Difference between revisions of "Team:Leiden"

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         <title>iGEM Leiden | 2019 </title>
 
         <title>iGEM Leiden | 2019 </title>
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                     <h1><span class="Highlight">iGEM</span> Leiden 2019</h1>
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                     <h1><span class="Highlight">iGEM</span> Leiden <span class="RemoveSmall">2019</span></h1>
 
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                        <a href="#Abstract">Project</a>
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                        <a href="#People">Team</a>
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             <h1>Coming Soon!</h1>
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                    <h1>iGEM Leiden <span class="RemoveSmall">2019</span></h1>
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                </div>
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                <div id="SubTitle">
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                    <h3>Healing burn and chronic wounds using suckerin-based hydrogels
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                    </h3>
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                </div>
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            </div>
 
         </section>
 
         </section>
  
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        <section  class="container text" id="Abstract">
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                <h1>Project description</h1>
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                <p>
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                    Severe burn and chronic wounds are currently the cause of 180.000 deaths per year and the debilitation of millions [1]. Despite this, no efficient treatments are available for these patients. The iGEM Leiden 2019 team thrives to tackle this problem by the use of the recently discovered protein ‘suckerin’ found in the sucker ring teeth of the Humboldt squid <i>Dosidicus gigas</i> [2].
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                </p>
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            <br>
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                <p>
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                    Suckerin occurs in various configurations comprising two main modules, and as a result, the sucker ring teeth come in different strengths and flexibilities. Due to its strong, flexible, moldable, soluble, and self-assembling features and its simple amino acids repeats, suckerin has great promise as a novel biomaterial [3]. In addition, these characteristics make suckerin favourable for efficient expression in microorganisms, enabling efficient throughput production, modification and improvement of the biomaterial.
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                </p>
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            <br>
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                <p>
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                    The potential opportunities that suckerin-based biomaterials provide are overwhelming. Although only a few applications of suckerin-based materials have been explored, the distinct characteristics of suckerin make it highly promising for areas such as the medical or biodegradable plastics sectors. Due to its adjustable elasticity, suckerin can be used for tissue engineering, restorative medicine, drug delivery and cosmetics [4,5]. Applying suckerin as a scaffold, could potentially provide a functional attachment platform of antimicrobial peptides and antihistamines, qualifying it as a promising wound dressing material.
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                </p>
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            <br>
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                <p>
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                    Our aim is to explore the opportunities of this material by preliminary producing various structures of suckerin. Efficient microbial hosts are selected for production improvement and biomaterial characteristics will be monitored for their applicability. Based on the innovative character of the biomaterial, a suckerin-based hydrogel construct will be created that is linked to antimicrobial peptides. Since these linkages are going to be designed to release the antimicrobial peptides upon light exposure or in the presence of bacterial infection, the suckerin-hydrogel offers high potentials in the application of healing burn and chronic wounds.
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                </p>
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        </section>
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        <section id="People" class="container">
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            <div id="Team">
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                <h2>The Team</h2>
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                <div  class="FlexBox"> 
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                        <img src="https://2019.igem.org/wiki/images/9/9f/T--Leiden--Alejandro.JPG">
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                        <div class="box">
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                            <p><b>Alejandro Marquiegui</b> <br>
 +
                                Lab Safety Manager
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                            </p>
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                        </div>
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                    </div>
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                    <div class="person">
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                        <img src="https://2019.igem.org/wiki/images/9/96/T--Leiden--Daan.JPG">
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                        <div class="box">
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                            <p><b>Daan van Tol</b> <br>
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                                Secretary
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                            </p>
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                        </div>
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                    </div>
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                    <div class="person">
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                        <img src="https://2019.igem.org/wiki/images/b/b5/T--Leiden--Daniel.JPG">
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                        <div class="box">
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                            <p> <b>Daniël Tan</b> <br>
 +
                                Treasurer
 +
                            </p>
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                        </div>
 +
                    </div>
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                    <div class="person">
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                        <img src="https://2019.igem.org/wiki/images/c/c5/T--Leiden--Floor.JPG">
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                        <div class="box">
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                            <p> <b>Floor Stel</b> <br>
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                                Human Practices Manager
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                            </p>
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                        </div>
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                    </div>
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                    <div class="person">
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                        <img src="https://2019.igem.org/wiki/images/e/e1/T--Leiden--Joanne.JPG">
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                        <div class="box">
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                            <p><b>Jo-Anne Verschoor</b> <br>
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                                Science Manager
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                            </p>
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                        </div>
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                    </div>
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                    <div class="person">
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                        <img src="https://2019.igem.org/wiki/images/d/d7/T--Leiden--Jonah.JPG">
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                        <div class="box">
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                            <p> <b>Jonah Anderson</b><br>
 +
                                Programming Manager
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                            </p>
 +
                        </div>
 +
                    </div>
 +
                    <div class="person">
 +
                        <img src="https://2019.igem.org/wiki/images/a/ac/T--Leiden--Maarten.JPG">
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                        <div class="box">
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                            <p> <b>Maarten Lubbers</b> <br>
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                                Public Relations Manager
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                            </p>
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                        </div>
 +
                    </div>
 +
                    <div class="person">
 +
                        <img src="https://2019.igem.org/wiki/images/6/60/T--Leiden--Melanie.JPG">
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                        <div class="box">
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                            <p><b>Melanie Ofman</b><br>
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                                Wiki Manager
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                            </p>
 +
                        </div>
 +
                    </div>
 +
                    <div class="person">
 +
                        <img src="https://2019.igem.org/wiki/images/f/f1/T--Leiden--Steijn.JPG">
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                        <div class="box">
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                            <p> <b>Steijn Beekman</b> <br>
 +
                                Fundraising Manager
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                            </p>
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                        </div>
 +
                    </div>
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                    <div class="person">
 +
                        <img src="https://2019.igem.org/wiki/images/c/cc/T--Leiden--Tobi.JPG">
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                        <div class="box">
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                            <p><b>Tobias Fecker</b> <br>
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                                Team Manager
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                            </p>
 +
                        </div>
 +
                    </div>
 +
                    <div class="person">
 +
                        <img src="https://2019.igem.org/wiki/images/8/8c/T--Leiden--Vera.JPG">
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                        <div class="box">
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                            <p> <b>Vera Williams</b> <br>
 +
                                Design Manager
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                            </p>
 +
                        </div>
 +
                    </div>
 +
                </div>
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            </div>
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            <br>
 +
            <br>
 +
            <div id="Advisors">
 +
            <h2>Our principal investigators & advisors</h2>
 +
            <div class="FlexBox">
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                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/8/86/T--Leiden--Carli.JPG">
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                    <div class="box">
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                        <p>
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                            <b>Carli Koster</b>
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                        </p>
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                        <p>
 +
                            Advisor
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                        </p>
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                    </div>
 +
                </div>
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                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/f/f7/T--Leiden--Char.JPG" >
 +
                    <div class="box">
 +
                        <p>
 +
                            <b>Charlotte de Ceuninck</b>
 +
                        </p>
 +
                        <p>
 +
                            Advisor
 +
                        </p>
 +
                    </div>
 +
                </div>
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                <div class="person">
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                    <img src="https://2019.igem.org/wiki/images/6/67/T--Leiden--Chiel.JPG">
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                    <div class="box">
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                        <p>
 +
                            <b>Chiel van Amstel</b>
 +
                        </p>
 +
                        <p>
 +
                            Advisor
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                        </p>
 +
                    </div>
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                </div>
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                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/3/39/T--Leiden--Daphne.JPG">
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                    <div class="box">
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                        <p>
 +
                            <b>Daphne van den Homberg</b>
 +
                        </p>
 +
                        <p>
 +
                            Advisor
 +
                        </p>
 +
                    </div>
 +
                </div>
 +
                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/9/93/T--Leiden--Laurens.JPG">
 +
                    <div class="box">
 +
                        <p>
 +
                            <b>Laurens ter Haar</b>
 +
                        </p>
 +
                        <p>
 +
                            Advisor
 +
                        </p>
 +
                    </div>
 +
                </div>
 +
                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/9/91/T--Leiden--Marjo.JPG">
 +
                    <div class="box">
 +
                        <p>
 +
                            <b>Marjolein Crooijmans</b>
 +
                        </p>
 +
                        <p>
 +
                            Advisor
 +
                        </p>
 +
                    </div>
 +
                </div>
 +
                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/b/b7/T--Leiden--Han.JPG">
 +
                    <div class="box">
 +
                        <p>
 +
                            <b>Han de Winde</b>
 +
                        </p>
 +
                        <p>
 +
                            Principal investigator
 +
                        </p>
 +
                    </div>
 +
                </div>
 +
                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/9/9e/T--Leiden--Dennis.JPG">
 +
                    <div class="box">
 +
                        <p>
 +
                            <b>Dennis Claessen</b>
 +
                        </p>
 +
                        <p>
 +
                            Principal investigator
 +
                        </p>
 +
                    </div>
 +
                </div>
 +
                <div class="person">
 +
                    <img src="https://2019.igem.org/wiki/images/9/97/T--Leiden--Noortje.jpg">
 +
                    <div class="box">
 +
                        <p>
 +
                            <b>Noortje Dannenberg</b>
 +
                        </p>
 +
                        <p>
 +
                            Advisor
 +
                        </p>
 +
                    </div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </section>
 +
 +
        <section class="container" id="AbstractReference">
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            <h4>References</h4>
 +
            <ol class="Reference">
 +
                <li>https://www.who.int/news-room/fact-sheets/detail/burns, visited on May 16, 2019.
 +
                </li>
 +
                <li>Miserez A., Weaver, J. C., Pedersen P. B., Schneeberk T., Hanlon R. T., Kisailus D., Birkedal H. (2009). Microstructural and biochemical characterization of the nanoporous sucker rings from Dosidicus gigas. <i>Advanced Materials.</i> 21 (4): 401−406.
 +
                </li>
 +
                <li>Hiew S. H. et al. (2017). Squid Suckerin Biomimetic Peptides Form Amyloid-like Crystals with Robust Mechanical Properties. <i>Biomacromolecules.</i> 18 (12): 4240-4248
 +
                </li>
 +
                <li>Hiew S. H., and Miserez A. (2017). Squid Sucker Ring Teeth: Multiscale Structure-Property Relationships, Sequencing, and Protein Engineering of a Thermoplastic Biopolymer. <i>ACS Biomaterials Science & Engineering.</i> 3 (5): 680-93.</li>
 +
                <li>Eder M., Amini S., and Fratzl P. (2018). Biological Composites-complex Structures for Functional Diversity. <i>Science.</i> 362 (6414): 543-47.
 +
                </li>
 +
 +
            </ol>
 +
 +
 +
        </section>
  
 
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Revision as of 13:12, 9 June 2019

iGEM Leiden | 2019

iGEM Leiden 2019

iGEM Leiden 2019

Healing burn and chronic wounds using suckerin-based hydrogels

Project description

Severe burn and chronic wounds are currently the cause of 180.000 deaths per year and the debilitation of millions [1]. Despite this, no efficient treatments are available for these patients. The iGEM Leiden 2019 team thrives to tackle this problem by the use of the recently discovered protein ‘suckerin’ found in the sucker ring teeth of the Humboldt squid Dosidicus gigas [2].


Suckerin occurs in various configurations comprising two main modules, and as a result, the sucker ring teeth come in different strengths and flexibilities. Due to its strong, flexible, moldable, soluble, and self-assembling features and its simple amino acids repeats, suckerin has great promise as a novel biomaterial [3]. In addition, these characteristics make suckerin favourable for efficient expression in microorganisms, enabling efficient throughput production, modification and improvement of the biomaterial.


The potential opportunities that suckerin-based biomaterials provide are overwhelming. Although only a few applications of suckerin-based materials have been explored, the distinct characteristics of suckerin make it highly promising for areas such as the medical or biodegradable plastics sectors. Due to its adjustable elasticity, suckerin can be used for tissue engineering, restorative medicine, drug delivery and cosmetics [4,5]. Applying suckerin as a scaffold, could potentially provide a functional attachment platform of antimicrobial peptides and antihistamines, qualifying it as a promising wound dressing material.


Our aim is to explore the opportunities of this material by preliminary producing various structures of suckerin. Efficient microbial hosts are selected for production improvement and biomaterial characteristics will be monitored for their applicability. Based on the innovative character of the biomaterial, a suckerin-based hydrogel construct will be created that is linked to antimicrobial peptides. Since these linkages are going to be designed to release the antimicrobial peptides upon light exposure or in the presence of bacterial infection, the suckerin-hydrogel offers high potentials in the application of healing burn and chronic wounds.

The Team

Alejandro Marquiegui
Lab Safety Manager

Daan van Tol
Secretary

Daniël Tan
Treasurer

Floor Stel
Human Practices Manager

Jo-Anne Verschoor
Science Manager

Jonah Anderson
Programming Manager

Maarten Lubbers
Public Relations Manager

Melanie Ofman
Wiki Manager

Steijn Beekman
Fundraising Manager

Tobias Fecker
Team Manager

Vera Williams
Design Manager



Our principal investigators & advisors

Carli Koster

Advisor

Charlotte de Ceuninck

Advisor

Chiel van Amstel

Advisor

Daphne van den Homberg

Advisor

Laurens ter Haar

Advisor

Marjolein Crooijmans

Advisor

Han de Winde

Principal investigator

Dennis Claessen

Principal investigator

Noortje Dannenberg

Advisor

References

  1. https://www.who.int/news-room/fact-sheets/detail/burns, visited on May 16, 2019.
  2. Miserez A., Weaver, J. C., Pedersen P. B., Schneeberk T., Hanlon R. T., Kisailus D., Birkedal H. (2009). Microstructural and biochemical characterization of the nanoporous sucker rings from Dosidicus gigas. Advanced Materials. 21 (4): 401−406.
  3. Hiew S. H. et al. (2017). Squid Suckerin Biomimetic Peptides Form Amyloid-like Crystals with Robust Mechanical Properties. Biomacromolecules. 18 (12): 4240-4248
  4. Hiew S. H., and Miserez A. (2017). Squid Sucker Ring Teeth: Multiscale Structure-Property Relationships, Sequencing, and Protein Engineering of a Thermoplastic Biopolymer. ACS Biomaterials Science & Engineering. 3 (5): 680-93.
  5. Eder M., Amini S., and Fratzl P. (2018). Biological Composites-complex Structures for Functional Diversity. Science. 362 (6414): 543-47.