Team:Pasteur Paris/test/Attributions

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Attributions

We would like to thank all the people that contributed to make our project possible this year. We want to express our deepest gratitude to the Institut Pasteur who hosted us and contributed to bring our team together, to all the contributors, may they be for funding, giving material, technical support or advice. Finally, we want to give a special thank you to our PI (Principal Investigator) and our coaches who were always there for us to help, guide and advise us.

Mentors

Although the project is completely elaborated and conducted by the members of the team, this incredible journey would have never been possible without our PI and our coaches. We want to particularly thank them:

Deshmukh Gopaul: PI of the team, he is the reason why iGEM Pasteur could take place once more this year. He assembled the team, supervised it and let its members express the best of themselves to elaborate and carry out this project. Thanks to his experience of the iGEM competition and his contacts we were able to meet a lot of people to help develop and present our project.

Chloe Charendoff (PhD Student): She gave us a great help in the lab thanks to her knowledge in molecular and cellular biology. She allowed us to test a PCR purification kit from Qiagen and a purification by gel extraction kit. She provided us the BET. She also helped during the SELEX experiments and for our proof of concept using flow cytometry. She gave us advice to turn a good presentation into a great one. She also spent time reading and correcting several presentation supports and the wiki content.

Maria Vittoria Mazzuoli(PhD Student): She gave us help, support and advice in the lab and all along the development of our project thanks to her knowledge in biology. She gave us the appropriate culture medium (THY) and helped us for the culture of S. aureus. She provided us the pMK4 plasmid for the OR biobrick and helped us make S. aureus competent with a strain (RN4220) she provided us. She also gave us illumina G-25 microspin GE Healthcare purification columns.

Mathieu de Jode (PhD Student): He helped us all along the development of our project, gave us help and advice in the lab thanks to his knowledge in molecular biology. He helped us choose the bacterial strains, gave us the protocol to make P. aeruginosa become competent and the antibiotic chloramphenicol for the culture of P. aeruginosa once it was transformed with the biobrick. He helped us to characterize our biobrick and gave us advice during the SELEX procedure. President of Stapa (Association of Young Researchers of Institut Pasteur) he gave us the opportunity to present our project during the YRLS (Young Researchers in Life Sciences) Congress and a Stapa talk.

Members of the team

Biology: Tara and Lea elaborated all the protocols in biology and spent all summer doing bacterial cultures and the SELEX method to select all aptamer candidates. They sent the aptamers to be sequenced and analyzed the results. Ronan, Alexandre, Elise and Quentin run bacterial cultures characterization.

Chemistry: Ronan, Laurence and Quentin worked to elaborate the protocols. Ronan and Laurence ordered the electrodes and the necessary reactants. Laurence and Quentin carried out the fixation of the aptamers on the electrodes. Ronan, Laurence and Quentin characterized the electrodes by cyclic voltammetry. Laurence and Quentin realized the detection tests.

Design: Ronan supervised the design of our prototype with the help of Marie, Laurence, Alexandre and Vincent. Laurence, Ronan, Elise and Alexandre realized the design of our different communication supports. We all collaborated to define the visual identity of DIANE.

Wiki: Alexandre (main programmer), Elise and Quentin were responsible of the construction of the wiki and learnt how to design and code a website. All the members of the team contributed to write the content of the wiki.

Fundraising: Tara, Lea, Ronan, Clara, Marie and Vincent worked to find sponsors to fund our project. Quentin and Marie were in charge to create and communicate our crowdfunding campaign on the platform Thellie with the advice and the approval of Deshmukh Gopaul and Clementine Schilte. Quentin contacted several companies in order to try to get private funds. All the members relayed the campaigns on their social networks.

Communication: Marie and Ronan were responsible of the communication on social networks and on the campus of the Institut Pasteur. Quentin and Tara were in charge of all the videos editing.

Entrepreneurship: Ronan, Clara, Marie, Laurence and Vincent were in charge to evaluate the competitors, elaborate a business plan and think of how to make a potential thriving start-up out of our project. Ronan and Clara wrote the whole study of market analysis and entrepreneurship.

Human Practices:
- Survey: Clara created and shared a survey to evaluate the general knowledge of people about antibiotic resistance.
- Events: Tara, Lea and Alexandre were in charge of the organization of the inParis European Meet-up and all the members took part as a member of the staff. Elise and Alexandre worked on the supports to present during the sessions in Cité des sciences et de l’industrie and Ronan, Elise, Alexandre, Vincent, Laurence and Quentin ensured the presentation. Laurence created the visual support for the presentation to middle school students and Ronan, Laurence, Alexandre, Elise and Quentin ensured the presentation. Ronan and Laurence created the poster for the poster sessions during the meet-ups and during YRLS and they also created the fliers. Both of them and Quentin ensured the presentation at the YRLS. Ronan allowed us to present our project during a day of presentation to the general public of many projects run by researchers at the Institut Pasteur and Ronan, Vincent, Marie, Quentin and Alexandre ensured the presentation.
- Safety: Tara, Lea, Clara and Ronan were in charge of the safety part.
- Law/Ethics: Clara and Elise were in charge of all legal and intellectual property aspects of our project. They wrote a comprehensive dissertation on these topics.
- Presentations and poster sessions: All the members contributed at some point to the presentations or poster sessions.

Other contributors

We want to express our deepest gratitude to the other contributors to our project:

Biology:
- Marcel Hollenstein: He helped elaborate our SELEX protocol and gave his expertise on DNA and aptamer synthesis and chemical modifications.
- The Education Center of the Institut Pasteur: They let us access to their lab, equipment and materials during the whole summer. We want to express our gratitude to Hervé Waxin, Director of the Teaching center and the lab technicians Murielle Almoussa, Corinne Fayolle, Angélique Godfin, Guylène K’ouas, Isabelle Lequeutre, Gautier Puime, Abdelhak Sandal, for their support.
- Eurofins Genomics: We ordered them the aptamer libraries and sent our aptamers obtained after the SELEX method to be sequenced.
Chemistry:
- Sophie Griveau: She let us use the material needed to run the cyclic voltammetry and gave us support on the electrochemical aspects of our project.
- Cyrine Slim: She let us use the material needed to run the cyclic voltammetry and gave us support on the electrochemical aspects of our project.
Design:
- Claire Dumont: She trained members of the team in graphic and industrial design and helped to design the prototype of our device and transformed the drawing of our logo into a vectorised one.