Difference between revisions of "Team:Leiden"
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| − | + | <a class="HeaderLogo" href="https://2019.igem.org/Team:Leiden"> | |
| − | + | <img src="https://2019.igem.org/wiki/images/e/e3/T--Leiden--Logo_notext.jpg"> | |
| − | + | </a> | |
| − | + | <div class="BlueBar"> | |
| − | + | <div class="Navbar"> | |
| − | + | <a class="Hambo" onclick="Toggle()"> | |
| − | + | <i class="fa fa-bars fa-3x"></i> | |
| − | </a> | + | </a> |
| − | + | <div class="dropdown" > | |
| − | <div class="dropdown" > | + | <button class="DropButton"> |
| + | <a href="https://2019.igem.org/Team:Leiden/Awards"><i class="fas fa-trophy"></i> Awards</a> | ||
| + | </button> | ||
| + | </div> | ||
| + | <div class="dropdown" > | ||
| + | <button class="DropButton"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Experiments"> <i class="fas fa-vial"></i> Lab</a> | ||
| + | </button> | ||
| + | <div class="DropContent"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Experiments">Experiments</a> | ||
| + | |||
| + | <a href="https://2019.igem.org/Team:Leiden/Notebook">Notebook</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Safety">Safety</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown" > | ||
| + | <button class="DropButton"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Team"><i class="fas fa-users"></i> Team</a> | ||
| + | </button> | ||
| + | <div class="DropContent"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Team">Team</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Attributions">Attributions</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Collaborations">Collaborations</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown" > | ||
| + | <button class="DropButton"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Human_Practices"><i class="fas fa-comments"></i> Human Practices</a> | ||
| + | </button> | ||
| + | <div class="DropContent"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Human_Practices">Human Practices</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Entrepreneurship">Entrepreneurship</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Newsletter">Newsletter</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Public_Engagement">Education &<br>Public Engagement</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Sponsors">Sponsors</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown" > | ||
| + | <button class="DropButton"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Description"><i class="fas fa-flask"></i> Project</a> | ||
| + | </button> | ||
| + | <div class="DropContent"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Description">Description</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Demonstrate">Demonstrate</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Model">Model</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Parts">Parts</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Results">Results</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown" > | ||
| + | <button class="DropButton"> | ||
| + | <a href="https://2019.igem.org/Team:Leiden"><i class="fas fa-home"></i> Home </a> | ||
| + | </button> | ||
| + | </div> | ||
| + | <div id="MobileNav" style="height: 0px;"> | ||
| + | <div class="dropdownHambo" > | ||
<button class="DropButton"> | <button class="DropButton"> | ||
| − | <a href="https://2019.igem.org/Team:Leiden | + | <a href="https://2019.igem.org/Team:Leiden"><i class="fas fa-home"></i> Home </a> |
</button> | </button> | ||
| − | + | </div> | |
| − | + | <div class="dropdownHambo" > | |
| − | + | <button class="DropButton ToggleMenu"> | |
| − | + | <a href="javascript:void(0);"><i class="fas fa-flask"></i> Project</a> | |
| − | + | ||
| − | </div> | + | |
| − | <div class=" | + | |
| − | <button class="DropButton"> | + | |
| − | <a href=" | + | |
</button> | </button> | ||
| − | <div class="DropContent"> | + | <div class="DropContent "> |
| − | <a href="https://2019.igem.org/Team:Leiden/Team">Team</a> | + | <a href="https://2019.igem.org/Team:Leiden/Description">Description</a> |
| − | <a href="https://2019.igem.org/Team:Leiden/ | + | <a href="https://2019.igem.org/Team:Leiden/Demonstrate">Demonstrate</a> |
| − | <a href="https://2019.igem.org/Team:Leiden/ | + | <a href="https://2019.igem.org/Team:Leiden/Model">Model</a> |
| + | <a href="https://2019.igem.org/Team:Leiden/Parts">Parts</a> | ||
| + | <a href="https://2019.igem.org/Team:Leiden/Results">Results</a> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="dropdownHambo " > |
| − | <button class="DropButton"> | + | <button class="DropButton ToggleMenu"> |
| − | + | <a href="javascript:void(0);"><i class="fas fa-comments"></i> Human Practices</a> | |
</button> | </button> | ||
<div class="DropContent"> | <div class="DropContent"> | ||
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<a href="https://2019.igem.org/Team:Leiden/Entrepreneurship">Entrepreneurship</a> | <a href="https://2019.igem.org/Team:Leiden/Entrepreneurship">Entrepreneurship</a> | ||
<a href="https://2019.igem.org/Team:Leiden/Newsletter">Newsletter</a> | <a href="https://2019.igem.org/Team:Leiden/Newsletter">Newsletter</a> | ||
| − | <a href="https://2019.igem.org/Team:Leiden/Public_Engagement">Education & | + | <a href="https://2019.igem.org/Team:Leiden/Public_Engagement">Education & Public Engagement</a> |
<a href="https://2019.igem.org/Team:Leiden/Sponsors">Sponsors</a> | <a href="https://2019.igem.org/Team:Leiden/Sponsors">Sponsors</a> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="dropdownHambo " > |
| − | <button class="DropButton"> | + | <button class="DropButton ToggleMenu"> |
| − | <a href=" | + | <a href="javascript:void(0);"><i class="fas fa-users"></i> Team</a> |
</button> | </button> | ||
<div class="DropContent"> | <div class="DropContent"> | ||
| − | <a href="https://2019.igem.org/Team:Leiden | + | <a href="https://2019.igem.org/Team:Leiden/Team">Team</a> |
| − | + | <a href="https://2019.igem.org/Team:Leiden/Attributions">Attributions</a> | |
| − | + | <a href="https://2019.igem.org/Team:Leiden/Collaborations">Collaborations</a> | |
| − | <a href="https://2019.igem.org/Team:Leiden/ | + | |
| − | <a href="https://2019.igem.org/Team:Leiden/ | + | |
</div> | </div> | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="dropdownHambo " > |
| − | <button class="DropButton"> | + | <button class="DropButton ToggleMenu"> |
| − | <a href=" | + | <a href="javascript:void(0);"><i class="fas fa-vial"></i> Lab</a> |
</button> | </button> | ||
| − | + | <div class="DropContent"> | |
| − | + | <a href="https://2019.igem.org/Team:Leiden/Experiments">Experiments</a> | |
| − | <div class=" | + | |
| − | < | + | <a href="https://2019.igem.org/Team:Leiden/Notebook">Notebook</a> |
| − | + | <a href="https://2019.igem.org/Team:Leiden/Safety">Safety</a> | |
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| − | + | <div class="dropdownHambo" > | |
| − | + | <button class="DropButton ToggleMenu"> | |
| − | + | <a href="https://2019.igem.org/Team:Leiden/Awards"><i class="fas fa-trophy"></i> Awards</a> | |
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<h2>Our project</h2> | <h2>Our project</h2> | ||
<p>Burn wounds are one of the leading causes of morbidity and lead to the life-long disfigurement and disability of millions of people annually. Current treatments still lack necessary features leading to a high demand for alternative treatments. The biomaterial suckerin combines all required characteristics for a wound dressing, due to its unique properties. Our aim was to use this protein to create a hydrogel dressing. The implementation of a cleavable linker system allows for the coupling of antimicrobial peptides and wound healing agents. Thereby, our suckerin-based hydrogel can improve the prognosis for burn wound victims by both preventing infections and promoting wound healing. </p> | <p>Burn wounds are one of the leading causes of morbidity and lead to the life-long disfigurement and disability of millions of people annually. Current treatments still lack necessary features leading to a high demand for alternative treatments. The biomaterial suckerin combines all required characteristics for a wound dressing, due to its unique properties. Our aim was to use this protein to create a hydrogel dressing. The implementation of a cleavable linker system allows for the coupling of antimicrobial peptides and wound healing agents. Thereby, our suckerin-based hydrogel can improve the prognosis for burn wound victims by both preventing infections and promoting wound healing. </p> | ||
| − | <br><h2 style="font-size: 17px;">Click our animations to watch them again!</h2> | + | <br><h2 style="font-size: 17px;">Click on our animations to watch them again!</h2> |
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<div style="overflow: auto; max-height: 30vw;"> | <div style="overflow: auto; max-height: 30vw;"> | ||
<h2>Model</h2> | <h2>Model</h2> | ||
| − | <p> | + | <p>To improve and optimize large scale production of suckerin, we created a model of <em>E. coli</em> metabolism, as well as metabolic pathways, including suckerin-19 production. We also provided a step-by-step guide through the making of our model that can be used by future iGEM teams as a reference. |
| + | </p> | ||
</div> | </div> | ||
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<div style="overflow: auto; max-height: 30vw;"> | <div style="overflow: auto; max-height: 30vw;"> | ||
<h2>Human practices</h2> | <h2>Human practices</h2> | ||
| − | <p> | + | <p>In our project, we interacted with a large variety of stakeholders and combined their feedback with knowledge from previous iGEM teams and literature. This enabled us to make informed decisions regarding the product design of our suckerin-based hydrogel.</p> |
</div> | </div> | ||
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<div style="overflow: auto; max-height: 30vw;"> | <div style="overflow: auto; max-height: 30vw;"> | ||
<h2>Entrepreneurship</h2> | <h2>Entrepreneurship</h2> | ||
| − | <p> | + | <p>Our entrepreneurship program is the result of numerous conversations with potential customers, as well as entrepreneurs within the medical biotechnology field. We constructed a novel <em>three-phase comprehensive roadmap to launch a successful business</em> to serve as a guide for future iGEM teams.</p> |
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<div style="overflow: auto; max-height: 30vw;"> | <div style="overflow: auto; max-height: 30vw;"> | ||
<h2>Public engagement</h2> | <h2>Public engagement</h2> | ||
| − | <p> | + | <p>The public opinion towards applied synthetic biology is predominated by unfamiliarity. Since synthetic biology played an essential role in the development of our product, we strived to make people more aware of the advantages and the ethical regulations concerned in this field, through practical workshops and discussion events.</p> |
</div> | </div> | ||
Latest revision as of 14:13, 23 November 2019
S.P.L.A.S.H.
Suckerin Polymer Layer to Achieve Sustainable Health
S.P.L.A.S.H.
Suckerin Polymer Layer to Achieve Sustainable Health
Our project
Burn wounds are one of the leading causes of morbidity and lead to the life-long disfigurement and disability of millions of people annually. Current treatments still lack necessary features leading to a high demand for alternative treatments. The biomaterial suckerin combines all required characteristics for a wound dressing, due to its unique properties. Our aim was to use this protein to create a hydrogel dressing. The implementation of a cleavable linker system allows for the coupling of antimicrobial peptides and wound healing agents. Thereby, our suckerin-based hydrogel can improve the prognosis for burn wound victims by both preventing infections and promoting wound healing.
Click on our animations to watch them again!
Severe burn wounds constitute a major public health problem causing 300,000 casualties annually. In 42%-65% of cases, these fatalities are caused by bacterial infections. Besides this, 11 million victims a year are hospitalized, many of whom are left with lifelong disfiguration and disabilities.
Donor skin, used in current treatments, is scarce due to specific requirements and many processing steps leading to high demand for alternative treatments. Various materials have been investigated for the production of wound dressings to substitute donor skin. Even though improvements have been made in the form of hydrogels, many features are still missing. The biomaterial suckerin combines all required characteristics for a wound dressing, due to its unique properties and can be used to further optimize hydrogel treatment. Therefore, a suckerin-based hydrogel can improve the treatment of third-degree burn wounds.
Suckerin is originally found in the sucker ring teeth of the Humboldt squid Dosidicus gigas. Suckerin has unique features such as flexibility, strength, and ability to self-assemble into β-sheets, making it ideal for hydrogel formation. As a protein, suckerin can be produced by microorganisms. This opens up possibilities for molecular engineering enabling fast and high-yield production. The iGEM Leiden 2019 team strives to use these assets to produce a suckerin-based hydrogel as a donor skin substitute.
Introducing a cleavable linker system enables the joining of antimicrobial peptides, numbing agents and wound healing stimulators. Therefore, our suckerin-based hydrogel can improve the prognosis for burn wound victims by both preventing infections and promoting wound healing.
Model
To improve and optimize large scale production of suckerin, we created a model of E. coli metabolism, as well as metabolic pathways, including suckerin-19 production. We also provided a step-by-step guide through the making of our model that can be used by future iGEM teams as a reference.
Human practices
In our project, we interacted with a large variety of stakeholders and combined their feedback with knowledge from previous iGEM teams and literature. This enabled us to make informed decisions regarding the product design of our suckerin-based hydrogel.
Entrepreneurship
Our entrepreneurship program is the result of numerous conversations with potential customers, as well as entrepreneurs within the medical biotechnology field. We constructed a novel three-phase comprehensive roadmap to launch a successful business to serve as a guide for future iGEM teams.
Public engagement
The public opinion towards applied synthetic biology is predominated by unfamiliarity. Since synthetic biology played an essential role in the development of our product, we strived to make people more aware of the advantages and the ethical regulations concerned in this field, through practical workshops and discussion events.