Difference between revisions of "Team:Vilnius-Lithuania/Parts"

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     font-weight: bold;
 
     font-weight: bold;
 
}
 
}
 +
.content-block a{
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color:#f6cd61!important;
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}
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.cover{
 +
margin-top:-18px;
 +
}
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td{
 +
vertical-align:center!important;
 +
}
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  .team-member-container{
 +
      position: relative;
 +
  }
 +
  .team-member{
 +
      margin-bottom:32px;
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 +
  .team-member img{
 +
    border-radius: 50%;
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    border: 2px solid #083c4c;
 +
    transition: all 0.5s ease;
 +
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 +
  .team-member:hover img{
 +
    /* box-shadow: 0 0 10px 10px #083c4c; */
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    border: 10px solid #083c4c;
 +
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  .team-member-middle {
 +
    transition: .5s ease;
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 +
    -ms-transform: translate(-50%, -50%);
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    text-align: center!important;
 +
  }
 +
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  .team-member-container:hover .team-member-middle {
 +
    opacity: 1;
 +
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 +
  .team-member-text h3{
 +
    font-family: 'bebas';
 +
    color: #f6cd61;
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    font-size:2rem;
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    text-shadow: 0px 0px 10px #fbdd90;
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    font-weight: 400;
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 +
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  .team-member-text p{
 +
    color: white;
 +
text-align:center!important;
 +
  }
 
<style>
 
<style>
 
/*!
 
/*!
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}
 
}
−
p, ul, table{
+
ul, table{
 
     font-family: 'intro', sans-serif!important;
 
     font-family: 'intro', sans-serif!important;
 
margin: 0!important;
 
margin: 0!important;
 +
}
 +
p{
 +
    font-family: 'intro', sans-serif!important;
 
}
 
}
 
img{
 
img{
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table{
 
table{
 
     width: 100%;
 
     width: 100%;
−
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     background-color: #010C11;
 
     color: #bdbdbd;
 
     color: #bdbdbd;
 
}
 
}
 
th,td{
 
th,td{
 
     padding:12px;
 
     padding:12px;
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     border-top:1px solid #010C11;
 
}
 
}
 
h1{
 
h1{
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             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Model" class="sub">Overview</a>
 
             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Model" class="sub">Overview</a>
 
             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control_Model" class="sub">Post-translational regulation</a>
 
             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control_Model" class="sub">Post-translational regulation</a>
−
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Plasmid_Copy_Number_Control_Model" class="sub">Plasmid copy number control</a>
+
 
 
             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation_CarH" class="sub">Transcriptional regulation (CarH)</a>
 
             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation_CarH" class="sub">Transcriptional regulation (CarH)</a>
−
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradataion_Rate_Control_BpHP1" class="sub">Transcriptional regulation (BphP1)</a>
+
 
 
         <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Software">Software</a>
 
         <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Software">Software</a>
 
         <a href="#">Human Practices</a>
 
         <a href="#">Human Practices</a>
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                 <li class="menu-item" style="position: relative;">
 
                 <li class="menu-item" style="position: relative;">
 
                     <a class="active" href="https://2019.igem.org/Team:Vilnius-Lithuania/Parts">Parts</a>
 
                     <a class="active" href="https://2019.igem.org/Team:Vilnius-Lithuania/Parts">Parts</a>
 +
                </li>
 +
                <li class="menu-item" style="position: relative;">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Measurement">Measurement</a>
 
                 </li>
 
                 </li>
 
             </ul>
 
             </ul>
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                     <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                     <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                       <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control_Model">Post-translational regulation</a>
 
                       <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control_Model">Post-translational regulation</a>
−
                    </li>
 
−
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
−
                      <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Plasmid_Copy_Number_Control_Model">Plasmid copy number control</a>
 
 
                     </li>
 
                     </li>
 
                     <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                     <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                       <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation_CarH">Transcriptional regulation (CarH)</a>
 
                       <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation_CarH">Transcriptional regulation (CarH)</a>
−
                    </li>
 
−
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
−
                      <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradataion_Rate_Control_BpHP1">Transcriptional regulation (BphP1) </a>
 
 
                     </li>
 
                     </li>
  
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                 <div class="content-block">
 
                 <div class="content-block">
 
                     <!--TEKSTAS-->
 
                     <!--TEKSTAS-->
−
                    <h4 class="page-heading">First heading</h4>
+
<h4 class="page-heading">Overview</h4>
−
                    <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur congue magna magna, ac gravida
+
<p>Until this moment, bacterial optogenetic regulation was only possible at the transcriptional level. However, the existing optogenetic controllers, even with reasonable control, all the transcriptional regulators have an imperfect design.</p>
−
                        urna lacinia in. Duis eleifend suscipit nisi non maximus. Orci varius natoque penatibus et magnis
+
 
−
                        dis parturient montes, nascetur ridiculus mus. Integer sed arcu tortor. Sed ut mi ac quam luctus
+
<p>Vilnius-Lithuania iGEM 2019 team created a collection that consists of parts that allow three-level modular control with light in bacteria.</p>
−
                         iaculis id vitae magna. Praesent ultricies lacus id pharetra viverra. Nulla lobortis bibendum augue,
+
 
−
                        et congue ante elementum at. Mauris ante odio, porttitor vel varius malesuada, porta ac neque.</p>
+
<p>Modular transcription regulators CarH and Q-Pas1 are chimeric proteins that open up a possibility for researchers to control bacteria function at the transcriptional level with green and near-infrared (NIR) light.  
−
               
+
 
−
               
+
<p>Light-inducible regulation system for the plasmid copy number enables to maintain a low plasmid copy number at the growth stage and increase it for the production stage.</p>
 +
 
 +
<p>Finally, the post-translational control system works by increasing the POI's degradation rate and thus allows new possibilities to control bacterial dynamics at the post-translational level with rapid kinetics.</p>
 +
<br><br>
 +
<div class="row">
 +
 
 +
<div class="col-4 team-member">
 +
<div class="team-member-container">
 +
                    <img src="https://2019.igem.org/wiki/images/e/e1/T--Vilnius-Lithuania--Parts-1.png">
 +
                    <div class="team-member-middle">
 +
                         <div class="team-member-text">
 +
                            <a href="#basic"><h3>Basic Parts</h3></a>
 +
 
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
   
 +
</div>
 +
<div class="col-4 team-member">
 +
<div class="team-member-container">
 +
                    <img src="https://2019.igem.org/wiki/images/0/09/T--Vilnius-Lithuania--Parts-2.png">
 +
                    <div class="team-member-middle">
 +
                        <div class="team-member-text">
 +
                            <a href="#composite"><h3>Composite Parts</h3></a>
 +
 
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
   
 +
</div>
 +
 +
<div class="col-4 team-member">
 +
<div class="team-member-container">
 +
                    <img src="https://2019.igem.org/wiki/images/9/9a/T--Vilnius-Lithuania--Parts-3.png">
 +
                    <div class="team-member-middle">
 +
                        <div class="team-member-text">
 +
                            <a href="#collection"><h3>Collection Parts</h3></a>
 +
 
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
   
 +
</div> 
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
<h4 class="page-heading" id="basic">Basic Parts</h4><br>
 +
 
 +
<table style="width:100%;text-align:center">
 +
  <tr>
 +
    <th style="width:15%">Part Name</th>
 +
    <th style="width:15%">Type</th>
 +
    <th style="width:70%">Short Description</th>
 +
  </tr>
 +
  <tbody>
 +
 
 +
    <tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032000">BBa_K3032000</a></td><td>Tag</td><td>Light-inducible protein degradation tag (159AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032001">BBa_K3032001</a></td><td>Tag</td><td>Light-inducible protein degradation tag (160AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032002">BBa_K3032002</a></td><td>Tag</td><td>Light-inducible protein degradation tag (161AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032003">BBa_K3032003</a></td><td>Tag</td><td>Light-inducible protein degradation tag (162AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032004">BBa_K3032004</a></td><td>Tag</td><td>Light-inducible protein degradation tag (163AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032005">BBa_K3032005</a></td><td>Tag</td><td>Light-inducible protein degradation tag (164AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032006">BBa_K3032006</a></td><td>Tag</td><td>Light-inducible protein degradation tag (165AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032007">BBa_K3032007</a></td><td>Tag</td><td>Light-inducible protein degradation tag (166AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032008">BBa_K3032008</a></td><td>Tag</td><td>Light-inducible protein degradation tag (167AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032009">BBa_K3032009</a></td><td>Tag</td><td>Light-inducible protein degradation tag (168AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032010">BBa_K3032010</a></td><td>Tag</td><td>Light-inducible protein degradation tag (169AtLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032011">BBa_K3032011</a></td><td>Tag</td><td>Light-inducible protein degradation tag (144AsLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032012">BBa_K3032012</a></td><td>Tag</td><td>Light-inducible protein degradation tag (139AsLOV2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032013">BBa_K3032013</a></td><td>Coding</td><td>CarH repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032014">BBa_K3032014</a></td><td>Regulatory</td><td>CarH promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032015">BBa_K3032015</a></td><td>Coding</td><td>ho1 (heme oxygenase 1)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032016">BBa_K3032016</a></td><td>Coding</td><td>BphP1 (bacterial phytochrome photoreceptor)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032017">BBa_K3032017</a></td><td>Coding</td><td>Gal4_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032018">BBa_K3032018</a></td><td>Coding</td><td>LexA75_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032019">BBa_K3032019</a></td><td>Coding</td><td>LexA90_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032020">BBa_K3032020</a></td><td>Coding</td><td>LexA91_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032021">BBa_K3032021</a></td><td>Coding</td><td>LexA92_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032022">BBa_K3032022</a></td><td>Coding</td><td>LexA93_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032023">BBa_K3032023</a></td><td>Coding</td><td>LexA94_Q-PAS1 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032024">BBa_K3032024</a></td><td>Coding</td><td>PpsR2 repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032025">BBa_K3032025</a></td><td>Regulatory</td><td>consensus LexA promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032026">BBa_K3032026</a></td><td>Regulatory</td><td>strong LexA promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032027">BBa_K3032027</a></td><td>Regulatory</td><td>cUAS Gal4 promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032028">BBa_K3032028</a></td><td>Regulatory</td><td>cUAS 3A Gal4 promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032029">BBa_K3032029</a></td><td>Regulatory</td><td>ColE based strong LexA promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032030">BBa_K3032030</a></td><td>Regulatory</td><td>ColE based cUAS Gal4  promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032031">BBa_K3032031</a></td><td>Regulatory</td><td>T7 based strong LexA promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032032">BBa_K3032032</a></td><td>Regulatory</td><td>T7 based cUAS Gal4 promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032033">BBa_K3032033</a></td><td>Regulatory</td><td>ColE based PpsR2 promoter (1)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032034">BBa_K3032034</a></td><td>Regulatory</td><td>ColE based PpsR2 promoter (2)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032035">BBa_K3032035</a></td><td>Regulatory</td><td>pBR_crtE promoter</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032036">BBa_K3032036</a></td><td>Coding</td><td>BLUF-cI repressor</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032110">BBa_K3032110</a></td><td>Regulatory</td><td>EL222 regulated promoter based on Anderson (0.86)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032111">BBa_K3032111</a></td><td>Regulatory</td><td>EL222 regulated promoter based on Anderson (0.47)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032112">BBa_K3032112</a></td><td>Regulatory</td><td>EL222 regulated promoter based on Anderson (0.16)</td></tr>
 +
    </tbody>
 +
</table>
 +
<br>
 +
 
 +
<h4 class="page-heading" id="composite">Composite Parts</h4>
 +
<br>
 +
<table style="width:100%;text-align:center">
 +
  <tr>
 +
    <th style="width:15%">Part Name</th>
 +
    <th style="width:15%">Type</th>
 +
    <th style="width:70%">Short Description</th>
 +
  </tr>
 +
  <tbody>
 +
 
 +
    <tr>
 +
   
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032050">BBa_K3032050</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (159AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032051">BBa_K3032051</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (160AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032052">BBa_K3032052</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (161AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032053">BBa_K3032053</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (162AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032054">BBa_K3032054</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (163AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032055">BBa_K3032055</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (164AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032056">BBa_K3032056</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (165AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032057">BBa_K3032057</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (166AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032058">BBa_K3032058</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (167AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032059">BBa_K3032059</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (168AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032060">BBa_K3032060</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (169AtLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032061">BBa_K3032061</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (144AsLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032062">BBa_K3032062</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (139AsLOV2, Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032070">BBa_K3032070</a></td><td>Composite</td><td>mRFP1 reporter under light-inducible CarH promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032071">BBa_K3032071</a></td><td>Composite</td><td>CarH repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032072">BBa_K3032072</a></td><td>Composite</td><td>CarH repressor under medium Anderson (0.33) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032073">BBa_K3032073</a></td><td>Composite</td><td>CarH repressor under weak Anderson (0.1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032074">BBa_K3032074</a></td><td>Device</td><td>Ready to use mRFP1-CarH device (strong)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032075">BBa_K3032075</a></td><td>Device</td><td>Ready to use mRFP1-CarH device (medium)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032076">BBa_K3032076</a></td><td>Device</td><td>Ready to use mRFP1-CarH device (weak)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032080">BBa_K3032080</a></td><td>Composite</td><td>ho1 under medium Anderson (0.33) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032081">BBa_K3032081</a></td><td>Composite</td><td>BphP1 under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032082">BBa_K3032082</a></td><td>Composite</td><td>Gal4_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032083">BBa_K3032083</a></td><td>Composite</td><td>LexA75_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032084">BBa_K3032084</a></td><td>Composite</td><td>LexA90_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032085">BBa_K3032085</a></td><td>Composite</td><td>LexA91_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032086">BBa_K3032086</a></td><td>Composite</td><td>LexA92_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032087">BBa_K3032087</a></td><td>Composite</td><td>LexA93_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032088">BBa_K3032088</a></td><td>Composite</td><td>LexA94_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032089">BBa_K3032089</a></td><td>Composite</td><td>PpsR2 repressor under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032090">BBa_K3032090</a></td><td>Composite</td><td>sfGFP reporter under light-inducible consensus LexA promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032091">BBa_K3032091</a></td><td>Composite</td><td>sfGFP reporter under light-inducible strong LexA promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032092">BBa_K3032092</a></td><td>Composite</td><td>sfGFP reporter under light-inducible cUAS Gal4 promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032093">BBa_K3032093</a></td><td>Composite</td><td>sfGFP reporter under light-inducible cUAS 3A Gal4  promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032094">BBa_K3032094</a></td><td>Composite</td><td>sfGFP reporter under light-inducible ColE-LexA promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032095">BBa_K3032095</a></td><td>Composite</td><td>sfGFP reporter under light-inducible ColE-Gal4 promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032096">BBa_K3032096</a></td><td>Composite</td><td>sfGFP reporter under light-inducible T7-LexA promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032097">BBa_K3032097</a></td><td>Composite</td><td>sfGFP reporter under light-inducible T7-Gal4 promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032098">BBa_K3032098</a></td><td>Composite</td><td>sfGFP reporter under light-inducible ColE-PpsR2 promoter (1) (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032099">BBa_K3032099</a></td><td>Composite</td><td>sfGFP reporter under light-inducible ColE-PpsR2 promoter (2) (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032100">BBa_K3032100</a></td><td>Composite</td><td>sfGFP reporter under light-inducible pBR_crtE promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032113">BBa_K3032113</a></td><td>Composite</td><td>EL222 protein under strong Anderson (1) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032114">BBa_K3032114</a></td><td>Composite</td><td>EL222 protein under medium Anderson (0.47) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032115">BBa_K3032115</a></td><td>Composite</td><td>EL222 protein under weak Anderson (0.16) promoter (Ready for Expression)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032116">BBa_K3032116</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16) with mRFP1 (0.16)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032117">BBa_K3032117</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16) with mRFP1 (0.24)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032118">BBa_K3032118</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16) with mRFP1 (0.33)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032119">BBa_K3032119</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.47) with mRFP1 (0.16)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032120">BBa_K3032120</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.47) with mRFP1 (0.24)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032121">BBa_K3032121</a></td><td>Composite</td><td> Light-regulated plasmid copy number device (0.47) with mRFP1 (0.33)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032122">BBa_K3032122</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86) with mRFP1 (0.16)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032123">BBa_K3032123</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86) with mRFP1 (0.24)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032124">BBa_K3032124</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86) with mRFP1 (0.33)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032125">BBa_K3032125</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032126">BBa_K3032126</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.47)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032127">BBa_K3032127</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032128">BBa_K3032128</a></td><td>Intermediate</td><td>Light-regulated plasmid copy number device intermediate (0.86)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032129">BBa_K3032129</a></td><td>Intermediate</td><td>Light-regulated plasmid copy number device intermediate (0.47)</td></tr>
 +
   
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032130">BBa_K3032130</a></td><td>Intermediate</td><td>Light-regulated plasmid copy number device intermediate (0.16)</td></tr>
 +
    </tbody>
 +
</table>
 +
<br>
 +
 
 +
<h4 class="page-heading" id="collection">Part Collection</h4>
 +
<br>
 +
<table style="width:100%;text-align:center">
 +
  <tr>
 +
    <th style="width:15%">Part Name</th>
 +
    <th style="width:15%">Type</th>
 +
    <th style="width:70%">Short Description</th>
 +
  </tr>
 +
  <tbody>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032010">BBa_K3032010</a></td><td>Tag</td><td>Light-inducible protein degradation tag (169AtLOV2)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032060">BBa_K3032060</a></td><td>Composite</td><td>sfGFP tagged with light-inducible protein degradation tag (169AtLOV2, Ready for Expression)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032013">BBa_K3032013</a></td><td>Coding</td><td>CarH repressor</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032014">BBa_K3032014</a></td><td>Regulatory</td><td>CarH promoter</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032128">BBa_K3032128</a></td><td>Intermediate</td><td>Light-regulated plasmid copy number device intermediate (0.86)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032129">BBa_K3032129</a></td><td>Intermediate</td><td>Light-regulated plasmid copy number device intermediate (0.47)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032130">BBa_K3032130</a></td><td>Intermediate</td><td>Light-regulated plasmid copy number device intermediate (0.16)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032116">BBa_K3032116</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16) with mRFP1 (0.16)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032117">BBa_K3032117</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16) with mRFP1 (0.24)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032118">BBa_K3032118</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.16) with mRFP1 (0.33)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032119">BBa_K3032119</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.47) with mRFP1 (0.16)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032120">BBa_K3032120</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.47) with mRFP1 (0.24)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032121">BBa_K3032121</a></td><td>Composite</td><td> Light-regulated plasmid copy number device (0.47) with mRFP1 (0.33)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032122">BBa_K3032122</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86) with mRFP1 (0.16)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032123">BBa_K3032123</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86) with mRFP1 (0.24)</td></tr>
 +
        <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032124">BBa_K3032124</a></td><td>Composite</td><td>Light-regulated plasmid copy number device (0.86) with mRFP1 (0.33)</td></tr>
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032074">BBa_K3032074</a></td><td>Device</td><td>Ready to use mRFP1-CarH device (strong)</td></tr>
 +
    <tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3032076">BBa_K3032076</a></td><td>Device</td><td>Ready to use mRFP1-CarH device (weak)</td></tr>
 +
       
 +
 
 +
    </tbody>
 +
</table>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>

Latest revision as of 02:18, 22 October 2019

Parts

Overview

Until this moment, bacterial optogenetic regulation was only possible at the transcriptional level. However, the existing optogenetic controllers, even with reasonable control, all the transcriptional regulators have an imperfect design.

Vilnius-Lithuania iGEM 2019 team created a collection that consists of parts that allow three-level modular control with light in bacteria.

Modular transcription regulators CarH and Q-Pas1 are chimeric proteins that open up a possibility for researchers to control bacteria function at the transcriptional level with green and near-infrared (NIR) light.

Light-inducible regulation system for the plasmid copy number enables to maintain a low plasmid copy number at the growth stage and increase it for the production stage.

Finally, the post-translational control system works by increasing the POI's degradation rate and thus allows new possibilities to control bacterial dynamics at the post-translational level with rapid kinetics.



Basic Parts

Composite Parts

Collection Parts


Basic Parts


Part Name Type Short Description
BBa_K3032000TagLight-inducible protein degradation tag (159AtLOV2)
BBa_K3032001TagLight-inducible protein degradation tag (160AtLOV2)
BBa_K3032002TagLight-inducible protein degradation tag (161AtLOV2)
BBa_K3032003TagLight-inducible protein degradation tag (162AtLOV2)
BBa_K3032004TagLight-inducible protein degradation tag (163AtLOV2)
BBa_K3032005TagLight-inducible protein degradation tag (164AtLOV2)
BBa_K3032006TagLight-inducible protein degradation tag (165AtLOV2)
BBa_K3032007TagLight-inducible protein degradation tag (166AtLOV2)
BBa_K3032008TagLight-inducible protein degradation tag (167AtLOV2)
BBa_K3032009TagLight-inducible protein degradation tag (168AtLOV2)
BBa_K3032010TagLight-inducible protein degradation tag (169AtLOV2)
BBa_K3032011TagLight-inducible protein degradation tag (144AsLOV2)
BBa_K3032012TagLight-inducible protein degradation tag (139AsLOV2)
BBa_K3032013CodingCarH repressor
BBa_K3032014RegulatoryCarH promoter
BBa_K3032015Codingho1 (heme oxygenase 1)
BBa_K3032016CodingBphP1 (bacterial phytochrome photoreceptor)
BBa_K3032017CodingGal4_Q-PAS1 repressor
BBa_K3032018CodingLexA75_Q-PAS1 repressor
BBa_K3032019CodingLexA90_Q-PAS1 repressor
BBa_K3032020CodingLexA91_Q-PAS1 repressor
BBa_K3032021CodingLexA92_Q-PAS1 repressor
BBa_K3032022CodingLexA93_Q-PAS1 repressor
BBa_K3032023CodingLexA94_Q-PAS1 repressor
BBa_K3032024CodingPpsR2 repressor
BBa_K3032025Regulatoryconsensus LexA promoter
BBa_K3032026Regulatorystrong LexA promoter
BBa_K3032027RegulatorycUAS Gal4 promoter
BBa_K3032028RegulatorycUAS 3A Gal4 promoter
BBa_K3032029RegulatoryColE based strong LexA promoter
BBa_K3032030RegulatoryColE based cUAS Gal4 promoter
BBa_K3032031RegulatoryT7 based strong LexA promoter
BBa_K3032032RegulatoryT7 based cUAS Gal4 promoter
BBa_K3032033RegulatoryColE based PpsR2 promoter (1)
BBa_K3032034RegulatoryColE based PpsR2 promoter (2)
BBa_K3032035RegulatorypBR_crtE promoter
BBa_K3032036CodingBLUF-cI repressor
BBa_K3032110RegulatoryEL222 regulated promoter based on Anderson (0.86)
BBa_K3032111RegulatoryEL222 regulated promoter based on Anderson (0.47)
BBa_K3032112RegulatoryEL222 regulated promoter based on Anderson (0.16)

Composite Parts


Part Name Type Short Description
BBa_K3032050CompositesfGFP tagged with light-inducible protein degradation tag (159AtLOV2, Ready for Expression)
BBa_K3032051CompositesfGFP tagged with light-inducible protein degradation tag (160AtLOV2, Ready for Expression)
BBa_K3032052CompositesfGFP tagged with light-inducible protein degradation tag (161AtLOV2, Ready for Expression)
BBa_K3032053CompositesfGFP tagged with light-inducible protein degradation tag (162AtLOV2, Ready for Expression)
BBa_K3032054CompositesfGFP tagged with light-inducible protein degradation tag (163AtLOV2, Ready for Expression)
BBa_K3032055CompositesfGFP tagged with light-inducible protein degradation tag (164AtLOV2, Ready for Expression)
BBa_K3032056CompositesfGFP tagged with light-inducible protein degradation tag (165AtLOV2, Ready for Expression)
BBa_K3032057CompositesfGFP tagged with light-inducible protein degradation tag (166AtLOV2, Ready for Expression)
BBa_K3032058CompositesfGFP tagged with light-inducible protein degradation tag (167AtLOV2, Ready for Expression)
BBa_K3032059CompositesfGFP tagged with light-inducible protein degradation tag (168AtLOV2, Ready for Expression)
BBa_K3032060CompositesfGFP tagged with light-inducible protein degradation tag (169AtLOV2, Ready for Expression)
BBa_K3032061CompositesfGFP tagged with light-inducible protein degradation tag (144AsLOV2, Ready for Expression)
BBa_K3032062CompositesfGFP tagged with light-inducible protein degradation tag (139AsLOV2, Ready for Expression)
BBa_K3032070CompositemRFP1 reporter under light-inducible CarH promoter (Ready for Expression)
BBa_K3032071CompositeCarH repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032072CompositeCarH repressor under medium Anderson (0.33) promoter (Ready for Expression)
BBa_K3032073CompositeCarH repressor under weak Anderson (0.1) promoter (Ready for Expression)
BBa_K3032074DeviceReady to use mRFP1-CarH device (strong)
BBa_K3032075DeviceReady to use mRFP1-CarH device (medium)
BBa_K3032076DeviceReady to use mRFP1-CarH device (weak)
BBa_K3032080Compositeho1 under medium Anderson (0.33) promoter (Ready for Expression)
BBa_K3032081CompositeBphP1 under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032082CompositeGal4_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032083CompositeLexA75_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032084CompositeLexA90_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032085CompositeLexA91_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032086CompositeLexA92_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032087CompositeLexA93_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032088CompositeLexA94_Q-PAS1 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032089CompositePpsR2 repressor under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032090CompositesfGFP reporter under light-inducible consensus LexA promoter (Ready for Expression)
BBa_K3032091CompositesfGFP reporter under light-inducible strong LexA promoter (Ready for Expression)
BBa_K3032092CompositesfGFP reporter under light-inducible cUAS Gal4 promoter (Ready for Expression)
BBa_K3032093CompositesfGFP reporter under light-inducible cUAS 3A Gal4 promoter (Ready for Expression)
BBa_K3032094CompositesfGFP reporter under light-inducible ColE-LexA promoter (Ready for Expression)
BBa_K3032095CompositesfGFP reporter under light-inducible ColE-Gal4 promoter (Ready for Expression)
BBa_K3032096CompositesfGFP reporter under light-inducible T7-LexA promoter (Ready for Expression)
BBa_K3032097CompositesfGFP reporter under light-inducible T7-Gal4 promoter (Ready for Expression)
BBa_K3032098CompositesfGFP reporter under light-inducible ColE-PpsR2 promoter (1) (Ready for Expression)
BBa_K3032099CompositesfGFP reporter under light-inducible ColE-PpsR2 promoter (2) (Ready for Expression)
BBa_K3032100CompositesfGFP reporter under light-inducible pBR_crtE promoter (Ready for Expression)
BBa_K3032113CompositeEL222 protein under strong Anderson (1) promoter (Ready for Expression)
BBa_K3032114CompositeEL222 protein under medium Anderson (0.47) promoter (Ready for Expression)
BBa_K3032115CompositeEL222 protein under weak Anderson (0.16) promoter (Ready for Expression)
BBa_K3032116CompositeLight-regulated plasmid copy number device (0.16) with mRFP1 (0.16)
BBa_K3032117CompositeLight-regulated plasmid copy number device (0.16) with mRFP1 (0.24)
BBa_K3032118CompositeLight-regulated plasmid copy number device (0.16) with mRFP1 (0.33)
BBa_K3032119CompositeLight-regulated plasmid copy number device (0.47) with mRFP1 (0.16)
BBa_K3032120CompositeLight-regulated plasmid copy number device (0.47) with mRFP1 (0.24)
BBa_K3032121Composite Light-regulated plasmid copy number device (0.47) with mRFP1 (0.33)
BBa_K3032122CompositeLight-regulated plasmid copy number device (0.86) with mRFP1 (0.16)
BBa_K3032123CompositeLight-regulated plasmid copy number device (0.86) with mRFP1 (0.24)
BBa_K3032124CompositeLight-regulated plasmid copy number device (0.86) with mRFP1 (0.33)
BBa_K3032125CompositeLight-regulated plasmid copy number device (0.16)
BBa_K3032126CompositeLight-regulated plasmid copy number device (0.47)
BBa_K3032127CompositeLight-regulated plasmid copy number device (0.86)
BBa_K3032128IntermediateLight-regulated plasmid copy number device intermediate (0.86)
BBa_K3032129IntermediateLight-regulated plasmid copy number device intermediate (0.47)
BBa_K3032130IntermediateLight-regulated plasmid copy number device intermediate (0.16)

Part Collection


Part Name Type Short Description
BBa_K3032010TagLight-inducible protein degradation tag (169AtLOV2)
BBa_K3032060CompositesfGFP tagged with light-inducible protein degradation tag (169AtLOV2, Ready for Expression)
BBa_K3032013CodingCarH repressor
BBa_K3032014RegulatoryCarH promoter
BBa_K3032128IntermediateLight-regulated plasmid copy number device intermediate (0.86)
BBa_K3032129IntermediateLight-regulated plasmid copy number device intermediate (0.47)
BBa_K3032130IntermediateLight-regulated plasmid copy number device intermediate (0.16)
BBa_K3032116CompositeLight-regulated plasmid copy number device (0.16) with mRFP1 (0.16)
BBa_K3032117CompositeLight-regulated plasmid copy number device (0.16) with mRFP1 (0.24)
BBa_K3032118CompositeLight-regulated plasmid copy number device (0.16) with mRFP1 (0.33)
BBa_K3032119CompositeLight-regulated plasmid copy number device (0.47) with mRFP1 (0.16)
BBa_K3032120CompositeLight-regulated plasmid copy number device (0.47) with mRFP1 (0.24)
BBa_K3032121Composite Light-regulated plasmid copy number device (0.47) with mRFP1 (0.33)
BBa_K3032122CompositeLight-regulated plasmid copy number device (0.86) with mRFP1 (0.16)
BBa_K3032123CompositeLight-regulated plasmid copy number device (0.86) with mRFP1 (0.24)
BBa_K3032124CompositeLight-regulated plasmid copy number device (0.86) with mRFP1 (0.33)
BBa_K3032074DeviceReady to use mRFP1-CarH device (strong)
BBa_K3032076DeviceReady to use mRFP1-CarH device (weak)
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