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| − | <script>
| + | <img src="https://2019.igem.org/wiki/images/3/34/T--Stuttgart--DNA%2BAlgen.png"/> |
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| − | .vibrio-bg-img {
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| − | background: url("https://2019.igem.org/wiki/images/9/9e/T--Stuttgart--index-poster-right-1.png");
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| − | background-repeat: no-repeat;
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| − | background-size: contain;
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| − | .vibrio-bg-img-2 {
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| − | </style>
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| − | <section class="hero is-light">
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| − | <div class="hero-body">
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| − | <div class="container">
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| − | <h1 class="title">
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| − | <img
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| − | width="40%"
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| − | src="https://2019.igem.org/wiki/images/thumb/3/30/T--Stuttgart--poster-logo.png/800px-T--Stuttgart--poster-logo.png"
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| − | />
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| − | </h1>
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| − | <h1 class="subtitle">
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| − | <span class="text-green">Phycotrophic</span> Codon-optimized
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| − | <span class="text-blue">Vibrio natriegens</span>
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| − | </h1>
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| − | </div>
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| − | </div>
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| − | </section>
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| − |
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| − | <div class="container">
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| − | <div class="tile">
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| − | <div class="tile is-vertical">
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| − | <div class="tile is-child">
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| − | <img
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| − | src="https://2019.igem.org/wiki/images/1/15/T--Stuttgart--index-poster-left-1.png"
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| − | />
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| − | </div>
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| − | <div class="tile is-child">
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| − | <div
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| − | style="
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| − | border-bottom: 3px solid #008416;"
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| − | class="title"
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| − | >
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| − | <h3>Idea</h3>
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| − | </div>
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| − | <ul>
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| − | <li>
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| − | For bioprocesses, glucose is commonly used as a substrate, derived
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| − | from sugarcane
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| − | </li>
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| − | <li>
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| − | <strong>Drawback</strong>: Cultivation is related to the waste of
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| − | drinking water and arable land in less developed countries
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| − | </li>
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| − | <li>
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| − | Use a fast-growing
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| − | <span class="text-green">microalgae</span> like
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| − | <span class="text-green"><i>Chlorella vulgaris</i></span> as a CO2
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| − | fixing,
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| − | <span class="text-green">climate friendly substrate</span> for the
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| − | cultivation of
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| − | <i>Vibrio natriegens</i>
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| − | </li>
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| − | <li>
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| − | <strong>Aim</strong>: Make biological cultivations more
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| − | <span class="text-green">eco-friendly</span>, sustainable and
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| − | future-oriented in a climate-changing world
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| − | </li>
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| − | </ul>
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| − | </div>
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| − | <div class="tile hideme is-child">
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| − | <img
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| − | src="https://2019.igem.org/wiki/images/5/51/T--Stuttgart--index-poster-left-2.png"
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| − | />
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| − | </div>
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| − | <div class="tile hideme is-child">
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| − | <div
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| − | style="
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| − | border-bottom: 3px solid #008416;"
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| − | class="title"
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| − | >
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| − | <h3>Realization</h3>
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| − | </div>
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| − | <strong>Microalgae Compositional Analysis: </strong>
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| − | <ul>
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| − | <li>
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| − | Development of an improved downstream process for <i>Chlorella
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| − | vulgaris</i>
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| − | </li>
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| − | <li>Determination of the major biochemical composition</li>
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| − | <li>
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| − | Measurement of total carbohydrates, lipids, protein, chlorophyll
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| − | and
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| − | </li>
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| − | <li>carotenoids</li>
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| − | <li>
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| − | Development of an
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| − | <span class="text-green">algae-based culture medium</span> for
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| − | microorganisms like <i>Vibrio natriegens</i>
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| − | </li>
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| − | <li>
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| − | <strong>Outlook</strong>: Co-Cultivation of
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| − | <i>Vibrio natriegens</i> and a microalgae
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| − | </li>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div class="tile is-child">
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| − | <img
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| − | src="https://2019.igem.org/wiki/images/thumb/e/e4/T--Stuttgart--index-poster-left-graph.png/800px-T--Stuttgart--index-poster-left-graph.png"
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| − | />
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| − | </div>
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| − | </div>
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| − | <div
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| − | style="padding: 1rem;"
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| − | class="tile is-2 is-vertical is-hidden-mobile"
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| − | >
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| − | <div class="tile is-child"></div>
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| − | <div style="padding: 1rem;" class="tile is-child">
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| − | <img
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| − | class="hideme"
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| − | src="https://2019.igem.org/wiki/images/d/d5/T--Stuttgart--index-poster-center-1.png"
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| − | />
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| − | </div>
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| − | <div style="padding: 1rem;" class="tile is-child">
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| − | <img
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| − | class="hideme"
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| − | src="https://2019.igem.org/wiki/images/2/21/T--Stuttgart--index-poster-center-2.png"
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| − | />
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| − | </div>
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| − | <div style="padding: 1rem;" class="tile is-child">
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| − | <img
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| − | class="hideme"
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| − | src="https://2019.igem.org/wiki/images/9/9f/T--Stuttgart--index-poster-center-3.png"
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| − | />
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| − | </div>
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| − | <div style="padding: 1rem;" class="tile is-child">
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| − | <img
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| − | class="hideme"
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| − | src="https://2019.igem.org/wiki/images/thumb/7/78/T--Stuttgart--index-poster-center-4.png/427px-T--Stuttgart--index-poster-center-4.png"
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| − | />
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| − | </div>
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| − | <div style="padding: 1rem;" class="tile is-child"></div>
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| − | </div>
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| − | <div class="tile is-vertical">
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| − | <div class="tile is-child vibrio-bg-img">
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| − | <div
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| − | style="padding-top: 13rem; border-bottom: 3px solid #006cbf;"
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| − | class="title"
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| − | >
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| − | <h3>Idea</h3>
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| − | </div>
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| − | <ul>
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| − | <li>
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| − | <span class="text-blue"><i>Vibrio natriegens</i></span> is a
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| − | rapidly growing bacterial host with one of the fastest growth
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| − | rates of any known organism
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| − | </li>
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| − | <li>
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| − | <strong>Aim</strong>: Improvement of heterologous protein
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| − | expression by
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| − | <span class="text-blue"
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| − | >optimization of tRNA availability of rare codons</span
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| − | >
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| − | </li>
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| − | <li>
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| − | Rare codons are supposed to be a key determining factor for
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| − | protein expression
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| − | </li>
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| − | </ul>
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| − | </div>
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| − | <div class="tile hideme is-child vibrio-bg-img-2">
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| − | <div
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| − | style="padding-top: 17rem;border-bottom: 3px solid #006cbf;"
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| − | class="title"
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| − | >
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| − | <h3>Realization</h3>
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| − | </div>
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| − | <ul>
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| − | <li>
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| − | Analysis of codon usage tables of Vibrio natriegens and
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| − | <span class="text-blue">identification</span> of genes encoding
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| − | for rare tRNAs using tRNA-Scan
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| − | </li>
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| − | <li>
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| − | Development of a
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| − | <span class="text-blue">new vector system</span> with a low copy
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| − | number for inducible expression of tRNA
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| − | </li>
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| − | <li>Verification of improved tRNA availability by qPCR</li>
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| − | <li>
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| − | Verification of improved protein expression by
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| − | <span class="text-blue">expression of modified eGFP</span> and
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| − | <span class="text-blue">proteins</span> with
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| − | <span class="text-blue">high amounts of rare codons</span>
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| − | </li>
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| − | <li>
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| − | Development of <span class="text-blue">GeneCodonSearch</span>, an
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| − | algorithm which identifies model proteins with high amounts of
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| − | rare codons in model organisms (<i>E. coli</i>,
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| − | <i>S. cerevisiae</i>, <i>Arabidopsis thaliana</i>,
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| − | <i>Mus Musculus</i> and <i>Homo sapiens</i>)
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| − | </li>
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| − |
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| − | <li>
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| − | <span class="text-blue"><i>Vibrio natriegens</i></span> is a
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| − | rapidly growing bacterial host with one of the fastest growth
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| − | rates of any known organism
| |
| − | </li>
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| − | <li>
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| − | <strong>Aim</strong>: Improvement of heterologous protein
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| − | expression by
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| − | <span class="text-blue"
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| − | >optimization of tRNA availability of rare codons</span
| |
| − | >
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| − | </li>
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| − | <li>
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| − | Rare codons are supposed to be a key determining factor for
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| − | protein expression
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| − | </li>
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| − | </ul>
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| − | </div>
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| − | <div class="tile hideme is-child">
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| − | <img
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| − | class="hideme"
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| − | src="https://2019.igem.org/wiki/images/thumb/e/e1/T--Stuttgart--index-poster-right-graph.png/800px-T--Stuttgart--index-poster-right-graph.png"
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| − | />
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <div style="display: none">
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| − | <div class="column full_size">
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| − | <img
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| − | src="https://2019.igem.org/wiki/images/5/52/T--Stuttgart--GroupPicture.png"
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| − | />
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| − | </div>
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| − |
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| − | <div class="column full_size">
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| − | <h3>Before you start</h3>
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| − | <p>Please read the following pages:</p>
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| − | <ul>
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| − | <li><a href="https://2019.igem.org/Competition">Competition Hub</a></li>
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| − | <li>
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| − | <a href="https://2019.igem.org/Competition/Deliverables/Wiki"
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| − | >Wiki Requirements page</a
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| − | >
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| − | </li>
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| − | <li>
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| − | <a href="https://2019.igem.org/Resources/Template_Documentation"
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| − | >Template documentation</a
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| − | >
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| − | </li>
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| − | </ul>
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| − | </div>
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| − |
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| − | <div class="clear extra_space"></div>
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| − | <div class="line_divider"></div>
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| − | <div class="clear extra_space"></div>
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| − |
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| − | <div class="column full_size">
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| − | <h3>Styling your wiki</h3>
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| − | <p>
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| − | You may style this page as you like or you can simply leave the style as
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| − | it is. You can easily keep the styling and edit the content of these
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| − | default wiki pages with your project information and completely fulfill
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| − | the requirement to document your project.
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| − | </p>
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| − | <p>
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| − | While you may not win Best Wiki with this styling, your team is still
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| − | eligible for all other awards. This default wiki meets the requirements,
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| − | it improves navigability and ease of use for visitors, and you should
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| − | not feel it is necessary to style beyond what has been provided.
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| − | </p>
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| − | </div>
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| − |
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| − | <div class="clear extra_space"></div>
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| − |
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| − | <div class="column third_size">
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| − | <h3>Uploading pictures and files</h3>
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| − | <p>
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| − | You must upload any pictures and files to the iGEM 2019 server. Remember
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| − | to keep all your pictures and files within your team's namespace or at
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| − | least include your team's name in the file name.
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| − | </p>
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| − |
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| − | <p>
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| − | When you upload, set the "Destination Filename" to
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| − | <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this,
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| − | someone else might upload a different file with the same "Destination
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| − | Filename", and your file would be erased!)
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| − | </p>
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| − |
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| − | <div class="button_link">
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| − | <a href="https://2019.igem.org/Special:Upload">
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| − | UPLOAD FILES
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| − | </a>
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| − | </div>
| |
| − | </div>
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| − |
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| − | <div class="column third_size">
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| − | <h3>Wiki template information</h3>
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| − | <p>
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| − | We have created these wiki template pages to help you get started and to
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| − | help you think about how your team will be evaluated. You can find a
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| − | list of all the pages tied to awards here at the
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| − | <a href="https://2019.igem.org/Judging/Pages_for_Awards"
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| − | >Pages for awards</a
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| − | >
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| − | link. You must edit these pages to be evaluated for medals and awards,
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| − | but ultimately the design, layout, style and all other elements of your
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| − | team wiki is up to you!
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| − | </p>
| |
| − | </div>
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| − |
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| − | <div class="column third_size">
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| − | <div class="highlight decoration_B_full">
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| − | <h3>Editing your wiki</h3>
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| − | <p>
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| − | On this page you can document your project, introduce your team
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| − | members, document your progress and share your iGEM experience with
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| − | the rest of the world!
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| − | </p>
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| − | <p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − |
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| − | <div class="button_link">
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| − | <a
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| − | href="https://2019.igem.org/wiki/index.php?title=Team:Stuttgart&action=edit"
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| − | >
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| − | EDIT PAGE
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| − | </a>
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| − | </div>
| |
| − | </div>
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| − | </div>
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| − |
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| − | <div class="clear extra_space"></div>
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| − | <div class="line_divider"></div>
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| − | <div class="clear extra_space"></div>
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| − |
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| − | <div class="column two_thirds_size">
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| − | <h3>Tips</h3>
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| − | <p>
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| − | This wiki will be your team’s first interaction with the rest of the
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| − | world, so here are a few tips to help you get started:
| |
| − | </p>
| |
| − | <ul>
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| − | <li>
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| − | State your accomplishments! Tell people what you have achieved from
| |
| − | the start.
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| − | </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
| |
| − | <li>
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| − | You have a global audience! Consider the different backgrounds that
| |
| − | your users come from.
| |
| − | </li>
| |
| − | <li>
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| − | Make sure information is easy to find; nothing should be more than 3
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| − | clicks away.
| |
| − | </li>
| |
| − | <li>
| |
| − | Avoid using very small fonts and low contrast colors; information
| |
| − | should be easy to read.
| |
| − | </li>
| |
| − | <li>
| |
| − | Start documenting your project as early as possible; don’t leave
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| − | anything to the last minute before the Wiki Freeze. For a complete
| |
| − | list of deadlines visit the
| |
| − | <a href="https://2019.igem.org/Calendar">iGEM 2019 calendar</a>
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| − | </li>
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| − | <li>Have lots of fun!</li>
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| − | </ul>
| |
| − | </div>
| |
| − |
| |
| − | <div class="column third_size">
| |
| − | <div class="highlight decoration_A_full">
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| − | <h3>Inspiration</h3>
| |
| − | <p>
| |
| − | You can also view other team wikis for inspiration! Here are some
| |
| − | examples:
| |
| − | </p>
| |
| − | <ul>
| |
| − | <li>
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| − | <a href="http://2014.igem.org/Team:SDU-Denmark/">
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| − | 2014 SDU Denmark
| |
| − | </a>
| |
| − | </li>
| |
| − | <li>
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| − | <a href="http://2014.igem.org/Team:Aalto-Helsinki"
| |
| − | >2014 Aalto-Helsinki</a
| |
| − | >
| |
| − | </li>
| |
| − | <li>
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| − | <a href="http://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a>
| |
| − | </li>
| |
| − | <li>
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| − | <a href="http://2014.igem.org/Team:Michigan"> 2014 Michigan</a>
| |
| − | </li>
| |
| − | <li>
| |
| − | <a href="http://2014.igem.org/Team:ITESM-Guadalajara"
| |
| − | >2014 ITESM-Guadalajara
| |
| − | </a>
| |
| − | </li>
| |
| − | <li>
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| − | <a href="http://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a>
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
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| | </html> | | </html> |
