|
|
| Line 21: |
Line 21: |
| | height: 100%; | | height: 100%; |
| | z-index: 9999; | | z-index: 9999; |
| − | background: #2B2B35; | + | background: #292935; |
| | } | | } |
| | | | |
| Line 59: |
Line 59: |
| | <body> | | <body> |
| | <div class="se-pre-con"> | | <div class="se-pre-con"> |
| − | <img src="https://2019.igem.org/wiki/images/8/83/T--Tuebingen--preloader.gif"> | + | <img src="https://2019.igem.org/wiki/images/4/49/T--Tuebingen--DNA_gif.gif"> |
| | </div> | | </div> |
| | <script src="https://2019.igem.org/Team:Tuebingen/jqueryLoadJS?action=raw&ctype=text/javascript"></script> | | <script src="https://2019.igem.org/Team:Tuebingen/jqueryLoadJS?action=raw&ctype=text/javascript"></script> |
| Line 1,721: |
Line 1,721: |
| | max-width: 100%; | | max-width: 100%; |
| | margin-bottom: 1rem; | | margin-bottom: 1rem; |
| − | background-color: transparent | + | background-color: transparent; |
| | + | z-index: 100; |
| | } | | } |
| | .veggie td, | | .veggie td, |
| Line 1,730: |
Line 1,731: |
| | color: #F4F0DE; | | color: #F4F0DE; |
| | border-bottom: 1px solid #F4F0DE; | | border-bottom: 1px solid #F4F0DE; |
| | + | z-index:100; |
| | } | | } |
| | .veggie th { | | .veggie th { |
| − | background-color: #2ecc71; | + | background-color: #2ecc71 !important; |
| − | color: #232323 !important | + | color: #232323 !important; |
| | + | z-index:100 |
| | } | | } |
| | .veggie caption { | | .veggie caption { |
| | color: #adb3b7; | | color: #adb3b7; |
| | + | z-index:100; |
| | } | | } |
| | .veggie thead th { | | .veggie thead th { |
| | vertical-align: bottom; | | vertical-align: bottom; |
| − | border-bottom: 2px solid #dee2e6 | + | border-bottom: 2px solid #dee2e6; |
| | + | z-index:100; |
| | } | | } |
| | .veggie tbody+tbody { | | .veggie tbody+tbody { |
| − | border-top: 2px solid #dee2e6 | + | border-top: 2px solid #dee2e6; |
| | + | z-index:100; |
| | } | | } |
| | | | |
| Line 1,749: |
Line 1,755: |
| | .veggie { | | .veggie { |
| | font-size: xx-small; | | font-size: xx-small; |
| | + | z-index:100; |
| | } | | } |
| | } | | } |
| Line 9,763: |
Line 9,770: |
| | </style> | | </style> |
| | <head> | | <head> |
| − | <title>GLP.exe</title> | + | <title>GLP.exe Characterized Part</title> |
| | <meta charset="utf-8"> | | <meta charset="utf-8"> |
| | <meta name="viewport" content="width=device-width, initial-scale=1"> | | <meta name="viewport" content="width=device-width, initial-scale=1"> |
| Line 10,255: |
Line 10,262: |
| | </head> | | </head> |
| | | | |
| − | <body style=""> | + | <body style=""> |
| − | <section id="nav-placeholder">
| + | |
| − | <nav class="shadow navbar navbar-expand-md navbar-dark fixed-top"
| + | |
| − | style="background-color: rgba(30, 30, 30, 0.3); z-index: 100;">
| + | |
| − | <a class="navbar-brand" href="https://2019.igem.org/Team:Tuebingen">
| + | |
| − | <img src="https://2019.igem.org/wiki/images/2/2a/T--Tuebingen--white_logo_animated.gif" alt="logo"
| + | |
| − | style="width:70px;">
| + | |
| − | <h5 class="d-inline ml-3" style="font-family: 'Righteous', cursive; font-weight: normal;">GLP.exe</h5>
| + | |
| − | </a>
| + | |
| − | <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#collapsibleNavbar">
| + | |
| − | <span class="navbar-toggler-icon"></span>
| + | |
| − | </button>
| + | |
| − | <div class="collapse navbar-collapse justify-content-around" id="collapsibleNavbar">
| + | |
| − | <ul class="navbar-nav">
| + | |
| − | <li id="ourStoryButton" class="nav-item mx-3">
| + | |
| − | <a class="nav-link" href="/Team:Tuebingen/Story"
| + | |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">OUR
| + | |
| − | STORY</a>
| + | |
| − | </li>
| + | |
| | | | |
| − | <li class="nav-item dropdown mx-3"> | + | <section id="nav-placeholder"> |
| − | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" | + | <nav class="shadow navbar navbar-expand-md navbar-dark fixed-top" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">
| + | style="background-color: rgba(30, 30, 30, 0.3); z-index: 100;"> |
| − | PROJECT
| + | <a class="navbar-brand" href="https://2019.igem.org/Team:Tuebingen"> |
| − | </a>
| + | <img src="https://2019.igem.org/wiki/images/2/2a/T--Tuebingen--white_logo_animated.gif" alt="logo" |
| − | <div class="dropdown-menu"> | + | style="width:70px;"> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Description" | + | <h5 class="d-inline ml-3" style="font-family: 'Righteous', cursive; font-weight: normal;">GLP.exe</h5> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Description</a>
| + | </a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Killswitch" | + | <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#collapsibleNavbar"> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Kill Switch</a>
| + | <span class="navbar-toggler-icon"></span> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Nissle" | + | </button> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Nissle</a>
| + | <div class="collapse navbar-collapse justify-content-around" id="collapsibleNavbar"> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Incretin" | + | <ul class="navbar-nav"> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Incretin</a>
| + | <li id="ourStoryButton" class="nav-item mx-3"> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Model" | + | <a class="nav-link" href="/Team:Tuebingen/Story" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Model</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">OUR |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Software" | + | STORY</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Software</a>
| + | </li> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Results" | + | |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Results</a>
| + | <li class="nav-item dropdown mx-3"> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Demonstrate" | + | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Demonstrate</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;"> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Notebook" | + | PROJECT |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Notebook</a>
| + | </a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Outlook" | + | <div class="dropdown-menu"> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Outlook</a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Description" |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Entrepreneurship" | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Description</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Entrepreneurship</a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Killswitch" |
| − | </div>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Kill Switch</a> |
| − | </li>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Nissle" |
| − | <li class="nav-item dropdown mx-3">
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Nissle</a> |
| − | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" | + | <a class="dropdown-item" href="/Team:Tuebingen/Incretin" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Incretin</a> |
| − | PARTS
| + | <a class="dropdown-item" href="/Team:Tuebingen/Model" |
| − | </a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Model</a> |
| − | <div class="dropdown-menu"> | + | <a class="dropdown-item" href="/Team:Tuebingen/Software" |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Parts" | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Software</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Parts
| + | <a class="dropdown-item" href="/Team:Tuebingen/Results" |
| − | Overview</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Results</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Basic_Part" | + | <a class="dropdown-item" href="/Team:Tuebingen/Demonstrate" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Basic
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Demonstrate</a> |
| − | Parts</a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Notebook" |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Improved_Part" | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Notebook</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Improved
| + | <a class="dropdown-item" href="/Team:Tuebingen/Outlook" |
| − | Parts</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Outlook</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Characterized_Part" | + | <a class="dropdown-item" href="/Team:Tuebingen/Entrepreneurship" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Improved
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Entrepreneurship</a> |
| − | Parts</a>
| + | </div> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Composite_Part" | + | </li> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Composite
| + | <li class="nav-item dropdown mx-3"> |
| − | Parts</a>
| + | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Part_Collection" | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;"> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Part
| + | PARTS |
| − | Collection</a>
| + | </a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Downloads" | + | <div class="dropdown-menu"> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Downloads</a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Parts" |
| − | </div>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Parts |
| − | </li>
| + | Overview</a> |
| − | | + | <a class="dropdown-item" href="/Team:Tuebingen/Basic_Part" |
| − | <li class="nav-item dropdown mx-3">
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Basic |
| − | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" | + | Parts</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal;color: #2ecc71;">
| + | <a class="dropdown-item" href="/Team:Tuebingen/Improved_Part" |
| − | PEOPLE
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Improved |
| − | </a>
| + | Parts</a> |
| − | <div class="dropdown-menu"> | + | <a class="dropdown-item" href="/Team:Tuebingen/Characterized_Part" |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Team" | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Characterized |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Team</a>
| + | Parts</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Collaborations" | + | <a class="dropdown-item" href="/Team:Tuebingen/Composite_Part" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Collaborations</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Composite |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Sponsors" | + | Parts</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Sponsors</a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Part_Collection" |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Attributions" | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Part |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Attributions</a>
| + | Collection</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Gallery" | + | <a class="dropdown-item" href="/Team:Tuebingen/Downloads" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Gallery</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Downloads</a> |
| − | </div>
| + | </div> |
| − | </li>
| + | </li> |
| − | | + | |
| − | <li class="nav-item dropdown mx-3">
| + | <li class="nav-item dropdown mx-3"> |
| − | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" | + | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">
| + | style="font-family: 'Righteous', cursive; font-weight: normal;color: #2ecc71;"> |
| − | HUMAN PRACTICES
| + | PEOPLE |
| − | </a>
| + | </a> |
| − | <div class="dropdown-menu"> | + | <div class="dropdown-menu"> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Human_Practices" | + | <a class="dropdown-item" href="/Team:Tuebingen/Team" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Overview</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Team</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Public_Engagement" | + | <a class="dropdown-item" href="/Team:Tuebingen/Collaborations" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Education & Public Engagement</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Collaborations</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Experts" | + | <a class="dropdown-item" href="/Team:Tuebingen/Sponsors" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Experts</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Sponsors</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/SDG" | + | <a class="dropdown-item" href="/Team:Tuebingen/Attributions" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Sustainable Development Goals</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Attributions</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Experimenta" | + | <a class="dropdown-item" href="/Team:Tuebingen/Gallery" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Experimenta
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Gallery</a> |
| − | Science Center</a>
| + | </div> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Survey" | + | </li> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Survey</a>
| + | |
| − | </div>
| + | <li class="nav-item dropdown mx-3"> |
| − | </li>
| + | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" |
| − | <li class="nav-item dropdown mx-3">
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;"> |
| − | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" | + | HUMAN PRACTICES |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">
| + | </a> |
| − | AWARDS
| + | <div class="dropdown-menu"> |
| − | </a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Human_Practices" |
| − | <div class="dropdown-menu"> | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Overview</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Human_Practices" | + | <a class="dropdown-item" href="/Team:Tuebingen/Public_Engagement" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Integrated Human Practise</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Education & Public Engagement</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Public_Engagement" | + | <a class="dropdown-item" href="/Team:Tuebingen/Experts" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Education & Public Engagement</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Experts</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Entrepreneurship" | + | <a class="dropdown-item" href="/Team:Tuebingen/SDG" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Supporting Entrepreneurship</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Sustainable Development Goals</a> |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Model" | + | <a class="dropdown-item" href="/Team:Tuebingen/Experimenta" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Model</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Experimenta |
| − | <a class="dropdown-item" href="/Team:Tuebingen/Software" | + | Science Center</a> |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Software</a>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Survey" |
| − | </div>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Survey</a> |
| − | </li>
| + | </div> |
| − | | + | </li> |
| − | <li class="nav-item mx-3">
| + | <li class="nav-item dropdown mx-3"> |
| − | <a class="nav-link" href="/Team:Tuebingen/Safety" | + | <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">SAFETY</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;"> |
| − | </li>
| + | AWARDS |
| − | | + | </a> |
| − | <li class="nav-item mx-3">
| + | <div class="dropdown-menu"> |
| − | <a class="nav-link" href="https://igem.org/2019_Judging_Form?team=Tuebingen" target="_blank" | + | <a class="dropdown-item" href="/Team:Tuebingen/Human_Practices" |
| − | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">JUDGING FORM ⇗</a>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Integrated Human Practise</a> |
| − | </li>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Public_Engagement" |
| − | | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Education & Public Engagement</a> |
| − | <li class="nav-item mx-1 pt-2">
| + | <a class="dropdown-item" href="/Team:Tuebingen/Entrepreneurship" |
| − | <a href="https://www.facebook.com/igemtuebingen/" class="fa fa-facebook" target="_blank"></a> | + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Supporting Entrepreneurship</a> |
| − | </li>
| + | <a class="dropdown-item" href="/Team:Tuebingen/Model" |
| − | <li class="nav-item mx-1 pt-2">
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Model</a> |
| − | <a href="https://twitter.com/iGEMTuebingen" class="fa fa-twitter" target="_blank"></a> | + | <a class="dropdown-item" href="/Team:Tuebingen/Software" |
| − | </li>
| + | style="font-family: 'Righteous', cursive; font-weight: normal; color: #2ecc71;">Software</a> |
| − | <li class="nav-item mx-1 pt-2">
| + | </div> |
| − | <a href="https://www.instagram.com/igem_tuebingen/" class="fa fa-instagram" target="_blank"></a> | + | </li> |
| − | </li>
| + | |
| − | | + | <li class="nav-item mx-3"> |
| − | </ul>
| + | <a class="nav-link" href="/Team:Tuebingen/Safety" |
| − | </div>
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| | font-size: 1.9em; | | font-size: 1.9em; |
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| | @media only screen and (max-width: 400px) { | | @media only screen and (max-width: 400px) { |
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| | .header { | | .header { |
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| | <p> | | <p> |
| | We <b>characterized</b> BBa_K398108, a salt tolerance cluster, for its resistance to potassium chloride. | | We <b>characterized</b> BBa_K398108, a salt tolerance cluster, for its resistance to potassium chloride. |
| − | This was interesting to us because it is known that high obesity, a risk factor of diabetes, | + | This was interesting to us because it is known that high obesity, a risk factor of Diabetes, |
| | correlates strongly with high salt intake [1]. Adding such a salt tolerance cluster to a probiotic | | correlates strongly with high salt intake [1]. Adding such a salt tolerance cluster to a probiotic |
| | therapeutic could provide it with a selective advantage, supporting the survival of the competition | | therapeutic could provide it with a selective advantage, supporting the survival of the competition |
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| | containing 0M to 0.8M KCl. Data was gained in triplicates and growth was measured as OD600 with | | containing 0M to 0.8M KCl. Data was gained in triplicates and growth was measured as OD600 with |
| | a Nanospectrometer (Implen) every 45 minutes (in accordance with the previous characterization | | a Nanospectrometer (Implen) every 45 minutes (in accordance with the previous characterization |
| − | for NaCl)(Fig. 1). For the analysis, OD600 values were transformed to logarithmic values and | + | for NaCl) (Fig. 1). For the analysis, OD600 values were transformed to logarithmic values and |
| | subsequently nonlinearly fitted to a growth curve using GraphPad Prism8.0 (Fig.2). | | subsequently nonlinearly fitted to a growth curve using GraphPad Prism8.0 (Fig.2). |
| | </p> | | </p> |
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| | href="http://parts.igem.org/wiki/images/4/41/T--Tuebingen--whiteshortprimary_salt.png" | | href="http://parts.igem.org/wiki/images/4/41/T--Tuebingen--whiteshortprimary_salt.png" |
| | data-effect="mfp-zoom-out" | | data-effect="mfp-zoom-out" |
| − | title="Some title"> | + | title="Fig. 1: Mean of the triplicates and standard deviation of the measured growth curves"> |
| | <img src="http://parts.igem.org/wiki/images/4/41/T--Tuebingen--whiteshortprimary_salt.png" | | <img src="http://parts.igem.org/wiki/images/4/41/T--Tuebingen--whiteshortprimary_salt.png" |
| | class="figure-img img-fluid rounded" | | class="figure-img img-fluid rounded" |
| | alt="A generic square placeholder image with rounded corners in a figure."></a> | | alt="A generic square placeholder image with rounded corners in a figure."></a> |
| − | <figcaption style="font-size: small; color: #F4F0DE">Fig. 1: Mean of the triplicates and standard deviation of the measured growth curves.</figcaption> | + | <figcaption style="font-size: small; color: #F4F0DE">Figure 1: Mean of the triplicates and standard deviation of the measured growth curves.</figcaption> |
| | </figure> | | </figure> |
| | | | |
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| | href="http://parts.igem.org/wiki/images/9/99/T--Tuebingen--salt_analysis_Table.png" | | href="http://parts.igem.org/wiki/images/9/99/T--Tuebingen--salt_analysis_Table.png" |
| | data-effect="mfp-zoom-out" | | data-effect="mfp-zoom-out" |
| − | title="Some title"> | + | title="Table 1: Result of transformation to logarithmic |
| | + | scale and nonlinear fit to exponential population growth"> |
| | <img src="http://parts.igem.org/wiki/images/9/99/T--Tuebingen--salt_analysis_Table.png" | | <img src="http://parts.igem.org/wiki/images/9/99/T--Tuebingen--salt_analysis_Table.png" |
| | class="figure-img img-fluid rounded" | | class="figure-img img-fluid rounded" |
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| | href="http://parts.igem.org/wiki/images/f/fe/T--Tuebingen--salttol2.png" | | href="http://parts.igem.org/wiki/images/f/fe/T--Tuebingen--salttol2.png" |
| | data-effect="mfp-zoom-out" | | data-effect="mfp-zoom-out" |
| − | title="Some title"> | + | title="Fig 2: Growth rate [h-1] and [%] growth of salt tolerant E. Coli DH5alpha and wt DH5alpha"> |
| | <img src="http://parts.igem.org/wiki/images/f/fe/T--Tuebingen--salttol2.png" | | <img src="http://parts.igem.org/wiki/images/f/fe/T--Tuebingen--salttol2.png" |
| | class="figure-img img-fluid rounded" | | class="figure-img img-fluid rounded" |
| | alt="A generic square placeholder image with rounded corners in a figure."></a> | | alt="A generic square placeholder image with rounded corners in a figure."></a> |
| − | <figcaption style="font-size: small; color: #F4F0DE">Fig 2: Growth rate [h-1] and [%] growth of salt tolerant E.Coli DH5alpha and wt DH5alpha. | + | <figcaption style="font-size: small; color: #F4F0DE">Figure 2: Growth rate [h-1] and [%] growth of salt tolerant <i>E. coli</i> DH5alpha and wt DH5alpha. |
| | Growth rate was calculated by using the proposed doubling time of the fit. [%] growth was calculated | | Growth rate was calculated by using the proposed doubling time of the fit. [%] growth was calculated |
| | by the normalisation of the growth rate of the salt tolerant DH5alpha against the wild type DH5alpha. | | by the normalisation of the growth rate of the salt tolerant DH5alpha against the wild type DH5alpha. |
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