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Team:Munich/workspace/Alex

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Team:Munich/workspace/framework

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ALiVE

Analysis of Living Cells by Vesicular Export

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Our greatest glory is not in never falling, but in rising every time we fall.

Confucius

Video

Materials Price for a single reaction (15µl) Price per patient
Buffer + Media $0.19 $0.13
Energy Solution $2.99 $1.99
Phage DNA $0.07 $0.05
Work $0.76 $0.50
Total $4.01 $2.67

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References

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  2. ECDC Data and reports: Antimicrobial resistance and consumption, 2017
  3. Liu, Y.-Y. et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect. Dis. 16, 161–168 (2016).
  4. Barriere, S. L. Clinical, economic and societal impact of antibiotic resistance. Expert Opin. Pharmacother. 16, 151–153 (2015).
  5. Dierig, A., Frei, R. & Egli, A. The fast route to microbe identification: matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Pediatr. Infect. Dis. J. 34, 97–9 (2015).
  6. Ng, E. W. Y., Wong, M. Y. M. & Poon, T. C. W. in 139–175 (Springer, Berlin, Heidelberg, 2013).
  7. Theriot, C. M. et al. Antibiotic-induced shifts in the mouse gut microbiome and metabolome increase susceptibility to Clostridium difficile infection. Nat. Commun. 5, 3114 (2014).
  8. Międzybrodzki, R. et al. Clinical Aspects of Phage Therapy. Adv. Virus Res. 83, 73–121 (2012).
  9. Henry, M., Lavigne, R. & Debarbieux, L. Predicting in vivo efficacy of therapeutic bacteriophages used to treat pulmonary infections. Antimicrob. Agents Chemother. 57, 5961–8 (2013).
  10. Pirnay, J.-P. et al. The Magistral Phage. Viruses 10, 64 (2018).
  11. Gill, J., & Hyman, P. (2010). Phage Choice, Isolation, and Preparation for Phage Therapy. Current Pharmaceutical Biotechnology, 11(1), 2–14.
  12. Fortier, L.-C. & Sekulovic, O. Importance of prophages to evolution and virulence of bacterial pathogens. Virulence 4, 354–365 (2013).
  13. Fauconnier, A. in 253–268 (Humana Press, New York, NY, 2018). doi:10.1007/978-1-4939-7395-8_19
  14. Navarro-Garcia, F. Escherichia coli O104:H4 Pathogenesis: an Enteroaggregative E. coli/Shiga Toxin-Producing E. coli Explosive Cocktail of High Virulence. Microbiol. Spectr. 2, (2014).
  15. Colom, J. et al. Microencapsulation with alginate/CaCO3: A strategy for improved phage therapy. Sci. Rep. 7, 41441 (2017).