Difference between revisions of "Team:Stuttgart/Contribution"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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          Project
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            Contribution
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<h1>Characterization or Contribution </h1>
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Bronze Medal Criterion #5
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<b>Characterization - Standard Tracks:</b> Convince the judges that you have added quantitative experimental characterization data to an existing Part from the Registry of Standard Biological Parts. Clearly document the experimental characterization on the Part's Main Page on the Registry (see the Registry <a href="http://parts.igem.org/Help:Document_Parts">Document Parts page</a> for instructions). The part that you are characterizing must NOT be from a 2019 part number range. It is acceptable to add new data to an already highly characterized part. Please see the <a href="https://2019.igem.org/Measurement/Resources">Measurement Resources page</a> for more information about experimental characterization data. Sample submission is not required. 
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You should list the part(s) you characterized for this medal criterion on this page and include links to the part's Registry pages, but <b>all data must be added to the Part's Main Page on the Registry</b>.
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          We added quantitative experimental characterization to the following biobricks:
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          <a href="http://parts.igem.org/Part:BBa_J23100">Part:BBa_J23100</a>,
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          <a href="http://parts.igem.org/Part:BBa_J23112">BBa_J23112</a> and
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<b>Contribution - Special Tracks:</b> Document on your team wiki at least one new substantial contribution to the iGEM community that showcases a project related to BioBricks. This contribution should be central to your project and equivalent in difficulty to characterizing a BioBrick Part.
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          <a href="http://parts.igem.org/Part:BBa_J23118">BBa_J23118</a>. These parts are a family of constitutive
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          promoters. They were present in plasmid <a href="http://parts.igem.org/Part:BBa_J61002">BBa_J61002</a>). We
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          analyzed the influence of the mentioned promoters on the expression of RFP, both on protein level
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          (fluorescence spectroscopy) and mRNA level (RT-qPCR).
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              <p><strong>Materials and Methods</strong></p>
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              <p>Forward and reverse primers were designed for RFP mRNA amplification:</p>
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              <p>forward primer: <pre>atggcttcctccgaagacg</pre></p>
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              <p>reverse primer: actcaccgtcttgcaggg</p>
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              <p>The expected amplification product has a length of 352 bp.</p>
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Revision as of 21:41, 20 October 2019

Project

Contribution

We added quantitative experimental characterization to the following biobricks: Part:BBa_J23100, BBa_J23112 and BBa_J23118. These parts are a family of constitutive promoters. They were present in plasmid BBa_J61002). We analyzed the influence of the mentioned promoters on the expression of RFP, both on protein level (fluorescence spectroscopy) and mRNA level (RT-qPCR).

Materials and Methods

Forward and reverse primers were designed for RFP mRNA amplification:

forward primer: 

atggcttcctccgaagacg

reverse primer: actcaccgtcttgcaggg

The expected amplification product has a length of 352 bp.