Difference between revisions of "Team:Stuttgart/Contribution"

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                Project
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          Project
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            <h1 class="title">
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        <h1 class="title">
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                Contribution
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          Contribution
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            We added quantitative experimental characterization to the following biobricks:
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        We added quantitative experimental characterization to the following biobricks:
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            <ul>
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        <ul>
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                <li><a href="http://parts.igem.org/Part:BBa_J23100">Part:BBa_J23100</a></li>
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          <li><a href="http://parts.igem.org/Part:BBa_J23100">BBa_J23100</a></li>
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                <li><a href="http://parts.igem.org/Part:BBa_J23112">BBa_J23112</a></li>
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          <li><a href="http://parts.igem.org/Part:BBa_J23112">BBa_J23112</a></li>
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                <li><a href="http://parts.igem.org/Part:BBa_J23118">BBa_J23118</a></li>
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          <li><a href="http://parts.igem.org/Part:BBa_J23118">BBa_J23118</a></li>
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            These parts are a family of constitutive
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        These parts are a family of constitutive promoters. They were present in plasmid
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            promoters. They were present in plasmid <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a>). We
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        <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a>). We analyzed the influence of the mentioned
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            analyzed the influence of the mentioned promoters on the expression of RFP, both on protein level
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        promoters on the expression of RFP, both on protein level (fluorescence spectroscopy) and mRNA level (RT-qPCR).
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            (fluorescence spectroscopy) and mRNA level (RT-qPCR).
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            <p><strong>Materials and Methods</strong></p>
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            <p>Forward and reverse primers were designed for RFP mRNA amplification:</p>
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              <h3 class="title is-2">Materials and Methods</h3>
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            <p>forward primer:
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              <p>Forward and reverse primers were designed for RFP mRNA amplification:</p>
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            <code>atggcttcctccgaagacg</code>
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              <ul>
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            </p>
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                  <li>forward primer</li>
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            <p>reverse primer: actcaccgtcttgcaggg</p>
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                  <li><ul>
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            <p>The expected amplification product has a length of 352 bp.</p>
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                      <li>forward primer</li>
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                  </ul></li>
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              <p>:
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                  <code>atggcttcctccgaagacg</code>
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              <p>reverse primer: actcaccgtcttgcaggg</p>
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              <p>The expected amplification product has a length of 352 bp.</p>
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Revision as of 21:46, 20 October 2019

Project

Contribution

We added quantitative experimental characterization to the following biobricks: These parts are a family of constitutive promoters. They were present in plasmid BBa_J61002). We analyzed the influence of the mentioned promoters on the expression of RFP, both on protein level (fluorescence spectroscopy) and mRNA level (RT-qPCR).

Materials and Methods

Forward and reverse primers were designed for RFP mRNA amplification:

  • forward primer
    • forward primer

: atggcttcctccgaagacg

reverse primer: actcaccgtcttgcaggg

The expected amplification product has a length of 352 bp.