Difference between revisions of "Team:Vilnius-Lithuania"

m
 
(47 intermediate revisions by 2 users not shown)
Line 25: Line 25:
 
#globalWrapper{
 
#globalWrapper{
 
font-size:100%;
 
font-size:100%;
 +
}
 +
 +
.menu-item-bullet::before{
 +
    content: "-";
 +
    color: #bdbdbd;
 +
    display: inline-block;
 +
    margin-left: -8px;
 +
    font-weight: bold;
 
}
 
}
 
<style>
 
<style>
Line 47: Line 55:
 
@font-face {
 
@font-face {
 
     font-family: montserrat;
 
     font-family: montserrat;
−
     src: url('https://2019.igem.org/wiki/images/7/7b/T--Vilnius-Lithuania--montserrat.otf');
+
     src: url('https://2019.igem.org/wiki/images/0/0a/T--Vilnius-Lithuania--mont.ttf');
 
}
 
}
 
body{
 
body{
Line 136: Line 144:
 
.menu-item{
 
.menu-item{
 
     padding: 16px 8px;
 
     padding: 16px 8px;
 +
transition: margin-left 0.25s ease;
 
}
 
}
 
.menu-item-sub::after{
 
.menu-item-sub::after{
Line 158: Line 167:
 
     text-decoration: none;
 
     text-decoration: none;
 
     color: #f6cd61;
 
     color: #f6cd61;
−
    padding-left:8px;
+
}
 +
.menu-item:hover{
 +
 
 +
margin-left:8px
 +
 
 
}
 
}
 
.menu-item:hover:after{
 
.menu-item:hover:after{
Line 208: Line 221:
 
     margin: 72px 0;
 
     margin: 72px 0;
 
     z-index:2;
 
     z-index:2;
−
 
−
 
−
 
 
}
 
}
 
.main-container:hover{
 
.main-container:hover{
Line 254: Line 264:
 
     text-shadow: 0px 0px 10px #f6cd61;
 
     text-shadow: 0px 0px 10px #f6cd61;
 
text-transform: uppercase;
 
text-transform: uppercase;
 +
}
 +
.text a {
 +
    font-family: 'montserrat'!important;
 +
    color: #f6cd61;
 +
    text-decoration:none;
 +
    font-size: 1.5rem;
 +
    float: right;
 +
    margin-bottom: 5%;
 +
}
 +
.text a:hover {
 +
  color: #ffe28c;
 
}
 
}
 
.text{
 
.text{
Line 346: Line 367:
 
.topnav {
 
.topnav {
 
     overflow: hidden;
 
     overflow: hidden;
−
     background-color: #083c4c;
+
     background-color: #010C11;
 +
position:relative;
 +
z-index:10;
 
   }
 
   }
 
    
 
    
Line 404: Line 427:
 
       color:black;
 
       color:black;
 
   }
 
   }
 +
.text h1{
 +
    font-size:32px!important;
 +
}
 +
.logo-video{
 +
height:200px!important;
 +
}
 
    
 
    
 
}
 
}
 +
  
 
/* When the screen is less than 600 pixels wide, hide all links, except for the first one ("Home"). Show the link that contains should open and close the topnav (.icon) */
 
/* When the screen is less than 600 pixels wide, hide all links, except for the first one ("Home"). Show the link that contains should open and close the topnav (.icon) */
Line 434: Line 464:
 
   }
 
   }
  
−
 
+
@media (min-width: 768px) and (max-width: 991.98px) {
−
.laser{
+
.logo-video{
−
    width:50%;
+
height:500px!important;
−
    height:1px;
+
}
−
    position:fixed;
+
−
    background-color: #f6cd61;
+
−
    top:50%;
+
−
    z-index:-1;
+
−
    border-radius: 50%;
+
−
    transition: all 0.7s ease;
+
−
    /* border:2px solid #f6cd61; */
+
−
    opacity: 0.25;
+
−
    box-shadow: 0 0 1px 1px #f6cd61;
+
−
    -webkit-animation-name: laserAnimation;
+
−
    -webkit-animation-duration: 1800ms;
+
−
    animation-name: laserAnimation;
+
−
    animation-duration: 1800ms;
+
−
    animation-iteration-count: infinite;
+
−
 
+
−
 
+
 
}
 
}
−
 
−
@-webkit-keyframes laserAnimation {
 
−
    0%  {width: 0;left:0;}
 
−
    50%  {width: 50%;left: 50px;}
 
−
    100% {width: 0;left:0;}
 
−
  }
 
−
 
 
−
  @keyframes laserAnimation {
 
−
    0%  {width: 0;left:0;}
 
−
    50%  {width: 50%;left: 50%;}
 
−
    100% {width: 0;left:0;}
 
−
  }
 
  
 
   #overlay {
 
   #overlay {
Line 482: Line 484:
 
   }
 
   }
  
−
  @keyframes clickAnimation {
 
−
    0%  {width: 0;height:0;opacity:0}
 
−
    50%  {width: 0;height:0;opacity:0.5}
 
−
    100% {width: 1000px;height:1000px;opacity:0}
 
−
  }
 
  
 
    
 
    
Line 502: Line 499:
  
 
<div class="topnav d-sm-block d-md-none" id="Topnav">
 
<div class="topnav d-sm-block d-md-none" id="Topnav">
−
                <a href="#home" class="active">MENU</a>
+
        <a href="#home" class="active">MENU</a>
−
                <a href="#">Home</a>
+
        <a href="https://2019.igem.org/Team:Vilnius-Lithuania">Home</a>
−
                <a href="#">Project</a>
+
        <a href="#">Project</a>
−
                    <a href="#" class="sub">Inspiration & Description</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Description" class="sub">Inspiration & Description</a>
−
                    <a href="#" class="sub">Design & Results</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation" class="sub">Transcriptional regulation</a>
−
                    <a href="#" class="sub">Demonstration</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Plasmid_Copy_Number_Control" class="sub">Plasmid copy number control</a>
−
                    <a href="#" class="sub">Parts</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control" class="sub">Post-translational regulation</a>
−
                <a href="#">Modeling</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Demonstrate" class="sub">Demonstration</a>
−
                <a href="#">Software</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Parts" class="sub">Parts</a>
−
                <a href="#">Human Practices</a>
+
        <a href="#">Modeling</a>
−
                    <a href="#" class="sub">HP</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Model" class="sub">Overview</a>
−
                    <a href="#" class="sub">HP model</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control_Model" class="sub">Post-translational regulation</a>
−
                    <a href="#" class="sub">Integrated</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation_CarH" class="sub">Transcriptional regulation (CarH)</a>
−
                    <a href="#" class="sub">Collaboration</a>
+
        <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Software">Software</a>
−
                <a href="#">Team</a>
+
        <a href="#">Human Practices</a>
−
                    <a href="#">Members</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Human_Practices" class="sub">Integrated Human Practices</a>
−
                    <a href="#">Attributions</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Public_Engagement" class="sub">Education & Public Engagment</a>
−
                    <a href="#">Sponsors</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Collaborations" class="sub">Collaboration</a>
−
                <a href="#">Notebook</a>
+
        <a href="#">Team</a>
−
                    <a href="#">Lab journal</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Team" class="sub">Members</a>
−
                    <a href="#">Protocols</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Attributions" class="sub">Attributions</a>
−
                    <a href="#">Safety</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Sponsors" class="sub">Sponsors</a>
−
                <a href="javascript:void(0);" class="icon" onclick="extendMobileMenu()">
+
        <a href="#">Notebook</a>
−
                    &#9776;
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Notebook" class="sub">Lab journal</a>
−
                </a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Protocols" class="sub">Protocols</a>
−
            </div>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Safety" class="sub">Safety</a>
 +
        <a href="javascript:void(0);" class="icon" onclick="extendMobileMenu()">
 +
            &#9776;
 +
        </a>
 +
    </div>
 
<div class="sidenav">
 
<div class="sidenav">
−
        <div class="colight"><img src="https://2019.igem.org/wiki/images/6/68/T--Vilnius-Lithuania--colight.png"></div>
+
<div class="colight"><a href="https://2019.igem.org/Team:Vilnius-Lithuania"><img src="https://2019.igem.org/wiki/images/6/68/T--Vilnius-Lithuania--colight.png"></a></div>
−
       
+
−
        <div class="menu-container">
+
  
 +
<div class="menu-container">
  
−
                <ul class="menu sub-menu-project sub-menu">
+
 
 +
        <ul class="menu sub-menu-project sub-menu">
 +
                <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                    <a href="#" class="return return-project" onclick="returnMenu('project')">Back to Main Menu</a>
 +
                </li>
 +
                <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Description">Inspiration & Description</a>
 +
                </li>
 +
                <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                    <a href="#">Design & Results:</a>
 +
                </li>
 +
                <li class="menu-item menu-item-bullet" style="position: relative;left:200px;opacity:0;padding-left:24px">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation">Transcriptional regulation</a>
 +
                </li>
 +
                <li class="menu-item menu-item-bullet" style="position: relative;left:200px;opacity:0;padding-left:24px">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Plasmid_Copy_Number_Control">Plasmid copy number control</a>
 +
                </li>
 +
                <li class="menu-item menu-item-bullet" style="position: relative;left:200px;opacity:0;padding-left:24px">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control">Post-translational regulation</a>
 +
                </li>
 +
                <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Demonstrate">Demonstration</a>
 +
                </li>
 +
                <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Parts">Parts</a>
 +
                </li>
 +
                <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Measurement">Measurement</a>
 +
                </li>
 +
            </ul>
 +
            <ul class="menu sub-menu-modeling sub-menu">
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                        <a href="#" class="return" onclick="returnMenu('modeling')">Back to Main Menu</a>
 +
                    </li>
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                      <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Model">Overview</a>
 +
                    </li>
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                      <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Degradation_Rate_Control_Model">Post-translational regulation</a>
 +
                    </li>
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                      <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Transcriptional_Regulation_CarH">Transcriptional regulation (CarH)</a>
 +
                    </li>
 +
 
 +
                </ul>
 +
                <ul class="menu sub-menu-software sub-menu">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                             <a href="#" class="return return-project" onclick="returnMenu('project')">Back to Main Menu</a>
+
                             <a href="#" class="return" onclick="returnMenu('software')">Back to Main Menu</a>
 
                         </li>
 
                         </li>
 +
                    </ul>
 +
            <ul class="menu sub-menu-hp sub-menu">
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                        <a href="#" class="return" onclick="returnMenu('hp')">Back to Main Menu</a>
 +
                    </li>
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0;white-space: nowrap">
 +
                        <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Human_Practices">Integrated Human Practices</a>
 +
                    </li>
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                        <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Public_Engagement">Education & Public Engagement</a>
 +
                    </li>
 +
                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
 +
                        <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Collaborations">Collaboration</a>
 +
                    </li>
 +
                </ul>
 +
                <ul class="menu sub-menu-team sub-menu">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                             <a href="#">Inspiration & Description</a>
+
                             <a href="#" class="return" onclick="returnMenu('team')">Back to Main Menu</a>
 
                         </li>
 
                         </li>
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                             <a href="#">Design & Results</a>
+
                             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Team">Members</a>
 
                         </li>
 
                         </li>
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                             <a href="#">Demonstration</a>
+
                             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Attributions">Attributions</a>
 
                         </li>
 
                         </li>
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                         <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                             <a href="#">Parts</a>
+
                             <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Sponsors">Sponsors</a>
 
                         </li>
 
                         </li>
 
                     </ul>
 
                     </ul>
−
                    <ul class="menu sub-menu-modeling sub-menu">
 
−
                            <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
−
                                <a href="#" class="return" onclick="returnMenu('modeling')">Back to Main Menu</a>
 
−
                            </li>
 
  
−
                        </ul>
+
                     <ul class="menu sub-menu-notebook sub-menu">
−
                        <ul class="menu sub-menu-software sub-menu">
+
−
                                <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
−
                                    <a href="#" class="return" onclick="returnMenu('software')">Back to Main Menu</a>
+
−
                                </li>
+
−
                            </ul>
+
−
                     <ul class="menu sub-menu-hp sub-menu">
+
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                                 <a href="#" class="return" onclick="returnMenu('hp')">Back to Main Menu</a>
+
                                 <a href="#" class="return" onclick="returnMenu('notebook')">Back to Main Menu</a>
 
                             </li>
 
                             </li>
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                                 <a href="#">HP</a>
+
                                 <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Notebook">Lab journal</a>
 
                             </li>
 
                             </li>
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                                 <a href="#">HP model</a>
+
                                 <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Protocols">Protocols</a>
 
                             </li>
 
                             </li>
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
 
                             <li class="menu-item" style="position: relative;left:200px;opacity:0">
−
                                 <a href="#">Integrated</a>
+
                                 <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Safety">Safety</a>
−
                            </li>
+
−
                            <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
−
                                <a href="#">Collaboration</a>
+
 
                             </li>
 
                             </li>
 
                         </ul>
 
                         </ul>
−
                        <ul class="menu sub-menu-team sub-menu">
+
    <ul class="menu menu-main">
−
                                <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
        <li class="menu-item" style="position: relative">
−
                                    <a href="#" class="return" onclick="returnMenu('team')">Back to Main Menu</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania">Home</a>
−
                                </li>
+
        </li>
−
                                <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
        <li class="menu-item menu-item-sub" onclick="expandSubMenu('project')" style="position: relative">
−
                                    <a href="#">Members</a>
+
            <a href="#">Project</a>
−
                                </li>
+
        </li>
−
                                <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
        <li class="menu-item menu-item-sub" onclick="expandSubMenu('modeling')" style="position: relative">
−
                                    <a href="#">Attributions</a>
+
            <a href="#">Modeling</a>
−
                                </li>
+
        </li>
−
                                <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
        <li class="menu-item" style="position: relative">
−
                                    <a href="#">Sponsors</a>
+
            <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Software">Software</a>
−
                                </li>
+
        </li>
−
                            </ul>
+
        <li class="menu-item menu-item-sub" onclick="expandSubMenu('hp')" style="position: relative">
−
 
+
            <a href="#">Human Practices</a>
−
                            <ul class="menu sub-menu-notebook sub-menu">
+
        </li>
−
                                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
        <li class="menu-item menu-item-sub" onclick="expandSubMenu('team')" style="position: relative">
−
                                        <a href="#" class="return" onclick="returnMenu('notebook')">Back to Main Menu</a>
+
            <a href="#">Team</a>
−
                                    </li>
+
        </li>
−
                                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
        <li class="menu-item menu-item-sub" onclick="expandSubMenu('notebook')" style="position: relative">
−
                                        <a href="#">Lab journal</a>
+
            <a href="#">Notebook</a>
−
                                    </li>
+
        </li>
−
                                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
    </ul>
−
                                        <a href="#">Protocols</a>
+
</div>
−
                                    </li>
+
</div>
−
                                    <li class="menu-item" style="position: relative;left:200px;opacity:0">
+
         <div class="row no-gutter" style="margin:0">
−
                                        <a href="#">Safety</a>
+
     <video width="100%" autoplay muted style="z-index:10;object-fit:cover;height:700px" class="logo-video">
−
                                    </li>
+
−
                                </ul>
+
−
            <ul class="menu menu-main">
+
−
                <li class="menu-item" style="position: relative">
+
−
                    <a href="#">Home</a>
+
−
                </li>
+
−
                <li class="menu-item menu-item-sub" onclick="expandSubMenu('project')" style="position: relative">
+
−
                    <a href="#">Project</a>
+
−
                </li>
+
−
                <li class="menu-item menu-item-sub" onclick="expandSubMenu('modeling')" style="position: relative">
+
−
                    <a href="#">Modeling</a>
+
−
                </li>
+
−
                <li class="menu-item menu-item-sub" onclick="expandSubMenu('software')" style="position: relative">
+
−
                    <a href="#">Software</a>
+
−
                </li>
+
−
                <li class="menu-item menu-item-sub" onclick="expandSubMenu('hp')" style="position: relative">
+
−
                    <a href="#">Human Practices</a>
+
−
                </li>
+
−
                <li class="menu-item menu-item-sub" onclick="expandSubMenu('team')" style="position: relative">
+
−
                    <a href="#">Team</a>
+
−
                </li>
+
−
                <li class="menu-item menu-item-sub" onclick="expandSubMenu('notebook')" style="position: relative">
+
−
                    <a href="#">Notebook</a>
+
−
                </li>
+
−
            </ul>
+
−
        </div>
+
−
    </div>
+
−
         <div class="row no-gutters">
+
−
     <video width="100%" autoplay muted style="z-index:10">
+
 
         <source src="https://2019.igem.org/wiki/images/0/0b/T--Vilnius-Lithuania--final.mp4" type="video/mp4">
 
         <source src="https://2019.igem.org/wiki/images/0/0b/T--Vilnius-Lithuania--final.mp4" type="video/mp4">
 
       </video>
 
       </video>
−
        </div>
+
</div>
  
 
<div class="container">
 
<div class="container">
Line 643: Line 662:
  
 
         <div class="row no-gutters">
 
         <div class="row no-gutters">
−
             <div class="col-2 d-none d-sm-none d-md-block scroll-fade"></div>
+
             <div class="col-2 d-none d-sm-none d-md-block"></div>
−
             <div class="col-md-10 col-xs-12 main-container">
+
             <div class="col-md-10 col-xs-12 main-container scroll-fade">
 
                 <img src="https://2019.igem.org/wiki/images/b/b9/T--Vilnius-Lithuania--vizualas1.png" class="image-hover">
 
                 <img src="https://2019.igem.org/wiki/images/b/b9/T--Vilnius-Lithuania--vizualas1.png" class="image-hover">
 
                 <div class="text">
 
                 <div class="text">
 
                     <h1>Project Description</h1>
 
                     <h1>Project Description</h1>
−
                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec non vulputate nunc, id dignissim lacus. Proin sodales nunc sit amet eros suscipit, nec condimentum turpis dapibus. Nullam vitae tortor ut augue tempus tempus commodo ut enim. Integer faucibus non enim eu hendrerit. Praesent sed lacinia ante, at imperdiet eros. Morbi elementum dolor nibh, ac aliquet odio dictum at. Fusce urna sapien, commodo non convallis et, interdum sit amet lorem. In tincidunt bibendum nisl a placerat. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos.  </p>                
+
                     <p>Optogenetic tools offer unprecedented ways to control cellular behavior in a precise and spatiotemporal manner. The integration of light-regulated systems into synthetic biology would drastically increase the level of control and open up a way for creative and innovative applications. However, current light modular systems have a variety of serious drawbacks, such as low dynamic range, slow response time, lack of portability, and a lack of parts. What is more, it is possible to modulate bacteria dynamics only at the transcriptional level. To address these shortcomings and open new avenues in bacteria regulation, we decided to develop our project “Colight” - a multi-level collection of optogenetic tools for modular bacteria control. Our newly developed systems fulfill the gaps in optogenetic bacteria control in transcription regulation and open up a possibility of light control expression dynamics in plasmid copy number and post-translational levels.</p>  
 +
<br>
 +
<a href="https://2019.igem.org/Team:Vilnius-Lithuania/Description">READ MORE</a>               
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
Line 660: Line 681:
 
                     <h1>Software.<br>
 
                     <h1>Software.<br>
 
                     Metagenomic mining</h1>
 
                     Metagenomic mining</h1>
−
                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec non vulputate nunc, id dignissim lacus. Proin sodales nunc sit amet eros suscipit, nec condimentum turpis dapibus. Nullam vitae tortor ut augue tempus tempus commodo ut enim. Integer faucibus non enim eu hendrerit. Praesent sed lacinia ante, at imperdiet eros. Morbi elementum dolor nibh, ac aliquet odio dictum at. Fusce urna sapien, commodo non convallis et, interdum sit amet lorem. In tincidunt bibendum nisl a placerat. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. </p>                
+
                     <p>While searching for proteins with light-inducible domains, we managed to build software that enables the user to quickly and effortlessly discover proteins of interest by scanning through terabytes of metagenomic sequences. Our software takes advantage of MG-RAST database API and allows users to search & download hundreds of thousands of metagenomic samples to our server with just one click in their web-browser. Then it automatically standardizes and cleans the selected sequences to prepare them for further analysis. The user can choose from a variety of pre-made protein profiles obtained from the Pfam database; the primary tool used for similarity search is HMMER. This way, our software eliminates the previously required workload & knowledge and opens up the true potential of metagenomic research.</p>      <br>
 +
<a href="https://2019.igem.org/Team:Vilnius-Lithuania/Software">READ MORE</a>          
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
Line 672: Line 694:
 
                 <img src="https://2019.igem.org/wiki/images/4/4a/T--Vilnius-Lithuania--vizualas3.png" class="image-hover">
 
                 <img src="https://2019.igem.org/wiki/images/4/4a/T--Vilnius-Lithuania--vizualas3.png" class="image-hover">
 
                 <div class="text">
 
                 <div class="text">
−
                     <h1>Modelling</h1>
+
                     <h1>Modeling</h1>
−
                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec non vulputate nunc, id dignissim lacus. Proin sodales nunc sit amet eros suscipit, nec condimentum turpis dapibus. Nullam vitae tortor ut augue tempus tempus commodo ut enim. Integer faucibus non enim eu hendrerit. Praesent sed lacinia ante, at imperdiet eros. Morbi elementum dolor nibh, ac aliquet odio dictum at. Fusce urna sapien, commodo non convallis et, interdum sit amet lorem. In tincidunt bibendum nisl a placerat. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. </p>                
+
                     <p>For the past decade, the interest to adapt control theory tools for the use in synthetic biology has been growing. While control theory is seeping its way into synthetic biology, it has not yet been explored in iGEM. Since control plays a central role in our project, Colight, we have decided to bring this sort of mathematical analysis. The models analyze the dynamics of control systems with light as an input. We check if it is possible to create potential in-silico feedback controllers. A design for an in-silico adaptive feedback controller for post-translational protein control has been constructed and tested by simulation. Most emphasis is put on the post-translational control model in which we describe our modeling approach in detail. For brevity, the same considerations are not repeated in the transcriptional regulation, and more emphasis is given to the results.</p> <br>
 +
<a href="https://2019.igem.org/Team:Vilnius-Lithuania/Model">READ MORE</a>                
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
Line 685: Line 708:
 
                     <h1>Public engagement<br>
 
                     <h1>Public engagement<br>
 
                     AND human practices</h1>
 
                     AND human practices</h1>
−
                     <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec non vulputate nunc, id dignissim lacus. Proin sodales nunc sit amet eros suscipit, nec condimentum turpis dapibus. Nullam vitae tortor ut augue tempus tempus commodo ut enim. Integer faucibus non enim eu hendrerit. Praesent sed lacinia ante, at imperdiet eros. Morbi elementum dolor nibh, ac aliquet odio dictum at. Fusce urna sapien, commodo non convallis et, interdum sit amet lorem. In tincidunt bibendum nisl a placerat. Class aptent taciti sociosqu ad litora torquent per conubia nostra, per inceptos himenaeos. </p>                
+
                     <p>The beginning of 2019 was marked by a WHO statement exposing a health crisis caused by the movement of anti-vaccination. In February we witnessed the pinnacle of this movement in Lithuania, where it manifested through the outbreak of measles. It came to light that many people are having doubts about the validity of scientific findings, and, therefore, turn to other sources for information, for example, famous people in the media, who have little to no scientific knowledge. People do not take science seriously and these views are both manifested and fueled through the media. Naturally, we raised a question – what is the cause of the miscommunication and disagreement? What is the main culprit of science skepticism? </p>  
 +
                    <br>
 +
                    <a href="https://2019.igem.org/Team:Vilnius-Lithuania/Public_Engagement">READ MORE</a>
 
                 </div>
 
                 </div>
 
             </div>
 
             </div>
Line 699: Line 724:
 
     <a href="#" id="close-menu" >&#10005;</a>
 
     <a href="#" id="close-menu" >&#10005;</a>
 
     <div id="overlay"></div>
 
     <div id="overlay"></div>
−
    <div class="laser" style="top:15%;"></div>
 
−
    <div class="laser" style="top:30%;animation-delay:360ms"></div>
 
−
    <div class="laser" style="top:45%;animation-delay:720ms"></div>
 
−
    <div class="laser" style="top:60%;animation-delay:1080ms"></div>
 
−
    <div class="laser" style="top:75%;animation-delay:1460ms"></div>
 
 
         <video autoplay muted loop id="Video">
 
         <video autoplay muted loop id="Video">
 
                 <source src="https://2019.igem.org/wiki/images/4/4a/T--Vilnius-Lithuania--blue.mp4" type="video/mp4">
 
                 <source src="https://2019.igem.org/wiki/images/4/4a/T--Vilnius-Lithuania--blue.mp4" type="video/mp4">

Latest revision as of 02:40, 22 October 2019

Project Description

Optogenetic tools offer unprecedented ways to control cellular behavior in a precise and spatiotemporal manner. The integration of light-regulated systems into synthetic biology would drastically increase the level of control and open up a way for creative and innovative applications. However, current light modular systems have a variety of serious drawbacks, such as low dynamic range, slow response time, lack of portability, and a lack of parts. What is more, it is possible to modulate bacteria dynamics only at the transcriptional level. To address these shortcomings and open new avenues in bacteria regulation, we decided to develop our project “Colight” - a multi-level collection of optogenetic tools for modular bacteria control. Our newly developed systems fulfill the gaps in optogenetic bacteria control in transcription regulation and open up a possibility of light control expression dynamics in plasmid copy number and post-translational levels.


READ MORE


Software.
Metagenomic mining

While searching for proteins with light-inducible domains, we managed to build software that enables the user to quickly and effortlessly discover proteins of interest by scanning through terabytes of metagenomic sequences. Our software takes advantage of MG-RAST database API and allows users to search & download hundreds of thousands of metagenomic samples to our server with just one click in their web-browser. Then it automatically standardizes and cleans the selected sequences to prepare them for further analysis. The user can choose from a variety of pre-made protein profiles obtained from the Pfam database; the primary tool used for similarity search is HMMER. This way, our software eliminates the previously required workload & knowledge and opens up the true potential of metagenomic research.


READ MORE


Modeling

For the past decade, the interest to adapt control theory tools for the use in synthetic biology has been growing. While control theory is seeping its way into synthetic biology, it has not yet been explored in iGEM. Since control plays a central role in our project, Colight, we have decided to bring this sort of mathematical analysis. The models analyze the dynamics of control systems with light as an input. We check if it is possible to create potential in-silico feedback controllers. A design for an in-silico adaptive feedback controller for post-translational protein control has been constructed and tested by simulation. Most emphasis is put on the post-translational control model in which we describe our modeling approach in detail. For brevity, the same considerations are not repeated in the transcriptional regulation, and more emphasis is given to the results.


READ MORE


Public engagement
AND human practices

The beginning of 2019 was marked by a WHO statement exposing a health crisis caused by the movement of anti-vaccination. In February we witnessed the pinnacle of this movement in Lithuania, where it manifested through the outbreak of measles. It came to light that many people are having doubts about the validity of scientific findings, and, therefore, turn to other sources for information, for example, famous people in the media, who have little to no scientific knowledge. People do not take science seriously and these views are both manifested and fueled through the media. Naturally, we raised a question – what is the cause of the miscommunication and disagreement? What is the main culprit of science skepticism?


READ MORE

↑ ☰ ✕