Team:Stuttgart/Contribution

Project

Contribution

We added quantitative experimental characterization to the following biobricks:

• BBa_J23100
• BBa_J23112
• BBa_J23118

These parts are a family of constitutive promoters. They were present in plasmid BBa_J61002). We analyzed the influence of the mentioned promoters on the expression of RFP, both on protein level (fluorescence spectroscopy) and mRNA level (RT-qPCR).

Materials and Methods

Forward and reverse primers were designed for RFP mRNA amplification:

• forward primer
• • forward primer

: atggcttcctccgaagacg

reverse primer: actcaccgtcttgcaggg

The expected amplification product has a length of 352 bp.