was added to the other four glass bottles, and SMTA 2 was labeled on the bottle body.
--Add 10 μ l of acellin into the glass bottle.
--Add 20 UL of SMTA 2 solution to the glass bottle.
--Put all glass bottles into a 37 ℃ shaker for cultivation
-/7/24
-Experiment content: amplification of SL ml WL
-raw material
-LB liquid medium
-SL bacteria solution
-ML bacteria solution
-WL bacteria solution
-Experimental steps
--4 ml LB medium was added into 15 glass bottles.
--Add 8ul acellin to 15 glass bottles.
--Add 10 μ l of bacteria solution containing s into five glass bottles and mark SL on the bottle body
--Add 10 μ l of bacteria solution containing m into the other five glass bottles and mark ml on the bottle body
--Add 10 μ l of W bacteria solution into the last five glass bottles, and mark WL on the bottle body.
--Put all glass bottles into a 37 ℃ shaker for cultivation
-/7/25
-Experiment content: amplified bacterial solution S2 M1 W3
-raw material
-LB liquid medium
-S2 bacteria solution
-M1 bacteria solution
-W3 bacteria solution
-Ampicillin
-Experimental steps
--10 ml LB medium was added into three glass bottles.
--Add 30ul of acellin into 3 glass bottles respectively.
--Add 2ml of S2 bacteria solution into a glass bottle, and mark S2 l 7.25 on the bottle body.
--Add 2ml of M1 bacteria solution into another glass bottle, and mark M1 l 7.25 on the bottle body.
--Add 2ml of W3 bacteria solution into the last glass bottle, and mark w3l 7.25 on the bottle body.
--Put all glass bottles into a 37 ℃ shaker for cultivation
-/7/25
-Experiment content: enzyme cutting and glue releasing
-Steps:
--Put the rubber plate into the electrophoresis tank and add the electrophoresis buffer.
-- Add 4 μ l marker into the sample hole at one end
--Add 10 μ l enzyme products from left to right in the order of W5, W3, W4, M2, M1, S5, S4, S2 (when the black strip is close to the observer) into the sample hole in turn.
--Add 4 μ l marker to the end sample hole
-- Turn on the electrophoresis instrument, adjust the voltage to 90V, and make the nucleic acid sample swim towards the positive pole (note that the sample hole is at the negative end of the electrophoresis tank). Time: 70 minutes
--After electrophoresis, the power is cut off, the gel is removed, and the electrophoresis results are recorded on the ultraviolet transmittance instrument and recorded.
-Tips:
-. Set alarm clock to remind, check the glue running progress according to the interval, and stop the glue running when the target strip reaches about 1 / 2 of the total glue length.
-S, m and w all run out of two bright bands
-One is about 2000 BP and one is about 1500 bp.
-In line with the ideal situation.
-/7/25
-Experiment content: enzyme cutting
-ul system of S (S2 S4 S5), m (M1 m2), w (W3 W4 W5) plasmids
-x green buffer 2ul
-EcoRI 1ul
-PstI 1ul
-DNA 5ul
-DdH2O 11UL
-step
--Match the above systems
-/7/25
-Experiment content: Plasmid Extraction:
-Raw materials:
-bottles of bacterial solution: S2, S4, S5 M1, M2 W3, W4, W5
-Tube, liquid gun, centrifuge, shaker;
-Kit: Shanghai Huiling Biotechnology Co., Ltd. hlingene high purity plasmid small quantity rapid extraction kit
-Buffer, S2, S3, washer1, eluent.
-Step report:
-Collection and centrifugation: add 1.3ml bacterial solution into 2ml centrifuge tube, centrifugate at 12000rpm for 30s, and discard the supernatant.
-Repeat the above process for three times, and add 3.9ml bacterial solution in total.            Heavy suspension: add 250ul of buffer S1, shake fully until there is no small bacteria block;
-Cracking: add 250ul S2, turn it upside down gently for about 10 times to make the solution clear;
-Neutralization: add 350 UL S3, turn it upside down gently for about 10 times, white floccules appear, centrifugation at 13000 rpm for 10 minutes;
-Collection: take 600ul of supernatant (do not touch the white precipitate), transfer it into the adsorption column of the collection tube, centrifugate it at 12000rpm for 30s, and pour out the waste liquid;
-Washing: add 600ul WB washing solution to the adsorption column, centrifugate at 12000rpm for 30s, and discard the filtrate; repeat once
-Air centrifugation: 13000rpm centrifugation for 2min;
-Replace the column with a new 2ml centrifuge tube.
-Elution centrifugation: add 65ul (50-100ul) eluent, stand for 2min, centrifugation at 12000rpm for 1min.
-Tips:
--A new collecting tube is needed during the process from air centrifugation to elution centrifugation.
--When adding the bacterial solution, it is necessary to see the mark clearly, and the added pipes need to be corresponding.
--Remember to cover the used solution.
--The eluate needs to be preheated in advance.
-According to 260 / 280 (purity) value
-We finally selected S2, M1 and W3 for sequence detection.
-/7/29
-Experiment content: amplification of bacterial solution S4 M2 W5
-raw material
-LB liquid medium
-S4 bacteria solution
-M2 bacteria solution
-W5 bacteria solution
-Ampicillin
-Experimental steps
--4 ml LB medium was added into 3 plastic tubes.
--8 UL acellin was added into 3 plastic tubes respectively.
--Add 20ul of S4 bacterial solution into a plastic tube and mark the bottle with S4 l 7.28.
--Add 20ul of M2 bacteria solution into another plastic tube, and mark M2 l 7.28 on the bottle body.
--Add 20ul of W5 bacteria solution into the last plastic tube, and mark W5 l 7.28 on the bottle body.
--Put all plastic tubes into a 37 ℃ shaker for cultivation
-July 30, 31, 2019
-Experiment content: measuring the growth curve of bacteria
-Raw materials:
-LB medium
-Ampicillin
-S4L bacteria solution
-M2L bacteria solution
-W2L bacteria solution
-Lead acetate solution with multiple dilution
-Steps:
-. Add 5ml LB medium into 6 plastic tubes respectively
-. Add 10ul acellin into these plastic pipes respectively.
-. Add 50ul s4l bacterial solution into these plastic tubes respectively.
-. In the first tube, add 5ul 10 ^ 1 lead acetate solution with the standard of s4l 10 ^ 1 7.30.
-. Add 5ul 10 ^ 2 lead acetate solution in the second tube, and the standard is s4l 10 ^ 2 7.30.
-. In the third tube, add 5ul 10 ^ 3 lead acetate solution with the standard of s4l 10 ^ 3 7.30.
-. Add 5ul 10 ^ 4 lead acetate solution in the fourth tube, and the standard is s4l 10 ^ 4 7.30.
-. Add 5ul 10 ^ 5 lead acetate solution in the fifth tube, marked as s4l 10 ^ 5 7.30.            9. No lead acetate solution is added in the sixth tube, and the standard is s4l 0 7.30.            10. Take 400ul s4l 10 ^ 1 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 1 0h 7.30.
-. Take 400ul s4l 10 ^ 2 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 2 0h 7.30.
-. Take 400ul s4l 10 ^ 3 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 3 0h 7.30.
-. Take 400ul s4l 10 ^ 4 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 4 0h 7.30.
-. Take 400ul s4l 10 ^ 5 7.30 solution and add it into 1.5ml EP tube, which is marked as s 10 ^ 5 0h 7.30.
-. Take 400ul s4l 0 7.30 solution, add it into 1.5ml EP tube, and mark it as s00h 7.30.            16. Put all EP pipes into 4 ℃ for storage.
-. Repeat the above steps for m2l and w5l.
-. After that, 400ul of each plastic pipe shall be sampled every 2 hours and put into 1.5ml EP pipe and marked accordingly (for example, the sample with the date of 7.30 on the same day and swaying for 2h in s4l 10 ^ 1 shall be marked with s 10 ^ 1 2H 7.30) until 8 hours.
+ <p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/16&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Content&nbsp;of&nbsp;the&nbsp;experiment:&nbsp;Colony&nbsp;PCR&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;material:&nbsp;LB&nbsp;medium&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-indent: 96px; text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">chloramphenicol&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-indent: 48px; text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Colony&nbsp;of&nbsp;bacteria&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Take&nbsp;2&nbsp;mL&nbsp;LB&nbsp;culture&nbsp;medium&nbsp;and&nbsp;add&nbsp;it&nbsp;into&nbsp;two&nbsp;2&nbsp;mL&nbsp;EP&nbsp;tubes.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;2&nbsp;uL&nbsp;chloramphenicol&nbsp;was&nbsp;added&nbsp;to&nbsp;the&nbsp;EP&nbsp;tubes.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Blend&nbsp;slightly.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;Take&nbsp;15&nbsp;1.5mL&nbsp;EP&nbsp;tubes.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;200&nbsp;uL&nbsp;medium&nbsp;containing&nbsp;anti-LB&nbsp;was&nbsp;added&nbsp;into&nbsp;EP&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;Five&nbsp;colonies&nbsp;were&nbsp;taken&nbsp;from&nbsp;each&nbsp;Petri&nbsp;dish&nbsp;and&nbsp;added&nbsp;into&nbsp;the&nbsp;corresponding&nbsp;EP&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">7.&nbsp;Shake&nbsp;bacteria&nbsp;on&nbsp;shaking&nbsp;bed&nbsp;at&nbsp;37&nbsp;C&nbsp;for&nbsp;2&nbsp;hours.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Result:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;turbidity&nbsp;of&nbsp;the&nbsp;bacterial&nbsp;liquid&nbsp;indicates&nbsp;that&nbsp;the&nbsp;bacterial&nbsp;body&nbsp;has&nbsp;successfully&nbsp;reproduced&nbsp;and&nbsp;the&nbsp;concentration&nbsp;of&nbsp;the&nbsp;bacterial&nbsp;liquid&nbsp;is&nbsp;high.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;We&nbsp;made&nbsp;three&nbsp;strains,&nbsp;s,&nbsp;W&nbsp;and&nbsp;m.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Chloramphenicol&nbsp;is&nbsp;a&nbsp;screening&nbsp;agent,&nbsp;which&nbsp;can&nbsp;remove&nbsp;impurities.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/18&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Content&nbsp;of&nbsp;the&nbsp;experiment:&nbsp;Measuring&nbsp;OD&nbsp;value&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Eon&nbsp;enzyme&nbsp;labelling&nbsp;instrument&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Diluted&nbsp;S4&nbsp;W1&nbsp;M5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S4&nbsp;W1&nbsp;M5&nbsp;stock&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S3&nbsp;M2&nbsp;W2&nbsp;stock&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;(For&nbsp;detailed&nbsp;steps&nbsp;of&nbsp;multiple&nbsp;dilution,&nbsp;see&nbsp;the&nbsp;relevant&nbsp;documents)&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; 1.&nbsp;Two&nbsp;1.groups&nbsp;of&nbsp;diluted&nbsp;S4&nbsp;W1&nbsp;M5&nbsp;and&nbsp;100ul&nbsp;each&nbsp;were&nbsp;added&nbsp;to&nbsp;the&nbsp;enzyme&nbsp;label&nbsp;plate.&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Put&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;plate&nbsp;into&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;instrument&nbsp;to&nbsp;measure&nbsp;its&nbsp;OD&nbsp;value&nbsp;(see&nbsp;figure&nbsp;for&nbsp;data).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <br style="text-align: left;"/>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;measured&nbsp;OD&nbsp;value&nbsp;is&nbsp;about&nbsp;0.05,&nbsp;not&nbsp;between&nbsp;the&nbsp;most&nbsp;suitable&nbsp;OD&nbsp;value&nbsp;(0.4-0.6),&nbsp;so&nbsp;the&nbsp;raw&nbsp;liquor&nbsp;with&nbsp;higher&nbsp;concentration&nbsp;is&nbsp;taken&nbsp;to&nbsp;measure&nbsp;OD&nbsp;value&nbsp;again.&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <br style="text-align: left;"/>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;The&nbsp;raw&nbsp;liquor&nbsp;of&nbsp;S4&nbsp;W1&nbsp;M5&nbsp;and&nbsp;100ul&nbsp;of&nbsp;each&nbsp;group&nbsp;were&nbsp;added&nbsp;to&nbsp;the&nbsp;enzyme&nbsp;label&nbsp;plate.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;Put&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;plate&nbsp;into&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;instrument&nbsp;to&nbsp;measure&nbsp;its&nbsp;OD&nbsp;value&nbsp;(see&nbsp;figure&nbsp;for&nbsp;data).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;measured&nbsp;OD&nbsp;value&nbsp;is&nbsp;more&nbsp;than&nbsp;0.6,&nbsp;slightly&nbsp;higher&nbsp;than&nbsp;the&nbsp;most&nbsp;suitable&nbsp;OD&nbsp;value&nbsp;(0.4-0.6).&nbsp;Therefore,&nbsp;W&nbsp;1&nbsp;with&nbsp;the&nbsp;smallest&nbsp;difference&nbsp;between&nbsp;the&nbsp;two&nbsp;values&nbsp;(the&nbsp;smallest&nbsp;error)&nbsp;is&nbsp;selected&nbsp;as&nbsp;the&nbsp;ratio&nbsp;dilution.&nbsp;OD&nbsp;value&nbsp;is&nbsp;measured&nbsp;again,&nbsp;and&nbsp;the&nbsp;functional&nbsp;relationship&nbsp;between&nbsp;OD&nbsp;and&nbsp;concentration&nbsp;is&nbsp;established&nbsp;to&nbsp;find&nbsp;the&nbsp;appropriate&nbsp;dilution&nbsp;multiple.&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;Dilute&nbsp;the&nbsp;ratio&nbsp;of&nbsp;raw&nbsp;solution&nbsp;of&nbsp;W1&nbsp;to&nbsp;1,&nbsp;2,&nbsp;4&nbsp;and&nbsp;8&nbsp;times&nbsp;of&nbsp;concentration&nbsp;and&nbsp;add&nbsp;two&nbsp;groups&nbsp;of&nbsp;100ul&nbsp;to&nbsp;the&nbsp;enzyme&nbsp;label&nbsp;plate.&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;Put&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;plate&nbsp;into&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;instrument&nbsp;to&nbsp;measure&nbsp;its&nbsp;OD&nbsp;value&nbsp;(see&nbsp;the&nbsp;yellow&nbsp;part&nbsp;of&nbsp;the&nbsp;icon&nbsp;for&nbsp;the&nbsp;data).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <br style="text-align: left;"/>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;function&nbsp;curve&nbsp;of&nbsp;OD&nbsp;value&nbsp;with&nbsp;respect&nbsp;to&nbsp;concentration&nbsp;is&nbsp;established&nbsp;with&nbsp;Excel&nbsp;table&nbsp;(see&nbsp;figure).&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <br style="text-align: left;"/>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;OD&nbsp;value&nbsp;is&nbsp;0.5926&nbsp;when&nbsp;the&nbsp;OD&nbsp;value&nbsp;is&nbsp;0.4&nbsp;and&nbsp;0.635&nbsp;when&nbsp;the&nbsp;concentration&nbsp;multiple&nbsp;is&nbsp;1,&nbsp;so&nbsp;the&nbsp;dilution&nbsp;multiple&nbsp;should&nbsp;be&nbsp;1/0.5926&nbsp;=&nbsp;1.6874&nbsp;for&nbsp;the&nbsp;original&nbsp;solution&nbsp;of&nbsp;W1.&nbsp;(principle&nbsp;of&nbsp;calculation:&nbsp;concentration&nbsp;=&nbsp;volume&nbsp;/&nbsp;volume,&nbsp;concentration&nbsp;is&nbsp;inversely&nbsp;proportional&nbsp;to&nbsp;volume,&nbsp;and&nbsp;dilution&nbsp;multiple&nbsp;is&nbsp;equal&nbsp;to&nbsp;volume&nbsp;multiplier,&nbsp;so&nbsp;the&nbsp;concentration&nbsp;ratio&nbsp;is&nbsp;inversely&nbsp;proportional&nbsp;to&nbsp;dilution&nbsp;multiple,&nbsp;and&nbsp;the&nbsp;concentration&nbsp;multiple&nbsp;is&nbsp;the&nbsp;reciprocal&nbsp;of&nbsp;dilution&nbsp;multiple).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Because&nbsp;the&nbsp;raw&nbsp;liquor&nbsp;of&nbsp;S4,&nbsp;W1&nbsp;and&nbsp;M5&nbsp;is&nbsp;not&nbsp;much&nbsp;surplus,&nbsp;it&nbsp;is&nbsp;decided&nbsp;to&nbsp;take&nbsp;S3,&nbsp;W2&nbsp;and&nbsp;M2,&nbsp;and&nbsp;to&nbsp;measure&nbsp;the&nbsp;required&nbsp;dilution&nbsp;multiples&nbsp;for&nbsp;use&nbsp;in&nbsp;subsequent&nbsp;inspection.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">7.&nbsp;Two&nbsp;groups&nbsp;of&nbsp;100ul&nbsp;each&nbsp;of&nbsp;S3&nbsp;W2&nbsp;M2&nbsp;bacterial&nbsp;solution&nbsp;were&nbsp;added&nbsp;to&nbsp;the&nbsp;enzyme&nbsp;label&nbsp;plate.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">8.Put&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;plate&nbsp;into&nbsp;the&nbsp;enzyme&nbsp;labeling&nbsp;instrument&nbsp;to&nbsp;measure&nbsp;its&nbsp;OD&nbsp;value&nbsp;(see&nbsp;figure&nbsp;for&nbsp;data).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;measured&nbsp;OD&nbsp;value&nbsp;is&nbsp;about&nbsp;0.7,&nbsp;slightly&nbsp;higher&nbsp;than&nbsp;the&nbsp;most&nbsp;suitable&nbsp;OD&nbsp;value&nbsp;(0.4-0.6),&nbsp;so&nbsp;the&nbsp;average&nbsp;values&nbsp;of&nbsp;S3,&nbsp;W2&nbsp;and&nbsp;M2&nbsp;are&nbsp;taken&nbsp;into&nbsp;the&nbsp;function&nbsp;system.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">9.&nbsp;Calculate&nbsp;the&nbsp;required&nbsp;dilution&nbsp;multiples:</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Result:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">According&nbsp;to&nbsp;the&nbsp;calculation&nbsp;results,&nbsp;it&nbsp;can&nbsp;be&nbsp;seen&nbsp;that&nbsp;the&nbsp;dilution&nbsp;factor&nbsp;is&nbsp;about&nbsp;2&nbsp;times.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/19&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Content&nbsp;of&nbsp;the&nbsp;experiment:&nbsp;Observation&nbsp;of&nbsp;bacteria&nbsp;under&nbsp;ultraviolet&nbsp;radiation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ultraviolet&nbsp;irradiator&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Resuspended&nbsp;centrifuged&nbsp;bacteria&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;groups&nbsp;S,&nbsp;W&nbsp;and&nbsp;M&nbsp;were&nbsp;irradiated&nbsp;by&nbsp;ultraviolet&nbsp;instrument&nbsp;and&nbsp;photographed.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Principle:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">When&nbsp;mCherry&nbsp;is&nbsp;repressed&nbsp;by&nbsp;lead,&nbsp;it&nbsp;expresses&nbsp;fluorescent&nbsp;protein&nbsp;and&nbsp;fluoresces&nbsp;under&nbsp;ultraviolet&nbsp;irradiation.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Result:&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Group&nbsp;S:&nbsp;Compared&nbsp;with&nbsp;the&nbsp;control&nbsp;group,&nbsp;S&nbsp;^&nbsp;10-4&nbsp;showed&nbsp;darker&nbsp;colour,&nbsp;while&nbsp;the&nbsp;other&nbsp;bacteria&nbsp;in&nbsp;lead&nbsp;concentration&nbsp;showed&nbsp;no&nbsp;obvious&nbsp;fluorescence.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Group&nbsp;M:&nbsp;Compared&nbsp;with&nbsp;the&nbsp;control&nbsp;group,&nbsp;M&nbsp;^&nbsp;10-4&nbsp;showed&nbsp;darker&nbsp;colour,&nbsp;while&nbsp;the&nbsp;other&nbsp;bacteria&nbsp;in&nbsp;lead&nbsp;concentration&nbsp;showed&nbsp;no&nbsp;obvious&nbsp;fluorescence.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Group&nbsp;W:&nbsp;Compared&nbsp;with&nbsp;the&nbsp;control&nbsp;group,&nbsp;the&nbsp;bacteria&nbsp;of&nbsp;W&nbsp;^&nbsp;10-4&nbsp;showed&nbsp;a&nbsp;deeper&nbsp;color,&nbsp;and&nbsp;the&nbsp;bacteria&nbsp;with&nbsp;lead&nbsp;concentration&nbsp;of&nbsp;10-7&nbsp;had&nbsp;obvious&nbsp;red&nbsp;fluorescence,&nbsp;while&nbsp;the&nbsp;bacteria&nbsp;with&nbsp;other&nbsp;lead&nbsp;concentration&nbsp;had&nbsp;no&nbsp;obvious&nbsp;fluorescence.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Abnormal&nbsp;situation&nbsp;and&nbsp;analysis:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;W10&nbsp;^&nbsp;-&nbsp;7&nbsp;is&nbsp;the&nbsp;only&nbsp;visible&nbsp;red&nbsp;fluorescence&nbsp;in&nbsp;all&nbsp;bacterial&nbsp;fluids.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;It&nbsp;may&nbsp;be&nbsp;that&nbsp;the&nbsp;high&nbsp;concentration&nbsp;of&nbsp;lead&nbsp;acetate&nbsp;(toxic)&nbsp;leads&nbsp;to&nbsp;the&nbsp;death&nbsp;of&nbsp;other&nbsp;strains,&nbsp;or&nbsp;that&nbsp;mCherry&nbsp;cannot&nbsp;express&nbsp;(emits&nbsp;red&nbsp;fluorescence).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;It&nbsp;may&nbsp;be&nbsp;that&nbsp;in&nbsp;S&nbsp;and&nbsp;M,&nbsp;the&nbsp;concentration&nbsp;of&nbsp;PbrR&nbsp;is&nbsp;too&nbsp;high,&nbsp;or&nbsp;the&nbsp;repression&nbsp;ability&nbsp;is&nbsp;too&nbsp;strong,&nbsp;which&nbsp;results&nbsp;in&nbsp;the&nbsp;absence&nbsp;of&nbsp;mCherry&nbsp;expression&nbsp;in&nbsp;bacterial&nbsp;solution.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;It&nbsp;may&nbsp;be&nbsp;that&nbsp;the&nbsp;system&nbsp;of&nbsp;W10&nbsp;^&nbsp;-&nbsp;7&nbsp;has&nbsp;unexpected&nbsp;abnormalities&nbsp;(mutation&nbsp;of&nbsp;strain&nbsp;(base&nbsp;deletion)&nbsp;or&nbsp;changes&nbsp;in&nbsp;expression.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;S&nbsp;^&nbsp;10-4,&nbsp;M&nbsp;^&nbsp;10-4,&nbsp;W&nbsp;^&nbsp;10-4&nbsp;are&nbsp;darker&nbsp;and&nbsp;some&nbsp;are&nbsp;black.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;It&nbsp;may&nbsp;be&nbsp;that&nbsp;high&nbsp;lead&nbsp;concentration&nbsp;leads&nbsp;to&nbsp;cell&nbsp;death.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/23&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Content&nbsp;of&nbsp;the&nbsp;experiment:&nbsp;Enzyme&nbsp;linkage&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">TaKaRa:&nbsp;DNA&nbsp;Ligation&nbsp;Kit&nbsp;Ver.2.1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Solution&nbsp;I&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Enzymatic&nbsp;digestion&nbsp;products&nbsp;of&nbsp;low-copy&nbsp;skeleton&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Enzymatic&nbsp;Digestion&nbsp;Products&nbsp;of&nbsp;Synthetic&nbsp;Fragments&nbsp;of&nbsp;pPbrR-S4&nbsp;and&nbsp;mCherry&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Enzymatic&nbsp;products&nbsp;of&nbsp;the&nbsp;synthetic&nbsp;fragments&nbsp;of&nbsp;pbrr-m5&nbsp;and&nbsp;mcurry&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Enzymatic&nbsp;Digestion&nbsp;Products&nbsp;of&nbsp;Synthetic&nbsp;Fragments&nbsp;of&nbsp;pPbrR-W1&nbsp;and&nbsp;mCherry&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;DdH2O&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Take&nbsp;4&nbsp;PCR&nbsp;tubes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Solution&nbsp;I&nbsp;with&nbsp;4ul&nbsp;each&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Low-copy&nbsp;skeletons&nbsp;with&nbsp;1&nbsp;UL&nbsp;each&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;The&nbsp;synthetic&nbsp;fragments&nbsp;of&nbsp;pPbrR-S4&nbsp;and&nbsp;mCherry,&nbsp;pPbrR-M5&nbsp;and&nbsp;mCherry,&nbsp;pPbrR-W1&nbsp;and&nbsp;mCherry&nbsp;were&nbsp;added&nbsp;respectively.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;Add&nbsp;1&nbsp;UL&nbsp;of&nbsp;ddH_2O&nbsp;each.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;Set&nbsp;the&nbsp;temperature&nbsp;of&nbsp;16&nbsp;degrees&nbsp;Celsius&nbsp;in&nbsp;the&nbsp;PCR&nbsp;for&nbsp;1&nbsp;hour.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;After&nbsp;the&nbsp;connection,&nbsp;it&nbsp;needs&nbsp;to&nbsp;be&nbsp;instantly&nbsp;transformed&nbsp;into&nbsp;a&nbsp;receptive&nbsp;state.&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><br clear="all" style="break-before: page; text-align: left;"/></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/23&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Content&nbsp;of&nbsp;the&nbsp;experiment:&nbsp;Axygene</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Agarose&nbsp;Gel&nbsp;Containing&nbsp;Purpose&nbsp;DNA&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Buffer&nbsp;DE-A&nbsp;400&nbsp;UL&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Buffer&nbsp;DE-B&nbsp;200&nbsp;ugl&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Buffer&nbsp;W1&nbsp;500&nbsp;ugl&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Buffer&nbsp;W2&nbsp;700&nbsp;mu&nbsp;l*2&nbsp;=&nbsp;1400&nbsp;Mu&nbsp;L&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Eluent&nbsp;25&nbsp;UL&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Instruments:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">blb&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.0&nbsp;ml&nbsp;centrifugal&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.5&nbsp;ml&nbsp;centrifugal&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Water&nbsp;bath&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DNA&nbsp;preparation&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Centrifuge&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Refrigerator&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Cut&nbsp;agarose&nbsp;gel&nbsp;containing&nbsp;target&nbsp;DNA&nbsp;under&nbsp;purple&nbsp;lamp&nbsp;and&nbsp;place&nbsp;it&nbsp;in&nbsp;2.0&nbsp;ml&nbsp;centrifugal&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Add&nbsp;400&nbsp;ml&nbsp;Buffer&nbsp;DE-A,&nbsp;mix&nbsp;evenly,&nbsp;then&nbsp;heat&nbsp;in&nbsp;75&nbsp;C&nbsp;water&nbsp;bath,&nbsp;mix&nbsp;in&nbsp;intervals&nbsp;(every&nbsp;2-3&nbsp;minutes)&nbsp;until&nbsp;gel&nbsp;block&nbsp;melts&nbsp;completely&nbsp;(5&nbsp;minutes).&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Add&nbsp;200&nbsp;ml&nbsp;Buffer&nbsp;DE-B&nbsp;and&nbsp;mix&nbsp;evenly.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;Absorbing&nbsp;the&nbsp;mixture&nbsp;of&nbsp;the&nbsp;above&nbsp;steps&nbsp;and&nbsp;transferring&nbsp;it&nbsp;to&nbsp;the&nbsp;DNA&nbsp;preparation&nbsp;tube&nbsp;(placed&nbsp;in&nbsp;2&nbsp;ml&nbsp;centrifugal&nbsp;tube),&nbsp;centrifuging&nbsp;for&nbsp;1&nbsp;minute&nbsp;at&nbsp;12&nbsp;000*g,&nbsp;discarding&nbsp;the&nbsp;filtrate&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;Put&nbsp;the&nbsp;prepared&nbsp;tube&nbsp;back&nbsp;into&nbsp;centrifugal&nbsp;tube,&nbsp;add&nbsp;500&nbsp;ml&nbsp;buffer&nbsp;W1,&nbsp;12&nbsp;000*g&nbsp;centrifugal&nbsp;for&nbsp;30&nbsp;seconds,&nbsp;discard&nbsp;filtrate&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;he&nbsp;prepared&nbsp;tube&nbsp;was&nbsp;placed&nbsp;back&nbsp;into&nbsp;the&nbsp;centrifugal&nbsp;tube,&nbsp;adding&nbsp;700&nbsp;ml&nbsp;buffer&nbsp;&nbsp;&nbsp;W2,&nbsp;12&nbsp;000*g&nbsp;centrifugal&nbsp;for&nbsp;30&nbsp;seconds,&nbsp;discarding&nbsp;the&nbsp;filtrate.&nbsp;Wash&nbsp;again&nbsp;in&nbsp;the&nbsp;same&nbsp;way&nbsp;with&nbsp;700&nbsp;ml&nbsp;buffer&nbsp;W2&nbsp;and&nbsp;centrifuge&nbsp;for&nbsp;1&nbsp;minute&nbsp;at&nbsp;12000*g.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">7.&nbsp;Place&nbsp;the&nbsp;tube&nbsp;in&nbsp;a&nbsp;2&nbsp;ml&nbsp;centrifugal&nbsp;tube&nbsp;and&nbsp;centrifuge&nbsp;for&nbsp;1&nbsp;min&nbsp;at&nbsp;12000*g.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-&nbsp;The&nbsp;preparation&nbsp;tube&nbsp;was&nbsp;placed&nbsp;in&nbsp;a&nbsp;clean&nbsp;1.5&nbsp;ml&nbsp;centrifugal&nbsp;tube.&nbsp;25&nbsp;ml&nbsp;Eluent&nbsp;was&nbsp;added&nbsp;in&nbsp;the&nbsp;center&nbsp;of&nbsp;the&nbsp;DNA&nbsp;preparation&nbsp;membrane.&nbsp;The&nbsp;tube&nbsp;was&nbsp;placed&nbsp;at&nbsp;room&nbsp;temperature&nbsp;for&nbsp;1&nbsp;minute&nbsp;and&nbsp;centrifuged&nbsp;at&nbsp;12&nbsp;000*g&nbsp;for&nbsp;1&nbsp;minute&nbsp;to&nbsp;elute&nbsp;DNA.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">8.&nbsp;The&nbsp;recovered&nbsp;DNA&nbsp;samples&nbsp;were&nbsp;stored&nbsp;at&nbsp;4&nbsp;C&nbsp;for&nbsp;short-term&nbsp;storage&nbsp;for&nbsp;subsequent&nbsp;experiments.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;Waterbaths&nbsp;should&nbsp;be&nbsp;intermittently&nbsp;checked&nbsp;for&nbsp;gel&nbsp;melting&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;When&nbsp;using&nbsp;centrifuges,&nbsp;attention&nbsp;should&nbsp;be&nbsp;paid&nbsp;to&nbsp;the&nbsp;symmetrical&nbsp;placement&nbsp;of&nbsp;centrifugal&nbsp;tubes.&nbsp;If&nbsp;there&nbsp;are&nbsp;odd&nbsp;number&nbsp;of&nbsp;centrifugal&nbsp;tubes,&nbsp;balance&nbsp;of&nbsp;the&nbsp;total&nbsp;mass&nbsp;of&nbsp;the&nbsp;symmetrical&nbsp;centrifugal&nbsp;tubes&nbsp;(i.e.&nbsp;the&nbsp;sum&nbsp;of&nbsp;the&nbsp;mass&nbsp;of&nbsp;the&nbsp;centrifugal&nbsp;tubes&nbsp;and&nbsp;the&nbsp;mass&nbsp;of&nbsp;the&nbsp;substances&nbsp;therein)&nbsp;should&nbsp;be&nbsp;used.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/23&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Content&nbsp;of&nbsp;the&nbsp;experiment:&nbsp;Purification&nbsp;and&nbsp;validation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Place&nbsp;the&nbsp;plywood&nbsp;in&nbsp;the&nbsp;electrophoresis&nbsp;tank&nbsp;and&nbsp;add&nbsp;the&nbsp;electrophoresis&nbsp;buffer.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Adding&nbsp;4&nbsp;UL&nbsp;marker&nbsp;to&nbsp;an&nbsp;end-point&nbsp;sample&nbsp;hole&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;The&nbsp;purified&nbsp;products&nbsp;were&nbsp;added&nbsp;to&nbsp;1&nbsp;UL&nbsp;Loading&nbsp;Buffer&nbsp;with&nbsp;2&nbsp;UL&nbsp;each.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;The&nbsp;purified&nbsp;products&nbsp;were&nbsp;added&nbsp;into&nbsp;the&nbsp;dot-like&nbsp;pore&nbsp;in&nbsp;the&nbsp;order&nbsp;of&nbsp;vector,&nbsp;S,&nbsp;M&nbsp;and&nbsp;W&nbsp;(when&nbsp;the&nbsp;black&nbsp;band&nbsp;was&nbsp;near&nbsp;the&nbsp;observer)&nbsp;from&nbsp;left&nbsp;to&nbsp;right.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;Adding&nbsp;4&nbsp;ml&nbsp;marker&nbsp;to&nbsp;the&nbsp;end&nbsp;point&nbsp;sample&nbsp;hole</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;Open&nbsp;the&nbsp;electrophoresis&nbsp;apparatus&nbsp;and&nbsp;adjust&nbsp;the&nbsp;voltage&nbsp;to&nbsp;90V&nbsp;to&nbsp;make&nbsp;the&nbsp;nucleic&nbsp;acid&nbsp;sample&nbsp;swim&nbsp;towards&nbsp;the&nbsp;positive&nbsp;pole&nbsp;(note&nbsp;that&nbsp;the&nbsp;sample&nbsp;hole&nbsp;is&nbsp;at&nbsp;the&nbsp;negative&nbsp;end&nbsp;of&nbsp;the&nbsp;electrophoresis&nbsp;tank).&nbsp;Time:&nbsp;40&nbsp;minutes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">After&nbsp;the&nbsp;electrophoresis&nbsp;was&nbsp;completed,&nbsp;the&nbsp;power&nbsp;supply&nbsp;was&nbsp;cut&nbsp;off,&nbsp;the&nbsp;gel&nbsp;was&nbsp;taken&nbsp;out,&nbsp;the&nbsp;electrophoresis&nbsp;results&nbsp;were&nbsp;observed&nbsp;on&nbsp;the&nbsp;ultraviolet&nbsp;transmitter&nbsp;and&nbsp;photographic&nbsp;records&nbsp;were&nbsp;taken.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Set&nbsp;the&nbsp;alarm&nbsp;clock&nbsp;to&nbsp;remind&nbsp;you&nbsp;to&nbsp;check&nbsp;the&nbsp;progress&nbsp;of&nbsp;running&nbsp;glue&nbsp;at&nbsp;intervals&nbsp;and&nbsp;stop&nbsp;running&nbsp;glue&nbsp;when&nbsp;you&nbsp;reach&nbsp;the&nbsp;total&nbsp;progress&nbsp;of&nbsp;2/3&nbsp;from&nbsp;the&nbsp;beginning.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Vector,&nbsp;M,&nbsp;S&nbsp;all&nbsp;run&nbsp;out&nbsp;of&nbsp;the&nbsp;bright&nbsp;band&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">W&nbsp;did&nbsp;not&nbsp;run&nbsp;out&nbsp;of&nbsp;the&nbsp;bright&nbsp;band.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Anomaly&nbsp;analysis:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">It&nbsp;may&nbsp;be&nbsp;that&nbsp;the&nbsp;concentration&nbsp;of&nbsp;W&nbsp;bacterial&nbsp;solution&nbsp;is&nbsp;too&nbsp;low,&nbsp;which&nbsp;leads&nbsp;to&nbsp;the&nbsp;inadequate&nbsp;appearance&nbsp;of&nbsp;bright&nbsp;bands&nbsp;in&nbsp;the&nbsp;proposed&nbsp;plasmid&nbsp;concentration.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/23&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiments:&nbsp;Enzymatic&nbsp;digestion&nbsp;of&nbsp;rubbing&nbsp;gum&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">7.&nbsp;Place&nbsp;the&nbsp;plywood&nbsp;in&nbsp;the&nbsp;electrophoresis&nbsp;tank&nbsp;and&nbsp;add&nbsp;the&nbsp;electrophoresis&nbsp;buffer.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">8.&nbsp;Adding&nbsp;4&nbsp;UL&nbsp;marker&nbsp;to&nbsp;the&nbsp;end-point&nbsp;sample&nbsp;hole&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">9.&nbsp;Put&nbsp;50&nbsp;ml&nbsp;of&nbsp;enzymatic&nbsp;digestion&nbsp;products&nbsp;from&nbsp;left&nbsp;to&nbsp;right&nbsp;in&nbsp;the&nbsp;order&nbsp;of&nbsp;Vector,&nbsp;S,&nbsp;M,&nbsp;W&nbsp;(when&nbsp;the&nbsp;black&nbsp;band&nbsp;is&nbsp;close&nbsp;to&nbsp;the&nbsp;observer)&nbsp;and&nbsp;then&nbsp;add&nbsp;them&nbsp;into&nbsp;the&nbsp;dot-like&nbsp;pore.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">10.&nbsp;Add&nbsp;4&nbsp;ml&nbsp;marker&nbsp;to&nbsp;the&nbsp;end&nbsp;point&nbsp;sample&nbsp;hole&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">11.&nbsp;Open&nbsp;the&nbsp;electrophoresis&nbsp;apparatus&nbsp;and&nbsp;adjust&nbsp;the&nbsp;voltage&nbsp;to&nbsp;90V&nbsp;to&nbsp;make&nbsp;the&nbsp;nucleic&nbsp;acid&nbsp;sample&nbsp;swim&nbsp;towards&nbsp;the&nbsp;positive&nbsp;pole&nbsp;(note&nbsp;that&nbsp;the&nbsp;sample&nbsp;hole&nbsp;is&nbsp;at&nbsp;the&nbsp;negative&nbsp;end&nbsp;of&nbsp;the&nbsp;electrophoresis&nbsp;tank).&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Time:&nbsp;70&nbsp;minutes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;After&nbsp;the&nbsp;electrophoresis&nbsp;was&nbsp;completed,&nbsp;the&nbsp;power&nbsp;supply&nbsp;was&nbsp;cut&nbsp;off,&nbsp;the&nbsp;gel&nbsp;was&nbsp;taken&nbsp;out,&nbsp;the&nbsp;electrophoresis&nbsp;results&nbsp;were&nbsp;observed&nbsp;on&nbsp;the&nbsp;ultraviolet&nbsp;transmitter&nbsp;and&nbsp;photographic&nbsp;records&nbsp;were&nbsp;taken.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Set&nbsp;the&nbsp;alarm&nbsp;clock&nbsp;to&nbsp;remind&nbsp;you&nbsp;to&nbsp;check&nbsp;the&nbsp;progress&nbsp;of&nbsp;running&nbsp;glue&nbsp;at&nbsp;intervals&nbsp;and&nbsp;stop&nbsp;running&nbsp;glue&nbsp;when&nbsp;the&nbsp;target&nbsp;strip&nbsp;reaches&nbsp;about&nbsp;1/2&nbsp;of&nbsp;the&nbsp;total&nbsp;length&nbsp;of&nbsp;the&nbsp;glue.&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S,&nbsp;M&nbsp;and&nbsp;W&nbsp;run&nbsp;out&nbsp;of&nbsp;3000,&nbsp;2000&nbsp;and&nbsp;1500&nbsp;BP&nbsp;bands&nbsp;respectively.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Vector&nbsp;runs&nbsp;out&nbsp;a&nbsp;bright&nbsp;band&nbsp;of&nbsp;about&nbsp;2000&nbsp;bp.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Analysis&nbsp;of&nbsp;abnormal&nbsp;results:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S,&nbsp;M&nbsp;and&nbsp;W&nbsp;run&nbsp;out&nbsp;of&nbsp;the&nbsp;bright&nbsp;band&nbsp;of&nbsp;about&nbsp;3000bp&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;It&nbsp;may&nbsp;be&nbsp;that&nbsp;the&nbsp;short&nbsp;cleavage&nbsp;time&nbsp;resulted&nbsp;in&nbsp;the&nbsp;failure&nbsp;of&nbsp;some&nbsp;S,&nbsp;M&nbsp;and&nbsp;W&nbsp;plasmids&nbsp;to&nbsp;be&nbsp;cut&nbsp;off.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/23&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Plasmid&nbsp;extraction:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1&nbsp;Bottle&nbsp;of&nbsp;Bacterial&nbsp;Solution:&nbsp;W1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Test&nbsp;tube,&nbsp;liquid&nbsp;gun,&nbsp;centrifuge,&nbsp;oscillator;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Kit:&nbsp;Hlingene&nbsp;High&nbsp;Purity&nbsp;Plasmid&nbsp;Small&nbsp;Quantity&nbsp;Rapid&nbsp;Extraction&nbsp;Kit,&nbsp;Shanghai&nbsp;Huiling&nbsp;Biotechnology&nbsp;Co.,&nbsp;Ltd.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Buffer&nbsp;(Protecting&nbsp;DNA,&nbsp;Resuspending&nbsp;DNA),&nbsp;S2&nbsp;(Alkaline&nbsp;lysis),&nbsp;S3&nbsp;(Acidic&nbsp;Neutralizer&nbsp;+&nbsp;Protein&nbsp;Precipitation&nbsp;Promotion),&nbsp;washer&nbsp;1,&nbsp;Eluent.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Step&nbsp;report:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Collection&nbsp;centrifugation:&nbsp;1.5&nbsp;ml&nbsp;centrifugal&nbsp;tube&nbsp;with&nbsp;1.3&nbsp;ml&nbsp;bacterial&nbsp;solution,&nbsp;12&nbsp;000&nbsp;rpm&nbsp;centrifugation&nbsp;for&nbsp;30&nbsp;seconds,&nbsp;discarding&nbsp;supernatant&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Repeat&nbsp;the&nbsp;above&nbsp;process&nbsp;4&nbsp;times&nbsp;and&nbsp;add&nbsp;5.2&nbsp;ml&nbsp;bacterial&nbsp;solution.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Resuspension:&nbsp;adding&nbsp;500&nbsp;UL&nbsp;buffer&nbsp;S1,&nbsp;the&nbsp;shock&nbsp;is&nbsp;sufficient&nbsp;to&nbsp;completely&nbsp;free&nbsp;of&nbsp;small&nbsp;bacteria;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Move&nbsp;all&nbsp;solutions&nbsp;to&nbsp;2ml&nbsp;EP&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Pyrolysis:&nbsp;Add&nbsp;500&nbsp;UL&nbsp;of&nbsp;S&nbsp;2,&nbsp;gently&nbsp;inverted&nbsp;about&nbsp;10&nbsp;times,&nbsp;so&nbsp;that&nbsp;the&nbsp;solution&nbsp;becomes&nbsp;clear;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Neutralization:&nbsp;adding&nbsp;700ul&nbsp;S3,&nbsp;gently&nbsp;inverted&nbsp;about&nbsp;10&nbsp;times,&nbsp;white&nbsp;flocculent&nbsp;objects&nbsp;appeared,&nbsp;centrifuged&nbsp;at&nbsp;13000&nbsp;rpm&nbsp;for&nbsp;15&nbsp;minutes;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Collection:&nbsp;absorb&nbsp;supernatant&nbsp;(do&nbsp;not&nbsp;touch&nbsp;white&nbsp;precipitation)&nbsp;600ul,&nbsp;transfer&nbsp;it&nbsp;into&nbsp;the&nbsp;adsorption&nbsp;column&nbsp;of&nbsp;the&nbsp;collection&nbsp;tube,&nbsp;centrifuge&nbsp;at&nbsp;12000&nbsp;rpm&nbsp;for&nbsp;30&nbsp;seconds,&nbsp;and&nbsp;pour&nbsp;out&nbsp;the&nbsp;waste&nbsp;liquid;&nbsp;repeat&nbsp;the&nbsp;above&nbsp;action&nbsp;once&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Cleaning:&nbsp;add&nbsp;600ul&nbsp;WB&nbsp;lotion&nbsp;to&nbsp;the&nbsp;adsorption&nbsp;column,&nbsp;centrifuge&nbsp;at&nbsp;12000rpm&nbsp;for&nbsp;30s,&nbsp;discard&nbsp;the&nbsp;filtrate,&nbsp;and&nbsp;repeat&nbsp;it.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Air&nbsp;centrifugation:&nbsp;12000&nbsp;RPM&nbsp;centrifugation&nbsp;for&nbsp;30&nbsp;seconds;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;column&nbsp;was&nbsp;replaced&nbsp;by&nbsp;a&nbsp;new&nbsp;2ml&nbsp;centrifugal&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Elution&nbsp;of&nbsp;the&nbsp;heart:&nbsp;add&nbsp;65ul&nbsp;(50-100ul)&nbsp;eluent,&nbsp;stand&nbsp;for&nbsp;2&nbsp;minutes,&nbsp;centrifuge&nbsp;for&nbsp;1&nbsp;minute&nbsp;at&nbsp;12000&nbsp;rpm.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;New&nbsp;collecting&nbsp;tubes&nbsp;need&nbsp;to&nbsp;be&nbsp;replaced&nbsp;from&nbsp;centrifugation&nbsp;to&nbsp;heart&nbsp;washing.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.&nbsp;When&nbsp;adding&nbsp;bacterial&nbsp;liquid,&nbsp;it&nbsp;is&nbsp;necessary&nbsp;to&nbsp;see&nbsp;the&nbsp;mark&nbsp;clearly,&nbsp;and&nbsp;the&nbsp;pipes&nbsp;need&nbsp;to&nbsp;be&nbsp;corresponded.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Use&nbsp;up&nbsp;the&nbsp;solution&nbsp;and&nbsp;remember&nbsp;to&nbsp;cover&nbsp;it.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;The&nbsp;eluent&nbsp;needs&nbsp;to&nbsp;be&nbsp;preheated&nbsp;in&nbsp;advance.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Concentration&nbsp;132.5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Abnormal:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;The&nbsp;centrifuged&nbsp;S&nbsp;and&nbsp;M&nbsp;are&nbsp;reddish,&nbsp;the&nbsp;reason&nbsp;is&nbsp;unknown.&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/24&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;enzyme&nbsp;cutting&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Pbrr-s&nbsp;50ul&nbsp;system：&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">10x&nbsp;green&nbsp;buffer&nbsp;5ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EcoRI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">PstI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ppbrr-s&nbsp;14ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DdH2O&nbsp;27ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Pbrr-m&nbsp;50ul&nbsp;system：&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">10x&nbsp;green&nbsp;buffer&nbsp;5ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EcoRI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">PstI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ppbrr-m&nbsp;22ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DdH2O&nbsp;19ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Pbrr-w&nbsp;50ul&nbsp;system：&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">10x&nbsp;green&nbsp;buffer&nbsp;5ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EcoRI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">PstI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ppbrr-w&nbsp;41ul&nbsp;(41.9ul&nbsp;should&nbsp;have&nbsp;been&nbsp;added)&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DdH2O&nbsp;0ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Low&nbsp;copy&nbsp;skeleton&nbsp;vector&nbsp;50ul&nbsp;system：&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">10x&nbsp;green&nbsp;buffer&nbsp;5ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EcoRI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">PstI&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Skeleton&nbsp;plasmid&nbsp;14ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DdH2O&nbsp;27ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">step&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Match&nbsp;the&nbsp;above&nbsp;systems&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Put&nbsp;in&nbsp;the&nbsp;PCR&nbsp;instrument,&nbsp;37&nbsp;℃,&nbsp;45&nbsp;minutes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Enzyme&nbsp;should&nbsp;be&nbsp;added&nbsp;first&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/24&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;PCR&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">20ul&nbsp;system&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Taq&nbsp;mix&nbsp;(2x)&nbsp;10ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Primer&nbsp;(F1)&nbsp;1ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Primer&nbsp;(R2)&nbsp;1ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;Template&nbsp;(bacterial&nbsp;solution)&nbsp;1ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;DdH2O&nbsp;7ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">PCR&nbsp;process:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Set&nbsp;20ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Start:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">94&nbsp;℃&nbsp;5min&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Cycle&nbsp;step&nbsp;(34&nbsp;times):&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">94&nbsp;℃&nbsp;30s&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">55&nbsp;℃&nbsp;30s&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">72&nbsp;℃&nbsp;1min&nbsp;40s&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">End:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">72&nbsp;℃&nbsp;5min&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Finally&nbsp;placed&nbsp;at&nbsp;4&nbsp;℃&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Taq&nbsp;2x&nbsp;Mix&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Up&nbsp;primer&nbsp;F1&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Lower&nbsp;primer&nbsp;R2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">bacterial&nbsp;fluid&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DdH2O&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Mark&nbsp;16&nbsp;PCR&nbsp;tubes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Add&nbsp;10ul&nbsp;Taq&nbsp;enzyme&nbsp;solution&nbsp;to&nbsp;each&nbsp;PCR&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Add&nbsp;7ul&nbsp;ddH2O&nbsp;into&nbsp;each&nbsp;PCR&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;Add&nbsp;1ul&nbsp;F1&nbsp;primer&nbsp;to&nbsp;each&nbsp;PCR&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;Add&nbsp;1ul&nbsp;R2&nbsp;primer&nbsp;to&nbsp;each&nbsp;PCR&nbsp;tube&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;Add&nbsp;1ul&nbsp;s&nbsp;bacteria&nbsp;solution&nbsp;to&nbsp;5&nbsp;tubes,&nbsp;1ul&nbsp;m&nbsp;bacteria&nbsp;solution&nbsp;to&nbsp;5&nbsp;tubes,&nbsp;1ul&nbsp;w&nbsp;bacteria&nbsp;solution&nbsp;to&nbsp;5&nbsp;tubes,&nbsp;and&nbsp;1ul&nbsp;ddH2O&nbsp;to&nbsp;the&nbsp;remaining&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">7.&nbsp;Put&nbsp;all&nbsp;configuration&nbsp;products&nbsp;into&nbsp;PCR&nbsp;machine&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">8.&nbsp;Set&nbsp;up&nbsp;PCR&nbsp;instrument:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Set&nbsp;volume&nbsp;to&nbsp;20ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">The&nbsp;pre&nbsp;denaturation&nbsp;temperature&nbsp;is&nbsp;94&nbsp;℃,&nbsp;5&nbsp;minutes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Repeat&nbsp;the&nbsp;following&nbsp;three&nbsp;steps&nbsp;34&nbsp;times&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Denaturation&nbsp;temperature&nbsp;94&nbsp;℃,&nbsp;30&nbsp;seconds&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Annealing&nbsp;temperature&nbsp;55&nbsp;℃,&nbsp;30&nbsp;seconds&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Extension&nbsp;temperature&nbsp;72&nbsp;℃,&nbsp;1&nbsp;minute&nbsp;40&nbsp;seconds&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Set&nbsp;to&nbsp;stay&nbsp;at&nbsp;72&nbsp;℃&nbsp;for&nbsp;5&nbsp;minutes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Last&nbsp;stop&nbsp;at&nbsp;4&nbsp;℃&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;When&nbsp;matching&nbsp;the&nbsp;PCR&nbsp;system,&nbsp;it&nbsp;is&nbsp;necessary&nbsp;to&nbsp;confirm&nbsp;whether&nbsp;the&nbsp;measurement&nbsp;and&nbsp;proportion&nbsp;are&nbsp;accurate.&nbsp;If&nbsp;bubbles&nbsp;appear,&nbsp;it&nbsp;is&nbsp;necessary&nbsp;to&nbsp;take&nbsp;them&nbsp;again.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;When&nbsp;adding&nbsp;bacterial&nbsp;solution&nbsp;to&nbsp;PCR&nbsp;tube,&nbsp;it&nbsp;is&nbsp;necessary&nbsp;to&nbsp;confirm&nbsp;whether&nbsp;it&nbsp;corresponds&nbsp;to&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Pay&nbsp;attention&nbsp;to&nbsp;the&nbsp;accuracy&nbsp;of&nbsp;input&nbsp;value&nbsp;when&nbsp;setting&nbsp;up&nbsp;PCR&nbsp;instrument&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;S1&nbsp;did&nbsp;not&nbsp;run&nbsp;out&nbsp;of&nbsp;bright&nbsp;band,&nbsp;S2,&nbsp;S3,&nbsp;S4,&nbsp;S5&nbsp;all&nbsp;ran&nbsp;out&nbsp;of&nbsp;bright&nbsp;band.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;M3&nbsp;and&nbsp;M4&nbsp;did&nbsp;not&nbsp;run&nbsp;out&nbsp;of&nbsp;bright&nbsp;band,&nbsp;M1,&nbsp;M2&nbsp;and&nbsp;M5&nbsp;all&nbsp;ran&nbsp;out&nbsp;of&nbsp;bright&nbsp;band.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;W&nbsp;ran&nbsp;out&nbsp;of&nbsp;the&nbsp;bright&nbsp;band.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">(Abnormal)&nbsp;There&nbsp;is&nbsp;a&nbsp;dark&nbsp;band&nbsp;in&nbsp;the&nbsp;negative&nbsp;control,&nbsp;the&nbsp;reason&nbsp;is&nbsp;unknown.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/24&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;EC20&nbsp;2&nbsp;SMTA&nbsp;2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">raw&nbsp;material&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">LB&nbsp;liquid&nbsp;medium&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EC20&nbsp;2&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">SmtA&nbsp;2&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Experimental&nbsp;steps&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EC20&nbsp;2&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-20&nbsp;ml&nbsp;LB&nbsp;medium&nbsp;was&nbsp;added&nbsp;into&nbsp;a&nbsp;large&nbsp;glass&nbsp;bottle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;40&nbsp;μ&nbsp;L&nbsp;acellin&nbsp;to&nbsp;the&nbsp;glass&nbsp;bottle,&nbsp;and&nbsp;mark&nbsp;ec202&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;80&nbsp;μ&nbsp;L&nbsp;ec202&nbsp;bacterial&nbsp;solution&nbsp;into&nbsp;the&nbsp;glass&nbsp;bottle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">SmtA&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-5&nbsp;ml&nbsp;of&nbsp;LB&nbsp;medium&nbsp;was&nbsp;added&nbsp;to&nbsp;the&nbsp;other&nbsp;four&nbsp;glass&nbsp;bottles,&nbsp;and&nbsp;SMTA&nbsp;2&nbsp;was&nbsp;labeled&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;10&nbsp;μ&nbsp;l&nbsp;of&nbsp;acellin&nbsp;into&nbsp;the&nbsp;glass&nbsp;bottle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;20&nbsp;UL&nbsp;of&nbsp;SMTA&nbsp;2&nbsp;solution&nbsp;to&nbsp;the&nbsp;glass&nbsp;bottle.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Put&nbsp;all&nbsp;glass&nbsp;bottles&nbsp;into&nbsp;a&nbsp;37&nbsp;℃&nbsp;shaker&nbsp;for&nbsp;cultivation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/24&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;amplification&nbsp;of&nbsp;SL&nbsp;ml&nbsp;WL&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">raw&nbsp;material&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">LB&nbsp;liquid&nbsp;medium&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">SL&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">ML&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">WL&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Experimental&nbsp;steps&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-4&nbsp;ml&nbsp;LB&nbsp;medium&nbsp;was&nbsp;added&nbsp;into&nbsp;15&nbsp;glass&nbsp;bottles.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;8ul&nbsp;acellin&nbsp;to&nbsp;15&nbsp;glass&nbsp;bottles.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;10&nbsp;μ&nbsp;l&nbsp;of&nbsp;bacteria&nbsp;solution&nbsp;containing&nbsp;s&nbsp;into&nbsp;five&nbsp;glass&nbsp;bottles&nbsp;and&nbsp;mark&nbsp;SL&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;10&nbsp;μ&nbsp;l&nbsp;of&nbsp;bacteria&nbsp;solution&nbsp;containing&nbsp;m&nbsp;into&nbsp;the&nbsp;other&nbsp;five&nbsp;glass&nbsp;bottles&nbsp;and&nbsp;mark&nbsp;ml&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;10&nbsp;μ&nbsp;l&nbsp;of&nbsp;W&nbsp;bacteria&nbsp;solution&nbsp;into&nbsp;the&nbsp;last&nbsp;five&nbsp;glass&nbsp;bottles,&nbsp;and&nbsp;mark&nbsp;WL&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Put&nbsp;all&nbsp;glass&nbsp;bottles&nbsp;into&nbsp;a&nbsp;37&nbsp;℃&nbsp;shaker&nbsp;for&nbsp;cultivation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/25&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;amplified&nbsp;bacterial&nbsp;solution&nbsp;S2&nbsp;M1&nbsp;W3&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">raw&nbsp;material&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">LB&nbsp;liquid&nbsp;medium&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S2&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">M1&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">W3&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ampicillin&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Experimental&nbsp;steps&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-10&nbsp;ml&nbsp;LB&nbsp;medium&nbsp;was&nbsp;added&nbsp;into&nbsp;three&nbsp;glass&nbsp;bottles.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;30ul&nbsp;of&nbsp;acellin&nbsp;into&nbsp;3&nbsp;glass&nbsp;bottles&nbsp;respectively.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;2ml&nbsp;of&nbsp;S2&nbsp;bacteria&nbsp;solution&nbsp;into&nbsp;a&nbsp;glass&nbsp;bottle,&nbsp;and&nbsp;mark&nbsp;S2&nbsp;l&nbsp;7.25&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;2ml&nbsp;of&nbsp;M1&nbsp;bacteria&nbsp;solution&nbsp;into&nbsp;another&nbsp;glass&nbsp;bottle,&nbsp;and&nbsp;mark&nbsp;M1&nbsp;l&nbsp;7.25&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;2ml&nbsp;of&nbsp;W3&nbsp;bacteria&nbsp;solution&nbsp;into&nbsp;the&nbsp;last&nbsp;glass&nbsp;bottle,&nbsp;and&nbsp;mark&nbsp;w3l&nbsp;7.25&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Put&nbsp;all&nbsp;glass&nbsp;bottles&nbsp;into&nbsp;a&nbsp;37&nbsp;℃&nbsp;shaker&nbsp;for&nbsp;cultivation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/25&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;enzyme&nbsp;cutting&nbsp;and&nbsp;glue&nbsp;releasing&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Put&nbsp;the&nbsp;rubber&nbsp;plate&nbsp;into&nbsp;the&nbsp;electrophoresis&nbsp;tank&nbsp;and&nbsp;add&nbsp;the&nbsp;electrophoresis&nbsp;buffer.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;Add&nbsp;4&nbsp;μ&nbsp;l&nbsp;marker&nbsp;into&nbsp;the&nbsp;sample&nbsp;hole&nbsp;at&nbsp;one&nbsp;end&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;10&nbsp;μ&nbsp;l&nbsp;enzyme&nbsp;products&nbsp;from&nbsp;left&nbsp;to&nbsp;right&nbsp;in&nbsp;the&nbsp;order&nbsp;of&nbsp;W5,&nbsp;W3,&nbsp;W4,&nbsp;M2,&nbsp;M1,&nbsp;S5,&nbsp;S4,&nbsp;S2&nbsp;(when&nbsp;the&nbsp;black&nbsp;strip&nbsp;is&nbsp;close&nbsp;to&nbsp;the&nbsp;observer)&nbsp;into&nbsp;the&nbsp;sample&nbsp;hole&nbsp;in&nbsp;turn.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;4&nbsp;μ&nbsp;l&nbsp;marker&nbsp;to&nbsp;the&nbsp;end&nbsp;sample&nbsp;hole&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-&nbsp;Turn&nbsp;on&nbsp;the&nbsp;electrophoresis&nbsp;instrument,&nbsp;adjust&nbsp;the&nbsp;voltage&nbsp;to&nbsp;90V,&nbsp;and&nbsp;make&nbsp;the&nbsp;nucleic&nbsp;acid&nbsp;sample&nbsp;swim&nbsp;towards&nbsp;the&nbsp;positive&nbsp;pole&nbsp;(note&nbsp;that&nbsp;the&nbsp;sample&nbsp;hole&nbsp;is&nbsp;at&nbsp;the&nbsp;negative&nbsp;end&nbsp;of&nbsp;the&nbsp;electrophoresis&nbsp;tank).&nbsp;Time:&nbsp;70&nbsp;minutes&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-After&nbsp;electrophoresis,&nbsp;the&nbsp;power&nbsp;is&nbsp;cut&nbsp;off,&nbsp;the&nbsp;gel&nbsp;is&nbsp;removed,&nbsp;and&nbsp;the&nbsp;electrophoresis&nbsp;results&nbsp;are&nbsp;recorded&nbsp;on&nbsp;the&nbsp;ultraviolet&nbsp;transmittance&nbsp;instrument&nbsp;and&nbsp;recorded.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Set&nbsp;alarm&nbsp;clock&nbsp;to&nbsp;remind,&nbsp;check&nbsp;the&nbsp;glue&nbsp;running&nbsp;progress&nbsp;according&nbsp;to&nbsp;the&nbsp;interval,&nbsp;and&nbsp;stop&nbsp;the&nbsp;glue&nbsp;running&nbsp;when&nbsp;the&nbsp;target&nbsp;strip&nbsp;reaches&nbsp;about&nbsp;1&nbsp;/&nbsp;2&nbsp;of&nbsp;the&nbsp;total&nbsp;glue&nbsp;length.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S,&nbsp;m&nbsp;and&nbsp;w&nbsp;all&nbsp;run&nbsp;out&nbsp;of&nbsp;two&nbsp;bright&nbsp;bands&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">One&nbsp;is&nbsp;about&nbsp;2000&nbsp;BP&nbsp;and&nbsp;one&nbsp;is&nbsp;about&nbsp;1500&nbsp;bp.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">In&nbsp;line&nbsp;with&nbsp;the&nbsp;ideal&nbsp;situation.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/25&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;enzyme&nbsp;cutting&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">20ul&nbsp;system&nbsp;of&nbsp;S&nbsp;(S2&nbsp;S4&nbsp;S5),&nbsp;m&nbsp;(M1&nbsp;m2),&nbsp;w&nbsp;(W3&nbsp;W4&nbsp;W5)&nbsp;plasmids&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">10x&nbsp;green&nbsp;buffer&nbsp;2ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">EcoRI&nbsp;1ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">PstI&nbsp;1ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DNA&nbsp;5ul&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">DdH2O&nbsp;11UL&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">step&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Match&nbsp;the&nbsp;above&nbsp;systems</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/25&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;Plasmid&nbsp;Extraction:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">8&nbsp;bottles&nbsp;of&nbsp;bacterial&nbsp;solution:&nbsp;S2,&nbsp;S4,&nbsp;S5&nbsp;M1,&nbsp;M2&nbsp;W3,&nbsp;W4,&nbsp;W5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tube,&nbsp;liquid&nbsp;gun,&nbsp;centrifuge,&nbsp;shaker;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Kit:&nbsp;Shanghai&nbsp;Huiling&nbsp;Biotechnology&nbsp;Co.,&nbsp;Ltd.&nbsp;hlingene&nbsp;high&nbsp;purity&nbsp;plasmid&nbsp;small&nbsp;quantity&nbsp;rapid&nbsp;extraction&nbsp;kit&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Buffer,&nbsp;S2,&nbsp;S3,&nbsp;washer1,&nbsp;eluent.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Step&nbsp;report:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Collection&nbsp;and&nbsp;centrifugation:&nbsp;add&nbsp;1.3ml&nbsp;bacterial&nbsp;solution&nbsp;into&nbsp;2ml&nbsp;centrifuge&nbsp;tube,&nbsp;centrifugate&nbsp;at&nbsp;12000rpm&nbsp;for&nbsp;30s,&nbsp;and&nbsp;discard&nbsp;the&nbsp;supernatant.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Repeat&nbsp;the&nbsp;above&nbsp;process&nbsp;for&nbsp;three&nbsp;times,&nbsp;and&nbsp;add&nbsp;3.9ml&nbsp;bacterial&nbsp;solution&nbsp;in&nbsp;total.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Heavy&nbsp;suspension:&nbsp;add&nbsp;250ul&nbsp;of&nbsp;buffer&nbsp;S1,&nbsp;shake&nbsp;fully&nbsp;until&nbsp;there&nbsp;is&nbsp;no&nbsp;small&nbsp;bacteria&nbsp;block;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Cracking:&nbsp;add&nbsp;250ul&nbsp;S2,&nbsp;turn&nbsp;it&nbsp;upside&nbsp;down&nbsp;gently&nbsp;for&nbsp;about&nbsp;10&nbsp;times&nbsp;to&nbsp;make&nbsp;the&nbsp;solution&nbsp;clear;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Neutralization:&nbsp;add&nbsp;350&nbsp;UL&nbsp;S3,&nbsp;turn&nbsp;it&nbsp;upside&nbsp;down&nbsp;gently&nbsp;for&nbsp;about&nbsp;10&nbsp;times,&nbsp;white&nbsp;floccules&nbsp;appear,&nbsp;centrifugation&nbsp;at&nbsp;13000&nbsp;rpm&nbsp;for&nbsp;10&nbsp;minutes;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Collection:&nbsp;take&nbsp;600ul&nbsp;of&nbsp;supernatant&nbsp;(do&nbsp;not&nbsp;touch&nbsp;the&nbsp;white&nbsp;precipitate),&nbsp;transfer&nbsp;it&nbsp;into&nbsp;the&nbsp;adsorption&nbsp;column&nbsp;of&nbsp;the&nbsp;collection&nbsp;tube,&nbsp;centrifugate&nbsp;it&nbsp;at&nbsp;12000rpm&nbsp;for&nbsp;30s,&nbsp;and&nbsp;pour&nbsp;out&nbsp;the&nbsp;waste&nbsp;liquid;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Washing:&nbsp;add&nbsp;600ul&nbsp;WB&nbsp;washing&nbsp;solution&nbsp;to&nbsp;the&nbsp;adsorption&nbsp;column,&nbsp;centrifugate&nbsp;at&nbsp;12000rpm&nbsp;for&nbsp;30s,&nbsp;and&nbsp;discard&nbsp;the&nbsp;filtrate;&nbsp;repeat&nbsp;once&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Air&nbsp;centrifugation:&nbsp;13000rpm&nbsp;centrifugation&nbsp;for&nbsp;2min;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Replace&nbsp;the&nbsp;column&nbsp;with&nbsp;a&nbsp;new&nbsp;2ml&nbsp;centrifuge&nbsp;tube.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Elution&nbsp;centrifugation:&nbsp;add&nbsp;65ul&nbsp;(50-100ul)&nbsp;eluent,&nbsp;stand&nbsp;for&nbsp;2min,&nbsp;centrifugation&nbsp;at&nbsp;12000rpm&nbsp;for&nbsp;1min.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Tips:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-A&nbsp;new&nbsp;collecting&nbsp;tube&nbsp;is&nbsp;needed&nbsp;during&nbsp;the&nbsp;process&nbsp;from&nbsp;air&nbsp;centrifugation&nbsp;to&nbsp;elution&nbsp;centrifugation.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-When&nbsp;adding&nbsp;the&nbsp;bacterial&nbsp;solution,&nbsp;it&nbsp;is&nbsp;necessary&nbsp;to&nbsp;see&nbsp;the&nbsp;mark&nbsp;clearly,&nbsp;and&nbsp;the&nbsp;added&nbsp;pipes&nbsp;need&nbsp;to&nbsp;be&nbsp;corresponding.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Remember&nbsp;to&nbsp;cover&nbsp;the&nbsp;used&nbsp;solution.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-The&nbsp;eluate&nbsp;needs&nbsp;to&nbsp;be&nbsp;preheated&nbsp;in&nbsp;advance.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">According&nbsp;to&nbsp;260&nbsp;/&nbsp;280&nbsp;(purity)&nbsp;value&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">We&nbsp;finally&nbsp;selected&nbsp;S2,&nbsp;M1&nbsp;and&nbsp;W3&nbsp;for&nbsp;sequence&nbsp;detection.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">2019/7/29&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;amplification&nbsp;of&nbsp;bacterial&nbsp;solution&nbsp;S4&nbsp;M2&nbsp;W5&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">raw&nbsp;material&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">LB&nbsp;liquid&nbsp;medium&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S4&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">M2&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">W5&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ampicillin&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Experimental&nbsp;steps&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-4&nbsp;ml&nbsp;LB&nbsp;medium&nbsp;was&nbsp;added&nbsp;into&nbsp;3&nbsp;plastic&nbsp;tubes.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-8&nbsp;UL&nbsp;acellin&nbsp;was&nbsp;added&nbsp;into&nbsp;3&nbsp;plastic&nbsp;tubes&nbsp;respectively.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;20ul&nbsp;of&nbsp;S4&nbsp;bacterial&nbsp;solution&nbsp;into&nbsp;a&nbsp;plastic&nbsp;tube&nbsp;and&nbsp;mark&nbsp;the&nbsp;bottle&nbsp;with&nbsp;S4&nbsp;l&nbsp;7.28.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;20ul&nbsp;of&nbsp;M2&nbsp;bacteria&nbsp;solution&nbsp;into&nbsp;another&nbsp;plastic&nbsp;tube,&nbsp;and&nbsp;mark&nbsp;M2&nbsp;l&nbsp;7.28&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Add&nbsp;20ul&nbsp;of&nbsp;W5&nbsp;bacteria&nbsp;solution&nbsp;into&nbsp;the&nbsp;last&nbsp;plastic&nbsp;tube,&nbsp;and&nbsp;mark&nbsp;W5&nbsp;l&nbsp;7.28&nbsp;on&nbsp;the&nbsp;bottle&nbsp;body.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">-Put&nbsp;all&nbsp;plastic&nbsp;tubes&nbsp;into&nbsp;a&nbsp;37&nbsp;℃&nbsp;shaker&nbsp;for&nbsp;cultivation&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">July&nbsp;30,&nbsp;31,&nbsp;2019&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;"><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;">Experiment&nbsp;content:&nbsp;measuring&nbsp;the&nbsp;growth&nbsp;curve&nbsp;of&nbsp;bacteria&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></strong><strong><span style="font-family: 微软雅黑, &quot;Microsoft YaHei&quot;; color: rgb(0, 0, 0); font-size: 16px;"></span></strong></span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Raw&nbsp;materials:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">LB&nbsp;medium&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Ampicillin&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">S4L&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">M2L&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">W2L&nbsp;bacteria&nbsp;solution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Lead&nbsp;acetate&nbsp;solution&nbsp;with&nbsp;multiple&nbsp;dilution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">Steps:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">1.&nbsp;Add&nbsp;5ml&nbsp;LB&nbsp;medium&nbsp;into&nbsp;6&nbsp;plastic&nbsp;tubes&nbsp;respectively&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">2.&nbsp;Add&nbsp;10ul&nbsp;acellin&nbsp;into&nbsp;these&nbsp;plastic&nbsp;pipes&nbsp;respectively.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">3.&nbsp;Add&nbsp;50ul&nbsp;s4l&nbsp;bacterial&nbsp;solution&nbsp;into&nbsp;these&nbsp;plastic&nbsp;tubes&nbsp;respectively.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">4.&nbsp;In&nbsp;the&nbsp;first&nbsp;tube,&nbsp;add&nbsp;5ul&nbsp;10&nbsp;^&nbsp;1&nbsp;lead&nbsp;acetate&nbsp;solution&nbsp;with&nbsp;the&nbsp;standard&nbsp;of&nbsp;s4l&nbsp;10&nbsp;^&nbsp;1&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">5.&nbsp;Add&nbsp;5ul&nbsp;10&nbsp;^&nbsp;2&nbsp;lead&nbsp;acetate&nbsp;solution&nbsp;in&nbsp;the&nbsp;second&nbsp;tube,&nbsp;and&nbsp;the&nbsp;standard&nbsp;is&nbsp;s4l&nbsp;10&nbsp;^&nbsp;2&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">6.&nbsp;In&nbsp;the&nbsp;third&nbsp;tube,&nbsp;add&nbsp;5ul&nbsp;10&nbsp;^&nbsp;3&nbsp;lead&nbsp;acetate&nbsp;solution&nbsp;with&nbsp;the&nbsp;standard&nbsp;of&nbsp;s4l&nbsp;10&nbsp;^&nbsp;3&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">7.&nbsp;Add&nbsp;5ul&nbsp;10&nbsp;^&nbsp;4&nbsp;lead&nbsp;acetate&nbsp;solution&nbsp;in&nbsp;the&nbsp;fourth&nbsp;tube,&nbsp;and&nbsp;the&nbsp;standard&nbsp;is&nbsp;s4l&nbsp;10&nbsp;^&nbsp;4&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">8.&nbsp;Add&nbsp;5ul&nbsp;10&nbsp;^&nbsp;5&nbsp;lead&nbsp;acetate&nbsp;solution&nbsp;in&nbsp;the&nbsp;fifth&nbsp;tube,&nbsp;marked&nbsp;as&nbsp;s4l&nbsp;10&nbsp;^&nbsp;5&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;9.&nbsp;No&nbsp;lead&nbsp;acetate&nbsp;solution&nbsp;is&nbsp;added&nbsp;in&nbsp;the&nbsp;sixth&nbsp;tube,&nbsp;and&nbsp;the&nbsp;standard&nbsp;is&nbsp;s4l&nbsp;0&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;10.&nbsp;Take&nbsp;400ul&nbsp;s4l&nbsp;10&nbsp;^&nbsp;1&nbsp;7.30&nbsp;solution&nbsp;and&nbsp;add&nbsp;it&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;tube,&nbsp;which&nbsp;is&nbsp;marked&nbsp;as&nbsp;s&nbsp;10&nbsp;^&nbsp;1&nbsp;0h&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">11.&nbsp;Take&nbsp;400ul&nbsp;s4l&nbsp;10&nbsp;^&nbsp;2&nbsp;7.30&nbsp;solution&nbsp;and&nbsp;add&nbsp;it&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;tube,&nbsp;which&nbsp;is&nbsp;marked&nbsp;as&nbsp;s&nbsp;10&nbsp;^&nbsp;2&nbsp;0h&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">12.&nbsp;Take&nbsp;400ul&nbsp;s4l&nbsp;10&nbsp;^&nbsp;3&nbsp;7.30&nbsp;solution&nbsp;and&nbsp;add&nbsp;it&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;tube,&nbsp;which&nbsp;is&nbsp;marked&nbsp;as&nbsp;s&nbsp;10&nbsp;^&nbsp;3&nbsp;0h&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">13.&nbsp;Take&nbsp;400ul&nbsp;s4l&nbsp;10&nbsp;^&nbsp;4&nbsp;7.30&nbsp;solution&nbsp;and&nbsp;add&nbsp;it&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;tube,&nbsp;which&nbsp;is&nbsp;marked&nbsp;as&nbsp;s&nbsp;10&nbsp;^&nbsp;4&nbsp;0h&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">14.&nbsp;Take&nbsp;400ul&nbsp;s4l&nbsp;10&nbsp;^&nbsp;5&nbsp;7.30&nbsp;solution&nbsp;and&nbsp;add&nbsp;it&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;tube,&nbsp;which&nbsp;is&nbsp;marked&nbsp;as&nbsp;s&nbsp;10&nbsp;^&nbsp;5&nbsp;0h&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">15.&nbsp;Take&nbsp;400ul&nbsp;s4l&nbsp;0&nbsp;7.30&nbsp;solution,&nbsp;add&nbsp;it&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;tube,&nbsp;and&nbsp;mark&nbsp;it&nbsp;as&nbsp;s00h&nbsp;7.30.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;16.&nbsp;Put&nbsp;all&nbsp;EP&nbsp;pipes&nbsp;into&nbsp;4&nbsp;℃&nbsp;for&nbsp;storage.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">17.&nbsp;Repeat&nbsp;the&nbsp;above&nbsp;steps&nbsp;for&nbsp;m2l&nbsp;and&nbsp;w5l.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">18.&nbsp;After&nbsp;that,&nbsp;400ul&nbsp;of&nbsp;each&nbsp;plastic&nbsp;pipe&nbsp;shall&nbsp;be&nbsp;sampled&nbsp;every&nbsp;2&nbsp;hours&nbsp;and&nbsp;put&nbsp;into&nbsp;1.5ml&nbsp;EP&nbsp;pipe&nbsp;and&nbsp;marked&nbsp;accordingly&nbsp;(for&nbsp;example,&nbsp;the&nbsp;sample&nbsp;with&nbsp;the&nbsp;date&nbsp;of&nbsp;7.30&nbsp;on&nbsp;the&nbsp;same&nbsp;day&nbsp;and&nbsp;swaying&nbsp;for&nbsp;2h&nbsp;in&nbsp;s4l&nbsp;10&nbsp;^&nbsp;1&nbsp;shall&nbsp;be&nbsp;marked&nbsp;with&nbsp;s&nbsp;10&nbsp;^&nbsp;1&nbsp;2H&nbsp;7.30)&nbsp;until&nbsp;8&nbsp;hours.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">19.&nbsp;Take&nbsp;three&nbsp;100ul&nbsp;samples&nbsp;from&nbsp;all&nbsp;samples&nbsp;and&nbsp;add&nbsp;them&nbsp;into&nbsp;the&nbsp;enzyme&nbsp;standard&nbsp;plate&nbsp;to&nbsp;measure&nbsp;their&nbsp;OD&nbsp;value.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span>
+</p>
+<p style="text-align: left;">
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">According&nbsp;to&nbsp;the&nbsp;OD&nbsp;value,&nbsp;the&nbsp;growth&nbsp;curve&nbsp;of&nbsp;bacteria&nbsp;was&nbsp;drawn&nbsp;under&nbsp;different&nbsp;lead&nbsp;concentration.</span>
+</p>
+<p>
+    <span style="font-size: 16px; color: rgb(0, 0, 0); font-family: 微软雅黑, &quot;Microsoft YaHei&quot;;">&nbsp;</span>
+</p>
-. Take three 100ul samples from all samples and add them into the enzyme standard plate to measure their OD value.
-According to the OD value, the growth curve of bacteria was drawn under different lead concentration.
@@ Line 1,023: / Line 1,491: @@
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