Difference between revisions of "Team:SZU-China/Human Practices"
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| − | <!-- <img class="leftsideimg2" src="https://2019.igem.org/wiki/images/c/c0/T--SZU-CHINA--img-02.png" style="top: 1200px;">--> | + | <!-- <img class="leftsideimg2" src="https://2019.igem.org/wiki/images/c/c0/T--SZU-CHINA--img-02.png" style="top: 1200px;">--> |
<img class="leftsideimg2" src="https://2019.igem.org/wiki/images/c/c0/T--SZU-CHINA--img-02.png" style="top: 2700px;"> | <img class="leftsideimg2" src="https://2019.igem.org/wiki/images/c/c0/T--SZU-CHINA--img-02.png" style="top: 2700px;"> | ||
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| + | <p class="pjudge"> | ||
| + | We conducted a background research on the current status of <span class="myitalic"> Mikania micrantha </span> in Shenzhen and engaged many different stakeholders to promote the progress of the project. Click on the box below to learn more about our effort. | ||
| + | </p> | ||
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| + | </div> | ||
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| + | </div> | ||
<div class="cardbody"> | <div class="cardbody"> | ||
| − | <div class="card"> | + | <div class="card" onclick="window.open('https://2019.igem.org/Team:SZU-China/Human_Practices/1')"> |
<img class="cardimg" src="https://2019.igem.org/wiki/images/a/a3/T--SZU-CHINA--hplogo2.png" alt=""/> | <img class="cardimg" src="https://2019.igem.org/wiki/images/a/a3/T--SZU-CHINA--hplogo2.png" alt=""/> | ||
| − | <div> | + | <div > |
<h3 class="cardheadline">Background research</h3> | <h3 class="cardheadline">Background research</h3> | ||
| − | <!-- <span class="cardtext">Come and see how we made possible the manufacturing of Micrancide as well as the Application</span>--> | + | <!-- <span class="cardtext">Come and see how we made possible the manufacturing of Micrancide as well as the Application</span>--> |
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="card"> | + | <div class="card" onclick="window.open('https://2019.igem.org/Team:SZU-China/Human_Practices/2')"> |
<img class="cardimg" src="https://2019.igem.org/wiki/images/5/53/T--SZU-CHINA--gplog3.png" alt=""/> | <img class="cardimg" src="https://2019.igem.org/wiki/images/5/53/T--SZU-CHINA--gplog3.png" alt=""/> | ||
<div> | <div> | ||
<h3 class="cardheadline" style="margin-top: 50px;">Community</h3> | <h3 class="cardheadline" style="margin-top: 50px;">Community</h3> | ||
| − | <!-- <span class="cardtext">Here is the siRNA sifting program for other researchers to select RNAi molecules for their specific purpose.</span>--> | + | <!-- <span class="cardtext">Here is the siRNA sifting program for other researchers to select RNAi molecules for their specific purpose.</span>--> |
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="card"> | + | <div class="card" onclick="window.open('https://2019.igem.org/Team:SZU-China/Human_Practices/3')"> |
<img class="cardimg" src="https://2019.igem.org/wiki/images/d/db/T--SZU-CHINA--holgo4.png" alt=""/> | <img class="cardimg" src="https://2019.igem.org/wiki/images/d/db/T--SZU-CHINA--holgo4.png" alt=""/> | ||
<div> | <div> | ||
| − | <h3 class="cardheadline" style="margin-top: 50px;"> | + | <h3 class="cardheadline" style="margin-top: 50px;">Experts</h3> |
| − | <!-- <span class="cardtext">Find out how we decided to use the third generation as the final RNAi-based herbicide</span>--> | + | <!-- <span class="cardtext">Find out how we decided to use the third generation as the final RNAi-based herbicide</span>--> |
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="card"> | + | <div class="card" onclick="window.open('https://2019.igem.org/Team:SZU-China/Human_Practices/4')"> |
<img class="cardimg" src="https://2019.igem.org/wiki/images/b/b8/T--SZU-CHINA--hplog5.png" alt=""/> | <img class="cardimg" src="https://2019.igem.org/wiki/images/b/b8/T--SZU-CHINA--hplog5.png" alt=""/> | ||
| − | <h3 class="cardheadline"> | + | <h3 class="cardheadline">Company & partner</h3> |
| − | <!-- <span class="cardtext">Do you want to see how fast M. micrantha grows without control and how efficient Micrancide is?</span>--> | + | <!-- <span class="cardtext">Do you want to see how fast M. micrantha grows without control and how efficient Micrancide is?</span>--> |
</div> | </div> | ||
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| − | + | <p class="pjudge" style="text-align: center"> | |
| − | < | + | We collect a lot of feedback through background research, interviews, and collaborations. These feedbacks advance the iteration and improvement of our products. Check the timeline below to learn more |
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</div> | </div> | ||
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| − | + | <h1 class="CilckMore">Click on the different sections of the image to learn more</h1> | |
| − | + | <!--<div style="width: 100%; margin: 0 auto">--> | |
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| + | aria-hidden="true">× | ||
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| + | <h3 class="modal-title" > | ||
| + | Inspiration | ||
| + | </h3> | ||
| + | </div> | ||
| + | <div class="modal-body"> | ||
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| + | <div > | ||
| + | <p> | ||
| + | <span class="myitalic">Mikania micrantha</span>, a notorious invasive plant, caused great damage to the ecological environment of Shenzhen. As a local iGEM team in shenzhen, we naturally wanted to solve this problem. More details about the inspiration of our project, please click Description to see more. | ||
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<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>We held the Kick off presentation at the beginning of March, and each team member shared their ideas on this year's iGEM competition. Among them, the idea of using synthetic biology to eliminate the famous invasive species - Mikania micrantha in Shenzhen has attracted everyone's attention.</p> | + | <p>We held the Kick-off presentation at the beginning of March, and each team member shared their ideas on this year's iGEM competition. Among them, the idea of using synthetic biology to eliminate the famous invasive species - <span class="myitalic"> Mikania micrantha </span> in Shenzhen has attracted everyone's attention. Then we randomly interviewed some stakeholders on campus and got more information about <span class="myitalic">Mikania micrantha</span> from them.</p> |
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>We had a lot of discussions on environment protection and ecology restoration.</p> | <p>We had a lot of discussions on environment protection and ecology restoration.</p> | ||
<p>After sharing knowledge about invasive species, we were saddened by the destruction of the invasive species to the ecosystem.</p> | <p>After sharing knowledge about invasive species, we were saddened by the destruction of the invasive species to the ecosystem.</p> | ||
| + | <p>The stakeholders, like fruit farmers and property manager, provided more details about the creeper.</p> | ||
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>We decided to develop a bioherbicide for Mikania micrantha</p> | + | <p>We have a preliminary understanding of the harm of<span class="myitalic"> Mikania micrantha.</span></p> |
| + | <p>We decided to develop a bioherbicide for <span class="myitalic">Mikania micrantha</span></p> | ||
| + | <div> | ||
| + | <img style="width: 100%" src="https://2019.igem.org/wiki/images/e/e9/T--SZU-CHINA--jiny1.jpg" alt="" data-action="zoom"> | ||
| + | </div> | ||
</div> | </div> | ||
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The RNAi can specifically silence target genes, and have the potential to develop into a biological herbicide</p> | The RNAi can specifically silence target genes, and have the potential to develop into a biological herbicide</p> | ||
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>We decided to use the most common RNAi molecule- dsRNA, to silence target genes of Mikania micrantha in order to achieve our goal of killing Mikania micrantha specifically. | + | <p>We decided to use the most common RNAi molecule- dsRNA, to silence target genes of <span class="myitalic"> Mikania micrantha </span> in order to achieve our goal of killing <span class="myitalic"> Mikania micrantha </span> specifically. |
</p> | </p> | ||
</div> | </div> | ||
| − | + | <div> | |
| + | <img style="width: 100%" src="https://2019.igem.org/wiki/images/a/a4/T--SZU-CHINA--bs.jpg" alt="" data-action="zoom"> | ||
| + | </div> | ||
</div> | </div> | ||
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<p>We contacted Dr. Fan Wei, a researcher at AGIS-CAAS. He has long been engaged in genomics and bioinformatics research, and has deep knowledge in the field of bioinformatics algorithm development such as genome assembly, gene annotation, and mutation detection. Dr. Fan Wei was very interested in our project and decided to help us.</p> | <p>We contacted Dr. Fan Wei, a researcher at AGIS-CAAS. He has long been engaged in genomics and bioinformatics research, and has deep knowledge in the field of bioinformatics algorithm development such as genome assembly, gene annotation, and mutation detection. Dr. Fan Wei was very interested in our project and decided to help us.</p> | ||
<h3>Input:</h3> | <h3>Input:</h3> | ||
| − | <p>Fan Wei and the institute provided us with the whole genome of Mikania micrantha. These data are currently not publicly available.</p> | + | <p>Fan Wei and the institute provided us with the whole genome of <span class="myitalic"> Mikania micrantha </span>. These data are currently not publicly available.</p> |
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>The whole genome of Mikania micrantha allows us to conduct more comprehensive genetic screening.</p> | + | <p>The whole genome of <span class="myitalic"> Mikania micrantha </span> allows us to conduct more comprehensive genetic screening.</p> |
| − | <p>The whole genome includes genomes of different organs of Mikania micrantha, such as the genome of its leaves. Then we | + | <p>The whole-genome sequence includes genomes of different organs of<span class="myitalic"> Mikania micrantha</span>, such as the genome of its leaves. Then we learned how to find the specific gene that can be used as a target gene to silence.</p> |
| + | <div> | ||
| + | <img style="width: 100%" src="https://2019.igem.org/wiki/images/8/81/T--SZU-CHINA--nongkey1.png" alt="" data-action="zoom"> | ||
| + | </div> | ||
</div> | </div> | ||
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<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p> | + | <p>Dr. Song Bo, researcher, working at AGIS-CAAS, engaged in genomics research, rich in plant genomics research experience.</p> |
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>Suggest that we look for genes based on the way we apply | <p>Suggest that we look for genes based on the way we apply | ||
| Line 1,295: | Line 1,402: | ||
<p>Gives a more efficient genetic screening program</p> | <p>Gives a more efficient genetic screening program</p> | ||
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>We will look for the target gene of the leaves in the transcriptome, as we will spray the leaves of Mikania micrantha with our herbicide.</p> | + | <p>We will look for the target gene of the leaves in the transcriptome, as we will spray the leaves of <span class="myitalic"> Mikania micrantha </span> with our herbicide.</p> |
<p>we used transcriptome analysis with reference genome method to select our target gene from transcriptome data</p> | <p>we used transcriptome analysis with reference genome method to select our target gene from transcriptome data</p> | ||
</div> | </div> | ||
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</button> | </button> | ||
<h3 class="modal-title" > | <h3 class="modal-title" > | ||
| − | Shenzhen Clear Mikania micrantha Co.,Ltd. | + | Shenzhen Clear <span class="myitalic">Mikania micrantha</span> Co.,Ltd. |
</h3> | </h3> | ||
</div> | </div> | ||
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<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>Shenzhen Clear Mikania micrantha Co.,Ltd. Is the developer of the only chemical herbicide on the market for Mikania micrantha. Their product is called Mieweijing, and the active ingredient is 2,4-D. It is a commonly used plant growth regulator.</p> | + | <p>Shenzhen Clear <span class="myitalic">Mikania micrantha</span> Co.,Ltd. Is the developer of the only chemical herbicide on the market for <span class="myitalic">Mikania micrantha</span>. Their product is called Mieweijing, and the active ingredient is 2,4-D. It is a commonly used plant growth regulator.</p> |
| − | <p>They are very interested in our idea of using the RNAi mechanism to eliminate Mikania micrantha and provided us many useful advises.</p> | + | <p>They are very interested in our idea of using the RNAi mechanism to eliminate <span class="myitalic">Mikania micrantha</span> and provided us many useful advises.</p> |
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>Provided us the effect report of their product.</p> | <p>Provided us the effect report of their product.</p> | ||
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</div> | </div> | ||
| + | <div> | ||
| + | <img style="width: 100%;" src="https://2019.igem.org/wiki/images/6/6c/T--SZU-CHINA--ihp221.png" alt="" data-action="zoom"> | ||
| + | </div> | ||
</div> | </div> | ||
<div class="modal-footer"> | <div class="modal-footer"> | ||
| Line 1,414: | Line 1,524: | ||
<div > | <div > | ||
| − | <p>We screened several genes from the transcriptome of Mikania micrantha obtained from the Genomics Institute and designed them into dsRNA sequences.</p> | + | <p>We screened several genes from the transcriptome of <span class="myitalic"> Mikania micrantha </span> obtained from the Genomics Institute and designed them into dsRNA sequences.</p> |
<p>However, in subsequent experiments we found that there are many problems with dsRNA. dsRNA is low in specificity and poorly effective</p> | <p>However, in subsequent experiments we found that there are many problems with dsRNA. dsRNA is low in specificity and poorly effective</p> | ||
<p>We are trying to improve dsRNA and also start looking for alternatives.</p> | <p>We are trying to improve dsRNA and also start looking for alternatives.</p> | ||
| Line 1,447: | Line 1,557: | ||
<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>In order to learn more about the harm of Mikania micrantha, we went to the Neilingding Island Nature Reserve for an investigation.</p> | + | <p>In order to learn more about the harm of <span class="myitalic"> Mikania micrantha </span>, we went to the Neilingding Island Nature Reserve for an investigation.</p> |
<p>Neilingding Island Nature Reserve is one of the smallest national nature reserves in China. It is located on the west side of Shenzhen, on a small island on the Pearl River Estuary, Preserved an intact south Asian subtropical evergreen broad-leaved forest.</p> | <p>Neilingding Island Nature Reserve is one of the smallest national nature reserves in China. It is located on the west side of Shenzhen, on a small island on the Pearl River Estuary, Preserved an intact south Asian subtropical evergreen broad-leaved forest.</p> | ||
<p>There are many kinds of plants on the island, including 619 kinds of vascular plants. Among them, white cassia and wild lychee are the national key protected plants. Wildlife resources are also very rich. There are as many as 900 macaques on the island, as well as key protected animals such as otters, pangolins, black kite, pythons and tiger frogs.</p> | <p>There are many kinds of plants on the island, including 619 kinds of vascular plants. Among them, white cassia and wild lychee are the national key protected plants. Wildlife resources are also very rich. There are as many as 900 macaques on the island, as well as key protected animals such as otters, pangolins, black kite, pythons and tiger frogs.</p> | ||
| − | <p>But in the middle and late last century, for the unknown reasons, Mikania micrantha was introduced into Neilingding Island and spread rapidly on the island. Mikania micrantha entwined tall trees, sheltering low bushes, and killed the main food of the island's macaques- plantains, which caused a sharp decline in the number of wild animals such as macaques on the island. It can be said that the invasion of Mikania micrantha completely changed the harmonious ecosystem on the island.</p> | + | <p>But in the middle and late last century, for the unknown reasons, <span class="myitalic"> Mikania micrantha </span> was introduced into Neilingding Island and spread rapidly on the island. <span class="myitalic"> Mikania micrantha </span> entwined tall trees, sheltering low bushes, and killed the main food of the island's macaques- plantains, which caused a sharp decline in the number of wild animals such as macaques on the island. It can be said that the invasion of <span class="myitalic"> Mikania micrantha </span> completely changed the harmonious ecosystem on the island.</p> |
<p>For this reason, we chose Neilingding Island as a typical Mikania invasive site for background investigation, hoping to get inspiration from it.</p> | <p>For this reason, we chose Neilingding Island as a typical Mikania invasive site for background investigation, hoping to get inspiration from it.</p> | ||
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>Collected the local plants sample of the island</p> | <p>Collected the local plants sample of the island</p> | ||
| − | <p>Understood the harm of Mikania micrantha to wild animals</p> | + | <p>Understood the harm of <span class="myitalic"> Mikania micrantha </span> to wild animals</p> |
| − | <p>Understood the destruction of Mikania micrantha on the ecosystem</p> | + | <p>Understood the destruction of <span class="myitalic"> Mikania micrantha </span> on the ecosystem</p> |
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| Line 1,463: | Line 1,573: | ||
<p>Provided a data foundation for our simulation experiments</p> | <p>Provided a data foundation for our simulation experiments</p> | ||
<p>For more details of Neilingding Island, please see our documentary.</p> | <p>For more details of Neilingding Island, please see our documentary.</p> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | <div> | ||
| + | <img style="width: 100%;" src="https://2019.igem.org/wiki/images/2/25/T--SZU-CHINA--7.1.jpg" alt="" data-action="zoom"> | ||
| + | <img style="width: 100%;" src="https://2019.igem.org/wiki/images/3/31/T--SZU-CHINA--7.2.jpg" alt="" data-action="zoom"> | ||
| + | <img style="width: 100%;" src="https://2019.igem.org/wiki/images/b/b7/T--SZU-CHINA--7.3.jpg" alt="" data-action="zoom"> | ||
| + | |||
| + | |||
| + | |||
</div> | </div> | ||
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</button> | </button> | ||
<h3 class="modal-title" > | <h3 class="modal-title" > | ||
| − | Dr. Pingping Wei （May | + | Dr. Pingping Wei （May) |
</h3> | </h3> | ||
</div> | </div> | ||
| Line 1,496: | Line 1,618: | ||
<p>Dr. Wei Pingping is a Ph.D. student at Shenzhen University, majoring in ecology. She was studying at Neilingding Island and she was quite familiar with the situation of plants on the island.</p> | <p>Dr. Wei Pingping is a Ph.D. student at Shenzhen University, majoring in ecology. She was studying at Neilingding Island and she was quite familiar with the situation of plants on the island.</p> | ||
<h3>Input:</h3> | <h3>Input:</h3> | ||
| − | <p>Provided the distribution diagram of Mikania micrantha on Neilingding Island</p> | + | <p>Provided the distribution diagram of <span class="myitalic"> Mikania micrantha </span> on Neilingding Island</p> |
| − | <p>Explain the principle of biological control method for preventing Mikania micrantha</p> | + | <p>Explain the principle of biological control method for preventing <span class="myitalic"> Mikania micrantha </span></p> |
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
<p>Expanded our background knowledge</p> | <p>Expanded our background knowledge</p> | ||
| − | <p>Understood the harm of Mikania micrantha</p> | + | <p>Understood the harm of <span class="myitalic"> Mikania micrantha </span></p> |
| − | <p>For a detailed interview, please see:</p> | + | <!--<p>For a detailed interview, please see:</p>--> |
| + | <!----> | ||
</div> | </div> | ||
| + | |||
| + | |||
| + | <div> | ||
| + | <img style="width: 100%;" src="https://2019.igem.org/wiki/images/b/b7/T--SZU-CHINA--8.jpg" alt="" data-action="zoom"> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | </div> | ||
| + | |||
| Line 1,533: | Line 1,666: | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
<p>We discussed the existing issues of dsRNA. In the discussion of poor application of dsRNA, one of the team members proposed using nanomaterials to assist dsRNA into plants. This idea gave us some new inspiration.</p> | <p>We discussed the existing issues of dsRNA. In the discussion of poor application of dsRNA, one of the team members proposed using nanomaterials to assist dsRNA into plants. This idea gave us some new inspiration.</p> | ||
| + | |||
| + | |||
<h3>Input:</h3> | <h3>Input:</h3> | ||
| − | <p>We looked for some papers on nanomaterials and RNAi | + | <p>We looked for some papers on nanomaterials and RNAi and finally got one: a self-assembled RNAi nanoparticle can carry a large amount of siRNA and is easily absorbed by cells. |
| + | |||
| + | </p> | ||
| + | |||
| + | |||
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
<p>We began to synthesize our self-designed RNAi nanoparticles.</p> | <p>We began to synthesize our self-designed RNAi nanoparticles.</p> | ||
| Line 1,603: | Line 1,742: | ||
<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>Biologist, a member of the US National Academy of Sciences, Professor of the University of California, Riverside. The main research contents are microRNA and floral organ development, including the use of microRNA to analyze plant developmental mechanisms, and Arabidopsis as a model to study stem cell | + | <p>Biologist, a member of the US National Academy of Sciences, Professor of the University of California, Riverside. The main research contents are microRNA and floral organ development, including the use of microRNA to analyze plant developmental mechanisms, and Arabidopsis as a model to study stem cell regulatory mechanisms. |
| + | </p> | ||
| + | |||
| + | |||
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>She suggested adjusting the concentration ratio of circular DNA to T7 RNA polymerase to increase the synthesis efficiency of RNAi nanoparticles</p> | <p>She suggested adjusting the concentration ratio of circular DNA to T7 RNA polymerase to increase the synthesis efficiency of RNAi nanoparticles</p> | ||
| Line 1,646: | Line 1,788: | ||
<p>Shenzhen Wildlife Protection and Management Office is a department directly under the Shenzhen Urban Administration. It is responsible for the protection and management of forestry resources and forestry environment, and organizes the investigation, monitoring and evaluation of forestry ecological environmental protection. It is also the department responsible for organizing and implementing the prevention, quarantine and forecasting of forestry pests in Shenzhen.</p> | <p>Shenzhen Wildlife Protection and Management Office is a department directly under the Shenzhen Urban Administration. It is responsible for the protection and management of forestry resources and forestry environment, and organizes the investigation, monitoring and evaluation of forestry ecological environmental protection. It is also the department responsible for organizing and implementing the prevention, quarantine and forecasting of forestry pests in Shenzhen.</p> | ||
<h3>Input:</h3> | <h3>Input:</h3> | ||
| − | <p>Provided the distribution diagram of Mikania micrantha in Shenzhen</p> | + | <p>Provided the distribution diagram of <span class="myitalic"> Mikania micrantha </span> in Shenzhen</p> |
| − | <p>Provided the infested area of Mikania micrantha in Shenzhen</p> | + | <p>Provided the infested area of <span class="myitalic"> Mikania micrantha </span> in Shenzhen</p> |
| − | <p>Show us how to monitor the infested area of Mikania micrantha by satellite remote sensing technology</p> | + | <p>Show us how to monitor the infested area of <span class="myitalic"> Mikania micrantha </span> by satellite remote sensing technology</p> |
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>Make us have a more intuitive understanding of the disaster situation of | + | <p>Make us have a more intuitive understanding of the disaster situation of <span class="myitalic"> Mikania micrantha </span> in shenzhen</p> |
| − | <p>The use of GPS positioning and satellite remote sensing to create a distribution map of Mikania micrantha has attracted our interest. We intend to add this function to our drones.</p> | + | <p>The use of GPS positioning and satellite remote sensing to create a distribution map of <span class="myitalic"> Mikania micrantha </span> has attracted our interest. We intend to add this function to our drones.</p> |
</div> | </div> | ||
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<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>Established in 1983, Fairylake Botanical Garden is located in Luohu, Shenzhen. It is a multi-functional landscape garden integrating plant scientific research, rare species preservation, education and sightseeing. The Botanical Garden is also the only professional institution in Shenzhen that conducts basic research in botany and conducts research on the conservation and utilization of plant diversity.</p> | + | <p>Established in 1983, Fairylake Botanical Garden is located in Luohu, Shenzhen. It is a multi-functional landscape garden integrating plant scientific research, rare species preservation, education, and sightseeing. The Botanical Garden is also the only professional institution in Shenzhen that conducts basic research in botany and conducts research on the conservation and utilization of plant diversity. |
| + | </p> | ||
| + | |||
| + | |||
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>Promised us a test field for future experiment</p> | <p>Promised us a test field for future experiment</p> | ||
| Line 1,721: | Line 1,866: | ||
<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>Botanist, deputy director of Fairylake Botanical Garden, secretary of the institute academic committee. He has a very deep understanding of the invasion of Mikania micrantha in Shenzhen | + | <p>Botanist, deputy director of Fairylake Botanical Garden, secretary of the institute academic committee. He has a very deep understanding of the invasion of <span class="myitalic"> Mikania micrantha </span>in Shenzhen and has repeatedly presided over the removal of <span class="myitalic"> Mikania micrantha </span>in the Botanical Garden. |
| + | </p> | ||
| + | |||
<h3>Input:</h3> | <h3>Input:</h3> | ||
| − | <p>The history of Mikania micrantha invading Shenzhen, including invasive land, invasion time, diffusion speed, and historical maximum diffusion area</p> | + | <p>The history of <span class="myitalic"> Mikania micrantha </span> invading Shenzhen, including invasive land, invasion time, diffusion speed, and historical maximum diffusion area</p> |
| − | <p>Provided the botanical characteristics of Mikania micrantha</p> | + | <p>Provided the botanical characteristics of <span class="myitalic"> Mikania micrantha </span></p> |
| − | <p>Provided the damage of Mikania micrantha on the green space in Shenzhen (especially the destruction of the botanical garden)</p> | + | <p>Provided the damage of <span class="myitalic"> Mikania micrantha </span> on the green space in Shenzhen (especially the destruction of the botanical garden)</p> |
<p>He suggested that we should develop our herbicide target to its rapid growth.</p> | <p>He suggested that we should develop our herbicide target to its rapid growth.</p> | ||
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>Expanded our knowledge of Mikania micrantha</p> | + | <p>Expanded our knowledge of <span class="myitalic"> Mikania micrantha </span></p> |
<p>Provided a lot of useful information about the expansion of Mikania</p> | <p>Provided a lot of useful information about the expansion of Mikania</p> | ||
| − | <p>We re-screened the essential metabolism related gene from the transcript of M. micrantha. | + | <p>We re-screened the essential metabolism-related gene from the transcript of M. micrantha. |
| − | For a detailed interview, please see | + | For a detailed interview, please see</p> |
| + | |||
</div> | </div> | ||
| + | |||
| + | |||
| + | <div> | ||
| + | <img style="width: 100%" src="https://2019.igem.org/wiki/images/2/2e/T--SZU-CHINA--14.jpg" alt="" data-action="zoom"> | ||
| + | </div> | ||
| Line 1,766: | Line 1,919: | ||
<div > | <div > | ||
| − | <p>We developed a second-generation product, RNAi nanoparticle, based on the target genes we selected of the first generation. This self-assembled nanoparticle consisting of about half-million small fragment siRNA can be transcribed outside the organism and mass produced. It seems that this time we have found an excellent solution.</p> | + | <p>We developed a second-generation product, RNAi nanoparticle, based on the target genes we selected of the first generation. This self-assembled nanoparticle consisting of about half-million small fragment siRNA can be transcribed outside the organism and mass-produced. It seems that this time we have found an excellent solution.</p> |
| + | |||
| + | |||
<p>However, during the CCIC meetup, our product was pointed out to have a fatal flaw: price. Due to the need of expensive T7 RNA polymerase during the rolling circle transcription, the final price of our products remains unacceptable high and there is no market competitiveness.</p> | <p>However, during the CCIC meetup, our product was pointed out to have a fatal flaw: price. Due to the need of expensive T7 RNA polymerase during the rolling circle transcription, the final price of our products remains unacceptable high and there is no market competitiveness.</p> | ||
<p>Because of this, we decided to transfer the production of RNAi molecules to genetically modified E. coli.</p> | <p>Because of this, we decided to transfer the production of RNAi molecules to genetically modified E. coli.</p> | ||
| Line 1,841: | Line 1,996: | ||
<p>He mentioned some studies have shown that small RNA molecules can be more stable to exist and easy to be absorbed</p> | <p>He mentioned some studies have shown that small RNA molecules can be more stable to exist and easy to be absorbed</p> | ||
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>Applying multiple different RNAi molecules to Mikania micrantha (inspired by Cocktail therapy)</p> | + | <p>Applying multiple different RNAi molecules to <span class="myitalic"> Mikania micrantha </span> (inspired by Cocktail therapy)</p> |
<p>Designed the related program on scanning the etiolation of leaves, as well as the basic plan on building the related model.</p> | <p>Designed the related program on scanning the etiolation of leaves, as well as the basic plan on building the related model.</p> | ||
</div> | </div> | ||
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<div> | <div> | ||
<img style="width: 100%" src="https://2019.igem.org/wiki/images/2/21/T--SZU-CHINA--IHP_9.jpg" alt="" data-action="zoom"> | <img style="width: 100%" src="https://2019.igem.org/wiki/images/2/21/T--SZU-CHINA--IHP_9.jpg" alt="" data-action="zoom"> | ||
| − | <img style="width: 100%" src="https://2019.igem.org/wiki/images/ | + | <img style="width: 100%" src="https://2019.igem.org/wiki/images/4/48/T--SZU-CHINA--fig._15%EF%BC%888%EF%BC%89.png" alt="" data-action="zoom"> |
</div> | </div> | ||
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</div> | </div> | ||
| + | <div> | ||
| + | <img style="width: 100%" src="https://2019.igem.org/wiki/images/1/1c/T--SZU-CHINA--20.JPG" alt="" data-action="zoom"> | ||
| + | </div> | ||
</div> | </div> | ||
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<div > | <div > | ||
<h3>Description:</h3> | <h3>Description:</h3> | ||
| − | <p>In order to help students on our school and citizens to better understand Mikania micrantha, we held a volunteer activity called “Fight GREEN for More Green ” on the Moon Ridge of Shenzhen University's Xili Campus. We led the spontaneous environmental volunteers to remove Mikania micrantha from the seriously affected orchard and taught them to use our applet to identify Mikania micrantha.</p> | + | <p>In order to help students on our school and citizens to better understand <span class="myitalic"> Mikania micrantha </span>, we held a volunteer activity called “Fight GREEN for More Green ” on the Moon Ridge of Shenzhen University's Xili Campus. We led the spontaneous environmental volunteers to remove <span class="myitalic"> Mikania micrantha </span> from the seriously affected orchard and taught them to use our applet to identify <span class="myitalic"> Mikania micrantha </span>.</p> |
<h3>Input:</h3> | <h3>Input:</h3> | ||
<p>Left a lot of comments on our little program</p> | <p>Left a lot of comments on our little program</p> | ||
| − | <p>Provided us with the effect of manually removing Mikania micrantha</p> | + | <p>Provided us with the effect of manually removing <span class="myitalic"> Mikania micrantha </span></p> |
<h3>Alteration:</h3> | <h3>Alteration:</h3> | ||
| − | <p>They identified and located a large number of Mikania micrantha on campus, and created the Mikania micrantha distribution diagram on campus through the applet.</p> | + | <p>They identified and located a large number of <span class="myitalic"> Mikania micrantha </span> on campus, and created the <span class="myitalic"> Mikania micrantha </span> distribution diagram on campus through the applet.</p> |
<p>We conducted drone tests based on the distribution diagram they provided.</p> | <p>We conducted drone tests based on the distribution diagram they provided.</p> | ||
</div> | </div> | ||
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</div> | </div> | ||
| + | |||
| + | </div> | ||
| + | <div class="modal-footer"> | ||
| + | <button type="button" class="btn btn-default" | ||
| + | data-dismiss="modal">Close | ||
| + | </button> | ||
| + | |||
| + | </div> | ||
| + | </div><!-- /.modal-content --> | ||
| + | </div><!-- /.modal-dialog --> | ||
| + | |||
| + | </div><!-- /.modal --> | ||
| + | <div class="modal fade" id="myModal22" tabindex="-1" role="dialog" aria-labelledby="myModalLabel" aria-hidden="true"> | ||
| + | <div class="modal-dialog"> | ||
| + | |||
| + | <div class="modal-content"> | ||
| + | <div class="modal-header"> | ||
| + | <button type="button" class="close" data-dismiss="modal" | ||
| + | aria-hidden="true">× | ||
| + | </button> | ||
| + | <h3 class="modal-title" > | ||
| + | The third generation | ||
| + | </h3> | ||
| + | |||
| + | </div> | ||
| + | <div class="modal-body"> | ||
| + | |||
| + | <div > | ||
| + | <p>We successfully produced Micrancide and applied it by our drone. For more information, welcome to our | ||
| + | <a id="href1" href="https://2019.igem.org/Team:SZU-China/Results">Results</a> and <a id="href1" href="https://2019.igem.org/Team:SZU-China/Application">Application</a>.</p> | ||
| + | |||
| + | </div> | ||
</div> | </div> | ||
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//position on 1166px | //position on 1166px | ||
var defaultMaps = [ | var defaultMaps = [ | ||
| + | [367,103,783,203], | ||
[661,248,1026,353], | [661,248,1026,353], | ||
[257,356,484,443], | [257,356,484,443], | ||
| Line 2,286: | Line 2,477: | ||
[157,3295,476,3393], | [157,3295,476,3393], | ||
[638,3410,1090,3524], | [638,3410,1090,3524], | ||
| − | + | [247,3557,902,3863], | |
// 163,3299,479,3391 | // 163,3299,479,3391 | ||
// | // | ||
Latest revision as of 03:10, 22 October 2019
Integrated
Human Practices
We conducted a background research on the current status of Mikania micrantha in Shenzhen and engaged many different stakeholders to promote the progress of the project. Click on the box below to learn more about our effort.
Background research
Community
Experts
Company & partner
We collect a lot of feedback through background research, interviews, and collaborations. These feedbacks advance the iteration and improvement of our products. Check the timeline below to learn more
Click on the different sections of the image to learn more
